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Comparative Proteomic Analysis of B. henselae Houston and B. henselae Marseille by Two-dimensional Gel Electrophoresis

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Biomedical and Environmental Sciences 2005年第5期18卷 341-344页

作者： SU-QING ZHAO YAN-FEI CAI zhen-yu zhu Unite des Rickettsies Faculte de MedecineUniversite de la Mediterranee13385 Marseille Cedex 05France Faculty of Chemical Engineering and Light Industry Guangdong University of TechnologyGuangzhou 510090GuangdongChina College of Resource and Environment South China Agriculture UniversityGuangzhou 510642GuangdongChina Department of Biochemistry Zhongshan Medical CollegeSun Yat-Sen UniversityGuangzhou 510080GuangdongChina

To compare the protein difference between B. henselae Houston and B. henselae Marseille by two-dimensional gel electrophoresis. Method Protein samples were prepared by vorterx, ultrasonic treatment, and centrifugation. Protein concentrations were determined by Bradford method. Protein difference was compared by the first IEF and the second SDS-polyacrylamide gel electrophoresis. Results Protein concentrations in samples of Bartonella henselae Houston and Bartonella henselae Marseille were 2.117 μg/μL and 2.200 μg/μL respectively. Sample protein of 40 μg for IPG strips loading was perfect. The results of 2-DE in pH 4 to 7 IPG strips showed that the total protein spots of Bartonella henselae Houston and Bartonella henselae Marseille were 375 and 379 respectively, 95% of the spots were the same between the two strains of Bartonella henselae. Conclusion The procedure of 2-DE may prove successful for the proteomic analysis of Bartonella henselae. Bartonella henselae Houston and Bartonella henselae Marseille are different genotypes.

关键词： Protein B.henselae Two-dimensional gel electrophoresis

Distributed wide field electromagnetic method based on high-order 2^(n) sequence pseudo random signal

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Transactions of Nonferrous Metals Society of China 2022年第5期32卷 1609-1622页

作者： Yang YANG Ji-shan HE Fan LING yu-zhen zhu Geotechnical and Structural Engineering Research Center Shandong UniversityJi’nan 250061China Institute of Urban Underground Space and Energy Studies The Chinese University of HongKongShenzhen 518172China School of Geosciences and Info-Physics Central South UniversityChangsha 410083China Shandong Coal Geological Planning and Exploration Research Institute Ji’nan 250109China

To make three-dimensional electromagnetic exploration achievable,the distributed wide field electromagnetic method(WFEM)based on the high-order 2^(n) sequence pseudo-random signal is proposed and *** this method,only one set of high-order pseudo-random waveforms,which contains all target frequencies,is *** on high-order sequence pseudo-random signal construction algorithm,the waveform can be customized according to different exploration *** the receivers are independent with each other and dynamically adjust the acquisition parameters according to different requirements.A field test in the deep iron ore of Qihe−yucheng showed that the distributed WFEM based on high-order pseudo-random signal realizes the high-efficiency acquisition of massive electromagnetic data in quite a short *** with traditional controlled-source electromagnetic methods,the distributed WFEM is much more *** WFEM can be applied to the large scale and high-resolution exploration for deep resources and minerals.

关键词： distributed wide field electromagnetic method(WFEM) high-order pseudo-random signal multifrequency massive data

Downregulation of EphB2 by RNA interference attenuates glial/fibrotic scar formation and promotes axon growth

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Neural Regeneration Research 2022年第2期17卷 362-369页

作者： Jian Wu zhen-yu zhu Zhi-Wei Fan Ying Chen Ri-yun Yang Yi Li Department of Histology and Embryology Medical CollegeNantong UniversityNantongJiangsu ProvinceChina

