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Decreased expression of HDAC8 indicates poor prognosis in patients with intrahepatic cholangiocarcinoma

Hepatobiliary & Pancreatic Diseases International 2019年第5期18卷 464-470页

作者： Yong-Jie Zhu Qing Xu Ming-Yang Shao Xiao-Yue Cao zhen-ru wu Yu-Wei Chen Hong Bu Yu-Jun Shi Laboratory of Pathology West China HospitalSichuan University37 Guoxue RoadChengdu 610041China Key Laboratory of Transplant Engineering and Immunology NHCWest China HospitalSichuan UniversityChengdu 610041China Department of Pathology West China HospitalSichuan UniversityChengdu 610041China

Background: Intrahepatic cholangiocarcinoma (ICC) is a highly malignant primary tumor in the liver, and the rates of incidence and mortality are rapidly increasing globally. Histone deacetylase 8 (HDAC8) is a transcriptional regulator and is associated with tumorigenesis of several tumor types. This study aimed to evaluate the correlation between HDAC8 expression and clinicopathological parameters in ICC patients. Methods: ICC tissues and corresponding nonmalignant bile duct tissues were obtained from 60 patients. HDAC8 and Ki-67 expression were evaluated by immunohistochemistry staining. HDAC8 expression and the clinicopathological features and prognosis of the patients were analyzed. The mRNA level of HDAC8 in ICC was further analyzed using data from The Cancer Genome Atlas (TCGA). Results: The expression of HDAC8 were lower in ICC tissues (39/60, 65%) than in the corresponding nonmalignant bile duct tissues (54/60, 90%)( P = 0.001). Low HDAC8 expression in ICC was significantly associated with lymph node metastases (47.6% vs. 17.9%, P = 0.015). In addition, the positive cells rate of HDAC8 was statistically and negatively correlated with the Ki-67 index in ICC lesions ( r =?0.7660, P < 0.001). Importantly, the overall survival rate and recurrence-free survival rate in ICC patients with low HDAC8 expression were lower than those with high HDAC8 expression ( P = 0.008 and P = 0.011, respectively). Conclusions: Decreased HDAC8 expression in ICC is related to poor prognosis, and HDAC8 may be an independent prognostic indicator of ICC patients after curative resection.

关键词： Intrahepatic cholangiocarcinoma HDAC8 Prognosis

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Elevated expression of Gsα in intrahepatic cholangiocarcinoma associates with poor prognosis

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Hepatobiliary & Pancreatic Diseases International 2017年第6期16卷 638-644页

作者： Xiao-Yue Cao Hong-Jie Ji Yong-Jie Zhou Xu-Feng Lu Jun-Yi Shen zhen-ru wu Hong Bu Yu-Jun Shi Laboratory of Pathology West China Hospital Sichuan University Chengdu 610041 China Key Laboratory of Transplant Engineering and Immunology NHFPCWest China Hospital Sichuan University Chengdu 610041 China Department of Liver Surgery & Liver Transplantation Center West China Hospital Sichuan University Chengdu 610041 China Department of Pathology West China Hospital Sichuan University Chengdu 610041 China

BACKGROUND:The stimulatory G protein α subunit(Gsα)plays important roles in diverse cell processes including tu morigenesis. Activating mutations in Gsα gene(GNAS) have been reported to be associated with poor prognosis in various human carcinomas. Furthermore, Gsα signaling is crucial in promoting liver regeneration by interacting with growth factor signaling, indicating that Gsα might play a promoting role in cancer development. However, little is known about the correlation between Gsα levels and clinicopathological pa rameters in intrahepatic cholangiocarcinoma(ICC). METHODS:We performed immunoblotting to examine the expression levels of Gsα and Ki67 proteins in tumor tissues and the corresponding adjacent tissues. A total of 74 pair of specimens resected from 74 ICC patients were examined. The association between Gsα levels and clinicopathological find ings and prognosis of the patients was evaluated.RESULTS:Western blotting demonstrated that the expression of Gsα was significantly higher in ICC tissues compared with that in their corresponding adjacent tissues. Gsα protein was highly expressed in about half of ICC tissues(48.6%, 36/74)while only 28.4%(21/74) of tumor adjacent tissues showed Gsα high expression(P=0.011). High Gsα expression in ICC was significantly associated with the numbers of tumor nodules(P=0.037) and lymph node metastases(P=0.010)Moreover, the level of Gsα was significantly and positivelycorrelated with Ki67 expression(P<0.001). In addition, the recurrence-free survival rate and overall survival rate in the Gsα high group were significantly lower than those in the Gsα low group(P=0.004 and P=0.005, respectively).CONCLUSIONS:High Gsα expression is correlated with poor prognosis in ICC patients. Gsα might serve as a potential prognostic indicator of ICC.

