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ANAC005 is a membrane-associated transcription factor and regulates vascular development in Arabidopsis

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Journal of Integrative Plant Biology 2016年第5期58卷 442-451页

作者： Jun Zhao Jiang-Shu Liu fu-Ning Meng zhen-zhen Zhang Hao Long Wen-Hui Lin xiao-Min Luo Zhi-Yong Wang Sheng-Wei Zhu Key Laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China University of Chinese Academy of Sciences Beijing 100049China Institute of Molecular Cell Biology College of Life ScienceHebei Normal UniversityShijiazhuang 050016China School of Life Sciences and Biotechnology Shanghai Jiao Tong UniversityStanfordCalifornia 94305USA Department of Plant Biology Carnegie Institution for ScienceShanghai 200240China

Vascular tissues are very important for providing both mechanical strength and long-distance transport. The molecular mechanisms of regulation of vascular tissue develop- ment are still not fully understood. In this study we identified ANACoo5 as a membrane-associated NAC family transcription factor that regulates vascular tissue development. Reporter gene assays showed that ANACoo5 was expressed mainly in the vascular tissues, increased expression of ANACoo5 protein in transgenic Arabidopsis caused dwarf phenotype, reduced xylem differentiation, decreased lignin content, repression of a lignin biosynthetic gene and genes related to cambium and primary wall, but activation of genes related to the secondary wall. Expression of a dominant repressor fusion of ANACoo5 had overall the opposite effects on vascular tissue differ- entiation and lignin synthetic gene expression. The ANACoo5- GFP fusion protein was localized at the plasma membrane, whereas deletion of the last 20 amino acids, which are mostly basic, caused its nuclear localization. These results indicate that ANACoo5 is a cell membrane-associated transcription factor that inhibits xylem tissue development in Arabidopsis.

关键词： ANACoo5 Arabidopsis NAC vascular tissues development

Poverty Reduction,Environmental Protection and Ecosystem Services:A Prospective Theory for Sustainable Development

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Chinese Geographical Science 2014年第1期24卷 83-92页

作者： zhen Nahui fu Bojie LU Yihe WANG Shuai State Key Laboratory of Urban and Regional Ecology Research Center for Eco-Environmental SciencesChinese Academy of Sciences

Poverty reduction and environmental protection are two global tasks for sustainable *** study perspective has changed over time,from narrowly focusing on poverty reduction to comprehensively strengthening *** reviewed key references targeting the theoretical content and practical approach relying on poverty,environment protection and ecosystem *** discussed the contradicting views on the relationship of poverty and environmental degradation,and then illustrated the study progress of a cutting-age topic-ecosystem services which pave a way to address poverty reduction and environmental protection *** last,we investigated essential factors that affect the development and environmental *** the evolution of the concept of poverty,we found that the environment has occupied an increasing proportion in the cognizance of *** relationship between poverty and environmental degradation is regional *** practical aspect,projects based on the management and valuation assessment of ecosystem services draw researchers′attention all over the *** appropriate scale,essential economic incentives,morality,law and social equality are key factors affecting individuals′decisions which directly relate to the sustainable development.

关键词： poverty environmental degradation ecosystem services sustainable development

λ-Central BMO Estimates for Commutators of Singular Integral Operators with Rough Kernels

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Acta Mathematica Sinica,English Series 2008年第3期24卷 373-386页

作者： Zun Wei fu Yan LIN Shan zhen LU School of Mathematical Sciences Beijing Normal University Beijing 100875 P. R. China Department of Mathematics Linyi Normal Universtiy Linyi 276005 P. R. China

The authors establish λ-central BMO estimates for commutators of singular integral operators with rough kernels on central Morrey spaces. Moreover, the boundedness of a class of multisublinear operators on the product of central Morrey spaces is discussed. As its special cases, the corresponding results of multilinear Calderon-Zygmund operators and multilinear fractional integral operators can be deduced, resDectivelv.

关键词： λ-central BMO space central Morrey space Calderon-Zygmund operator fractionalintegral operator rough kernel

Brazed joints of CBN grains and AISI 1045 steel with AgCuTi-TiC mixed powder as filler materials

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International Journal of Minerals,Metallurgy and Materials 2011年第6期18卷 717-724页

作者： Wen-feng Ding Jiu-hua Xu zhen-zhen Chen Hong-hua Su Yu-can fu College of Mechanical and Electrical Engineering Nanjing University of Aeronautics and Astronautics Nanjing 210016 China

The brazing process of cubic boron nitride （CBN） grains and AISI 1045 steel with AgCuTi-TiC mixed powder as a filler material was carried *** joining strength and the interfacial microstructure were *** experimental results indicate that the spreading of the molten filler material on AISI 1045 steel is decreased with the increase of TiC content.A good interface is formed between the TiC particulates and AgCuTi alloy through the wetting *** the case of AgCuTi＋16wt% TiC,the strength of the brazed steel-to-steel joints reached the highest value of 95MPa dependent upon the reinforcement effect of TiC particles within the filler *** resultants of TiB2,TiB,and TiN are produced at the interface of the CBN grains and the AgCuTi-TiC filler layer by virtue of the interdiffusion of B,N,and Ti atoms.