The rapid formation of a glial/fibrotic scar is one of the main factors hampering axon growth after spinal cord injury. The bidirectional Eph B2/ephrin-B2 signaling of the fibroblast-astrocyte contact-dependent interaction is a trigger for glial/fibrotic scar formation. In the present study, a new in vitro model was produced by coculture of fibroblasts and astrocytes wounded by scratching to mimic glial/fibrotic scar-like structures using an improved slide system. After treatment with RNAi to downregulate Eph B2, changes in glial/fibrotic scar formation and the growth of VSC4.1 motoneuron axons were examined. Following RNAi treatment, fibroblasts and astrocytes dispersed without forming a glial/fibrotic scar-like structure. Furthermore, the expression levels of neurocan, NG2 and collagen I in the coculture were reduced, and the growth of VSC4.1 motoneuron axons was enhanced. These findings suggest that suppression of Eph B2 expression by RNAi attenuates the formation of a glial/fibrotic scar and promotes axon growth. This study was approved by the Laboratory Animal Ethics Committee of Jiangsu Province, China(approval No. 2019-0506-002) on May 6, 2019.

关键词： astrocyte EphB2 fibroblast glial/fibrotic scar microfluidic platform RNAi spinal cord injury VSC4.1 motoneuron

Mathematical modelling and numerical optimization of particle heating process in copper flash furnace

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Transactions of Nonferrous Metals Society of China 2021年第5期31卷 1506-1517页

作者： Dong-bo GAO Xiao-qi PENG Yan-po SONG zhen-yu zhu Yang DAI School of Energy Science and Engineering Central South UniversityChangsha 410083China Hunan First Normal University Changsha 410205China

A mathematical model of the particle heating process in the reaction shaft of flash smelting furnace was established and the calculation was *** results indicate that radiation plays a significant role in the heat transfer process within the first 0.6 m in the upper part of the reaction shaft,whilst the convection is dominant in the area below 0.6 m for the particle *** order to accelerate the particle ignition,it is necessary to enhance the convection,thus to speed up the particle heating.A high-speed preheated oxygen jet technology was then suggested to replace the nature gas combustion in the flash furnace,aiming to create a lateral disturbance in the gaseous phase around the particles,so as to achieve a slip velocity between the two phases and a high convective heat transfer *** simulation was carried out for the cases with the high-speed oxygen jet and the normal nature gas *** results show that with the high-speed jet technology,particles are heated up more rapidly and ignited much earlier,especially within the area of the radial range of R=0.3−0.6 *** a result,a more efficient smelting process can be achieved under the same operational condition.

关键词： flash smelting process particle heating mathematical model high-speed jet numerical simulation

Experiment and numerical simulation of two-phase flow in oxygen enriched side-blown furnace

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Transactions of Nonferrous Metals Society of China 2020年第1期30卷 249-258页

作者： Yan-ting LIU Tian-zu YANG zhuo CHEN zhen-yu zhu Ling ZHANG Qing HUANG School of Metallurgy and Environment Central South UniversityChangsha 410083China Changsha Design and Research Institute of Nonferrous Metallurgy Changsha 410001China School of Energy Science and Engineering Central South UniversityChangsha 410083China

Taking an oxygen enriched side-blown furnace as the prototype,a hydraulic model was established according to the similarity *** influence of three factors on the gas-liquid two-phase flow was analyzed,*** airflow speed,the submerged depth and the downward angle of the nozzle.A numerical simulation of the hydraulic model was carried out trying to find the suitable turbulence model which can describe the side-blown two-phase flow correctly by comparing the simulation results with the experimental *** experiment shows that the airflow speed has a great influence on the flow of the *** submerged depth of the nozzle has a relatively smaller influence on the penetration depth and the surface fluctuation height in the liquid *** the nozzle is at a downward angle of 15°,the penetration depth and the surface fluctuation height are *** is concluded that the numerical results with the realizable k-εturbulence model are the closest to the experiment for the penetration depth,the surface fluctuation height and the bubble scale.

