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Expression and Identification of Mutated Osteoprotegerin in Culture Cells and Larvae of Silkworm

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Acta Biochimica et Biophysica Sinica 2004年第5期36卷 331-335页

作者： Hai-Long XIAO, Yong-Feng JIN, and Yao-Zhou ZHANG1* Institute of Biochemistry, college of Life Sciences, zhejiang university, Hangzhou 310029, China college of Life Sciences, zhejiang university of Science & Technology, Hangzhou 310018, China college of Life Sciences Zhejiang University of Science & TechnologyHangzhou 310018China Institute of Biochemistry College of Life SciencesZhejiang UniversityHangzhou 310029China

The mutated osteoprotegerin (OPG-372) gene was inserted into the baculovirus transfer vector pBacPAK8, and the recombinant plasmid was co-transfected with linearized Bm-BacPAK6 virus DNA into BmN cells, then homologous recombination occurred inside the cells. The recombinant virus BmNPV-OPG- 372 was screened and identified by Southern blotting. The recombinant human OPG-372 was expressed in cultured cells and the larvae of silkworm by inoculation of recombinant virus. The expression products were run on the SDS-PAGE and their immunoreactivities were determined by Western blotting. It was found that a 42 kD recombinant protein was expressed in BmN cells and a 46 kD one in larvae respectively. The bioactivi- ties of the recombinant proteins were determined by hypocalcemic effect assay in the mice. The results showed that the recombinant proteins had a significant hypocalcemic effect on mice sera.

关键词： OPG-372 silkworm baculovirus expression system hypocalcemic effect

Accumulation of Pathogenesis-related Type-5 Like Proteins in Phytoplasma-infected Garland Chrysanthemum Chrysanthemum coronarium

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Acta Biochimica et Biophysica Sinica 2004年第11期36卷 773-779页

作者： Bo-Xiong ZHONG and Yan-Wei SHENDepartment of Bioresource Science, college of Animal Science, zhejiang university, Hangzhou 310029, China Department of Bioresource Science College of Animal Science Zhejiang University Hangzhou 310029 Chin

Soluble proteins extracted from leaves, apical shoots, axillary shoots, and stems of garland chrysanthemum plants infected by onion yellows phytoplasma were analyzed by two-dimensional gel electrophoresis. Computerized matching analysis revealed that at least six soluble proteins were accumulated specifically in phytoplasma-infected garland chrysanthemum. N-terminal amino acids sequences of these soluble

关键词： phytoplasma garland chrysanthemum Chrysanthemum coronarium pathogenesis-related proteins thaumatin osmotin

New multisoliton solutions of the(2+1)-dimensional dispersive long wave equations

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Communications in Nonlinear Science and Numerical Simulation 2001年第3期6卷 178-182页

作者： Jiefang ZHANG and Ping HAN Institute of Nonlinear Physics, zhejiang Normal university, Jinhua 321004, China college of Engineering, zhejiang Ocean university, Zhoushan 316004, China Institute of Nonlinear Physics Zhejiang Normal University Jinhua 321004 China college of Engineering Zhejiang Ocean University Zhoushan 316004 China

Using the extension homogeneous balance method, we have obtained a nonlinear transformation to simplify the (2+1)-dimensional dispersive long wave equations into a linear partial differential equation, and found some ... 详细信息

关键词： multisoliton (2+1)-dimensions dispersive long wave equations

Influence of Particle Size on Magnetic Properties of Soils in zhejiang Province, China

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Pedosphere 2000年第1期10卷 81-88页

作者： LU SHENGGAO (college of Environment and Resource, zhejiang university, Hangzhou 510029 (China)) 浙江大学浙江杭州 310029