关键词： grinding wheels cubic boron nitride brazing filler metals shear strength interfaces

Reconstruction of sub cross-correlation cancellation technique for unambiguous acquisition of BOC(kn, n) signals

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Journal of Systems Engineering and Electronics 2019年第5期30卷 852-860页

作者： JI Yuanfa CHEN xiaoqian fu Qiang SUN Xiyan zhen Weimin Guangxi Key Laboratory of Precision Navigation Technology and Application Guilin University of Electronic TechnologyGuilin 541004China National&Local Joint Engineering Research Center of Satellite Navigation Positioning and Location Service Guilin 541004China Key Laboratory of Radio Wave Environment and Modeling Technology Qingdao 266000China

In order to solve the problem of ambiguous acquisition of BOC signals caused by its property of multiple peaks,an unambiguous acquisition algorithm named reconstruction of sub cross-correlation cancellation technique(RSCCT)for BOC(kn,n)signals is *** this paper,the principle of signal decomposition is combined with the traditional acquisition algorithm structure,and then based on the method of reconstructing the correlation *** method firstly gets the sub-pseudorandom noise(PRN)code by decomposing the local PRN code,then uses BOC(kn,n)and the sub-PRN code cross-correlation to get the sub cross-correlation ***,the correlation peak with a single peak is obtained by reconstructing the sub cross-correlation function so that the ambiguities of BOC acquisition are *** simulation shows that RSCCT can completely eliminate the side peaks of BOC(kn,n)group signals while maintaining the narrow correlation of BOC,and its computational complexity is equivalent to sub carrier phase cancellation(SCPC)and autocorrelation side-peak cancellation technique(ASPeCT),and it reduces the computational complexity relative to *** BOC(n,n),the acquisition sensitivity of RSCCT is 3.25 dB,0.81 dB and 0.25 dB higher than binary phase shift keying(BPSK)-like,SCPC and ASPeCT at the acquisition probability of 90%,*** peak to average power ratio is 1.91,3.0 and 3.7 times higher than ASPeCT,SCPC and BPSK-like at SNR=–20 dB,*** BOC(2n,n),the acquisition sensitivity of RSCCT is 5.5 dB,1.25 dB and 2.69 dB higher than BPSK-like,SCPC and ASPeCT at the acquisition probability of 90%,*** peak to average power ratio is 1.02,1.68 and 2.12 times higher than ASPeCT,SCPC and BPSK-like at SNR=–20 dB,respectively.

关键词： signal decomposition sub cross-correlation reconstructingcorrelation function acquisition performance

BnaSD.C3 is a novel major quantitative trait locus affecting semi-dwarf architecture in Brassica napus L.

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Journal of Integrative Agriculture 2023年第10期22卷 2981-2992页

作者： WANG xiao-dong CAI Ying PANG Cheng-ke ZHAO xiao-zhen SHI Rui LIU Hong-fang CHEN Feng ZHANG Wei fu San-xiong HU Mao-long HUA Wei zhenG Ming ZHANG Jie-fu Provincial Key Laboratory of Agrobiology/Institute of Industrial Crops Jiangsu Academy of Agricultural Sciences/Key Laboratory of Cotton and RapeseedMinistry of Agriculture and Rural AffairsNanjing 210014P.R.China Oil Crops Research Institute Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil CropsMinistry of Agriculture and Rural AffairsWuhan 430062P.R.China

Plant height is a key plant architectural trait that affects the seed yield,harvest index and lodging resistance in Brassica napus L.,although the genetic mechanisms affecting plant height remain ***,a semi-dwarf mutant,df34,was obtained by ethyl methanesulphonate-induced *** analysis showed that the semi-dwarf phenotype is controlled by one semi-dominant gene,which was located on chromosome C03 using a bulked segregant analysis coupled with whole-genome sequencing,and this gene was named *** BnaSD.C3 was fine-mapped to a 297.35-kb segment of the“Darmor-bzh”genome,but there was no potential candidate gene for the semi-dwarf trait underlying this ***,the interval was aligned to the Zhongshuang 11 reference ***,combining structural variation analysis,transcriptome sequencing,phytohormone analyses and gene annotation information,BnaC03G0466900ZS and BnaC03G0478900ZS were determined to be the most likely candidate genes affecting the plant height of *** study provides a novel major locus for breeding and new insights into the genetic architecture of plant height in ***.

