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Cell Research 2001年第1期11卷 61-67页

作者： MA YINGHUA, JIA HUA HU, WEN JUAN ZHAO, JIAN FEI, YUN YU, XIAO GANG ZHOU, zhen tong MEI, LI HE GUO (Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China) (Shanghai Inst Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Shanghai Institute of Physiology Chinese Academy of Sciences Shanghai China

γ-aminobutyric acid (GABA) is the principal inhibitory neurotransmitter, and the GABAergic synaptic transmission is normally terminated by the rapid uptake through GABA transporters. With transgenic mice ubiquitously overexpressing GABA transporter subtype I (GAT1), the present study explored the pathophysiological role of GAT1 in epileptogenesis. Though displaying no spontaneous seizure activity, these mice exhibit altered electroencephalographic patterns and increased susceptibility to seizure induced by kainic acid. In addition, the GABAA receptor and glutamate transporters are up-regulated in transgenic mice, which perhaps reflects a compensatory or corrective change to the elevated level of GAT1. These preliminary findings support the hypothesis that excitatory and inhibitory neurotransmission, and seizure susceptibility can be altered by neurotransmitter transporters.

关键词： γ-aminobutyric acid transporter seizure susceptibility kainic acid electroencephalography transgenic mice

γ-Aminobutyric acid transporter (GAT1) overexpression in mouse affects the testicular morphology

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Cell Research 2000年第1期10卷 59-69页

作者： MA YING HUA JIA HUA HU XIAO GANG ZHOU zhen tong MEI JIAN FEI LI HE GUO(Shanghai Institute of Cell Biology, Chinese Academy of Science, Shanghai 200031, China Shanghai Institute of Physiology, Chinese Academy of Science, Shanghai 200031, China) Shanghai Institute of Cell Biology Chinese Academy of Science Shanghai China Shanghai Institute of Physiology Chinese Academy of Science Shanghai China

γ-Aminobutyric acid and GABAergic receptors were previously reported to be distributed in reproductive systems besides CNS and predicted to participate in the modulation of testicular function. γ-Aminobutyric acid transporter was implicated to be involved in this process. However, the potential role of γ-aminobutyric transporter in testis has not been explored. In this study, we investigated the existence of mouse γ-aminobutyric acid transporter subtype I (mGAT1) in testis. Wild-type and transgenic mice, which overexpressing mGAT1 in a variety of tissues, especially in testis, were primarily studied to approach the profile of mGAT1 in testis. Mice with overexpressed mGAT1 develop normally but with reduced mass and size of testis as compared with wild-type. Testicular morphology of transgenic mice exhibited overt abnormalities including focal damage of the spermatogenic epithelium accompanied by capillaries proliferation and increased diameter of seminiferous tubules lumen. Reduced number of spermatids was also found in some seminiferous tubules. Our results clearly demonstrate the presence of GAT1 in mouse testis and imply that GAT1 is possibly involved in testicular function.

关键词： γ-aminobutyric acid transporter testicular morphology mouse.

Robust free-space optical frequency transfer in time-varying link distances conditions

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Chinese Physics B 2024年第2期33卷 271-276页

作者：童周刘雷王家亮操前金志成应康韩申生韩正甫桂有珍 School of Physical Sciences University of Science and Technology of ChinaHefei 230000China Key Laboratory for Quantum Optics Shanghai Institute of Optics and Fine MechanicsChinese Academy of SciencesShanghai 201800China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China Key Laboratory of Space Laser Communication and Detection Technology Shanghai Institute of Optics and Fine MechanicsChinese Academy of SciencesShanghai 201800China Hangzhou Institute for Advanced Study University of Chinese Academy of SciencesHangzhou 310024China

Future inter-satellite clock comparison on high orbit will require optical time and frequency transmission technology between moving ***,we demonstrate robust optical frequency transmission under the condition of variable link *** variable link is accomplished by the relative motion of a single telescope fixed on the experimental platform to a corner-cube reflector(CCR)installed on a sliding *** acousto–optic modulators with different frequencies are used to separate forward signal from backward *** active phase noise suppression,when the CCR moves back and forth at a constant velocity of 20 cm/s and an acceleration of 20 cm/s^(2),we achieve the best frequency stability of 1.9×10^(-16) at 1 s and 7.9×10^(-19) at 1000 s *** work paves the way for future studying optical frequency transfer between ultra-high-orbit satellites.

