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Journal of Integrative Plant Biology 2009年第1期51卷 107-112页

作者： Yan Hu Wang-zhen Guo Tian-zhen zhang National Key Laboratory of Crop Genetics & Germplasm Enhancement Cotton Research Institute Nanjing Agricultural University Nanjing 210095 China

A bacterial artificial chromosome （BAC） library was constructed for Gossypium hirsutum acc. TM-1, a genetic and genomic standard line for Upland cotton. The library consists of 147 456 clones with an average insert size of 122.8 kb ranging from 97 to 240 kb. About 96.0% of the clones have inserts over 100 kb. Therefore, this library represents theoretically 7.4 haploid genome equivalents based on an AD genome size of 2 425 Mb. Clones were stored in 384 384- well plates and arrayed into multiplex pools for rapid and reliable library screening. BAC screening was carried out by four-round poiymerase chain reactions using 23 simple sequence repeats （SSR） markers, three sequence-related amplified polymorphism markers and one pair of primers for a gene associated with fiber development to test the quality of the library. Correspondingly, in total 92 positive BAC clones were identified with an average four positive clones per SSR marker, ranging from one to eight hits. Additionally, since these SSR markers have been localized to chromosome 12 （A12） and 26 （D12） according to the genetic map, these BAC clones are expected to serve as seeds for the physical mapping of these two homologous chromosomes, sequentially map-based cloning of quantitative trait loci or genes associated with important agronomic traits.

关键词： bacterial artificial chromosome library polymerase chain reaction screening simple sequence repeats markers TM-1 upland cotton.

Uncertainty evaluation and correlation analysis of single-particle energies in phenomenological nuclear mean field:an investigation into propagating uncertainties for independent model parameters

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Nuclear Science and Techniques 2021年第2期32卷 45-57页

作者： zhen-zhen zhang Hua-Lei Wang Hai-Yan Meng Min-Liang Liu School of Physics and Microelectronics Zhengzhou UniversityZhengzhou 450001China Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000China

Based on the Monte Carlo approach and conventional error analysis theory,taking the heaviest doubly magic nucleus 208Pb as an example,we first evaluate the propagated uncertainties of universal potential parameters for three typical types of single-particle energy in the phenomenological Woods–Saxon mean *** the Woods–Saxon modeling with uncorrelated model parameters,we found that the standard deviations of singleparticle energy obtained through the Monte Carlo simulation and the error propagation rules are in good *** seems that the energy uncertainty of the single-particle levels regularly evoluate with certain quantum numbers to a large extent for the given parameter ***,the correlation properties of the single-particle levels within the domain of input parameter uncertainties are statistically analyzed,for example,with the aid of Pearson’s correlation *** was found that a positive,negative,or unrelated relationship may appear between two selected single-particle levels,which will be extremely helpful for evaluating the theoretical uncertainty related to the single-particle levels(e.g.,K isomer)in nuclear structural calculations.

关键词： Uncertainty propagation Correlation effect Woods-Saxon potential

Construction and Identification of Bacterial Artificial Chromosome Library for 0-613-2R in Upland Cotton

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Journal of Integrative Plant Biology 2006年第2期48卷 219-222页

作者： Jian-Mei Yin Wang-zhen Guo Tian-zhen zhang State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China

A bacterial artificial chromosome （BAC） library containing a large genomlc DNA insert is an important tool for genome physical mapping, map-based cloning, and genome sequencing. To Isolate genes via a map-based cloning strategy and to perform physical mapping of the cotton genome, a high-quality BAC library containing large cotton DNA Inserts Is needed. We have developed a BAC library of the restoring line 0-613-2R for Isolating the fertility restorer （Rf1） gene and genomic research in cotton （Gossypium hirsutum L.）. The BAC library contains 97 825 clones stored In 255 pieces of a 384-well mlcrotiter plate. Random samples of BACs digested with the Notl enzyme Indicated that the average Insert size Is approximately 130 kb, with a range of 80-275 kb, and 95.7% of the BAC clones in the library have an average insert size larger than 100 kb. Based on a cotton genome size of 2 250 Mb, library coverage is 5.7 × haploid genome equivalents. Four clones were selected randomly from the library to determine the stability of the BAC clones. There were no different fingerprints for 0 and 100 generations of each clone digested with Notl and Hlndiii enzymes. Thus, the atabiiity of a single BAC clone can be sustained at iesat for 100 generations. Eight simple sequence repeat （SSR） markers flanking the Rf; gene were chosen to screen the BAC library by pool using PCR method and 25 positive clones were identified with 3.1 positive clones per SSR marker.

关键词： bacterial artificial chromosome library cotton map-based cloning Rf gene.

