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Effect of Peroxiredoxin 1 on the biological function of airway epithelial cells and epithelial-mesenchymal transition

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BIOCELL 2022年第12期46卷 2671-2680页

作者： HUOGEN LIU YUNDI SHI XIN WAN YING LIU HAILIN SHU FENGMING HUANG zhenbin gong LING GU Department of Critical Care Medicine Mindong Hospital Affiliated to Fujian Medical UniversityNingde355000China

Peroxiredoxin 1(PRDX1)participates in tumor cell proliferation,apoptosis,migration,invasion,and the epithelial-to-mesenchymal transition(EMT).This study aimed to investigate the effect of PRDX1 on the EMT of airway epithelial cells stimulated with lipopolysaccharide(LPS)and transforming growth factor-beta 1(TGF-β1).PRDX1 overexpression significantly increased the proliferation and migration of human bronchial epithelial(BEAS-2B)cells,reduced cell apoptosis(p<0.01),and induced EMT and collagen deposition by upregulating the expression of the matrix metallopeptidase(MMP)2,MMP9,α-smooth muscle actin(α-SMA),N-cadherin,vimentin and twist proteins and inhibiting E-cadherin expression(p<0.05).PRDX1 overexpression promoted TGF-β1-mediated inhibition of cell proliferation and migration and significantly enhanced the TGF-β1-induced EMT and collagen synthesis(p<0.05).Knockdown of PRDX1 inhibited cell proliferation,migration,EMT,and collagen synthesis(p<0.01),reversed LPS-mediated inhibition of cell proliferation and migration,and significantly suppressed LPS-induced EMT and collagen synthesis(p<0.01).The result indicating that PRDX1 may be involved in LPS/TGF-1-induced EMT and collagen synthesis in human bronchial epithelial cells.

关键词： Peroxiredoxin 1 Airway epithelial cell Epithelial-to-mesenchymal transition Cell migration Collagen synthesis

Hydrophobic, mechanical, and tribological properties of fluorine incorporated hydrogenated fullerene-like carbon films

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Friction 2013年第4期1卷 350-358页

作者： Li QIANG Bin ZHANG Kaixiong GAO zhenbin gong Junyan ZHANG State Key Laboratory of Solid Lubrication Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000China

Fluorine-incorporated hydrogenated fullerene-like nanostructure amorphous carbon films(F-FLC)were synthesized by employing the direct current plasma enhanced chemical vapor deposition(dc-PECVD)technique using a mixture of methane(CH4),tetra-fluoromethane(CF4),and hydrogen(H2)as the working gases.The effect of the fluorine content on the bonding structure,surface roughness,hydrophobic,mechanical,and tribological properties of the films was systematically investigated using Fourier transform infrared spectroscopy(FTIR),X-ray photoelectron spectroscopy(XPS),Raman analysis,atomic force microscope(AFM),contact angle goniometer,nano-indenter,and reciprocating ball-on-disc tester,respectively.The fluorine content in the films increased from 0 to 2.1 at.%as the CF4 gas flow ratio increased from 0 to 3 sccm,and incorporated fluorine atoms existed in the form of C-FX(X=1,2,3)bonds in the film.The fullerene nanostructure embedded in the hydrogenated amorphous carbon films was confirmed by Raman analysis.The water contact angle was significantly increased because of fluorine doping,which indicates that the hydrophobicity of the carbon films could be adjusted to some extent by the fluorine doping.The hardness and elastic modulus of the films remained relatively high(22 GPa)as the fluorine content increased.Furthermore,the friction coefficient of the carbon films was significantly reduced and the wear resistance was enhanced by fluorine doping.

关键词： fullerene-like carbon film fluorine doping hydrophobicity mechanical properties tribological performance

Adhesion and friction performance of DLC/rubber:The influence of plasma pretreatment

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Friction 2021年第3期9卷 627-641页

作者： Changning BAI zhenbin gong Lulu AN Li QIANG Junyan ZHANG Georgy YUSHKOV Alexey NIKOLAEV Maxim SHANDRIKOV Bin ZHANG Key Laboratory of Science and Technology on Wear and Protection of Materials Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China High Current Electronics Institute Russian Academy of SciencesTomsk 634055Russia