The relationship between magnetic properties and particle size of soils derived from metamorphic rock, basalt, granite, Quaternary red clay, limestone and mudstone from zhejiang Province, East China was studied. Based on the variations of the mass magnetic susceptibility (X), anhysteretic remanent magnetization (ARM), and saturation isothermal remanent magnetization (SIRM) with soil particle size, the relationship could be classified into three groups. For the soils derived from metamorphic rock and basalt, magnetic values were the highest in the gravel and coarse sand fractions and decreased with decreasing soil particle size. The soils derived from sedimentary rock had a bimodal distribution of magnetic values, with peaks in 1-0.5 and 0.005-0.000 5 mm fractions. The soil developed on granite was characterized by a peak of magnetic value in 0.001-0.000 5 mm fractions. Frequency-dependent susceptibility (Xfd ) and ratics of magnetic parameters (ARM/X, SIRM/X and SIRM/ARM) of soil particle fractions showed that variations in ferrimagnetic grain size paralleled those in particle size. Xfd peaked in clay fraction and decreased with increasing particle size, irrespective of soil parent materials. The acquisition curves of IRM and demagnetization parameter of different soil particles indicated that there were different magnetic minerals assemblages in different particle fractions.

关键词： soil particle soil parent material magnetic property zhejiang Province

Supramolecularly assembled water layers stabilized by sebacic anions in complexes of Zn(II) and Co(II)

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Science China Chemistry 2010年第12期53卷 2539-2546页

作者： XIAN HuiDuo,LIU JianFeng & ZHAO GuoLiang zhejiang Key Laboratory for Reactive Chemistry on Solid Surfaces Institute of Physical Chemistry,zhejiang Normal university college of Chemistry and Life Science,zhejiang Normal university,Jinhua 321004,China 1. zhejiang Key Laboratory for Reactive Chemistry on Solid Surfaces Institute of Physical Chemistry Zhejiang Normal University Jinhua 321004 China2. College of Chemistry and Life Science Zhejiang Normal University Jinhua 321004 China

Two three-dimensional supramolecular water architectures,[Zn(phen)3]2 ·[Zn(C10H16O4)·(H2O)3]·(C10H16O4)2·20H2O(1) and [Co(phen)3]2 ·[Co(H2O)6]·(C10H16O4)3·30H2O(2)[phen=1,10-Phenanthroline,C10H16O4=sebacic dianion],have been synthesized and characterized by IR,elemental analysis,thermogravimetric analysis,and single-crystal X-ray *** two structures both contain extensive hydrogen bonding between water molecules as well as between water molecules and sebacic *** water molecules and sebacic acid O atoms assembled 2D supramolecular corrugated sheets with different morphology in the two complexes.

关键词： Zn(II) Co(II) complex sebacic acid crystal structure supramolecular self-assemble water cluster

Simultaneous detection of seven phenolic acids in Danshen injection using HPLC with ultraviolet detector

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Journal of zhejiang university-Science B(Biomedicine & Biotechnology) 2008年第9期9卷 728-733页

作者： Jin-zhong XU, Jie SHEN, Yi-yu CHENG, Hai-bin QU (Department of Chinese medicine Science and Engineering, college of Pharmaceutical Sciences, zhejiang university, Hangzhou 310058, China) Department of Chinese medicine Science and Engineering College of Pharmaceutical Sciences Zhejiang University Hangzhou 310058 China.

A high-performance liquid chromatographic (HPLC) method with ultraviolet (UV) detector had been developed for simultaneous quantification of danshensu, protocatechuic aldehyde, caffeic acid, salvianolic acid D, rosmarinic acid, salvianolic acid B and salvianolic acid A in Danshen injection. According to the UV spectra of these components, three detection wavelengths have been selected as follows: 280 nm for danshensu and protocatechuic aldehyde, 326 nm for caffeic acid, salvianolic acid D and rosmarinic acid, 286 nm for salvianolic acid B and salvianolic acid A. The limit of detection (LOD) was improved to be in the range of 0.008~0.160 μg/ml. Moreover, excellent linear behavior over the investigated concentration range was observed, with R>0.999 for all the analytes.