关键词： Gsα intrahepatic cholangiocarcinoma immunohistochemistry prognosis

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Standard liver weight model in adult deceased donors with fatty liver: A prospective cohort study

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World Journal of Gastroenterology 2021年第39期27卷 6701-6714页

作者： Bo Li Pan-Yu Chen Yi-Fei Tan He Huang Min Jiang zhen-ru wu Chen-Hao Jiang Dao-Feng zheng Diao He Yu-Jun Shi Yan Luo Jia-Yin Yang Department of Liver Surgery Liver Transplantation CentreWest China Hospital of Sichuan UniversityChengdu 610041Sichuan ProvinceChina Laboratory of Liver Transplantation Frontiers Science Center for Disease-related Molecular NetworkWest China Hospital of Sichuan UniversityChengdu 610041Sichuan ProvinceChina Operating Room West China HospitalSichuan University/West China School of NursingSichuan UniversityChengdu 610041Sichuan ProvinceChina Department of Medical Ultrasound West China Hospital of Sichuan UniversityChengdu 610041Sichuan ProvinceChina Department of Epidemiology and Health Statistics West China School of Public Health and West China Fourth HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina Laboratory of Pathology Key Laboratory of Transplant Immunology and EngineeringNational Health CommissionWest China Hospital of Sichuan UniversityChengdu 610041Sichuan ProvinceChina

BACKGROUND Standard liver weight(SLW)is frequently used in deceased donor liver transplantation to avoid size mismatches with the recipient.However,some deceased donors(DDs)have fatty liver(FL).A few studies have reported that FL could impact liver size.To the best of our knowledge,there are no relevant SLW models for predicting liver size.AIM To demonstrate the relationship between FL and total liver weight(TLW)in detail and present a related SLW formula.METHODS We prospectively enrolled 212 adult DDs from West China Hospital of Sichuan University from June 2019 to February 2021,recorded their basic information,such as sex,age,body height(BH)and body weight(BW),and performed abdominal ultrasound(US)and pathological biopsy(PB).The chi-square test and kappa consistency score were used to assess the consistency in terms of FL diagnosed by US relative to PB.Simple linear regression analysis was used to explore the variables related to TLW.Multiple linear regression analysis was used to formulate SLW models,and the root mean standard error and interclass correlation coefficient were used to test the fitting efficiency and accuracy of the model,respectively.Furthermore,the optimal formula was compared with previous formulas.RESULTS Approximately 28.8%of DDs had FL.US had a high diagnostic ability(sensitivity and specificity were 86.2%and 92.9%,respectively;kappa value was 0.70,P<0.001)for livers with more than a 5%fatty change.Simple linear regression analysis showed that sex(R2,0.226;P<0.001),BH(R2,0.241;P<0.001),BW(R2,0.441;P<0.001),BMI(R2,0.224;P<0.001),BSA(R2,0.454;P<0.001)and FL(R2,0.130;P<0.001)significantly impacted TLW.In addition,multiple linear regression analysis showed that there was no significant difference in liver weight between the DDs with no steatosis and those with steatosis within 5%.Furthermore,in the context of hepatic steatosis,TLW increased positively(nonlinear);compared with the TLW of the non-FL group,the TLW of the groups with hepatic steatosis within 5%,between 5%and 20%and more than 20%in

关键词： Standard liver weight Body surface area Fatty liver Sex Deceased donors

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Effectiveness of additional resection of the invasive cancer-positive proximal bile duct margin in cases of hilar cholangiocarcinoma

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Hepatobiliary Surgery and Nutrition 2018年第4期7卷 251-269页

作者： Wen-Jie Ma zhen-ru wu Anuj Shrestha Qin Yang Hai-Jie Hu Jun-Ke Wang Fei Liu Rong-Xing Zhou Quan-Sheng Li Fu-Yu Li Department of Biliary Surgery West China HospitalSichuan UniversityChengdu 610041China Laboratory of Pathology West China HospitalSichuan UniversityChengdu 610041China Department of General Surgery Andaki Medical CollegePokharaNepal

