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Agricultural Sciences in China 2010年第1期9卷 64-70页

作者： JIANG Xuan-li kang zhen-sheng Department of Plant Protection Agricultural College Guizhou University Guiyang 550025 P.R.China Plant Protection College Northwest A&F University Yangling 712100 P.R.China

Ultrastructural changes in both pathogen and host cells in the interaction between Puccinia striiformis and wheat cultivar （Libellula） with slow-rusting resistance were observed by transmission electron microscopy. Observations revealed marked changes in ultrastructure of both pathogen and host cells. In the pathogen respect, there were many vesicles appeared in the intercellular hyphae and gradually fused into bigger vacuoles, a number of fat drops and electron-dense granules accumulated, mitochondria became swollen and some of them degraded into vesicles, and the plasmalemma of intercellular hyphae became dark. In the haustoria, the cytoplasm degraded gradually and developed a vacuole in the center, fat drops increased, the extrahaustorial matrix widened with a great amount of electron-dense fibrillar and granular materials, and most of the haustoria died with in conjunction with the disappearance of fat drops and other organelles. Structural defense of the host, including formation of cell wall apposition, collar and papilla, occurred in the host respect. Host resistance expression and cytological features occurring in the slow-rusting resistance were discussed.

关键词： wheat Puccinia striiformis ultrastructure slow-rusting resistance

Identification of transporter proteins for PQQ-secretion pathways by transcriptomics and proteomics analysis in Gluconobacter oxydans WSH-003

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Frontiers of Chemical Science and Engineering 2017年第1期11卷 72-88页

作者： Hui Wan Yu Xia Jianghua Li zhen kang Jingwen Zhou School of Biotechnology and Key Laboratory of Industrial B iotechnology Ministry of Education Jiangnan University Wuxi 214122 China Synergetic Innovation of Center of Food Safety and Nutrition Jiangnan University Wuxi 214122 China

Pyrroloquinoline quinone （PQQ） plays a sig- nificant role as a redox cofactor in combination with dehydrogenases in bacteria. These dehydrogenases play key roles in the oxidation of important substrates for the biotechnology industry, such as vitamin C production. While biosynthesis of PQQ genes has been widely studied, PQQ-transport mechanisms used both two-dimensional remain unclear. Herein, we fluorescence-difference gel electrophoresis tandem mass spectrometry and RNA sequencing to investigate the effects ofpqqB overexpres- sion in an industrial strain of Gluconobacter oxydans WSH-003. We have identified 73 differentially expressed proteins and 99 differentially expressed genes, a majority of which are related to oxidation-reduction and transport processes by gene ontology analysis. We also described several putative candidate effectors that responded to increased PQQ levels resulting from pqqB overexpression. Furthermore, quantitative PCR was used to verify five putative PQQ-transport genes among different PQQ producing strains, and the results showed that ompW, B932 1930 and B932_2186 were upregulated in all conditions. Then the three genes were over-expressed in G. oxydans WSH-003 and PQQ production were detected. The results showed that extracellular PQQ of B932_1930 （a transporter） and B932_2186 （an ABC transporter perrnease） overexpression strains were enhanced by 1.77- fold and 1.67-fold, respectively. The results suggest that the proteins encoded by PqqB, B932_1930 and B932 2186 might enhance the PQQ secretion process.

关键词： 2D-DIGE pqqB pyrroloquinoline quinone,RNA-Seq Vitamin C

Engineering Escherichia coli for high-yield production of ectoine

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Green Chemical Engineering 2023年第2期4卷 217-223页

作者： Daoan Wang Jiamin Chen Yang Wang Guocheng Du zhen kang The Science Center for Future Foods Jiangnan UniversityWuxi214122China The Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationJiangnan UniversityWuxi214122China The Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi214122China