关键词： High-performance liquid chromatography (HPLC) Danshen injection (DSI) Phenolic acids Multi-wavelengths Quantification

Immobilized chitosan as a selective absorbent for the nickel removal in water sample

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Journal of Environmental Sciences 2003年第5期15卷 633-638页

作者： WU Jian min, WANG Yong yao (Department of Chemistry, college of Science, zhejiang university, Hangzhou 310027, China. ) Department of Chemistry College of Science Zhejiang University Hangzhou 310027 Chin

Method for preparation of chitosan immobilized on silica gel(CTS silica) was described. The CTS silica was used as absorbent for the absorption of nickel in water. The results showed that this absorbent had relatively high selectivity and strong affinity to nickel. The maximum absorption capacity for nickel can reach 667 mg/g of chitosan. Factors that affect the absorption capacity, such as pH, ion strength and the presence of calcium, EDTA and the mechanism of absorption were discussed in detail. The absorbent can be regenerated with acid and reused for several times. The recovery rate for nickel can reach 99 99%. This absorbent filled in a column can be used in nickel removal from wastewater and drinking water.

关键词： immobilized chitosan nickel wastewater treatment

Effect of lead on survival, locomotion and sperm morphology of Asian earthworm, Pheretima guillelmi

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Journal of Environmental Sciences 2009年第5期21卷 691-695页

作者： ZHENG Rongquan, LI Canyang college of Chemistry and Life Science, zhejiang Normal university, Jinhua 321004, China. [a]college of Chemistry and Life Science Zhejiang Normal University Jinhua 321004 China

To provide basic toxicity data for formulating risk characterization benchmarks, the effects of lead on survival, locomotion, and sperm morphology were investigated in the Asian earthworm Pheretima guillelmi. The LC50 of P. guiUelmi for 7 and 14 d were 4285＋339 mg/kg and 3207＋248 mg/kg, which shows P. guillelmi can tolerate a higher concentration of lead nitrate. The average weight of the surviving earthworms decreased at concentration of 2800 mg Pb/kg soil, and the locomotor ability of earthworms exposed to a range of soil Pb concentrations showed a general decrease with increasing Pb concentrations. We also presented data depicting the sperm morphology of earthworms, which shows potential as a sensitive biomarker for measuring the effects of heavy metal on reproduction.

关键词： lead Pheretima guillelmi sperm morphology locomotion

Influences of Quinclorac on Culturable Microorganisms and Soil Respiration in Flooded Paddy Soil

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Biomedical and Environmental Sciences 2003年第4期16卷 314-322页

作者： ZHEN-MEI LU, HANG MIN , AND YANG-FANG YE Institute of Microbiology, college of Life Science, zhejiang university, Hangzhou 310029, zhejiang, China Institute of Microbiology College of Life Science Zhejiang University Hangzhou 310029 Zhejiang China

Objective To investigate the potential effects of herbicide quinclorac (3,7-dichloro-8-quinoline-carboxylic) on the culturable microorganisms in flooded paddy soil. Methods Total soil aerobic bacteria, actinomycetes and fungi were counted by a 10-fold serial dilution plate technique. Numbers of anaerobic fermentative bacteria (AFB), denitrifying bacteria (DNB) and hydrogen-producing acetogenic bacteria (HPAB) were numerated by three-tube anaerobic most-probable-number (MPN) methods with anaerobic liquid enrichment media. The number of methanogenic bacteria (MB) and nitrogen-fixing bacteria (NFB) was determined by the rolling tube method in triplicate. Soil respiration was monitored by a 102G-type gas chromatography with a stainless steel column filled with GDX-104 and a thermal conductivity detector. Results Quinclorac concentration was an important factor affecting the populations of various culturable microorganisms. There were some significant differences in the aerobic heterotrophic bacteria. AFB and DNB between soils were supplemented with quinclorac and non-quinclorac at the early stage of incubation, but none of them was persistent. The number of fungi and DNB was increased in soil samples treated by lower than 1.33μg·g-1 dried soil, while the CFU of fungi and HPAB was inhibited in soil samples treated by higher than 1.33μg·g-1 dried soil. The population of actinomycete declined in negative proportion to the concentrations of quinclorac applied after 4 days. However, application of quinclorac greatly stimulated the growth of AFB and NFB. MB was more sensitive to quinclorac than the others, and the three soil samples with concentrations higher than 1 μg·g-1 dried soil declined significantly to less than 40% of that in the control, but the number of samples with lower concentrations of quinclorac was nearly equal to that in the control at the end of experiments. Conclusion Quinclorac is safe to the soil microorganisms when applied at normal concentrations (0.67

关键词： Quinclorac Soil culturable microorganisms Soil respiration Flooded paddy soil