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Cross-talk between microRNA-let7c and transforming growth factor-β2 during epithelial-to-mesenchymal transition of retinal pigment epithelial cells

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International Journal of Ophthalmology(English edition) 2020年第5期13卷 693-700页

作者： qu-zhen Deji Feng Yan Wang-Dui Zhaba Ya-Jun Liu Jie Yin zhen-Ping Huang Department of Ophthalmology Affiliated Jinling HospitalMedical School of Nanjing UniversityNanjing 210002Jiangsu ProvinceChina Department of Neurosurgery Affiliated Jinling HospitalMedical School of Nanjing UniversityNanjing 210002Jiangsu ProvinceChina Department of Ophthalmology Affiliated Drum Tower HospitalMedical School of Nanjing UniversityNanjing 210008Jiangsu ProvinceChina

AIM: To explore the roles of microRNA-let7 c(miR-let7 c) and transforming growth factor-β2(TGF-β2) and cellular signaling during epithelial-to-mesenchymal transition(EMT) of retinal pigment epithelial cells. METHODS: Retinal pigment epithelial(ARPE-19) cells were cultured with no serum for 12 h, and then with recombinant human TGF-β2 for different lengths of time. ARPE-19 cells were transfected with 1×106 TU/mL miR-let7 c mimcs(miR-let7 cM), miR-let7 c mimcs negative control(miR-let7cMNC) and miR-let7 c inhibitor(miR-let7 cI) using the transfection reagent. The expression of keratin-18, vimentin, N-cadherin, IKB alpha, p65 were detected by Western blot, quantitative polymerase chain reaction and immunofluorescence. RESULTS: The expression of miR-let7c was dramatically reduced and the nuclear factor-kappa B(NF-κB) signaling pathway was activated after induction by TGF-β2(P<0.05). In turn, overexpressed miR-let7 c significantly inhibited TGF-β2-induced EMT(P<0.05). However, miR-let7 c was unable to inhibit TGF-β2-induced EMT when the NF-κB signaling pathway was inhibited by BAY11-7082(P<0.01). CONCLUSION: The miR-let7 c regulates TGF-β2-induced EMT through the NF-κB signaling pathway in ARPE-19 cells.

关键词： microRNA-let7c transforming growth factor-β2 epithelial-to-mesenchymal transition human retinal pigment epithelial cells nuclear factor-kappa B pathway

Upreguiation of human telomerase reverse transcriptase mRNA expression by in vitro transfection of hepatitis B virus X gene into human hepatocarcinoma and cholangiocarcinoma cells

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World Journal of Gastroenterology 2005年第36期11卷 5627-5632页

作者： zhen-Liang qu Sheng-quan Zou Nai-Qiang Cui Xian-Zhong Wu Ming-Fang Qin Di Kong zhen-Li Zhou Department of surgery Tianjin Nankai Hospital Tianjin 300100 China Department of Surgery Tongji Hospital Wuhan430030 Hubei Province China

AIM： To study the changes of human telomerase reverse transcriptase （hTERT） mRNA expression in human hepatocarcinoma cell lines （HepG2） and cholangiocarcinoma cell lines （QBC939） after HBx gene transfection and to illustrate the significance of transcriptional regulation of hTERT gene by HBx gene in the carcinogenesis. METHODS： HepG2 and QBC939 cell lines were cultured and co-transfected with eukaryotic expression vector containing the HBx coding region and cloning vector containing enhanced green fluorescent protein （EGFP） coding sequence using lipid-mediated gene transduction technique. Thirty-six hours after transfection, EGFP expression in cells was used as the indicator of successful transfection. Flow cytometry was performed to determine the transfection efficiency. Cells were harvested and total RNA was extracted using TRIzol reagent. The expression of hTERT mRNA in HepG2 and QBC939 cell lines was assayed by reverse transcriptionpolymerase chain reaction. The expression of HBx protein in both cell lines was detected by immunocytochemical staining and Western blotting. RESULTS： Flow cytometry showed that the transfection efficiency was 46.4% in HepG2 cells and 29.6% in QBC939 cells for both HBx gene expression vector and blank vector. The expression of hTERT mRNA was meaningfully increased in HepG2 and QBC939 cell lines when transfected with HBx gene expression vector compared to those transfected with OPTI-MEM medium and blank vector. Immunocytochemical staining and Western blotting revealed HBx protein expression in HepG2 and QBC939 cells only when transfected with HBx gene. CONCLUSION： HBx gene transfection can upregulate the transcriptional expression of hTERT mRNA. The transactivation of hTERT gene by HBx gene is a newfound mechanism for pathogenesis of hepatocarcinomas and cholangiocarcinomas after HBV infection. 2005 The WJG Press and Elsevier Inc. All rights reserved