Ectoine is a natural macromolecule protector and synthesized by some *** provides protections against radiation-mediated oxidative damages and is widely used as a bioactive ingredient in pharmaceutics and *** meet its growing commercial demands,we engineered Escherichia coli strains for the high-yield production of *** ectABC gene cluster from the native ectoine producer Halomonas elongata was intro-duced into different Escherichia coli(***)strains via plasmids and 0.8 g L^(-1)of ectoine was produced in flask cultures by engineered *** BL21(DE3).Subsequently,we designed the ribosome-binding sites of the gene cluster to fine-tune the expressions of genes ectA,ectB,and ectC,which increased the ectoine yield to 1.6 g L^(-1).After further combinatorial overexpression of Corynebacterium glutamicum aspartate kinase mutant(G1A,C932T)and the *** aspartate-semialdehyde dehydrogenase to increase the supply of the precursor,the titer of ectoine reached to 5.5 g L^(-1)in flask ***,the engineered strain produced 60.7 g L^(-1)ectoine in fed-batch cultures with a conversion rate of 0.25 g/g glucose.

关键词： Ectoine Escherichia coli Metabolic engineering RBS design

Effcient transesterifcation resolution of 2-(4-chlorophenyl)-4-hydroxytetrahydropyran with magnetically separable immobilized lipase

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Chinese Chemical Letters 2013年第12期24卷 1112-1114页

作者： Ping Xue zhen-zhen kang Xiao-Yong Lai Guan-Qun Qu Yuan-Yuan Li State Key Laboratory Cultivation Base of Natural Gas Conversion Ningxia University

We report an efficient kinetic resolution of racemic 2-（4-chlorophenyl）-4-hydroxytetrahydro-pyran （CLP-4-HTHP） via Pseudomonas cepacia lipase （PSL）-catalyzed transesterification, where PSL is immobilized on a core-shell MnFe204@SiO2-（CH2）3-NH2 carrier and used as a magnetically separable catalyst. The as-synthesized PSL/MnFe204@SiO2-（CH2）3-NH2 catalyst exhibits enhanced catalytic activity for resolving racemic CLP-4-HTHP to the corresponding optically pure （2R,4S）-CLP-4-HTHP compared to the free PSL. The ees for the former is 2.3 times larger than that for the latter under optimized conditions （99.4% and 44.1%, respectively）, although the eep for them are same （99.2%）. Meanwhile, the PSL/MnFe204@SiO2-（CH2）3-NH2 catalyst possesses a high saturate magnetization of 59.7 emu/g and could be easily recovered by magnetic separation and reused. The catalytic activity in six recycling tests did not significantly decrease, suggesting its great potential for industrial applications.

关键词： Optically pure 2-(4-chlorophenyl)-4- hydroxytetrahydropyran Magnetically immobilized lipase Enanatioselectivity Asymmetric transesterification

Comparative analysis of protein kinases and associated domains between Ascomycota and Basidiomycota

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Journal of Integrative Agriculture 2019年第1期18卷 96-107页

作者： PEI Guo-liang GUO Jun WANG Qin-hu kang zhen-sheng State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant ProtectionNorthwest A&F University

Protein kinases play an important role in every aspect of cellular *** this study,we systemically identified protein kinases from the predicted proteomes of 59 representative fungi from Ascomycota and *** analysis revealed that fungi from Ascomycota and Basidiomycota differed in the number and variety of protein *** groups of protein kinases,such as calmodulin/calcium regulated kinases(CMGC) and those with the highest group percentages are the most prevalent protein kinases among all fungal species *** contrast,the STE group(homologs of the yeast STE7,STE11 and STE20 genes),was more abundant in Basidiomycetes than in ***,the distribution of some protein kinase families appeared to be *** tyrosine kinase-like(TKL) group had a higher protein kinase density in Agaricomycotina *** addition,the distribution of accessory domains,which could have functional implications,demonstrated that usage bias varied between the two *** component analysis revealed a divergence between the main functional domains and associated domains in *** study provides novel insights into the variety and expansion of fungal protein kinases between Ascomycota and Basidiomycota.

