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嵌入式超薄碳糊膜电极伏安法同时测定黄嘌呤和次黄嘌呤

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分析化学 2005年第5期33卷 671-674页

作者：汪振辉张卉河南省高校环境科学与工程重点学科开放实验室河南省高校环境科学与工程重点学科开放实验室新乡453002 河南师范大学化学与环境科学学院

以镍铬合金为基体制备了嵌入式超薄碳糊膜电极,研究了黄嘌呤(Xa)和次黄嘌呤(Hxa)在该电极上的电化学行为。该电极对Xa和Hxa具有良好的电化学催化特性。两峰电位相差320mV。其氧化峰电流与Xa和Hxa的浓度在5.0×10-8～8.0×10-5mol/L和7.0... 详细信息

以镍铬合金为基体制备了嵌入式超薄碳糊膜电极,研究了黄嘌呤(Xa)和次黄嘌呤(Hxa)在该电极上的电化学行为。该电极对Xa和Hxa具有良好的电化学催化特性。两峰电位相差320mV。其氧化峰电流与Xa和Hxa的浓度在5.0×10-8～8.0×10-5mol/L和7.0×10-8～7.0×10-5mol/L范围内呈良好线性关系;检出限为2.0×10-10mol/L。电极制备简单,有良好的灵敏度、选择性和稳定性。该方法可用于人尿中Xa和Hxa的同时测定。

关键词：嵌入式超薄碳糊膜电极黄嘌呤次黄嘌呤

Genome-wide Identification and Analysis of MVD Gene Family in Euphorbiaceae Plants

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Agricultural Biotechnology 2013年第6期2卷 1-6,11页

作者： Zhi ZOU Lifu YANG Feng AN zhenhui wang Kun YUAN Key Laboratory of Biology and Genetic Resources of Rubber Tree Ministry of Agriculture/Rubber Research InstituteChinese Academy of Tropical Agricultural Sciences

The mevalonate diphosphate deearboxylase （MVD） is an essential enzyme in mevalonate （MVA） pathway that catalyzes the irreversible Mg2＋ -ATP de- pendent decarboxylation of 6-carben compound mevalonate-5-diphosphate （MVAPP） into 5-carbon isopentenyl diphosphate （ IPP）, the building block of sterol and isoprenoid biosynthesis. In this study, based on the published geanme sequences and ESTs, a genome-wide search was carried out for the first time to identify MVD gene family in four genome-sequenced Euphorbiaceae plants, i.e. castor bean （ Ricinus communis）, physic nut （ Jatropha curcas）, cassava （Manihot esculenta） and rubber tree （Hevea brasiliensis）, and to analyze the gene structure and phylogenetic characteristics. According to the experimental results, 1, 1,2 and 2 MVD genes, which all contain 9 introns, were identh＇ied from castor bean, physic nut, cassava and rubber tree, respectively. Homology analysis indicates that MVD genes are widely distributed in eukaryotes, some archaea and eubacteria, which suggests an early origin of this gerte family. Although MVD genes were identified in most green plants, no homologous genes were found in unicellular green algae. In most genome-sequenced plants including castor bean and physic nut, a single copy of MVD gene was found; however, in cassava and rubber tree, two copies were identified just like that in moss, maize, Arabidopsis and poplar. ＂In castor bean, digital expression profiling suggests that in five examined tissues, i.e. leaf, flower, II/III stage endosperm, V/VI stage endosperm and seed, RcPMK was expressed strongly in flower and II/III stage endosperm, moderately in V/VI stage endosperm and leaf, and weakly in seed.

关键词： Euphorbiaceae plants Genome-wide MVD gene family Identification Analysis

Determination of the Correlation between the Air Temperature Measured <i>in Situ</i>and Remotely Sensed Data from MODIS and SEVIRI in Congo-Brazzaville

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Atmospheric and Climate Sciences 2018年第2期8卷 192-211页

作者： Maixent Olivier C. Kambi zhenhui wang Georges Gulemvuga Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters CMA Key Laboratory for Aerosol-Cloud-Precipitation Nanjing University of Information Science & Technology Nanjing China School of Atmospheric Physics Nanjing University of Information Science & Technology Nanjing China Water Resources Management International Commission of Congo-Ubangi-Sangha Basin D. R. of Congo

This study compared data from the MODerate-resolution Imaging Spectroradiometer (MODIS) onboard NASA’s Terra satellite and the Spinning Enhanced Visible and Infrared Imager (SEVIRI) on EUMETSAT’s Meterosat Second Generation (MSG) satellite with in situ data obtained from ground observation stations in Congo-Brazzaville. Remote sensing instruments can be used to estimate air temperature, which has an important role in monitoring the effects of climate change. Congo-Brazzaville is located in equatorial forest, which is difficult to access, and has a limited number of ground meteorological stations measuring air temperature. This study used MODIS and MSG data for the period 2009-2014 to assess the performance of land surface temperature data from satellites against in situ data from ground-based stations in Congo-Brazzaville using a linear regression model. This work has allowed us to determine which satellite is best adapted for use in Central Africa.