关键词： Hepatocholangiocarcinoma Human telomerasereverse transcriptase Gene expression Hepatitis B virus X protein

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智能化验机器人控制指令网络传输优化系统

电子设计工程 2024年第18期32卷 150-153,158页

作者：王洪晖李元明梁银海曲震陕西延长石油富县发电有限公司陕西延安727502 南京国电环保科技有限公司江苏南京210061

海量控制指令在传输过程中容易引起网络传输时延,为避免该情况的发生,设计智能化验机器人控制指令网络传输优化系统。硬件部分以微控制器为基础,通过RS-232串口控制指令网络传输时延;设计控制指令网络传输电路,通过SPI接口输出数据;使... 详细信息

海量控制指令在传输过程中容易引起网络传输时延,为避免该情况的发生,设计智能化验机器人控制指令网络传输优化系统。硬件部分以微控制器为基础,通过RS-232串口控制指令网络传输时延;设计控制指令网络传输电路,通过SPI接口输出数据;使用请求/应答传输结构,获取控制指令序列。软件方面设计数据传输控制时序获取响应信号;引入正交匹配追踪算法,计算经过稀疏重构的信号输出结果;结合系统端对端时钟同步算法,实现控制指令网络传输优化。由实验结果可知,该系统最大平均吞吐量为560 b/s,最快传输速率为110 kbps,具有较优的传输效果。

关键词：智能化验机器人控制指令网络传输传输时延正交匹配追踪算法

Durotomy and dural grafting to treat lower cervical spine injuries with extensive spinal cord edema

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Neural Regeneration Research 2015年第12期10卷 1969-1970页

作者： Yan-zhen qu Xiao-dong Guo Department of Orthopedics Union HospitalTongji Medical CollegeHuazhong University of Science and Technology

Lower cervical spine injuries not only cause cervical fracture,dislocation,and facet interlocking,but also lead to spinal cord *** damage caused by extensive spinal cord edema after spinal cord injury(SCI)can signific... 详细信息

关键词： SCS Durotomy and dural grafting to treat lower cervical spine injuries with extensive spinal cord edema than SCI

The Role of Neck Evolution in the Synthesis of Superheavy Element 112

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Chinese Physics Letters 2013年第8期30卷 42-45页

作者： ZHU Min FU Jun-Li qu zhen LIU Zu-Hua WANG Wen-Zhong School of Science Minzu University of ChinaBeijing 100081 Key Laboratory of Beam Technology and Material Modification of Ministry of Education Beijing Normal UniversityBeijing 100875 China Institute of Atomic Energy Beijing 102413

The coupled and uncoupled Langevin equations in two-dimensional collective space are used to study the neck dynamics of the mass asymmetric system ^(48)Ca+^(238)U at the above barrier *** results show that the coupling between the neck and radial degrees of freedom delays the transition from dinucleus to mononucleus,and correspondingly increases the lifetime of the dinucleus *** lifetime of the dinucleus for the asymmetric system^(48)Ca+^(238)U is about 11.6 and 13.0×10^(-22) s,obtained with uncoupled and coupled Langevin *** calculate the evaporation residue(ER)cross sections for the 3n and 4n evaporation channels in the ^(48)Ca+^(238)U reaction leading to the formation of ^(283)112 and ^(282)112 isotopes in three different approaches,i.e.,coupled,uncoupled and frozen approximation,and compare them with the experimental *** is found that the results of the uncoupled and frozen approximation are in close similarity,while the coupling between the radial and neck degrees of freedom reduces the ER cross section by about 30%,compared with the case of frozen approximation.

