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Chondroitinase ABC combined with Schwann cell transplantation enhances restoration of neural connection and functional recovery following acute and chronic spinal cord injury

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Neural Regeneration Research 2025年第5期20卷 1467-1482页

作者： Wenrui Qu xiangbing Wu Wei Wu Ying Wang Yan Sun Lingxiao Deng Melissa Walker chen chen Heqiao Dai Qi Han Ying Ding Yongzhi xia George Smith Rui Li Nai-Kui Liu xiao-Ming Xu Spinal Cord and Brain Injury Research Group Stark Neurosciences Research InstituteDepartment of Neurological SurgeryIndiana University School of MedicineIndianapolisINUSA Department of Hand Surgery the Second Hospital of Jilin UniversityChangchunJilin ProvinceChina Shriners Hospitals Pediatric Research Center Temple University School of MedicinePhiladelphiaPAUSA Department of Anatomy&Cell Biology Indiana University School of MedicineIndianapolisINUSA

Schwann cell transplantation is considered one of the most promising cell-based therapy to repair injured spinal cord due to its unique growth-promoting and myelin-forming properties.A the Food and Drug Administration-approved Phase I clinical trial has been conducted to evaluate the safety of transplanted human autologous Schwann cells to treat patients with spinal cord injury.A major challenge for Schwann cell transplantation is that grafted Schwann cells are confined within the lesion cavity,and they do not migrate into the host environment due to the inhibitory barrier formed by injury-induced glial scar,thus limiting axonal reentry into the host spinal *** we introduce a combinatorial strategy by suppressing the inhibitory extracellular environment with injection of lentivirus-mediated transfection of chondroitinase ABC gene at the rostral and caudal borders of the lesion site and simultaneously leveraging the repair capacity of transplanted Schwann cells in adult rats following a mid-thoracic contusive spinal cord *** report that when the glial scar was degraded by chondroitinase ABC at the rostral and caudal lesion borders,Schwann cells migrated for considerable distances in both rostral and caudal *** Schwann cell migration led to enhanced axonal regrowth,including the serotonergic and dopaminergic axons originating from supraspinal regions,and promoted recovery of locomotor and urinary bladder ***,the Schwann cell survival and axonal regrowth persisted up to 6 months after the injury,even when treatment was delayed for 3 months to mimic chronic spinal cord *** findings collectively show promising evidence for a combinatorial strategy with chondroitinase ABC and Schwann cells in promoting remodeling and recovery of function following spinal cord injury.

关键词： axonal regrowth bladder function chondroitinase ABC functional recovery glial scar lentivirus migration Schwann cell spinal cord injury transplantation

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3D Printing of Tough Hydrogel Scaffolds with Functional Surface Structures for Tissue Regeneration

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Nano-Micro Letters 2025年第2期17卷 18-45页

作者： Ke Yao Gaoying Hong Ximin Yuan Weicheng Kong Pengcheng xia Yuanrong Li Yuewei chen Nian Liu Jing He Jue Shi Zihe Hu Yanyan Zhou Zhijian Xie Yong He State Key Laboratory of Fluid Power and Mechatronic Systems School of Mechanical EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China Liangzhu Laboratory Zhejiang UniversityHangzhou 310027People’s Republic of China Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province College of Mechanical EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China Stomatology Hospital School of StomatologyZhejiang University School of MedicineHangzhou 310027People’s Republic of China State Key Laboratory of Advanced Welding and Joining Harbin Institute of TechnologyHarbin 150001People’s Republic of China Institute of Digital Medicine Nanjing First HospitalNanjing Medical UniversityNanjing 210006People’s Republic of China