关键词： Air Temperature Land Surface Temperature MODIS MSG Tropical Climate Central Africa

β-integrin mediates LPS-induced coelomocytes apoptosis in sea cucumber Apostichopus japonicus via the integrin/FAK/caspase-3 signaling pathway

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β-integrin mediates LPS-induced coelomocytes apoptosis in s...

Molecular Cloning and Analysis of LEA14L2 Gene from Rubber Tree( Hevea brasiliensis Muell. Arg. )

浙江省动物学会第十三次会员代表大会暨学术研讨会

作者： zhenhui wang Chenghua Li Yina Shao School of Marine Sciences Ningbo University

Our previous work indicated LPS could induce apoptosis of coelomocytes in Apostichopus japonicus and P-integrin served as a apoptotic inhibitor during this *** evidence indicated that the integrin/focal adhesion kinase(FAK)signaling cascade modulated the occurrence of apoptosis in *** the present study,a novel FAK was identified from *** by integrin-mediated GST-pull down *** interaction was further validated in the LPS-exposed coelomocytes by immunoprecipitation and immunofluorescence *** better address the functional role of AjFAK in AjITGB-mediated coelomocytes apoptosis,the full-length cDNA of AjFAK was cloned,and its expression relationship with AjITGB was characterized by real-time *** mRNA expression levels of AjFAK decreased to0.48-fold at 12 h in LPS-exposed colemocytes and 0.22-fold at 96 h in Vibrio splendidus challenged sea cucumber,which exhibited consistent expression patterns with that of AjITGB in our previous ***,the expression level of AjFAK decreased to 0.35-fold in AjITGB knockdown treatment by specific *** further assay the apoptotic rate of coelomocytes in AjITGB,AjFAK and AjITGB/AjFAK silencing conditions,and found that the apoptotic percentages were all promoted with 26%,25% and 30% increase compared to that of the control,***,the expression levels of four of caspases from *** were also investigated to determine the apopotic *** silencing AjITGB or AjFAK,the mRNA levels of caspase-3 increased to 6.6-fold and 2.5-fold compared to those of the control,***,the enzymatic activity of caspase-3 was enhanced to 1.82-fold and 1.79-fold compared to control in the two ***,no significant changes were detected in caspase-2/6/*** our results supported thatβ-integrin mediated LPS-induced coelomocytes apoptosis in sea cucumber via integrin/FAK/caspase-3 signaling pathway.

关键词： Apostichopus japonicas lipopolysaccharides integrin FAK caspase-3 apoptosis

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Agricultural Biotechnology 2013年第5期2卷 4-8页

Using an isotig encoding a putative polypeptide with high similarity to Arabidopsis LEA14 as a query, a 613 bp long cDNA was in silico cloned from the transeriptome data of rubber tree. The sequence nominated as HbLEA14L2 contains an ORF of 456 bp with 3 bp 5＇ UTR and 154 bp 3＇ UTR. Subsequently, a 464 bp eDNA and an 834 bp genome sequence containing this ORF was amplified and sequenced. Sequence analysis suggested that HbLEA14L2 has one intron and encodes 151 amino acids with a theoretical molecular weight of 16.55 kDa, isoleetric point of 4.93 and GRAVY value of -0.022, indicating a cytoplasmle localization pattern; HbLEA14L2 protein contains a conserved LEA_2 domain and belongs to LEA_2 subfamily, sharing 91%, 76%, 75%, 72% and 63% similarity with the homologous proteins in castor bean, leafy spurge, poplar, cotton, and Arabidopsis, respectively. Swiss-Model indicated that the tertiary structure of HbLEA14L2 is consisted of one α-helix and seven β-sheets, which was proposed to serve as a regulatory protein to prevent cellular desiccation.