Probes of axial and nonaxial hexadecapole deformation effects in nuclei around^(230)U

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Communications in Theoretical Physics 2023年第2期75卷 133-143页

作者： Zhuo Song Hua-Lei Wang zhen-zhen zhang Min-Liang Liu School of Physics and Microelectronics Zhengzhou UniversityZhengzhou 450001China Key Laboratory of High Precision Nuclear Spectroscopy Institute of Modern PhysicsChinese Academy of SciencesLanzhou 730000China School of Nuclear Science and Technology University of Chinese Academy of SciencesBeijing 100049China

The structure properties for even–even nuclei around^(230)U,located on the hexadecapoledeformation island,are investigated using the potential-energy-surface calculation within the framework of the macroscopic-microscopic *** impact of different deformation degrees of freedom(including axial and nonaxial quadrupole and hexadecapole deformations)on total energy,shell,and pairing contributions is analyzed,based on the projected energy maps and *** single-particle structure is presented and briefly *** a large extent,a much better agreement with experimental data and other theoretical results is obtained if the hexadecapole deformations,especially the axial one,are taken into *** results could provide useful insights into understanding the effects of different quadrupole and hexadecapole deformations.

关键词： hexadecapole deformation triaxial deformation even-even nuclei potential-energysurface calculation macroscopic−microscopic model

Laser-induced breakdown spectroscopy in Asia

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Frontiers of physics 2016年第6期11卷 231-255页

作者： zhen-zhenWang Yoshihiro Deguchi zhen-zhen zhang Zhe Wang Xiao-Yan Zeng Jun-Jie Yan State Key Laboratory of Multiphase Flow in Power Engineering Xi'an Jiaotong University Xi'an 710049 China Graduate School of Advanced Technology and Science Tokushima University Tokushima 770-8501 Japan State Key Lab of Power Systems Department of Thermal Engineering Tsinghua-BP Clean Energy Center Tsinghua University Beijing 100084 China Wuhan National Laboratory for Optoelectronics (WNLO) Huazhong University of Science and Technology Wuhan ~3007~ China

Laser-induced breakdown spectroscopy (LIBS) is an analytical detection technique based on atomic emission spectroscopy to measure the elemental composition. LIBS has been extensively studied and developed due to the non-contact, fast response, high sensitivity, real-time and multi-elemental detection features. The development and applications of LIBS technique in Asia are summarized and discussed in this review paper. The researchers in Asia work on different aspects of the LIBS study in fundamentals, data processing and modeling, applications and instrumentations. According to the current research status, the challenges, opportunities and further development of LIBS technique in Asia are also evaluated to promote LIBS research and its applications.

关键词： laser-induced breakdown spectroscopy (LIBS) quantitative analysis signal enhancement applications challenges

Revisiting the island of hexadecapoledeformation nuclei in the A≈150 mass region:focusing on the model application to nuclear shapes and masses

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Communications in Theoretical Physics 2024年第2期76卷 74-88页

作者： Xiao-Yang Wei Hua-Lei Wang zhen-zhen zhang Min-Liang Liu School of Physics and Microelectronics Zhengzhou UniversityZhengzhou 450001China Key Laboratory of High Precision Nuclear Spectroscopy Institute of Modern PhysicsChinese Academy of SciencesLanzhou 730000China School of Nuclear Science and Technology University of Chinese Academy of SciencesBeijing 100049China

Based on the potential-energy-surface calculation,the impact of different deformation degrees of freedom on a single-particle structure and binding energies in nuclei around^(152)Nd,located on one of the hexadecapole-deformation islands,is analyzed in a multi-dimensional deformation *** energy maps,curves and tables are presented to indicate nuclear *** calculated equilibrium deformations and binding energies with different potential parameters are compared with experimental data and other *** is found that the inclusion of the hexadecapole deformations,especially the axial one,can improve the theoretical description of both nuclear shapes and *** addition,our calculated potential-energy curve shows that a critical deformation-point,β_(2)≈0.4,exists—the triaxial(hexadecapole)deformation effect can be neglectable but the hexadecapole(triaxial)one plays an important role before(after)such a critical point.

关键词： hexadecapole deformation binding energy potential-energy-surface calculation nuclear property

Is XMRV a causal virus for prostate cancer？

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Asian Journal of Andrology 2011年第5期13卷 698-701页

作者： zhen-zhen zhang Bao-Feng Guo Zhuang Feng Ling zhang Xue-Jian Zhao Department of Epidemiology Michigan State University East Lansing MI 48224 USA Research Center of Prostate Diseases Department of Reproductive Pathophysiology School of Basic Medicine Jilin University Changchun 130021 China Department of Surgery China-Japan Union Hospital Norman Bethune Medical School Jilin University Changchun 130031 China Department of Pathology Wayne State University Detroit MI 48201 USA

The potential association between xenotropic murine leukaemia virus-related gammaretrovirus （XMRV） and prostate cancer （PCa） has been documented since 2006, It is important for furthering our understanding of the biological mechanisms of PCa to ascertain whether this association is causal. To summarize the available information on the epidemiological and laboratory findings of the association, we conducted a literature search of the PubMed electronic database （from March 2006 to February 2011） to identify relevant published studies that examined the association between XMRV and PCa, Although several studies showed the positive association between XMRV and PCa, more recent studies did not support this conclusion, The positive findings might be due to contamination of human samples, Further studies are needed to clarify this association,