Background: The survival benefits of additional resection of the positive proximal ductal margin (PM) in hilar cholangiocarcinoma (HCCA) remains controversial. This retrospective study investigated the effectiveness of additional resection of the invasive cancer PM under different levels of preoperative carbohydrate antigen 19-9 (CA19-9). Methods: Patients who underwent hepatectomy for HCCA from 2000 to 2017 were analyzed. Surgical variables, resection margin status, length of the PM (LPM), prognostic factors, and survival were evaluated. Results: A total of 228 patients were enrolled: 175 PM(?) without additional resection patients (group A), 21 PM(?) after additional resection (group B), 16 PM(+) without additional resection (group C), and 16 PM(+) after additional resection (group D). The median survival of group B (20.99 months) was similar to that of group A (23.00 months;P=0.16), and both were significantly better than those of group C (11.60 months) and D (9.50 months), especially when preoperative CA19-9>150 U/mL (P10 mm was significantly better compared with those with an LPM ≤10 mm, especially when preoperative CA19-9 was >150 U/mL (P10 mm group, the survival of group B was comparable with that of group A (P>0.05). Conclusions: HCCA patients could get a survival benefit from a negative PM resulting from additional resection. Survival could be comparable with that of negative PM without additional resection among HCCA patients. An LPM >10 mm is possibly more associated with better survival compared with whether additional resection of the positive PM is performed under different levels of preoperative CA19-9.

关键词： Klatskin tumor margins of excision bile ducts antigens neoplasm carbohydrate antigen 19-9 (CA19-9)

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联合肝外胆管切除应用于进展期胆囊癌是否合理?

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Gastroenterology Report 2019年第6期7卷 426-433,I0002页

作者： Jun-Ke Wang Wen-Jie Ma zhen-ru wu Qin Yang Hai-Jie Hu Fei Liu Fu-Yu Li Department of Biliary Surgery West China HospitalSichuan UniversityChengduSichuanP.R.China Laboratory of Pathology West China HospitalSichuan UniversityChengduSichuanP.R.China

背景:胆囊癌手术中,肝外胆管是否应常规切除仍然存在争议。本研究旨在探讨联合肝外胆管应用于进展期胆管癌根治性手术的临床意义。方法:213例行根治性手术的T2-T4期胆囊癌患者纳入研究。比较术中联合与未联合胆外胆管切除患者的临床病... 详细信息

背景:胆囊癌手术中,肝外胆管是否应常规切除仍然存在争议。本研究旨在探讨联合肝外胆管应用于进展期胆管癌根治性手术的临床意义。方法:213例行根治性手术的T2-T4期胆囊癌患者纳入研究。比较术中联合与未联合胆外胆管切除患者的临床病理特征。同时,采用单因素和多因素Cox比例风险回归模型来明确影响总体生存(OS)的危险因素。结果:213例入组患者中,87例(40.8%)行联合肝外胆管切除。与未行肝外胆管切除者相比,联合肝外胆管切除者术后并发症发生率更高(33.3%vs 21.4%,P=0.046)。然而,联合肝外胆管切除者中位OS显著延长(25 vs 11个月,P=0.008)。按T分期和N分期进行的亚组分析显示,在T3、T4或有淋巴结转移病例中,联合肝外胆管切除者中位OS均优于未行肝外胆管切除者(T3:15 vs 7个月,P=0.002;T4:11 vs 6个月,P=0.021;N+:12 vs 7个月,P<0.001);而在T2或无淋巴结转移病例中,联合肝外胆管切除则未显示出生存获益。多因素预后危险因素分析显示,T分期、淋巴结转移、切缘状态、术前CA19-9水平及肝外胆管切除是进展期胆囊癌患者总体生存的独立预后因素(均P<0.05)。结论:联合肝外胆管切除可独立影响进展期胆囊癌患者的总体生存时间。对于T3、T4或有淋巴结转移的胆囊癌,联合肝外胆管切除是合理的,可改善患者总体生存。

关键词：肝外胆管切除 Cox比例风险回归模型进展期胆囊癌根治性手术胆管癌亚组分析无淋巴结转移 T分期

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Advances of Electrochemical and Electrochemiluminescent Sensors Based on Covalent Organic Frameworks