关键词： Hevea brasiliensis Muell. Arg. Arabidopsis thaliana LEA14 Shoot apex

Evaluation and Correction of Ground-Based Microwave Radiometer Observations Based on NCEP-FNL Data

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Atmospheric and Climate Sciences 2019年第2期9卷 229-242页

作者： Qing Li Ming Wei zhenhui wang Yanli Chu Lina Ma Nanjing University of Information Science & Technology Nanjing China

Consistency between the brightness temperatures observed with a ground-based microwave radiometer and the brightness temperatures computed by forward modeling is important in many different data applications. Using the National Centers for Environmental Prediction-Final Operational Global Analysis (NCEP-FNL) dataset as a reference, the brightness temperature was obtained through the radiation transfer model for forward calculation. The problem of segmented features in long time of observational data from ground-based microwave radiometers (the so-called “jumping problem”) was identified. By analyzing the deviation and correlation between the observational bright temperature data and the forward calculated data under clear sky conditions, a revised scheme is proposed for the bright temperature observational data. Data obtained with a ground-based microwave radiometer in Beijing from January 1, 2010 to December 31, 2011 around the date of liquid nitrogen calibration show that the correlation between the observed brightness temperatures and the forward computed brightness temperatures is better after correction, especially at 28 and 30 GHz. The “jumping” problem in the observational data for the brightness temperature is eliminated after correction and the time continuity of the observational data and its consistency with the forward calculated data based on the NCEP-FNL dataset are improved. The proposed correction scheme can be used both for real-time data quality control and to improve the accuracy of historical datasets obtained with poorly calibrated microwave radiometers or radiometers working in polluted environments.

关键词： Atmospheric Remote Sensing Data Correction Forward Model Regression

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审判阶段补充侦查制度反思与改革

浙江工商大学学报 2022年第1期 55-63页

作者：王贞会中国政法大学诉讼法学研究院北京100088

以审判为中心的刑事诉讼制度改革是一项精细化的系统工程,任何一项具体制度的改革完善都有着“牵一发而动全身”的效果。补充侦查的目的在于弥补前期侦查疏漏,完善案件事实和证据。法庭审判阶段补充侦查是程序倒流的典型样态,导致侦查... 详细信息

以审判为中心的刑事诉讼制度改革是一项精细化的系统工程,任何一项具体制度的改革完善都有着“牵一发而动全身”的效果。补充侦查的目的在于弥补前期侦查疏漏,完善案件事实和证据。法庭审判阶段补充侦查是程序倒流的典型样态,导致侦查行为与审判程序交错,渲染了侦查中心主义色彩,与程序安定、程序构造、公正审判等诉讼原理冲突,缺乏理论证成。实践中,这一制度呈现功能异化倾向,成为延长诉讼期限、规避控诉风险和终结诉讼程序的变通之策,背离立法初衷。从长远来看,宜于废止法庭审判阶段的补充侦查,同时建立健全检察引导侦查、公诉审查、撤回起诉、司法业务考评等配套制度,以契合审判中心之要求和庭审实质化之价值。

关键词：补充侦查以审判为中心检察引导侦查撤回起诉

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未成年人严格限制适用逮捕措施的现状调查

国家检察官学院学报 2019年第4期27卷 56-73页

作者：王贞会中国政法大学诉讼法学研究院

严格限制适用逮捕措施是未成年人刑事诉讼的一项原则要求。检察机关对这一规定的落实情况总体较好,有的地区还进行了一定的改革创新,未成年人逮捕数量逐步下降,非羁押比例整体上升。调查也发现一些问题有待解决。应当实现审查逮捕阶段... 详细信息

严格限制适用逮捕措施是未成年人刑事诉讼的一项原则要求。检察机关对这一规定的落实情况总体较好,有的地区还进行了一定的改革创新,未成年人逮捕数量逐步下降,非羁押比例整体上升。调查也发现一些问题有待解决。应当实现审查逮捕阶段听取辩护律师意见的全覆盖;完善逮捕社会危险性证明和审查评估机制;落实对被逮捕未成年人的分押分管和分别教育原则;加强对被逮捕未成年人的羁押必要性审查;完善未成年人非羁押的家庭监护和社会支持体系。

关键词：未成年人严格限制适用逮捕社会危险性评估羁押必要性审查社会观护体系

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法制度功能运行的系统维度与路径实现:以刑事强制措施为例的分析

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社会科学战线 2022年第11期 193-199页

作者：王贞会中国政法大学诉讼法学研究院北京100088

在社会学研究中,功能主义是将某一社会现象或者社会制度置于相互联系的社会系统内进行规范分析的思维路径和方法。功能主义为考察司法制度的运行实效提供了多元视角,可以从社会、法律和司法等不同的系统维度和路径来对司法制度的功能进... 详细信息

在社会学研究中,功能主义是将某一社会现象或者社会制度置于相互联系的社会系统内进行规范分析的思维路径和方法。功能主义为考察司法制度的运行实效提供了多元视角,可以从社会、法律和司法等不同的系统维度和路径来对司法制度的功能进行分层考察。以刑事强制措施为例,它作为一项具体的司法制度具有保证刑事诉讼顺利进行和保障人权的基础性功能,并通过与其他法律制度进行功能整合而实现教育、指引等法律功能和推进社会治理等社会功能。

关键词：刑事司法功能主义强制措施法律实施社会治理

维普期刊数据库