Hydrogel scaffolds have numerous potential applications in the tissue engineering ***,tough hydrogel scaffolds implanted in vivo are seldom reported because it is difficult to balance biocompatibility and high mechanical *** by Chinese ramen,we propose a universal fabricating method(printing-P,training-T,cross-linking-C,PTC&PCT)for tough hydrogel scaffolds to fill this ***,3D printing fabricates a hydrogel scaffold with desired structures(P).Then,the scaffold could have extraordinarily high mechanical properties and functional surface structure by cycle mechanical training with salting-out assistance(T).Finally,the training results are fixed by photo-cross-linking processing(C).The tough gelatin hydrogel scaffolds exhibit excellent tensile strength of 6.66 MPa(622-fold untreated)and have excellent ***,this scaffold possesses functional surface structures from nanometer to micron to millimeter,which can efficiently induce directional cell ***,this strategy can produce bionic human tissue with mechanical properties of 10 kPa-10 MPa by changing the type of salt,and many hydrogels,such as gelatin and silk,could be improved with PTC or PCT *** experiments show that this scaffold can effectively promote the new generation of muscle fibers,blood vessels,and nerves within 4 weeks,prompting the rapid regeneration of large-volume muscle loss injuries.

关键词： 3D printing Tough hydrogel scaffold Functional surface structure Tissue regeneration Biomaterials

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Stable copper boron–imidazolate cage as Cu-riched enzyme for highly efficient electrocatalytic CO2 reduction to ethylene

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Science China Chemistry 2025年

作者： Jun-Qiang chen Qiao-Hong Li Qin-Long Hong Ping Shao Hai-xia Zhang Jian Zhang State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences

Single-atom catalyst has garnered widespread attention to mimic mature enzymes due to its well-defined atomic structure and coordination environments. However, since the carbon–carbon（C–C） coupling reactions require synergistic catalysis of multiple sites, single-atom catalysts suffer from insufficient active sites and unclear reaction mechanisms. Controlling the reaction intermediates in a precisely targeted pocket through careful metal–organic cage design is therefore crucial. Here, we prepare a tetrahedral [Cu6L4]-type boron–imidazolate cage integrating highly active Cu sites and optimized cavity, which exhibits enzyme like specific catalytic performance in electrochemical CO2reduction reaction（CO2RR） to enhance the selectivity of *** analyses and computational calculations suggest that the single Cu site together with neighboring boron–imidazolate ligands provides suitably synergistic effects that enable the energetically favorable formation of an *COCHO intermediate, a key step determining selectivity. As a result, the [Cu6L4]-type cage of BIC-145 achieves a Faradaic efficiency of 28% for C2H4maintaining an average current density of -3.54 mA cm-2over a 5-hour electrolysis period. This work represents the first example for studying single-metal site catalysts with ultra-low coordination numbers through the rational design of metal–organic cages.

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Insufficient MIRO1 contributes to declined oocyte quality during reproductive aging

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Science China Life Sciences 2025年

作者： zhen-Nan Pan Hao-Lin Zhang Kun-Huan Zhang Jia-Qian Ju Jing-Cai Liu Shao-chen Sun College of Animal Science and Technology Nanjing Agricultural University Reproductive Medicine Centre The Affiliated Yantai Yuhuangding Hospital of Qingdao University

Mitochondrial Rho-GTPase 1 (MIRO1) is an outer mitochondrial membrane protein which regulates mitochondrial transport and mitophagy in mitosis. In present study, we reported the crucial roles of MIRO1 in mammalian oocyte meiosis and its potential relationship with aging. We found that MIRO1 expressed in mouse and porcine oocytes, and its expression decreased in aged mice. MIRO1 deficiency caused the failure of meiotic resumption and polar body extrusion in both mouse and porcine oocytes, which could be rescued by exogenous MIRO1supplementation. Mass spectrometry data indicated that MIRO1 associated with several cytoskeleton and cell cycle-related proteins, and MIRO1 regulated motor protein Dynein for microtubule-organizing centers (MTOCs) dynamics at germinal vesicle (GV) stage, which determined meiotic resumption. Furthermore, we found that MIRO1 regulated Aurora A and kinesin family member 11 (KIF11) for meiotic spindle assembly in oocytes. Besides, MIRO1 associated with several mitochondria-related proteins dynamic-related protein 1 (DRP1),Parkin and lysosomal-associated membrane protein 2 (LAMP2) for mitochondrial dynamics and mitophagy during oocyte meiosis. Taken together, our results suggested that MIRO1 played pivotal roles in meiotic resumption, spindle assembly and mitochondrial function in mouse and porcine oocytes, and its insufficiency might contribute to the oocyte maturation defects during aging.

