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Development of aggregated state chemistry accelerated by aggregation-induced emission

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National Science Review 2021年第6期8卷 20-22页

作者： Yujun xie zhen Li Institute of Molecular Aggregation Science Tianjin University Joint School of National University of Singapore and Tianjin University International Campus of Tianjin University Department of Chemistry Wuhan University

Light is an essential ingredient of human civilization, and has been tightly associated with society and everyone’s daily life. Light can be emitted from various kinds of luminophores, and the concept of aggregation-induced emission(AIE)was a breakthrough in the field of organic light-emitting materials, which experienced stages of pursuing truth, discarding prejudices and creating new knowledge.

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Explore the dominant factor in prime editing via a view of DNA processing

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Synthetic and Systems Biotechnology 2023年第3期8卷 371-377页

作者： Zhimeng Xu Dacheng Ma Houzhen Su Xiaodong Jia Yinqing Li Yinying Lu Zhen Xie MOE Key Laboratory of Bioinformatics and Bioinformatics Division Center for Synthetic and System BiologyDepartment of AutomationBeijing National Research Center for Information Science and TechnologyTsinghua UniversityBeijing100084China Research Center for Biological Computation Zhejiang LaboratoryHangzhou311100China Comprehensive Liver Cancer Centre The Fifth Medical Center of PLA General HospitalBeijingChina School of Pharmaceutical Sciences IDG/McGovern Institute for Brain ResearchTsinghua UniversityBeijingChina

Prime editing is a revolutionary gene-editing method that is capable of introducing insertions,deletions and base substitutions into the genome.However,the editing efficiency of Prime Editor(PE)is limited by the DNA repair process.Here,we show that overexpression of the flap structure-specific endonuclease 1(FEN1)and the DNA ligase 1(LIG1)increases the efficiency of prime editing,which is similar to the dominant negative mutL homolog 1(MLH1dn).In addition,MLH1 is still the dominant factor over FEN1 and LIG1 in prime editing.Our results help to further understand the relationship of proteins involved in prime editing and envisage future directions for the development of PE.

关键词： process. prime directions

Defective/Doped Graphene-Based Materials as Cathodes for Metal-Air Batteries

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Energy & Environmental Materials 2022年第4期5卷 1103-1116页

作者： Qinming Zhang Chengyi Wang Zhaojun xie zhen Zhou School of Materials Science and Engineering Institute of New Energy Material ChemistryNankai UniversityTianjin 300350China Engineering Research Center of Advanced Functional Material Manufacturing of Ministry of Education School of Chemical EngineeringZhengzhou UniversityZhengzhou 450001China

Graphene,as a proof-of-concept two-dimensional material,has proven to have excellent physical and chemical properties.Its derivatives,such as defective or doped graphene,are also applied as catalytic materials for metal-air batteries(MABs).MABs have been recognized as possible candidates for new-generation energy storage systems due to their ultra-high theoretical energy density.So far,graphene and its derivatives with optimized structures have been widely explored to improve the electrochemical performance in MABs.Generally speaking,perfect graphene crystalline is inert for many catalytic processes,while defects and heteroatoms can endow graphene with high activity for many electrocatalytic reactions.Under this circumstance,recent progress is summarized for defective/doped graphene as air cathodes in aqueous or organic MABs,which are actually different electrochemical systems with distinct requirements for air cathodes.Also,the relationship is clarified between graphene defects/doping and electrocatalytic mechanisms that can be the guidance for catalyst design.Future directions are also prospected for the development of graphene-based MAB cathodes.