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Nano-Micro Letters 2024年第2期16卷 395-422页

作者： Yue Cao ru wu Yan‑Yan Gao Yang Zhou Jun‑Jie Zhu Key Laboratory for Organic Electronics and Information Displays(KLOEID)and Institute of Advanced Materials(IAM) Nanjing University of Posts and Telecommunications(NJUPT)Nanjing 210023People’s Republic of China State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023People’s Republic of China

Covalent organic frameworks(COFs),a rapidly developing category of crystalline conjugated organic polymers,possess highly ordered structures,large specific surface areas,stable chemical properties,and tunable pore microenvironments.Since the first report of boroxine/boronate ester-linked COFs in 2005,COFs have rapidly gained popularity,showing important application prospects in various fields,such as sensing,catalysis,separation,and energy storage.Among them,COFs-based electrochemical(EC)sensors with upgraded analytical performance are arousing extensive interest.In this review,therefore,we summarize the basic properties and the general synthesis methods of COFs used in the field of electroanalytical chemistry,with special emphasis on their usages in the fabrication of chemical sensors,ions sensors,immunosensors,and aptasensors.Notably,the emerged COFs in the electrochemiluminescence(ECL)realm are thoroughly covered along with their preliminary applications.Additionally,final conclusions on state-of-the-art COFs are provided in terms of EC and ECL sensors,as well as challenges and prospects for extending and improving the research and applications of COFs in electroanalytical chemistry.

关键词： Covalent organic frameworks Electrochemistry Electrochemiluminescence Sensors

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Super-Resolution Fluorescence Microscopy for Cilia Investigation and Ciliopathy Diagnosis(Invited)

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激光与光电子学进展 2024年第6期61卷 260-271页

作者： Liu zhen wu Yang Division of Life Science The Hong Kong University of Science and TechnologyHong KongChina

The last two decades have witnessed the invention and development of super-resolution microscopy(SRM)that breaks the diffraction limit of light and pushes the fluorescence microscopy resolution to several nanometers.While SRM is widely used in biological studies,such as resolving subdiffraction structures,molecular mapping,tracking single molecules,probing protein-protein interactions,and observing organelle dynamics,its direct application in translational medicine,such as disease diagnosis,is still preliminary.Despite their small size,cilia play a crucial role as organelles in cell signaling and motility,with defects in cilia leading to ciliopathy.Similar to other miniature organelles and macromolecular complexes,cilia are ideal for super-resolution imaging.In this review,we will 1)introduce cilia and ciliopathy,2)show how SRM extends our knowledge of cilia,and 3)focus on how SRM improves the diagnosis of motile ciliopathies.

关键词： diagnosis limit spite

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An interlaminar damage shell model for typical composite structures

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Chinese Journal of Aeronautics 2024年第1期37卷 118-137页

作者： Jie ZHOU zhen wu zhengliang LIU Xiaohui REN School of Aeronautics Northwestern Polytechnical UniversityXi’an 710072China National Key Laboratory of Strength and Structural Integrity Xi’an 710065China School of Mechanical Engineering Xi’an Aeronautical UniversityXi’an 710065China

Using the plate/shell elements in commercial software,accurate analysis of interlaminar initial damage in typical composite structures is still a challenging issue.To propose an accurate and efficient model for analysis of interlaminar initial damage,the following work is carried out:(A)A higher-order theory is firstly proposed by introducing the local Legendre polynomials,and then a novel shell element containing initial damage prediction is developed,which can directly predict transverse shear stresses without any postprocessing methods.Unknown variables at each node are independent of number of layers,so the proposed model is more efficient than the 3D-FEM.(B)Compression experiment is carried out to verify the capability of the proposed model.The results obtained from the proposed model are in good agreement with experimental data.(C)Several examples have been analyzed to further assess the capability of the proposed model by comparing to the 3D-FEM results.Moreover,accuracy and efficiency have been evaluated in different damage criterion by comparing with the selected models.The numerical results show that the proposed model can well predict the initial interlaminar damage as well as other damage.Finally,the model is implemented with UEL subroutine,so that the present approach can be readily utilized to analyze the initial damage in typical composite structures.