关键词： free-space optical frequency transfer variable link distance

基于改进Faster R-CNN的接触网管帽目标定位算法

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激光与光电子学进展 2022年第4期59卷 132-142页

作者：顾桂梅陈充余晓宁张存俊仝甄梅小芸兰州交通大学自动化与电气工程学院甘肃兰州730070 中国铁路兰州局集团有限公司甘肃兰州730030 国网甘肃省电力公司庆阳供电公司甘肃庆阳745000

为了改善接触网管帽这类小尺度部件在故障检测过程中定位困难的情况,提出一种基于改进Faster R-CNN的接触网管帽目标定位算法。通过K均值聚类算法(K-means)对region proposal network(RPN)层中生成anchor boxes的比例及面积进行改进,所... 详细信息

为了改善接触网管帽这类小尺度部件在故障检测过程中定位困难的情况,提出一种基于改进Faster R-CNN的接触网管帽目标定位算法。通过K均值聚类算法(K-means)对region proposal network(RPN)层中生成anchor boxes的比例及面积进行改进,所提算法在定位接触网管帽这类小部件上具有较好的表现。并通过比较VGG16、resnet50、resnet101、resnet152等4种特征提取网络在原始及改进的Faster R-CNN上定位管帽的准确率、召回率、准确率和召回率的调和平均F_(1)、单张检测时间等指标来选择最优特征提取网络。实验结果表明,基于resnet50的改进Faster R-CNN深度网络模型在接触网管帽定位中具有明显的优势,召回率为89.78%,定位准确率可以达到83.16%,F_(1)值为86.34%,单张检测时间为0.283 s。

关键词：图像处理接触网管帽定位 Faster R-CNN K均值聚类算法深度学习

Heme oxygenase 1 linked to inactivation of subchondral osteoclasts in osteoarthritis

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第6期25卷 513-528页

作者： Miao CHU Guangdong CHEN Kai CHEN Pengfei ZHU zhen WANG Zhonglai QIAN Huaqiang TAO Yaozeng XU Dechun GENG Department of Orthopedics the First Affiliated Hospital of Soochow UniversitySuzhou 215006China Department of Orthopedics Yixing People’s HospitalYixing 214200China Department of Orthopedics Hai’an People’s HospitalHai’an 226600China Department of Orthopedics Suzhou Kowloon HospitalShanghai Jiao Tong University School of MedicineSuzhou 215028China

Osteoarthritis(OA)is a chronic progressive osteoarthropathy in the *** activation plays a crucial role in the occurrence of subchondral bone loss in early ***,the specific mechanism of osteoclast differentiation in OA remains *** our study,gene expression profiles related to OA disease progression and osteoclast activation were screened from the Gene Expression Omnibus(GEO)***2R and Funrich analysis tools were employed to find differentially expressed genes(DEGs).Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses demonstrated that chemical carcinogenesis,reactive oxygen species(ROS),and response to oxidative stress were mainly involved in osteoclast differentiation in OA subchondral ***,fourteen DEGs that are associated with oxidative stress were *** first ranked differential gene,heme oxygenase 1(HMOX1),was selected for further *** results showed that osteoclast activation in the pathogenesis of OA subchondral bone is accompanied by the downregulation of *** was revealed to inhibit osteoclastogenesis by targeting HMOX1 and upregulating the expression of antioxidant protein in ***,carnosol was found to alleviate the severity of OA by inhibiting the activation of subchondral osteoclasts in *** research indicated that the activation of osteoclasts due to subchondral bone redox dysplasia may serve as a significant pathway for the advancement of *** HMOX1 in subchondral osteoclasts may offer novel insights for the treatment of early OA.