关键词： lifetime Langevin radial

基于护士主导的集束化抗凝管理在华法林抗凝治疗房颤患者中的应用价值

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四川生理科学杂志 2024年第2期46卷 288-290,413页

作者：杜欢欢翟景明屈振威邱立帅河南科技大学第一附属医院心外科河南洛阳471000 河南科技大学第一附属医院胃肠外科河南洛阳471000 河南科技大学第一附属医院外科ICU 河南洛阳471000 河南科技大学第一附属医院手术室河南洛阳471000

目的:探讨基于护士主导的集束化抗凝管理模式对房颤患者华法林抗凝治疗国际标准化比值(International normalized ratio,INR)达标率及血液流变学水平的影响。方法:选取我科2021年3月至2022年9月期间112例房颤拟服用或正在服用华法林的... 详细信息

目的:探讨基于护士主导的集束化抗凝管理模式对房颤患者华法林抗凝治疗国际标准化比值(International normalized ratio,INR)达标率及血液流变学水平的影响。方法:选取我科2021年3月至2022年9月期间112例房颤拟服用或正在服用华法林的患者作为研究对象,按随机数字法分为两组,常规组56例用药期间行常规护理指导,抗凝组56例给予护士主导的集束化抗凝管理模式,对两组患者进行为期3 m的干预,采集静脉血对比抗凝效果和血液流变学指标,通过调查问卷对比自我管理能力,统计并记录干预期间并发症发生率。结果:抗凝组INR达标率低于常规组,TTR值高于常规组(P0.05),干预3 m后,两组AHSMSRS各维度评分均升高,且抗凝组高于常规组(P0.05),干预3 m后,两组D-D、NB、FIB、FDP水平均降低,且抗凝组低于常规组(P<0.05);抗凝组抗凝并发症发生率低于常规组(P<0.05)。结论:基于护士主导的集束化抗凝管理模式应用于房颤患者华法林抗凝治疗有助于增强抗凝效果,改善血液流变学,增强自我管理能力,降低抗凝并发症发生率。

关键词：房颤华法林集束化抗凝管理国际标准化比值

A scalable one-pot strategy for the development of polymer electrolytes adaptable to room-temperature high-voltage lithium batteries

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Chinese Chemical Letters 2024年第3期35卷 461-467页

作者： Yue Guo Xinxin qu zhen Li Ruiyuan Tian Xiaokong Liu State Key Laboratory of Supramolecular Structure and Materials College of ChemistryJilin UniversityChangchun 130012China Key Laboratory of Physics and Technology for Advanced Batteries(Ministry of Education) College of PhysicsJilin UniversityChangchun 130012China

Poly(ethylene oxide)(PEO)polymer electrolytes(PEs)have been commercially applied in LiFePO_(4)||Li solid-state lithium batteries(SSLBs).However,it remains challenging to develop PEO-based PEs applicable to the high-voltage SSLBs with higher energy density,owing to the poor electrochemical stability of ***,we report a scalable strategy for fabricating PEO-based PEs with high-voltage compatibility,by exploiting a new mechanism to stabilize the cathode-electrolyte interface in the highvoltage *** protocol only involves a one-pot synthesis procedure to covalently crosslink the PEO chains,in the presence of high-content lithium bis(trifluoromethylsulphonyl)imide(LiTFSI)salts and N,N-dimethylformamide(DMF).LiTFSI-DMF supramolecular aggregates are formed and firmly embedded in the polymer network,endowing the PE with high room-temperature ionic *** dissociated and highly concentrated TFSI^-anions can enter the Helmholtz layer close to the high-voltage cathode,leading to the formation of a thin and homogeneous cathode electrolyte interface(CEI),mainly composed of LiF,on the *** CEI with high electrochemical stability can effectively stabilize the cathode-electrolyte interface,enabling long-term stable cycling of the high-voltage LiCoO_(2)||Li and nickelrich NCM_(622)||Li batteries at room *** simplicity and scalability of the strategy makes the reported PEO-based PE potentially applicable in high-voltage SSLBs in practice.

