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Aurora B promotes the CENP-T–CENP-W interaction to guide accurate chromosome segregation in mitosis

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Journal of Molecular Cell Biology 2024年第2期16卷 11-23页

作者： Wei Liu zhen dou Chunyue Wang Gangyin Zhao Fengge Wu Chunli Wang Felix Aikhionbare Mingliang Ye Divine Mensah Sedzro zhenye Yang Chuanhai Fu Zhikai Wang Xinjiao Gao Xuebiao Yao Xiaoyu Song Xing Liu MOE Key Laboratory for Cellular Dynamics Center for Advanced Interdisciplinary Science and Biomedicine of IHMUniversity of Science and Technology of China School of Life SciencesHefei 230027China Keck Center for Cellular Dynamics and Organoids Plasticity AtlantaGA 30310USA Anhui Key Laboratory for Cellular Dynamics and Chemical Biology Hefei National Research Center for Interdisciplinary Sciences at the MicroscaleHefei 230026China National Chromatographic Research and Analysis Center Chinese Academy of SciencesDalian 116023China

Accurate chromosome segregation in mitosis depends on kinetochores that connect centromeric chromatin to spindle *** are captured by individual microtubules via a kinetochore constitutive centromere-associated network(CCAN)during chromosome *** contains 16 subunits,including CENP-W and ***,the molecular recognition and mitotic regulation of the CCAN assembly remain ***,we revealed that CENP-W binds to the histone fold domain and an uncharacterized N-terminal region of *** B phosphorylates CENP-W at threonine 60,which enhances the interaction between CENP-W and CENP-T to ensure robust metaphase chromosome alignment and accurate chromosome segregation in *** findings delineate a conserved signaling cascade that integrates protein phosphorylation with CCAN integrity for the maintenance of genomic stability.

关键词： mitosis kinetochore Aurora B CENP-T CENP-W phosphorylation

饮食模式改善多囊卵巢综合征患者生殖内分泌紊乱的研究进展

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中华生殖与避孕杂志 2024年第7期44卷 750-754页

作者：张允芝窦真王宝娟夏天天津中医药大学第一附属医院生殖医学科、国家中医针灸临床医学研究中心天津300193

饮食干预作为一种简便、易于实施的非药物治疗方法被国际指南推荐为多囊卵巢综合征(polycystic ovary syndrome,PCOS)患者的一线治疗方案。科学合理的饮食干预已经越来越受到人们的重视,一系列饮食模式,包括低升糖指数饮食、地中海饮食... 详细信息

饮食干预作为一种简便、易于实施的非药物治疗方法被国际指南推荐为多囊卵巢综合征(polycystic ovary syndrome,PCOS)患者的一线治疗方案。科学合理的饮食干预已经越来越受到人们的重视,一系列饮食模式,包括低升糖指数饮食、地中海饮食、生酮饮食、停止高血压饮食、限时饮食等已被证明可以降低PCOS女性体质量,改善生殖内分泌紊乱。本文从临床和基础实验研究等方面阐述了现有饮食模式改善PCOS女性生殖内分泌紊乱的临床症状及其作用机制,以完善饮食模式在PCOS治疗中的应用证据,为临床管理中提供最佳饮食建议。

关键词：多囊卵巢综合征生殖内分泌饮食模式饮食干预

葡萄糖转运蛋白对子宫内膜蜕膜化影响的研究进展

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中华生殖与避孕杂志 2024年第5期44卷 537-540页

作者：高娜窦真赵晓丽王宝娟夏天天津中医药大学第一附属医院生殖医学科、国家中医针灸临床医学研究中心天津300381

葡萄糖转运蛋白(glucose transporters,GLUTs)在子宫内膜中广泛表达,介导葡萄糖摄取,调控子宫内膜基质细胞的能量代谢及蜕膜免疫代谢等过程,并与蜕膜化过程中重要的信号通路相互作用,影响胚胎植入。本文将对蜕膜化过程中GLUTs的表达及... 详细信息

葡萄糖转运蛋白(glucose transporters,GLUTs)在子宫内膜中广泛表达,介导葡萄糖摄取,调控子宫内膜基质细胞的能量代谢及蜕膜免疫代谢等过程,并与蜕膜化过程中重要的信号通路相互作用,影响胚胎植入。本文将对蜕膜化过程中GLUTs的表达及其作用机制作一综述,以期为不孕症及妊娠相关疾病的防治提供理论依据。