关键词： side-blown furnace hydraulic model numerical simulation turbulence model

Relation between gravitational mass and baryonic mass for non-rotating and rapidly rotating neutron stars

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Frontiers of physics 2020年第2期15卷 119-128页

作者： He Gao Shun-Ke Ai Zhou-Jian Cao Bing Zhang zhen-yu zhu Ang Li Nai-Bo Zhang Andreas Bauswein Department of Astronomy Beijing Normal UniversityBeijing 100875China Department of Physics and Astronomy University of Nevada Las VegasNV 89154USA Department of Astronomy Xiamen UniversityXiamen 361005China Institute for Theoretical Physics Max-von-Laue-Strasse 160438 FrankfurtGermany Shandong Provincial Key Laboratory of Optical Astronomy and Solar-Terrestrial Environment School of Space Science and PhysicsInstitute of Space SciencesShandong UniversityWeihai 264209China GSI Helmholtzzentrum fiir Schwerionenforschung Planckstrafie 164291 DarmstadtGermany

With a selected sample of neutron star(NS)equations of state(EOSs)that are consistent with the current observations and have a range of maximum masses,we investigate the relations between NS gravitational mass Mg and baryonic mass and the relations between the maximum NS mass supported through uniform rotation(Mmax)and that of nonrotating NSs(Mtov).We find that for an EOS-independent quadratic,universal transformation formula(Mb=Mg+A×M^2/g),the best-fit A value is 0.080 for non-rotating NSs,0.064 for maximally rotating NSs,and 0.073 when NSs with arbitrary rotation are *** residual error of the transformation is?0.1M⊙ for non-spin or maximum-spin,but is as large as?0.2M⊙ for all *** different EOSs,we find that the parameter A for non-rotating NSs is proportional to R^-1/1.4(where R1.4 is NS radius for 1.4M⊙ in units of km).For a particular EOS,if one adopts the best-fit parameters for different spin periods,the residual error of the transformation is smaller,which is of the order of O.O1M⊙ for the quadratic form and less than O.O1M⊙ for the cubic form(Mb=Mg+A1×M^2/g+A2×M^3/g).We also find a very tight and general correlation between the normalized mass gain due to spin △m≡(Mmax-MTOV)MTOV and the spin period normalized to the Keplerian period P,i.e.,log10 △m=(-2.74±0.05)log10 P+log10(0.20±0.01),which is independent of EOS *** empirical relations are helpful to study NS-NS mergers with a long-lived NS merger product using multi-messenger *** application of our results to GW170817 is discussed.

关键词： gravitational waves

Preparation of Artificial Antigen and Egg Yolk-derived Immunoglobulin(IgY) of Citrinin for Enzyme-Linked Immunosorbent Assay

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Biomedical and Environmental Sciences 2009年第3期22卷 237-243页

作者： ZHAO-HUI DUAN zhuANG-SEN LIN HE-RUI YAO YAN-HONG GAO KUN ZHANG Su-QING ZHAO zhen-yu zhu Laboratory of Clinical Immunology the Second Affiliated Hospital of Sun Yat-Sen University Guangzhou 510120 Guangdong China Department of Pharmacy Engineering Faculty of Chemical Engineering and Light Industry Guangdong University of Technology Guangzhou 510006 Guangdong China Guangdong Center for Disease Control and Prevention Guangzhou 510300 Guangdong China Department of Biochemistry Zhongshan Medical College Sun Yat-Sen University Guangzhou 510080 Guangdong China

Objective To prepare artificial antigens and anti-citrinin egg yolk-derived immunoglobulin （IgY） to build an enzyme-linked immunosorbent assay （ELISA） for citrinin （CTN）. Methods CTN was conjugated with bovine serum albumin （BSA）, ovalbumin （OVA） with formaldehyde condensation method to prepare artificial antigens and identified by ultraviolet （UV） spectrometry and Infrared （IR） spectrometry. Artificial antigens for CTN and anti-CTN IgY were purified with polyethylene glycol two-step precipitation method and identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）. ELISA with IgY was established. Cross-reactivity of IgY with various structural similarities to CTN and possible co-occurrence with CTN in agricultural commodities were studied. Results UV and IR absorption spectra suggested that CTN was correlated with the carrier protein of BSA or OVA. SDS-PAGE patterns showed that the anti-CTN IgY was almost pure with a molecular weight of approximate 100 KD. The indirect competitive ELISA showed that the detection limit of CTN was 10 ng·mL^-1, with a good linearity ranging 20-640 ng·mL^-1. Conclusion Artificial antigens of CTN can be successfully synthesized. The established ELISA can be used to determine CTN- contaminated samples.