关键词： defective/doped graphene electrocatalysts metal-air batteries OER ORR

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Protein post-translational modification by lysine succinylation:Biochemistry,biological implications,and therapeutic opportunities

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Genes & Diseases 2023年第4期10卷 1242-1262页

作者： Guo Zhao Junfeng zhen Xinyuan Liu Jinshuai Guo Duanduan Li Jianping xie Longxiang xie Institute of Biomedical Informatics Bioinformatics CenterHenan Provincial Engineering Center for Tumor Molecular MedicineSchool of Basic Medical SciencesHenan UniversityKaifengHenan 475004China State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area Key Laboratory of Eco-Environments in Three Gorges Reservoir RegionInstitute of Modern BiopharmaceuticalsMinistry of EducationSchool of Life SciencesSouthwest UniversityChongqing 400715China

Lysine succinylation(Ksuc)is a novel protein post-translational modification(PTM)wherein a succinyl group modifies a lysine residue.Ksuc leads to significant chemical and struc-tural changes to the modified protein.Recent studies have shown that Ksuc might play an important role in organism physiology and some pathophysiological processes,such as tumor-igenesis and metabolic diseases.To provide an understanding of the molecular mechanism and functions of Ksuc in different organisms,we reviewed the current literature about Ksuc,mainly summarizing the research advances in eukaryotes and prokaryotes based on both traditional study methods and site prediction tools.We also discussed inhibitors or activators associated with Ksuc that may contribute to proteomic studies and could be useful in future clinical prac-tice.A deeper understanding of Ksuc may shed new light on life science at the protein level and could lead to novel therapeutic strategies for various diseases.

关键词： Disease Eukaryotes Lysine succinylation Physiology Prokaryotes Protein post-translational modification

Construction of a CRISPR-based paired-sgRNA library for chromosomal deletion of long non-coding RNAs

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Quantitative Biology 2020年第1期8卷 31-42页

作者： Minzhen Tao Qiaochu Mu Yurui Zhang Zhen Xie MOE Key Laboratory of Bioinformatics and Bioinformatics Division Center for Synthetic and System BiologyDepartment of AutomationBeijing National Research Center for Information Science and TechnologyTsinghua UniversityBeijing 100084China Zhejiang Tmall Techonogy Co. LtdYuhang DistrictHangzhou 311121China Beiing Syngentech Co..Ltd Zhongguancun Life Science ParkChangping DistrictBeijing 102206China

Background:Derived from an adaptive bacterial immune system,the clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated 9(Cas9)system has shown great potential in high-throughput functional genomic screening,especially for protein-coding genes.However,it is still challenging to apply the similar strategy to study non-coding genomic elements such as long non-coding RNAs(lncRNAs)or clusters of microRNAs,because short insertions or deletions may not be sufficient to generate loss-of-function phenotypes.Methods:Here,we presented a systematic strategy for designing a CRISPR-based paired-sgRNA library for highthroughput screening in non-coding regions.Due to the abundance of lncRNAs and their diverse regulatory roles in vivo,we repurposed microarray datasets to select 600 highly expressed lncRNAs in non-small-cell lung cancer and designed two schemes for lncRNA deletion with^20 paired-sgRNAs for each lncRNA.Through Golden-Gate assembly,we generated a pooled CRISPR-based library with a total of 12,878 sgRNA pairs.Results:Over 80%of paired-sgRNAs were recovered from final pooled library with a relarively even distribution.Cleavage efficiency of sgRNA pairs was validated through experiments of transient transfection and viral infection.Moreover,randomly selected paired-sgRNAs showed that efficient deletion of genomic DNA could be achieved with a deletion size within the range of 500 to 3000 bp.Conclusions:In summary,we have demonstrated a strategy to design and construct a pooled paired-sgRNA library to generate genomic deletion in the lncRNA regions,validated their deletion efficiency and explored the relationship of deletion efficiency with respect to deletion size.This method would be also suitable for investigation of other uncharacterized non-coding genomic regions in mammalian cells in an efficient and cost-efTective manner.

关键词： CRISPR/Cas9 paired-sgRNA chromosomal deletion non-coding genomic elements lncRNA

Specialized Microglia Resolve Neuropathic Pain in the Spinal Cord

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Neuroscience Bulletin 2023年第1期39卷 173-175页

作者： Jing Yang Shulan xie Shengmei Zhu zhen-Zhong Xu Department of Anesthesiology and Department of Neurobiology of the First Affiliated Hospital Zhejiang University School of MedicineHangzhou310058China NHC and CAMS Key Laboratory of Medical Neurobiology MOE Frontier Science Center for Brain Research and Brain–Machine IntegrationSchool of Brain Science and Brain MedicineZhejiang UniversityHangzhou310058China

Neuropathic pain is a major health problem affecting up to 10%of the general population,resulting from a lesion or dysfunction of the somatosensory nervous system,including peripheral nerve injury(PNI)^([1]).However,neuropathic pain is difficult to treat because of its obscure mechanisms.Diverse types of neuropathic pain have been modelled in rodents to study its underlying mechanisms and identify novel therapeutic targets.