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Dissecting the cell microenvironment of ovarian endometrioma through single-cell RNA sequencing

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Science China Life Sciences 2025年第1期 116-129页

作者： Jiangpeng Wu Siyu xia Wenting Ye Yan Sun Jing Cai Fubing Yu Haiping Wen Xiuwei Yi Taikang Li Mingwei chen Jiayun chen Ge Song Chuanbin Yang Yali Song Jigang Wang Department of Reproductive Medicine Dongguan Maternal and Child Health Care Hospital Department of Urology and Shenzhen Clinical Research Centre for GeriatricsShenzhen People's HospitalSouthern University of Science and Technology State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs Artemisinin Research Centerand Institute of Chinese Materia MedicaChina Academy of Chinese Medical Sciences Reproductive Medicine Center Foshan Women and Children Hospital State Key Laboratory of Antiviral Drugs School of PharmacyHenan University

Ovarian endometrioma（OE）, also known as “chocolate cysts,” is a cystic mass that develops in the ovaries due to endometriosis and is a common gynecological condition characterized by the growth of endometrial tissue outside the uterus, leading to symptoms such as dysmenorrhea, pelvic pain, and infertility. However, the precise molecular and cellular mechanisms driving this pathophysiology remain largely unknown, posing challenges for diagnosis and treatment. Here, we employed integrated single-cell transcriptomic profiling of over 52,000 individual cells from endometrial tissues of OE patients and healthy donors and identified twelve major cell populations. We identified notable alterations in cell type-specific proportions and molecular signatures associated with OE. Notably, the activation of IGFBP5+macrophages with pro-inflammatory properties, NK cell exhaustion, and aberrant proliferation of IQCG+and KLF2+epithelium are key features and may be the potential mechanisms underlying the pathogenesis of OE. Collectively, our data contribute to a better understanding of OE at the single cell level and may pave the way for the development of novel therapeutic strategies.

关键词： ovary high-throughput sequencing transcriptomics gene expression analysis ligand-receptor analysis

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Palladium installed copper-organic framework for C–C coupling reactions

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Science China Chemistry 2025年

作者： Yu-Mei Wang Mei-xia Tao Kai-Ming Mo Xu chen Shu-Hua Zhong Elena S Shubina Guo-Hong Ning Dan Li College of Chemistry and Materials Science Guangdong Provincial Key Laboratory of Supramolecular Coordination ChemistryJinan University A.N.Nesmeyanov Institute of Organoelement Compounds Russian Academy of Sciences

The formation of C–C bonds is a crucial aspect of organic synthesis. Synthesists' long-term objective is to identify novel catalysts to optimize reaction systems based on traditional transition metal catalysis. This study explores the synthesis and catalytic potential of palladium-installed covalent metal-organic frameworks(CMOFs) based on copper cyclic trinuclear units(Cu-CTUs). By incorporating palladium ions into the imine-based framework JNM-7 through a straightforward immersion method, we achieved a notable enhancement in catalytic activity for critical C–C coupling reactions, including C–H arylation and Sonogashira cross-coupling. The resulting JNM-7-Pd demonstrated remarkable stability and recyclability, maintaining its efficiency over at least three cycles without any decline in efficiency.

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The Dynamic Analyses of Coastal Land Use Patterns in Inner Lingdingyang,the Pearl River Estuary

The Dynamic Analyses of Coastal Land Use Patterns in Inner L...

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海洋地质、矿产资源与环境学术研讨会

作者： xia zhen chen Yunzhen LEI Yong JIA Peihong ( The Key Laboratory of Coast & Island Development of Ministry of Education, Nanjing University, Nanjing 210093, P. R. China Guangzhou Marine Geological Survey, Guangzhou, 510760 Jiangsu Provincial Survey Bureau, Nanjing, 210013, P. R. China )

To satisfy the growing land demand from economic development, a large scale of land reclamation has been carried out in inner Lingdingyang area in recent years. As a result, the tidal flat and the water channels are narrowed, the frequency of flooding increased and the environment undermined. Guangzhou Marine Geological Survey (GMGS) conducted an integrated project for marine geo-environment & geo-hazards survey in 2003. With the integration of multi-temporal remote sensing (using the images of 1977, 1978, 1988, 1996, and 2003), GIS spatial analyzing approach and GPS technique, as well as field data and other background data of the region, this research investigates the comprehensive characteristics and the drivers of coastal land use dynamics and shoreline changes in inner Lingdingyang area. It is revealed that the reclaimed coastal land was mainly for agriculture and aquaculture in early years, but now they have been converted to construction sites of harbors and industries, especially high-tech industry.