关键词： Lithium batteries Polymer electrolytes Poly(ethylene oxide) Energy density High-voltage cathodes

Enhancing flotation recovery of residual carbon from gasification waste by mixing hydrophobic powder with diesel as collector

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Particuology 2024年第6期89卷 211-217页

作者： Rui Han Ningning Zhang Anning Zhou zhen Li Jinzhou qu Hong Wang College of Chemistry and Chemical Engineering Xi’an University of Science and TechnologyXi’anShaanxi 710054China

Coal gasification fine slag(CGFS)is a solid waste containing residual carbon and ash generated during the coal gasification process,and the separation of the two components is the essential way to realize its environmental pollution reduction and resource value *** flotation is the preferred method for separating CGFS,but there is a barrier of low carbon recovery in this process due to the extensive adsorption of collector by the well-developed pores on residual *** this study,a sufficiently simple yet innovative collector,a mixture of hydrophobic powder and diesel,was proposed in an attempt to break the *** experiments with common diesel and this novel collector were performed respectively,and FTIR,XPS,and SEM-EDX were employed to analyze the collector action *** results revealed that the novel collector could significantly improve the residual carbon recovery;test results demonstrated that the novel collector could increase the hydrophobic functional group content on the fine slag surface,and the hydrophobic powders in this novel collector mainly appeared at the pore openings of the flotation *** essence of the mechanism is that the hydrophobic powders play a dual role of blocking pores and providing adsorption sites,thus facilitating the spreading of diesel on the carbon surface and promoting its *** study can provide creative ideas for the efficient disposal of coal gasification waste.

关键词： Coal gasification fine slag Carbon recovery Collector Fotation Hydrophobic powder

西藏地区血液净化质量控制单位单中心维持性血液透析患者血管通路现况分析

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中国血液净化 2024年第6期23卷 453-456页

作者：索朗曲珍阿勇李国梁崔专唐雯西藏自治区人民医院肾内科拉萨850000 北京大学第三医院肾内科北京100191

目的调查西藏地区血液净化质量控制单位血管通路现况。方法以2021年12月─2022年12月在西藏自治区人民医院血液净化中心进行维持性血液透析(maintenance hemodialysis,MHD)的患者为研究对象。结果共纳入194例患者,使用临时静脉置管... 详细信息

目的调查西藏地区血液净化质量控制单位血管通路现况。方法以2021年12月─2022年12月在西藏自治区人民医院血液净化中心进行维持性血液透析(maintenance hemodialysis,MHD)的患者为研究对象。结果共纳入194例患者,使用临时静脉置管、带隧道和涤纶套导管(tunnel-cuffed catheter,TCC)、移植物动静脉内瘘(arteriovenous graft,AVG)和自体动静脉内瘘(arteriovenous fistula,AVF)作为首次血管通路的比例分别为34.0%、45.4%、1.0%和19.6%。24.2%患者在透析前完成造瘘。提前造瘘和未提前造瘘的患者比,男性(χ^(2)=3.919,P=0.048)和大学及以上学历(χ^(2)=17.875,P<0.001)更多;和未提前造瘘组相比,提前造瘘组患者透析后以AVF或AVG为血管通路的比例更高(χ^(2)=4.860,P=0.029)。在MHD患者中,以AVF、AVG、TCC为血管通路的比例分别为86.6%、2.6%、10.8%。和使用AVF或AVG的患者相比,使用TCC为血管通路的患者年龄更大(t=2.039,P=0.043),原发性肾小球肾炎更少(χ^(2)=4.213,P=0.040)。透析开始血管通路为临时中心静脉导管的患者(n=66)和其它血管通路患者(n=128)相比,有更高的比例以TCC作为MHD血管通路(χ^(2)=8.157,P=0.007)。结论 AVF使用率符合指南要求,TCC使用率略高于指南要求,需要技术提高和加强教育。

关键词：血管通路动静脉内瘘血液透析