关键词： Brassica napus L. fine mapping phytohormone analysis plant height transcriptome analysis

Rescattering mechanism of weak decays of double-charm baryons

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Chinese Physics C 2021年第5期45卷 77-99页

作者： Jia-Jie Han Hua-Yu Jiang Wei Liu zhen-Jun xiao fu-Sheng Yu School of Nuclear Science and Technology Lanzhou UniversityLanzhou 730000China Department of Physics and Institute of Theoretical Physics Nanjing Normal UniversityNanjing 210023China Theoretische Physik 1 Naturwissenschaftlich-Technische FakultatUniversitat Siegen57068 SiegenGermany Lanzhou Center for Theoretical Physics Lanzhou UniversityLanzhou 730000China Frontier Science Center for Rare Isotopes Lanzhou UniversityLanzhou 730000China Center for High Energy Physics Peking UniversityBeijing 100871China

The doubly charmed baryon■cc++was recently observed by LHCb via the decay processes of■cc++→∧+cK^(-)π^(+)π^(+)and■c+π^(+).These discovery channels were successfully predicted in a framework in which the short-distance contributions are calculated under the factorization hypothesis and the long-distance contributions are estimated using the rescattering mechanism for the final-state-interaction *** this paper,we illustrate the above framework in detail by systematic studies on the tw o-body baryonic decays Bcc→BcP involving the doubly charmed baryons Bcc=(■cc++,■cc++,Ωcc+),the singly charmed baryons Bc=(B3,B6)and the light pseudoscalar mesons P=(π,K,η1,8).

关键词： double-charm baryon rescattering mechanism final-state interaction

基于微滴式数字PCR检测牛病毒性腹泻病毒方法的建立及初步应用

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农业生物技术学报 2025年第1期33卷 190-199页

作者：刘芯怡李丹秦刚陈俊贞付强史慧君新疆农业大学动物医学学院新疆草食动物新药研究与创制重点实验室天康生物制药有限公司新疆巴楚县畜牧兽医站中国农业科学院西部农业研究中心

牛病毒性腹泻病毒(Bovine viral diarrhea virus, BVDV)是引起犊牛(Bos sp.)病毒性腹泻的主要病原之一，导致犊牛免疫力降低，患病率和死亡率升高。目前对BVDV常用的检测方法存在灵敏度较低、容易出现假阳性等状况。本研究基于微滴式数... 详细信息

牛病毒性腹泻病毒(Bovine viral diarrhea virus, BVDV)是引起犊牛(Bos sp.)病毒性腹泻的主要病原之一，导致犊牛免疫力降低，患病率和死亡率升高。目前对BVDV常用的检测方法存在灵敏度较低、容易出现假阳性等状况。本研究基于微滴式数字PCR(droplet digital PCR, ddPCR)技术，建立BVDV精准检测技术。首先针对BVDV 5'UTR基因保守序列区域设计引物和探针，合成BVDV 5'UTR基因并构建质粒标准品。使用BVDV质粒标准品分别建立实时荧光定量PCR(real-time quantitative PCR, qPCR)和ddPCR检测方法，并优化其反应参数和条件，分析其灵敏度、特异性和重复性，并进行了临床应用。结果显示，本研究建立的qPCR方法中，最佳引物和探针浓度分别为300和500 nmol/L，标准曲线呈良好的线性关系，最低检测下限为1×10～4 copies/μL，具有良好的特异性和重复性。建立的ddPCR方法中，最低检测下限为0.64 copies/μL，比qPCR灵敏性高15 625倍，该方法具有良好的特异性和重复性。临床检测了82份样品，ddPCR检出率为15.58%,qPCR检出率为14.63%。本研究建立的ddPCR方法比qPCR方法更加灵敏、高效、特异且重复性良好，为BVDV鉴别诊断提供了技术支持。

关键词：牛病毒性腹泻病毒(BVDV) 微滴式数字PCR(ddPCR) 实时荧光定量PCR(qPCR) 灵敏性特异性重复性

Research on design and firing performance of Si-based detonator

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Defence Technology（防务技术） 2014年第1期10卷 34-39页

作者： Rui-zhen XIE xiao-ming REN Lan LIU Yan XUE Dong-xiao fu Rui ZHANG Science and Technology on Applied Physical Chemistry Laboratory Shaanxi Applied Physics-Chemistry Research Institute

For the chip integration of MEMS(micro-electromechanical system) safety and arming device, a miniature detonator needs to be developed to reduce the weight and volume of explosive train. A Si-based micro-detonator is designed and fabricated, which meets the requirement of MEMS safety and arming device. The firing sensitivity of micro-detonator is tested according to GJB/z377A-94 sensitivity test methods:Langlie. The function time of micro-detonator is measured using wire probe and photoelectric transducer. The result shows the average firing voltage is 6.4 V when the discharge capacitance of firing electro-circuit is 33 mF. And the average function time is 5.48 ms. The firing energy actually utilized by Si-based micro-detonator is explored.

关键词： Si基雷管设计解除保险装置微机电系统性能烧制测试方法