关键词： Inner Lingdingyang, Coastal Land Use pattern, Dynamic Analysis

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Community attitudes toward ecotourism development and environmental conservation in nature reserve:a case of Fujian Wuyishan National Nature Reserve,China

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Journal of Mountain Science 2017年第7期14卷 1405-1418页

作者： chen Bi-xia QIU zhen-mian Faculty of Agriculture University of the RyukyusNishihara Town School of Economics Fujian Normal University Kanazawa University

Using social exchange theory, this study investigated residents＇ attitudes toward the preliminary stage of tourism development in nature reserves in Wuyishan National Nature Reserve of China, and the socio-economic and negative effects of residents＇ attitude toward the tourist industry. Results of the questionnaire survey indicated that local people perceived they could benefit from the economic activities related to tourism, and were supportive of the conservation of natural resources and local culture, sustainable community development, and community participation in ecotourism planning and management. This study also revealed that the variables of age, gender, education level, household income, family size, non-farm work arrangements, and the distance to tourism attractions, have significant association with respondents＇ attitudes toward ecotourism development, or negative impacts of the tourist industry. Respondents who are male, or have a higher household income, are more supportive of tourism development inside the reserve. Younger and more highly educated community members are more likely to support learning more about natural and cultural resources and landscapes. Respondents who have a hieher household income, or live far fromthe village center, are more concerned about the negative environmental impacts of tourism development.

关键词： Economic benefits Environmentalconservation Nature reserve Residents' attitude Tourism development Wuyishan National NatureReserve

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L log L criterion for a class of multitype superdiffusions with non-local branching mechanisms

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Science China Mathematics 2019年第8期62卷 1439-1462页

作者： zhen-Qing chen Yan-xia Ren Renming Song Department of Mathematics University of Washington Seattle WA 98195 USA LMAM School of Mathematical Sciences & Center for Statistical SciencePeking University Beijing 100871 China Department of Mathematics University of Illinois Urbana IL 61801 USA

In this paper, we provide a pathwise spine decomposition for multitype superdiffusions with nonlocal branching mechanisms under a martingale change of measure. As an application of this decomposition,we obtain a necessary and sufficient condition(called the L log L criterion) for the limit of the fundamental martingale to be non-degenerate. This result complements the related results obtained in Kyprianou et al.(2012),Kyprianou and Murillo-Salas(2013) and Liu et al.(2009) for superprocesses with purely local branching mechanisms and in Kyprianou and Palau(2018) for super Markov chains.

关键词： multitype superdiffusion non-local branching mechanism switched diffusion spine decomposition martingale

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Electro-generation of TGA by Ni-mSA-mCS bipolar membrane

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Chinese Chemical Letters 2007年第11期18卷 1411-1415页

作者： zhen xia Huang Xi zheng Ri Yao chen xiao chen zhen chen Experiment Center College of Chemistry and Materials Science Fujian Normal University Fuzhou 350007 China

Ni-mSA-mCS bipolar membrane （BM） was prepared by sodium alginate （SA） and chitosan （CS）, which were modified by Ca^2＋ and glutaraldehyde as linking reagents, respectively, mSA-mCS membrane was characterized by FTIR, SEM, TG and used as a separator in the electrolysis cell to produce thioglycolic acid （TGA）. The experiment results show that TGAwas prepared effectively by electro-reduction of dithiodiglycolic acid （DTDGA） with the mixture of TGA and DTDGA in the cathodic chamber. The current efficiency was up to 66.7% at the room temperature （25 ℃） during the current density of 10 mA/cm^2. Compared with the traditional metal reduction method, the electro-reduction technology saves the zinc powder and eliminates the pollution to environment.

关键词： Bipolar membrane Modified sodium alginate Modified chitosan Thioglycolic acid Dithiodiglycolic acid

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