关键词： prostate cancer review XMRV

MicroRNA Expression Signature in Gastric Cancer

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Chinese Journal of Cancer Research 2009年第1期21卷 74-80页

作者： Hong-chun Luo zhen-zhen zhang Xia zhang Bo Ning Jin-jun Guo Na Niel Bo Liu Xiao-ling Wu Department of Laboratory Medicine Chongqing Medical University Chongqing 400016 China The Key Laboratory for Molecular Biology of Infectious Diseases （Ministry of Education）. The for Viral Hepatitis Chongqing University of Medical Science Chongqing 400016 China Department of Gastroenterology the Second Affiliated Hospital Chongqing University of Science Chongqing 400010 China Institue Medical

Objective： To identify the miRNA specific signature as novel diagnostic and prognostic tools for gastric cancer. Methods： miRNAs expression profiling of 3 normal gastric tissues, 24 malignant tissues, gastric cancer cell SGC7901 and normal gastric cell GES-1 were detected using microarray technology. The hierarchical clustering algorithm of the Cluster software was used to analyse the miRNAs expression of all samples. The expression levels of miR-433 and miR-9 which were significantly down-regulated in gastric cancer tissues and SGC7901 cells by microarray technology were validated by quantitative Real-time PCR （qRT-PCR）. Results： Differential expressions of 26 individual miRNAs between normal samples （including 3 normal gastric tissues and GES-1 cells） and carcinomas （including 24 malignant tissues and SGC7901 cells） were discovered, 19 of them showing down-regulation and 7 up-regulation in carcinoma samples. Hierarchical clustering of the Cancer samples by their miRNA expression accurately separated the carcinomas from normal samples and further their histotypes of carcinomas. The expression levels of miR-433 and miR-9 were significantly down-regulated in gastric cancer tissues and SGC7901cells Conclusion： The differential expression of miR-433 and miR-9 may be used as a novel diagnostic tool for gastric cancer.

关键词： miRNA Real-time PCR Gastric cancer Microarray

P19 of Tomato Bushy Stunt Virus Suppresses RNA Silencing Induced by Short Hairpin RNA in Mammal Cells

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中国病毒学 2007年第3期22卷 199-206页

作者： Wei-xian CHEN　 Juan CHEN　 zhen-zhen zhang　 Ai-long HUANG　 Institute for Viral Hepatitis Key Laboratory on Infectious Disease of Ministry of Education Chongqing University of Medical Science Chongqing 400016 China

To counteract the immune system in parasitic hosts,some viruses encode proteins to suppress the RNA interference(RNAi)*** this report,we established two RNAi systems to be easily observed with strong and obvious *** function of the P19 of tomato bushy stunt virus,which suppresses RNAi in mammal cells,was then studied using these two *** hairpin RNAs targeting green fluorescence protein(pshRNA-GFP)and firefly luciferase(pshRNA-luc)were designed and inserted into a eukaryotic transcriptional vector pTZU6+1,*** shRNA expressing vectors were co-transfected with plasmids containing the target gene with or without *** GFP expression level was assayed by fluorescence microscopy,Western blotting and *** luciferase expression level was analyzed by the dual-luciferase assay *** designed in this study down-regulated the target gene specifically and efficiently,with a decrease of expression of both genes of about 70%,*** P19 was introduced into the RNAi systems,the expression of both GFP and the luciferase were mostly recovered compared with the control *** RNAi systems of GFP and luciferase were constructed successfully,demonstrating that P19 of tomato bushy stunt virus has the ability to counteract the RNAi effect induced by shRNA in mammal cells.

关键词：哺乳动物 RNA干涉番茄丛矮病毒 P19 RNA抑制

Discovery of new characterizations of parameter distributions in a semiempirical mass model:an investigation by dividing an overdetermined system into numerous balanced subsystems

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Chinese Physics C 2022年第10期46卷 184-192页

作者： Hai-Yan Meng Hua-Lei Wang zhen-zhen zhang Min-Liang Liu School of Physics and Microelectronics Zhengzhou UniversityZhengzhou 450001China Key Laboratory of High Precision Nuclear Spectroscopy Institute of Modern PhysicsChinese Academy of SciencesLanzhou 730000China School of Nuclear Science and Technology University of Chinese Academy of SciencesBeijing 100049China

We propose and test a new method of estimating the model parameters of the phenomenological BetheWeizsacker mass *** on the Monte Carlo sampling of a large dataset,we obtain,for the first time,a Cauchy-type parameter distribution formed by the exact solutions of linear equation *** the maximum likelihood estimation,the location and scale parameters are *** estimated results are compared with those obtained by solving overdetermined systems,e.g.,the solutions of the traditional least-squares *** correlations and uncertainty propagation are briefly *** expected,it is also found that improvements in theoretical modeling(e.g.,considering microscopic corrections)decrease the parameter and propagation uncertainties.

关键词： Bethe-Weizsacker mass formula parameter uncertainty potential energy surface calculation maximum likelihood estimation parameter correlation