Diamond-like carbon(DLC)films are deposited on rubber surfaces to protect the rubber components,and surface pretreatment of the rubber substrates prior to the film deposition can improve the adhesion between the DLC films and the rubber.Thus,the principal purpose of this work concentrates on determining the effects of argon(Ar),oxygen(O_(2)),nitrogen(N_(2)),and hydrogen(H_(2))plasma pretreatments on the adhesion and friction performance of the DLC films deposited on rubber(DLC/rubber).The results indicated that the Ar plasma pretreatment promoted the formation of a compact layer on the rubber surface.By contrast,massive fillers were exposed on the rubber surface after oxygen or nitrogen plasma pretreatments.Moreover,the typical micrometer-scale patches divided by random cracks were observed on the surface of DLC/rubber,except for the sample pretreated with oxygen plasma.The adhesion of DLC/rubber was found to strengthen with the removal of weak boundary layers and the generation of free radicals on the rubber surface after plasma pretreatment.The tribo-tests revealed that DLC/rubber with O_(2),N_(2),and H_(2) plasma pretreatments cannot achieve optimal friction performance.Significantly,DLC/rubber with Ar plasma pretreatment exhibited a low and stable friction coefficient of 0.19 and superior wear resistance,which was correlated to the high adhesion,good load-bearing of the rubber surface,and the approximate sine function of the surface profile of the DLC film.

关键词： diamond-like carbon(DLC)films rubber plasma pretreatment adhesion friction

Distribution characteristics of total mercury and methylmercury in the topsoil and dust of Xiamen, China

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Journal of Environmental Sciences 2009年第10期21卷 1400-1408页

作者： LIANG Ying YUAN Dongxing LU Min gong zhenbin LIU Xiyao ZFIANG Zhen State Key Laboratory of Marine Environmental Science (Xiamen University) College of Oceanography and Environmental Science Xiamen University Xiamen 361005 China. The Eighth Department Guilin University of Electronic Technology Gailin 541004 China

The levels and distribution of mercury （Hg） species, including total mercury （THg） and methylmercury （MeHg） in the topsoil and dust collected from twenty sampling stations located in different land function areas of Xiamen, China, were investigated. The THg concentrations in topsoil ranged from 0.071 to 1.2 mg/kg, and in dust ranged from of 0.034 to 1.4 mg/kg. For stations where the THg of dust was less than 0.31 mg/kg, THg concentrations in the topsoil were significantly correlated to those in the corresponding dust （r = 0.597, n = 16, P = 0.014）. The MeHg concentrations in topsoil were varied between 0.14 and 5.7 μg/kg. The ratios of MeHg/THg in the topsoil ranged from 0.069% to 0.74%. The range of MeHg concentration in the dust were 0.092-2.3 μg/kg. The ratios of MeHg/THg in the dust were at the same level as those in the topsoil. The MeHg concentrations in both topsoil and dust were linked to corresponding THg concentrations and soil organic matter. Neither THg nor MeHg concentration in the topsoil and dust was obviously linked to the land function.

关键词： total mercury methylmercury topsoil dust Xiamen

单笼和配对饲养对食蟹猴血液学和血清生化、行为学和免疫应激的影响

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实验动物科学 2024年第1期41卷 33-38页

作者：赵琪刘永涛汪溪洁兰艳丽余珍斌龚华李彩云上海益诺思生物技术股份有限公司上海201203 中国医药工业研究总院上海201203

目的考察食蟹猴单笼和配对饲养对于动物的血液学和血生化、行为学和免疫应激指标的影响。方法取约3岁食蟹猴,雌雄各4只,分别单笼和同性别配对饲养后,于适应期、单笼饲养第14天和第21天、配对饲养第14天和第21天采集动物血样进行血液... 详细信息

目的考察食蟹猴单笼和配对饲养对于动物的血液学和血生化、行为学和免疫应激指标的影响。方法取约3岁食蟹猴,雌雄各4只,分别单笼和同性别配对饲养后,于适应期、单笼饲养第14天和第21天、配对饲养第14天和第21天采集动物血样进行血液学、血清生化和免疫应激指标检测;于单笼饲养第14~21天和配对饲养第14~21天进行行为学观察。结果单笼和配对饲养食蟹猴的血液学和免疫应激指标均无明显差异;血清生化方面,与基础值比较食蟹猴配对饲养后的总胆红素(TBIL)有显著性降低,单笼饲养时的乳酸脱氢酶(LDH)降低明显;配对饲养后食蟹猴的正常行为和积极行为分别增加了27.93%和86.10%,异常行为减少26.31%。结论单笼饲养和配对饲养对食蟹猴的血液学及免疫应激指标无明显影响;单笼饲养时动物LDH降低,配对饲养时动物TBIL降低,但均在正常背景值范围内;配对的饲养方式能有效改善食蟹猴的异常行为,提高动物福利。