关键词： pain mechanisms Spinal

Sequence Impedance Model and Resonance Damping of Voltage Source based DFIG

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Sequence Impedance Model and Resonance Damping of Voltage So...

Novel Bilayer-Shelled N,O-Doped Hollow Porous Carbon Microspheres as High Performance Anode for Potassium-Ion Hybrid Capacitors

第32届中国控制与决策会议

作者： CHEN Junhe xie zhen National and Local Joint Engineering Laboratory for Renewable Energy Access to Grid Technology Hefei University of technology

ISBN: (纸本)9781728158549

When a voltage-source based doubly fed induction generator（DFIG） wind power system is connected to a parallel-compensated weak grid, due to the impedance interaction between the two systems, the problem of medium-high frequency resonance is prone to occur. In order to solve the problem of medium and high frequency resonance and improve the stability of the operation of the voltage-source based DFIG wind power system when connected to the parallel compensation weak grid, this paper proposes an active damping resonance suppression control strategy. First, the harmonic impedance linearization method was used to establish the sequence impedance model of the voltage-source based DFIG. Then, based on the impedance model, the stator current is fed forward into the current inner loop of the generator-side converter in real time, and the combined damping of virtual positive resistance and positive capacitance is used to reshape the DFIG wind power system impedance to achieve medium-high frequencies. Purpose of resonance suppression. Experimental results verify the effectiveness of the proposed DFIG system damping control strategy.

关键词： Voltage-source based DFIG Active damping resonance Medium-high frequency resonance

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Nano-Micro Letters 2023年第9期15卷 423-435页

作者： zhen Pan Yong Qian Yang Li Xiaoning xie Ning Lin Yitai Qian Department of Applied Chemistry University of Science and Technology of ChinaHefei 230026People’s Republic of China Yongjiang Laboratory Ningbo 315202People’s Republic of China China National Building Material Design&Research Institute Co. Ltd.No.208Huazhong RoadGongshu DistrictHangzhou 310022People’s Republic of China

With the advantages of high energy/power density,long cycling life and low cost,dual-carbon potassium ion hybrid capacitors(PIHCs)have great potential in the field of energy storage.Here,a novel bilayer-shelled N,O-doped hollow porous carbon microspheres(NOHPC)anode has been prepared by a self-template method,which is consisted of a dense thin shell and a hollow porous spherical core.Excitingly,the NOHPC anode possesses a high K-storage capacity of 325.9 mA h g^(−1)at 0.1 A g^(−1)and a capacity of 201.1 mAh g^(−1)at 5 A g^(−1)after 6000 cycles.In combination with ex situ characterizations and density functional theory calculations,the high reversible capacity has been demonstrated to be attributed to the co-doping of N/O heteroatoms and porous structure improved K+adsorption and intercalation capabilities,and the stable long-cycling performance originating from the bilayer-shelled hollow porous carbon sphere structure.Meanwhile,the hollow porous activated carbon microspheres(HPAC)cathode with a high specific surface area(1472.65 m^(2)g^(−1))deriving from etching NOHPC with KOH,contributing to a high electrochemical adsorption capacity of 71.2 mAh g^(−1)at 1 A g^(−1).Notably,the NOHPC//HPAC PIHC delivers a high energy density of 90.1 Wh kg^(−1)at a power density of 939.6 W kg^(−1)after 6000 consecutive charge-discharge cycles.

关键词： Self-template method Bilayer-shelled hollow porous structure N,O-doped carbon microspheres Dual-carbon potassium‐ion hybrid capacitor

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Rational design of a compact CRISPR/Cas system for gene editing and transcriptional regulation

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Rational design of a compact CRISPR/Cas system for gene edit...