关键词： Citrinin Artificial antigen lgY ELISA

Treatment of infrarenal abdominal aortic dissection concomitant with an aneurysm

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Chinese Medical Journal 2007年第2期120卷 169-170页

作者： WANG Li-xin FU Wei-guo WANG yu-qi XI Xun GUO Da-qiao CHEN Bin JIANG Jun-hao YANG Ju SHI zhen-yu zhu Ting Department of Vascular Surgery Zhongshan Hospital Fudan University Shanghai 200032 China

Aortic dissection occurs when layers of the aortic ,walls are separated by the blood flow through an intimal tear. Dissection of the aorta most frequently originates in the ascending aorta （70%）, followed by the descending aorta （22%）, the aortic arch （7%） and the abdominal aorta （1/%）.1 The dissection limited to the abdominal aorta is rare.2 An isolated abdominal aortic dissection （IAAD） concomitant with an abdominal aortic aneurysm （AAA） is uncommon. We present here one patient with IAAD and AAA treated by endovascular therapy.

关键词： aneurysm, dissecting aortic aneurysm, abdominal blood vessel prosthesis implantation stent

Two-thiols-regulated fabrication of plasmonic nanoparticles with co-enhanced Raman scattering and circular dichroism responses

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Rare Metals 2023年第11期42卷 3673-3681页

作者： Jia-Qi Chen Han-Bo Li Xi Lu Xiao-Yang Liu Wei Feng Pan Wang Jian-Qiang Zhao zhen-yu zhu Guang-Chao zheng Xiao-Chun Wu School of Mechanical Engineering Chengdu UniversityChengdu610000China CAS Key Laboratory of Standardization and Measurement for Nanotechnology CASNational Center for Nanoscience and TechnologyBeijing100190China University of the Chinese Academy of Sciences Beijing100049China Innovation Research Center of Chiral Drugs Institute for Advanced StudyChengdu UniversityChengdu610106China School of Physics and Microelectronics Key Laboratory of Material PhysicsMinistry of EducationZhengzhou UniversityZhengzhou450001China Institute of Quantum Materials and Physics Henan Academy of SciencesZhengzhou450046China

Plasmonicnanoparticles(PNPs)with stable nanogaps are important to achieve strong,uniform and quantitative gap-enhanced Raman scattering(GERS)*** PNPs with plasmonic circular dichroism(PCD)responses have been discovered to be suitable for applications in enantiomeric recognition,cancer therapy and activation of immune ***,two-thiolsmodulated growth was demonstrated to result in the acquisition of PNPs with synergistically enhanced GERS and PCD signals.4-Aminothiophenol(4-ATP)and cysteine(Cys)played the role of Raman reporter and chiral stimulus,*** a fixed 4-ATP concentration,the GERS signal of PNPs was significantly enhanced with the increase of the concentration of ***,at a fixed concentration of Cys,an increase in PCD response was observed by elevating the concentration of *** aforementioned molecules acted as morphology controllers,leading to the formation of helical *** is suggested that the giant GERS and PCD response were contributed by the‘‘hot spots''within the PNPs and more perfect helical *** research pointed out a novel synthetic guideline to obtain PNPs with multiple functionalities by incorporating multi-ligands into the growth stages.

关键词： Gap-enhanced Raman scattering(GERS) Chirality Thiol Plasmonic nanoparticles