关键词：食蟹猴单笼饲养配对饲养行为学应激血液学生化

基于网络药理学和分子对接技术探讨雷公藤卵巢毒性的机制

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浙江大学学报（医学版） 2022年第1期51卷 62-72页

作者：王志强宫彩霞李振彬中国人民解放军联勤保障部队第九八〇医院风湿免疫科河北石家庄050082 南京中医药大学第一临床医学院江苏南京210023 石家庄平安医院肾脏病科河北石家庄050012

目的:通过网络药理学和分子对接技术探讨雷公藤卵巢毒性的作用机制。方法:通过中药系统药理数据库及分析平台(TCMSP)和比较毒理基因组学数据库(CTD)收集雷公藤的候选毒性化合物和靶点,从CTD中获得雷公藤潜在的卵巢毒性靶点,并利用STRIN... 详细信息

目的:通过网络药理学和分子对接技术探讨雷公藤卵巢毒性的作用机制。方法:通过中药系统药理数据库及分析平台(TCMSP)和比较毒理基因组学数据库(CTD)收集雷公藤的候选毒性化合物和靶点,从CTD中获得雷公藤潜在的卵巢毒性靶点,并利用STRING数据库对雷公藤卵巢毒性的靶点基因进行分析。用Cytoscape软件构建蛋白质-蛋白质相互作用(PPI)网络,用cytoHubba插件鉴定核心基因。此外,利用R软件对雷公藤卵巢毒性的靶点基因进行基因本体(GO)和京都基因与基因组百科全书(KEGG)富集分析。最后,使用Discovery Studio软件对核心毒性化合物和核心基因进行分子对接验证。结果:共获得9个雷公藤候选毒性化合物和56个潜在的卵巢毒性靶点。网络分析结果,雷公藤甲素、山柰酚和雷公藤红素是雷公藤的关键卵巢毒性化合物,核心卵巢毒性基因包括TP53、MYC、PTEN、MAPK3、MTOR、STAT3、EGFR、KRAS、CDH1和AKT1。GO和KEGG分析显示,雷公藤通过氧化应激、生殖系统发育和功能、细胞周期调节、对内源性激素和外源性刺激的反应、细胞凋亡调节、衰老等途径引起卵巢毒性。分子对接研究显示,雷公藤的3个关键卵巢毒性化合物可与10个核心基因的对接口袋相匹配。结论:雷公藤可能通过作用于10个核心基因和140条信号通路而导致卵巢毒性。

关键词：雷公藤卵巢毒性网络药理学靶点通路

高效液相色谱-柱后在线光化学衍生荧光检测法测定辣椒油中4种苏丹红染料

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色谱 2012年第6期30卷 624-629页

作者：刘珺弓振斌厦门大学近海海洋环境科学国家重点实验室福建厦门361005 厦门大学海洋与环境学院福建厦门361005

建立了在线光化学衍生、荧光检测、高效液相色谱(HPLC)测定辣椒油中苏丹红Ⅰ、Ⅱ、Ⅲ和B的方法。以乙腈-水为流动相,采用梯度洗脱方式在SB-C18色谱柱上分离。用实验室自制的程序控制时间/光强光化学反应器作为在线衍生装置,优化了光衍... 详细信息

建立了在线光化学衍生、荧光检测、高效液相色谱(HPLC)测定辣椒油中苏丹红Ⅰ、Ⅱ、Ⅲ和B的方法。以乙腈-水为流动相,采用梯度洗脱方式在SB-C18色谱柱上分离。用实验室自制的程序控制时间/光强光化学反应器作为在线衍生装置,优化了光衍生反应的条件和荧光检测条件。3种不同加标浓度下,辣椒油样品中4种苏丹红染料的加标回收率为81.3%～100.4%。加标水平为0.8 mg/kg下荧光信号强度的相对标准偏差(RSD,n=6)为2.6%～3.8%。苏丹红Ⅰ、Ⅱ、Ⅲ和B的检出限(LOD)和定量限(LOQ)范围分别为0.009～0.054 mg/kg和0.030～0.181 mg/kg,优于传统的HPLC分离、二极管阵列检测器检测方法。该方法具有简单、灵敏、选择性好的特点,适用于食品样品中苏丹红的常规分析。