关键词： Osteoclast Oxidative stress Osteoarthritis(OA) Heme oxygenase 1(HMOX1) Carnosol

子宫颈癌HeLa细胞中Bcrp1~+细胞的生物学特性

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中华妇产科杂志 2010年第7期45卷 525-529页

作者：张松灵于晓伟魏振佟付艳吉林大学第一医院妇产科

目的探讨宫颈癌HeLa细胞中Bcrp1~+细胞的生物学特性。方法采用细胞免疫荧光标记及流式细胞仪分选HeLa细胞中Bcrp1~+及Bcrp1~-细胞,透射电镜观察两种表型细胞的形态,倒置显微镜观察两种表型细胞培养不同时间(24、48、72 h)后的生长情况;... 详细信息

目的探讨宫颈癌HeLa细胞中Bcrp1~+细胞的生物学特性。方法采用细胞免疫荧光标记及流式细胞仪分选HeLa细胞中Bcrp1~+及Bcrp1~-细胞,透射电镜观察两种表型细胞的形态,倒置显微镜观察两种表型细胞培养不同时间(24、48、72 h)后的生长情况;采用流式细胞仪检测两种表型细胞的细胞周期及细胞凋亡情况;采用蛋白印迹法检测两种表型细胞中增殖周期相关蛋白——增殖细胞核抗原(PCNA)及半胱氨酸天冬氨酸蛋白酶3(caspase-3)的表达情况(结果均以"表达丰度"表示)。结果 (1)分选结果显示,宫颈癌HeLa细胞中,Bcrp1~+细胞占7.1%,Bcrp1~-细胞占92.9%。透射电镜观察,与Bcrp1~-细胞相比较,Bcrp1~+细胞核大、核仁明显、胞质内线粒体丰富、粗面内质网增多。倒置显微镜观察,分选后的Bcrp1~+细胞迅速进入分裂状态,培养24 h即贴壁生长,而Bcrp1~-细胞培养48 h方有少量贴壁生长;培养24、48、72 h时,Bcrp1~+贴壁细胞分别占72.8%、81.1%、80.4%,Bcrp1~-贴壁细胞分别占3.3%、18.7%、12.6%,各个时间点两种表型细胞的贴壁细胞所占百分比分别比较,差异均有统计学意义(P<0.05)。(2)流式细胞仪检测显示,Bcrp1~+细胞中S期细胞比例(54.1%)和增殖指数(55.6%)均高于Bcrp1~-细胞中S期细胞比例(21.1%)和增殖指数(47.0%),差异均有统计学意义(P<0.05);而Bcrp1~-细胞中G/G及G/M期细胞比例(分别为53.0%、25.9%)均高于Bcrp1~+细胞(分别为44.4%、1.5%),差异均有统计学意义(P<0.05)。Bcrp1~+细胞的凋亡率为0.2%,Bcrp1~-细胞为5.3%,两者比较,差异有统计学意义(P<0.05)。(3)蛋白印迹法检测显示,在Bcrp1~+细胞中PCNA蛋白的表达水平(表达丰度为3140)高于Bcrp1~-细胞(表达丰度为2255),而caspase-3蛋白的表达水平(表达丰度为1970)低于Bcrp1~-细胞(表达丰度为3551),分别比较,差异均有统计学意义(P<0.05)。结论宫颈癌HeLa细胞中Bcrp1~+细胞具有较强的抗凋亡能力及旺盛的生命力,高表达PCNA蛋白、低表达caspase-3蛋白,是一群具有肿瘤干细胞特征的细胞。Bcrp1~+的HeLa细胞中可能含有宫颈癌干细胞。

关键词：宫颈肿瘤 HeLa细胞肿瘤蛋白质类 ATP结合匣式转运子肿瘤干细胞肿瘤标记,生物学

Fatigue Test of Domestic Manufactured Reactor Pressure Vessel Steel

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Fatigue Test of Domestic Manufactured Reactor Pressure Vesse...