关键词： Compression experiment Finite element method Initial damage Interlaminar stress Plate/shell element

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Double-stranded DNA-nucleic acid dye complex-sensitized biocatalytic photosynthesis:Base pairing-switched regeneration of nicotinamide cofactor

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Journal of Energy Chemistry 2024年第7期94卷 618-624页

作者： zhen Dong Yanying Wang Dan Li Peng wu College of Chemistry Sichuan UniversityChengdu 610064SichuanChina Analytical&Testing Centre State Key Laboratory of Hydraulics and Mountain River EngineeringSichuan UniversityChengdu 610064SichuanChina School of Chemistry and Chemical Engineering Henan Normal UniversityXinxiang 453007HenanChina

Photo-biocatalysis,the combination of photosensitization and biocatalysis,is an emerging solution for sunlight-based renewable energy.It is thus important to develop light antennas with both good light har-vesting and efficient electron transfer.Herein,the intriguing electrical conductivity of dsDNA and its host effect(for nucleic acid dyes to harvest light)were explored simultaneously to develop a dsDNA-based light antenna for photo-biocatalysis.With SYBR Green I(SG)as the example of the nucleic acid dye,the proposed SG-dsDNA system was found to be capable for visible-light-driven reduced nicotinamide adenine dinucleotide(NADH)regeneration,and the turnover frequency of which(1.35 min^(-1))exceeded most of the existing photocatalytic systems.Since SG can only be hosted by dsDNA,meanwhile dsDNA can be formed through hybridization between single strand DNA and its complementary strand,the pro-posed system adds an extra control of the photocatalytic activity(DNA base pairing-based switch).When integrating the SG-dsDNA system with NADH-dependent horse liver alcohol dehydrogenase(HLADH),successful synthesis of 2-phenylpropanol(a crucial intermediates of profens manufacturing)was achieved.

关键词： Photosynthesis dsDNA SYBRGreenI TICT 2-phenylpropanol

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Integration of urchin-like MnCo_(2)O_(4)@C core–shell nanowire arrays within porous copper current collector for superior performance Li-ion battery anodes

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Rare Metals 2024年第2期43卷 599-611页

作者： Xue-ru Zhang Qiong wu Yong Zhang Xi-Fei Li Ting Xie Yu-Cheng wu Instrumental Analysis Center Hefei University of TechnologyHefei230009China Key Laboratory of Advanced Functional Materials and Devices of Anhui Province Hefei230009China School of Materials Science and Engineering Hefei University of TechnologyHefei230009China Institute of Advanced Electrochemical Energy Xi’an University of TechnologyXi’an710048China

Spinel MnCo_(2)O_(4) is a promising energy storage candidate as anode materials in lithium-ion batteries owing to synergistic effects of two intrinsic solid-state redox couples.However,low conductivity,poor rate capacity and rapid capacity fading have seriously impaired its practical applications.To overcome the inferiorities,urchin-like MnCo_(2)O_(4)@C core–shell nanowire arrays have been fabricated directly within a porous copper current collector via a facile hydrothermal method followed by a chemical vapor deposition carbonization process.In a typical nanowire,the core is composed of interconnected MnCo_(2)O_(4)nanoparticles and the shell shows as a thin amorphous carbon layer.The integrated MnCo_(2)O_(4)@C/Cu structure could act as working anodes without using additives or polymer binders.While MnCo_(2)O_(4)@C/Cu possesses slightly longer Li-ion insertion/desertion pathway than that of MnCo_(2)O_(4)/Cu,the carbon shell could effectively prevent the pulverization of MnCo_(2)O_(4) and lower down charge transfer resistance and actively participate in Li-ion cycles.The rearrangement of carbon atoms during lithiation/delithiation cycling could inhibit the formation of passive solid electrolyte interphase films.As a result,the MnCo_(2)O_(4)@C/Cu electrode presents superior rate capacity(600 mAh·g^(−1) at 1 A·g^(−1)) and better stability(797 mAh·g^(−1) after 200 cycles at 100 mA·g^(−1)).The excellent reversible Li ion storage capacity,cycling stability and rate capacity endow MnCo_(2)O_(4)@C/Cu great potential as stable and high output integrated anode materials in Li-ion batteries.

关键词： MnCo_(2)O_(4)@C Urchin-like nanowire arrays Core-shell Integrated anode materials Lithium-ion batteries

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