关键词：葡萄糖转运蛋白子宫内膜基质细胞蜕膜化胚胎植入

不同热处理工艺对选区激光熔化TC4动载性能及各向异性的影响

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航空制造技术 2024年第6期67卷 100-108页

作者：窦振张安峰王豫跃黄胜吴梦杰王普强西安交通大学金属材料强度国家重点实验室西安710049 西安交通大学机械制造系统工程国家重点实验室西安710054

选区激光熔化成形的TC4合金断裂韧性较差,低周疲劳性能较低,各向异性明显。采用循环退火(700~950℃)和固溶时效相结合的方法对TC4成形件进行热处理,利用光学显微镜、扫描电子显微镜、低周疲劳试验机等手段研究热处理对SLM TC4成形件显... 详细信息

选区激光熔化成形的TC4合金断裂韧性较差,低周疲劳性能较低,各向异性明显。采用循环退火(700~950℃)和固溶时效相结合的方法对TC4成形件进行热处理,利用光学显微镜、扫描电子显微镜、低周疲劳试验机等手段研究热处理对SLM TC4成形件显微组织和力学性能的影响。研究表明,SLM TC4微观组织由马氏体α′和马氏体α″组成,断裂韧性值为36.4 MPa·m0.5,断裂韧性各向异性达25.7%;热处理后部分板条α相分解,产生等轴α相和二次α相;经过700~950℃循环5次后固溶+550℃时效后SLM TC4的断裂韧性值为96.0 MPa·m0.5,断裂韧性的各向异性为1.4%。通过比较热处理样件和锻件低周疲劳性能得出结论,当应变幅≥0.9%时,热处理件低周疲劳性能高于锻件;当应变幅≤1%时,920℃循环退火+550℃固溶时效的低周疲劳性能高于920℃循环退火。

关键词：选区激光熔化(SLM) TC4合金热处理断裂韧性低周疲劳性能各向异性

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模态分解的电能质量检测探究实验教学实践

计算机仿真 2024年第1期41卷 293-300页

作者：窦震海王雅静梅宇张祥珂山东理工大学电气与电子工程学院山东淄博255091

针对“现代信号处理”课程的教学难点,以模态分解法(EMD)的电能质量扰动信号检测为例,进行了探究性实验教学仿真与实践。在教学过程中,引导学生对传统EMD电能质量检测法精度偏低的问题进行探究。然后根据探究结果,融合SVD滤波、ICEEMDA... 详细信息

针对“现代信号处理”课程的教学难点,以模态分解法(EMD)的电能质量扰动信号检测为例,进行了探究性实验教学仿真与实践。在教学过程中,引导学生对传统EMD电能质量检测法精度偏低的问题进行探究。然后根据探究结果,融合SVD滤波、ICEEMDAN及排列熵(PE)等多种现代信号处理技术,提出了SVD-ICEEMDAN-PE的电能质量扰动检测方法,能够提高精测精度。在此基础上,设计信号降噪、算法性能分析、扰动信号检测、实验信号检测等多个仿真对方法的有效性进行了验证。探究性实验教学是理论教学的延伸和拓展,教学过程注重以学生为中心,引导学生体会思考-探索-解决问题的科研方法,从而激发学生求学—求知—钻研的科研精神。

关键词：现代信号处理探究性实验电能质量经验模态分解

Protein kinase TTK interacts and co-localizes with CENP-E to the kinetochore of human cells

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Chinese Science Bulletin 2002年第23期47卷 2005-2009页

作者： Jie Zhang Chuanhai Fu Yong Miao zhen dou Xuebiao Yao Univ Sci & Technol China Lab Cell Dynam Hefei 230027 Peoples R China

Spindle checkpoint is an important biochemical signaling cascade during mitosis which monitors the fidelity of chromosome segregation, and is mediated by protein kinases Mpsl and Bubl/BubRl. Our recent studies show that kinesin-related motor protein CENP-E interacts with BubRl and participates in spindle checkpoint signaling. To elucidate the molecular mechanisms underlying spindle checkpoint signaling, we carried out proteomic dissection of human cell kinetochore and revealed protein kinase TTK, human homologue of yeast Mpsl. Our studies show that TTK is localized to the kinetochore of human cells, and interacts with CENP-E, suggesting that TTK may play an important role in chromosome segregation during mitosis.

关键词： spindle checkpoint TTK protein kinase CENP-E kinetochore.