关键词：高效液相色谱法在线光化学衍生荧光检测苏丹红染料辣椒油

平地横风环境动车组非定常气动特性的试验研究

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铁道科学与工程学报 2023年第3期20卷 802-811页

作者：崔志国黄尊地周镇斌常宁公衍军中车青岛四方机车车辆股份有限公司山东青岛266111 五邑大学轨道交通学院广东江门529020

在定常横风环境影响下,动车组在平地工况运行的稳定性、舒适性及安全性将会恶化。为了揭示其恶化的机理,开展动车组在平地工况伴随定常横风下运行的风洞试验,得到列车表面压力随时间变化的曲线后,再对列车受到的非定常气动载荷时域特性... 详细信息

在定常横风环境影响下,动车组在平地工况运行的稳定性、舒适性及安全性将会恶化。为了揭示其恶化的机理,开展动车组在平地工况伴随定常横风下运行的风洞试验,得到列车表面压力随时间变化的曲线后,再对列车受到的非定常气动载荷时域特性和频域特性进行分析。风洞测试结果表明:在相同风速和同一风向角下,平地动车组车体表面迎风侧1~8号测点和背风侧9~16号测点,其同侧各测点压力平均值在一定范围内波动,总体相差不大。当风向角为90°时,测点压力的幅值和最值随风速的增大而增大,其平均值与风洞来流风速的二次方成正比,即非定常气动压力振动剧烈,波动幅度明显增大。当合成风风速为60 m/s时,测点气动压力的平均值、最小值和最大值随风向角增大呈现先增大后减小的趋势,呈现正弦函数变化规律;当风向角增加到75°时,出现拐点,即最值点;车体表面两侧测点的幅值随风速的增大而增大,即非定常气动压力振动剧烈,振动幅度明显增大。而横风风速和风向角对非定常气动载荷的主振动频率带的影响不大;车体中部两侧测点的频率峰值均集中在0~18 Hz范围内,主振动频率均集中在0~4 Hz区间内,还明显存在频率为4~6 Hz,6~8 Hz和10~14 Hz的3个频域带。研究结论可为高速动车组、城际动车组的车体设计提供理论依据和数据参考。

关键词：横风环境动车组非定常气动特性试验研究车体设计

固相萃取净化及超高效液相色谱-串联质谱法测定橡胶制品中的13种N-亚硝胺

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色谱 2014年第1期32卷 89-94页

作者：陈婷温裕云欧延弓振斌厦门大学近海海洋环境国家重点实验室福建厦门361102 厦门大学环境与生态学院福建厦门361102 福建远东技术服务有限公司福建泉州362006

建立了固相萃取（SPE）净化、超高效液相色谱-串联质谱（UHPLc—MS／MS）测定橡胶制品中13种N-亚硝胺的方法。样品于密闭萃取瓶中于60℃下用甲醇超声萃取30min，C18固相萃取小柱对萃取液进行净化，经C18色谱柱分离，最后用电喷雾正离子... 详细信息

建立了固相萃取（SPE）净化、超高效液相色谱-串联质谱（UHPLc—MS／MS）测定橡胶制品中13种N-亚硝胺的方法。样品于密闭萃取瓶中于60℃下用甲醇超声萃取30min，C18固相萃取小柱对萃取液进行净化，经C18色谱柱分离，最后用电喷雾正离子（ESI＋）和多重反应监测模式（MRM）对13种N-亚硝胺进行定性、定量测定。实验中对样品前处理、色谱分离条件和质谱检测条件进行了优化。在优化的实验条件下，橡胶样品中添加N-亚硝基二甲胺（NDMA）与N-亚硝基-二乙基胺（NDEA）为500μg／kg、其他组分均为50μg／kg时，各组分的相对标准偏差（RSD，n=7）小于10％；在实际样品中的加标回收率为70．7％-117．0％；方法的检出限（LOD，以10倍标准偏差计）为0．5～500μg／kg。方法可应用于橡胶制品中13种N-亚硝胺的测定。

关键词：固相萃取超高效液相色谱-电喷雾电离串联质谱 N-亚硝胺橡胶制品