关键词： protein kinases associated domains Ascomycota Basidiomycota

Identification of three Berberis species as potential alternate hosts for Puccinia striiformis f. sp. tritici in wheat-growing regions of Xinjiang, China

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Journal of Integrative Agriculture 2019年第12期18卷 2786-2792页

作者： ZHUANG Hua ZHAO Jing HUANG Li-li kang zhen-sheng ZHAO Jie State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant Protection Northwest A&F University

Since the recent discovery of barberry(Berberis spp.)as an alternate host for the stripe rust pathogen Puccinia striiformis,many Chinese Berberis species have been identified as alternate hosts for *** ***,little is known about Berberis species and their distribution in wheat-growing regions in Xinjiang,China,where stripe rust is *** the largest province or autonomous region,Xinjiang represents a relatively independent epidemic region for wheat stripe rust in *** this study,we con ducted a survey of barberry plants in the main wheat-growi ng areas of *** iden tified three Berberis species,***,*** and ***,and con firmed their roles as potential alternate hosts for *** *** in the laboratory.

关键词： barberry alternate host sexual reproduction stripe rust yellow rust

Regularization dependence of pion generalized parton distributions

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Chinese Physics C 2022年第6期46卷 66-87页

作者： Jin-Li Zhang Guang-zhen kang Jia-Lun Ping Department of Physics Nanjing Normal UniversityNanjing 210023China School of Science Yangzhou Polytechnic InstituteYangzhou 225127China

Pion generalized parton distributions are calculated within the framework of the Nambu-Jona-Lasinio model using different regularization schemes,including the proper time regularization scheme,the three-dimensional(3D)momentum cutoff scheme,the four-dimensional momentum cutoff scheme,and the Pauli-Villars regularization ***,we check the theoretical constraints of pion generalized parton distributions required by the symmetries of quantum chromodynamics in different regularization *** diagrams of pion parton distribution functions are plotted,in addition,we evaluate the Mellin moments of generalized parton distributions,which are related to the electromagnetic and gravitational form factors of *** generalized parton distributions are continuous but not differential at x=±ξ,when considering the effect of the contact contribution term,generalized parton distributions become not continuous at x=±ξin all the four regularization *** parton distributions in impact parameter space are considered,the width distribution of u quark in the pion and the meansquaredπu are *** light-front transverse-spin distributions are studied when quark polarized in the light-front-transverse+x direction,the transverse-spin density is no longer symmetric around(bx=0,by=0),the peaks shift to(bx=0,by>0),we compare the average transverse shift1u and2u in different regularization *** light-cone energy radius rE,LC and the light-cone charge radius rc,LC are also evaluated,we found that in the proper time regularization scheme the values of these quantities were the largest,in the 3 D momentum cutoff scheme they were the smallest.

关键词： generalized parton distributions form factors parton distribution functions

Synthesis and Crystal Structure of 4-(4,6-dimethoxyl -pyrimidin-2-yl)-3-thiourea Carboxylic Acid Methyl Ester

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Chinese Journal of Structural Chemistry 2006年第2期25卷 168-172页

作者： HUANG Jie SONG Ji-Rong REN Ying-Hui XU kang-zhen MA Hai-Xia Department of Chemical Engineering Shanxi Key Laboratory of Physico-inorganic Chemistry Northwest Univers＇ity Xi＇an 710069 China

The title compound 4-（4,6-dimethoxylpyrimidin-2-yl）-3-thiourea carboxylic acid methyl ester was synthesized by the reaction of 2-amino-4,6-dimethoxyl pyrimidine, potassium thiocyanate and methyl chloroformate in ethyl acetate. Single crystals suitable for X-ray measurement were obtained by recrystallization with the solvent of dimethyl formamide at the room temperature. The structure was characterized by elemental analysis and IR and determined by X-ray diffraction analysis＇ Crystallographic data： C9H12N4O4S, Mr = 272.29, monoclinic, space group C2/m with a = 1.6672（3）, b = 0.66383（12）, c = 1.1617（2） nm, β = 109.275（2）°, V = 1.2136（4） nm^3, Dc = 1.490 g/cm^3,μ = 0.281 mm^-1, F（000） = 568, Z = 4, R1 = 0.0341and wR2 = 0.1042.