A novel genome-wide full-length kinesin prediction analysis reveals additional mammalian kinesins

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Chinese Science Bulletin 2006年第15期51卷 1836-1847页

作者： XUE Yu LIU Dan FU Chuanhai dou zhen ZHOU Qing YAO Xuebiao Laboratory of Cellular Dynamics University of Science & Technology of China/Hefei National Laboratory Hefei 230027 China College of Agriculture Zhejiang University Hangzhou 310003 China

Kinesin superfamily of microtubule- based motor orchestrates a variety of cellular proc- esses. Recent availability of mammalian genomes has enabled analyses of kinesins on the whole ge- nome. Here we present a novel full-length kinesin prediction program (FKPP) for mammalian kinesin gene discovery based on a comparative genomics approach. Contrary to previous predictions of 94 kinesins, we identify a total of 134 potentially kinesin genes from mammalian genomes, including 45 from mouse, 45 from rat and 44 from human. In addition, FKPP synthesizes 25 potentially full-length mam- malian kinesins based on the partial sequences in the database. Surprisingly, FKPP reveals that full-length human CENP-E contains 2701 aa rather than 2663 aa in the database. Experimentation using sequence specific antibody and cDNA sequencing of human CENP-E validates the accuracy of FKPP. Given the remarkable computing efficiency and accuracy of FKPP, we reclassify the mammalian kinesin super- family. Since current databases contain many in- complete sequences, FKPP may provide a novel approach for molecular delineation of kinesins and other protein families.

关键词：驱动蛋白基因组 CENP-E FKPP

Mechanisms and regulation underlying membraneless organelle plasticity control

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Journal of Molecular Cell Biology 2021年第4期13卷 239-258页

作者： Hazrat Ismail Xu Liu Fengrui Yang Junying Li Ayesha Zahid zhen dou Xing Liu Xuebiao Yao MOE Key Laboratory for Membraneless Organelles&Cellular Dynamics and CAS Center for Excellence in Molecular Cell Science Hefei National Laboratory for Physical Sciences at the MicroscaleUniversity of Science and Technology of ChinaHefei 230027China Keck Center for Organoids Plasticity Control AtlantaGA 30310USA Anhui Key Laboratory for Cellular Dynamics&Chemical Biology Hefei 230027China

Evolution has enabled living cells to adopt their structural and functional complexity by organizing intricate cellular compartments,such as membrane-bound and membraneless organelles(MLOs),for spatiotemporal catalysis of physiochemical reactions essential for cell plasticity *** evidence and view support the notion that MLOs are built by multivalent interactions of biomolecules via phase separation and transition *** healthy cells,dynamic chemical modifications regulate MLO plasticity,and reversible phase separation is essential for cell *** evidence revealed that aberrant phase separation results in numerous neurodegenerative disorders,cancer,and other *** this review,we provide molecular underpinnings on(i)mechanistic understanding of phase separation,(ii)unifying structural and mechanistic principles that underlie this phenomenon,(iii)various mechanisms that are used by cells for the regulation of phase separation,and(iv)emerging therapeutic and other applications.

关键词： liquid-liquid phase separation membraneless organelles biomolecular condensates intrinsically disordered proteins post-translational modifications

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侵袭性炎性纤维性息肉1例并文献报道

诊断病理学杂志 2024年第8期31卷 780-783页

作者：窦振华叶承祖杨冰洪杨文圣中国人民解放军陆军第七十三集团军医院病理科福建厦门361003

目的探讨侵袭性炎性纤维性息肉(IFP)的临床病理特征、诊断、鉴别诊断、治疗及预后。方法回顾性分析1例侵袭性炎性纤维性息肉的临床病理特征,并结合相关文献进行讨论。结果患者男性,62岁,因小肠梗阻行部分小肠切除术,术后巨检于肠壁见一... 详细信息

目的探讨侵袭性炎性纤维性息肉(IFP)的临床病理特征、诊断、鉴别诊断、治疗及预后。方法回顾性分析1例侵袭性炎性纤维性息肉的临床病理特征,并结合相关文献进行讨论。结果患者男性,62岁,因小肠梗阻行部分小肠切除术,术后巨检于肠壁见一隆起型肿物,大小4.5 cm×3 cm×3 cm。镜下见肿瘤细胞呈胖梭形、星芒状,排列杂乱,疏密不均,间质显示突出的血管及嗜酸性粒细胞浸润,肿瘤主体位于黏膜下层,并浸润破坏肠壁全层。肿瘤细胞弥漫表达vimentin,局部表达CD34、Actin及SMA。KIT基因和PDGFRA基因均无突变。结论侵犯肠壁全层的炎性纤维性息肉是一种罕见的肿瘤。诊断时需结合组织学及免疫组化,必要时可辅助基因检测。其治疗和预后情况仍需大样本研究和长期随访。

关键词：炎性纤维性息肉临床病理特征诊断鉴别诊断