作者： ZHONG Wei-hua tong zhen-feng NING Guang-sheng YU Bin-tao Department of Reactor Engineering Research and Design

The CAP 1400 will be built by our country,after the self-dependent innovation work on the imported technology of AP1000,which is a 3rd generation ***,the design of CAP 1400 key equipment is ongoing,and the fatigue design of the domestic manufactured key equipment,such as reactor pressure vessel(RPV),is found to be a main problem in the design work,as the fatigue data is *** the

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CO2分离式热管在数据中心的应用

制冷学报 2021年第1期42卷 67-73页

作者：佟振李晓瑞青岛理工大学环境与市政工程学院青岛266033

本文通过实验研究了应用于数据中心的CO2分离式热管系统,对比分析了CO2热管与R22热管的最大传热能力、总传热热阻和驱动温差,结果表明:在相同充液率下,CO2热管的最大传热能力明显大于R22热管,当上升管和下降管管径为9 mm时,CO2热管和R2... 详细信息

本文通过实验研究了应用于数据中心的CO2分离式热管系统,对比分析了CO2热管与R22热管的最大传热能力、总传热热阻和驱动温差,结果表明:在相同充液率下,CO2热管的最大传热能力明显大于R22热管,当上升管和下降管管径为9 mm时,CO2热管和R22热管的最大传热能力分别为3300 W和1500 W,当管径为12 mm时,CO2热管和R22热管的最大传热能力分别为5400 W和2200 W;CO2热管的正常负荷范围大于R22热管,但总传热热阻小于R22热管;不同传热量下,与R22热管相比,CO2热管所需的驱动温差平均低4℃,即相同条件下CO2热管系统所需的冷源温度可以提高4℃。以小型数据机房为例,结合上海气候条件计算得出,采用CO2热管系统的年耗电量比R22热管系统减少7.425×105 kW·h,比集中送风空调系统减少3.182×106 kW·h。

关键词：分离式热管 CO2 数据中心充液率

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一种面向储粮微环境的GPSO改进算法研究

中国粮油学报 2015年第12期30卷 102-105,113页

作者：吴建军甄彤祝玉华河南工业大学郑州450001

粮食作为一种特殊及复杂的生命体,储藏粮堆内部微环境的变化规律异常复杂,准确掌握粮堆内部温度场的变化是个难题。本研究通过对粒子群算法惯性权重和目标函数的修改,提出一种面向储粮微环境的GPSO改进算法,结果表明,改进算法在粒子分... 详细信息

粮食作为一种特殊及复杂的生命体,储藏粮堆内部微环境的变化规律异常复杂,准确掌握粮堆内部温度场的变化是个难题。本研究通过对粒子群算法惯性权重和目标函数的修改,提出一种面向储粮微环境的GPSO改进算法,结果表明,改进算法在粒子分布较为分散时,使得粒子群收敛速度较快,迭代曲线平稳,全局搜索能力强。在粒子分布较为密集时粒子的速度较为缓慢,其局部寻优能力强。该算法与其他算法对比分析,表明对储粮温度预测具有良好的结果,预测的拟合程度很高。

关键词：储粮微环境湿热传递预测模型 GPSO

PEG浓度对锂藻土分散液的再凝胶化及屈服行为的影响

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高等学校化学学报 2014年第10期35卷 2252-2257页

作者：疏瑞文孙尉翔刘新星童真华南理工大学材料科学研究所发光材料与器件国家重点实验室广州510640

通过离子电导率和pH考察了锂藻土自身释放的Na+浓度,说明未经透析或离子交换处理的cw=30mg/mL的锂藻土分散液静置老化后形成的软固体是反应置限团簇聚集(RLCA)的胶体凝胶.通过剪切流变方法研究了聚乙二醇(PEG)浓度cp对锂藻土/PEG分散液... 详细信息

通过离子电导率和pH考察了锂藻土自身释放的Na+浓度,说明未经透析或离子交换处理的cw=30mg/mL的锂藻土分散液静置老化后形成的软固体是反应置限团簇聚集(RLCA)的胶体凝胶.通过剪切流变方法研究了聚乙二醇(PEG)浓度cp对锂藻土/PEG分散液体系的线性和非线性黏弹性的影响.小幅振荡剪切(SAOS)频率扫描结果表明,随着cp增加,锂藻土发生'RLCA凝胶-黏弹性液体-排空(Depletion)凝胶'转变,可观察到该体系的再凝胶化行为.稳态剪切和大振幅剪切实验结果表明,锂藻土/PEG分散体系的再凝胶化的非线性流变学行为也有体现.

关键词：锂藻土聚乙二醇再凝胶化屈服行为小幅振荡剪切反应置限团簇聚集