关键词： 4-(4,6-dimethoxylpyrimidin-2-yl)-3-thiourea carboxylic acid methyl ester synthesis X-ray diffraction

Gene knockdown by structure defined single-stem loop small non-coding RNAs with programmable regulatory activities

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Synthetic and Systems Biotechnology 2023年第1期8卷 86-96页

作者： Yang Wang Guobin Yin Huanjiao Weng Luyao Zhang Guocheng Du Jian Chen zhen kang The Science Center for Future Foods Jiangnan UniversityWuxi214122China The Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi214122China The Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationJiangnan UniversityWuxiChina

Gene regulation by trans-acting small RNAs(sRNAs)has considerable advantages over other gene regulation ***,synthetic sRNAs mainly take natural sRNAs(MicC or SgrS)as backbones and comprise three functional elements folding into two or more stem-loop structures:an mRNA base pairing region,an Hfq-binding structure,and a rho-independent *** to limited numbers of natural sRNAs and complicated backbone structures,synthetic sRNAs suffer from low activity programmability and poor structural ***,natural sRNA backbone sequences may increase the possibility of unwanted ***,we present a bottom-up approach for creating structure defined single-stem loop small non-coding RNAs(ssl-sRNAs),which contain a standardized scaffold of a 7 bp-stem-4 nt-loop-polyU-tail and a 24 nt basing pairing region covering the first eight ***,ssl-sRNA requires no independent Hfq-binding structure,as the polyU tail fulfills the roles of binding Hfq.A thermodynamic-based scoring model and a web server sslRNAD(http://***/)were developed for automated design of ssl-sRNAs with well-defined structures and programmable ***-sRNAs displayed weak polar effects when regulating polycistronic *** ssl-sRNA designed by sslRNAD showed regulatory activities in both Escherichia coli and Bacillus subtilis.A streamlined workflow was developed for the construction of customized ssl-sRNA and ssl-sRNA *** examples,the *** cell morphology was easily modified and new target genes of ergothioneine biosynthesis were quickly identified with ***-sRNA and its designer sslRNAD enable researchers to rapidly design sRNAs for knocking down target genes.

关键词： Regulatory RNA Metabolic engineering Synthetic biology De novo design Ergothioneine

Synthesis and Crystal Structure of 4-(4,6-Dimethoxylpyrimidin-2-yl)-3-thiourea Carboxylic Acid Ethyl Ester

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Chinese Journal of Structural Chemistry 2008年第2期27卷 195-199页

作者： ZHANG Yang HUANG Jie SONG Ji-Rong REN Ying-Hui XU kang-zhen Department of Chemical Engineering Shaanxi Key Laboratory of Physico-inorganic Chemistry Northwest University Xi＇an 710069 China Conservation Technology Department the Palace Museum4 Jingshan Qianjie Beijing 100009 China

4-（4,6-Dimethoxyl-pyrimidin-2-yl）-3-thiourea carboxylic acid ethyl ester was synthesized by the reaction of 2-amino-4,6-dimethoxyl pyrimidine, potassium thiocyanate and methyl chloroformate in ethyl acetate. Single crystals suitable for X-ray measurement were obtained by recrystallization with the solvent of dimethyl formamide at room temperature. The crystal structure was determined by X-ray diffraction analysis. Crystallographic data： C10H14N4O4S, M, = 286.31, monoclinic, space group C2/c with a = 2.5309（3）, b = 0.67682（6）, c = 1.74237（19） nm, β = 114.744（3）°, V= 2.7106（5） nm3, Dc = 1.403 g/cm3, p = 0.225 mm-1, F（000） = 1200, Z= 8, R= 0.0514 and wR= 0.1529.

关键词： 4-(4,6-dimethoxyl-pyrimidin-2-yl)-3-thiourea carboxylic acid ethyl ester,synthesis crystal structure, ^-~ aromatic stacking interactions