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“Heaven and Man Integrating each other” Or “Man and Nature Being in Harmony”——and Discussion of “Different Times for Different Emphases”

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学术界 2010年第7期 269-270页

作者： Wang Xinjian Zhang Qipeng Colloge of Maxism,zhejiang university of science and technology

Over the years,in academic circles,there is hardly any unified view in the study of the ancient philosophical proposition "Heaven and man integrating each other",whose original connotation of broad topic indeed is a strong foundation for scholars of Chinese philosophy and abided by the love of the content,which is also beyond *** "adherence" can not lead to fundamentalism."Heaven," meaning the modern transformation of the old proposition that is "due to be focused along the times," the interpretation of this,how can scholars miss or neglect?

关键词： Man and Nature Being in Harmony Or Different Times for Different Emphases Heaven and Man Integrating each other and Discussion of Nature

THREE-SPHERE INEQUALITIES FOR SECOND ORDER SINGULAR PARTIAL DIFFERENTIAL EQUATIONS

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Acta Mathematica Scientia 2010年第3期30卷 993-1003页

作者：张松艳 zhejiang university of science and technology

In this article, we give the three-sphere inequalities and three-ball inequalities for the singular elliptic equation div（A∨u） - Vu =0, and the three-ball inequalities on the characteristic plane and the three-cylinder inequalities for the singular parabolic equation Эtu-div（A∨u）＋ Vu = 0, where the singular potential V belonging to the Kato-Fefferman- Phong＇s class. Some applications are also discussed.

关键词： Three-sphere inequality three-cylinder inequality singular partial differential equation Kato-Fefferman-Phong's class Lipschitz domain

Generative computer simulation for parallel layout of multi-story row house cluster

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science China(Technological sciences) 2010年第7期53卷 1785-1791页

作者： DING XiYing1,2 1 Zhenjianguniversity, Hangzhou 310058, China 2 zhejiang university of science & technology, Hangzhou 310058, China 1. Zhenjiang university Hangzhou 310058 China2. Zhejiang University of Science & Technology Hangzhou 310058 China

This study has established the functions between the environmental conditions and the inhabitants’ preferences for multi-story row house cluster with parallel layout, based on the data from the questionnaires’ in Beijing. A program has been written with multi-agent system and generative computer simulation approaches. The emergence candidate layout plans can be referenced and chosen by architects.

关键词： multi-agent system bottom up generative computer simulation multistory row house cluster parallel layout

Expression and Identification of Mutated Osteoprotegerin in Culture Cells and Larvae of Silkworm

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Acta Biochimica et Biophysica Sinica 2004年第5期36卷 331-335页

作者： Hai-Long XIAO, Yong-Feng JIN, and Yao-Zhou ZHANG1* Institute of Biochemistry, College of Life sciences, zhejiang university, Hangzhou 310029, China College of Life sciences, zhejiang university of science & technology, Hangzhou 310018, China College of Life sciences Zhejiang University of Science & TechnologyHangzhou 310018China Institute of Biochemistry College of Life SciencesZhejiang UniversityHangzhou 310029China

The mutated osteoprotegerin (OPG-372) gene was inserted into the baculovirus transfer vector pBacPAK8, and the recombinant plasmid was co-transfected with linearized Bm-BacPAK6 virus DNA into BmN cells, then homologous recombination occurred inside the cells. The recombinant virus BmNPV-OPG- 372 was screened and identified by Southern blotting. The recombinant human OPG-372 was expressed in cultured cells and the larvae of silkworm by inoculation of recombinant virus. The expression products were run on the SDS-PAGE and their immunoreactivities were determined by Western blotting. It was found that a 42 kD recombinant protein was expressed in BmN cells and a 46 kD one in larvae respectively. The bioactivi- ties of the recombinant proteins were determined by hypocalcemic effect assay in the mice. The results showed that the recombinant proteins had a significant hypocalcemic effect on mice sera.

关键词： OPG-372 silkworm baculovirus expression system hypocalcemic effect

Simultaneous Determination of Five Components Including Acetaminophen by Reversed-phase High Performance Liquid Chromatography

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Journal of Wuhan university of technology(Materials science) 2004年第1期19卷 34-35页

作者：张立庆 zhejiang university of science and technology

High performance liquid chromatography with a C18 reverse-phase column was used to separate the five components in cough syrup,including acetaminophen,p-aminophenol,caffeine,chlorphenamine maleate and *** mobile phase consists of 15wt% acetonitrile,0.004mol/L sodium heptyl sulfonate, 0.03mole/L potassium di-hydrogen phosphate and triethylamine (volume ratio 13∶40∶44∶3),the pH of which is adjusted to 3.0 by phosphoric *** contents of the five components are analyzed on an ultraviolet spectrophotometer at 254nm,with a flow rate of 0.4mL/*** results show that the calibration curves are linear in a certain *** average recovery of five components is between 96.31% and 102.3%.

关键词： organic compounds preparation and determination chromatography

Simultaneous Spectrophotometric Determination of Four Components including Acetaminophen by Taget Factor Analysis

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Journal of Wuhan university of technology(Materials science) 2007年第3期22卷 569-571页

作者：张立庆 Shool of Biological and Chemical Engineering Zhejiang University of Science and Technology

UV Spectrophotometric Target Factor Analysis （TFA） was used for the simultaneous determination of four components （acetaminophen, guuaifenesin, caffeine, Chlorphenamine maleate） in cough syrup. The computer Frogram of TFA is based on VC＋＋ language. The difficulty of overlapping of absorption spectra of four compounds was overcome by this procedure. The experimental results show that the average recovery of each component is all in the range from 98.9% to 106.8% and each component obtains satisfactory results without any pre-separation.

关键词： acetaminophen taget factor analysis spectrophotometry four-component simultaneous determination

pH-Dependent Stability of EGX, A Multi-functional Cellulase from Mollusca, Ampullaria crossean

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Acta Biochimica et Biophysica Sinica 2004年第9期36卷 603-608页

作者： Wen-Ying LI2, Ji WANG1, Yan-Hong LI1, Ming DING1, Gen-Jun XU1,3, Lan-Ying LIU2, and Fu-Kun ZHAO1,3* 1Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological sciences, the Chinese Academy of sciences,Graduate School of the Chinese Academy of sciences, Shanghai 200031, China 2College of Life science, Jilin university, Changchun 130023, China College of Life science, zhejiang university of science and technology, Hangzhou 310018, China Key Laboratory ofProteomics Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological Sciencesthe Chinese Academy of SciencesGraduate School of the Chinese Academy of SciencesShanghai 200031China

The cellulase activity and stability of EGX, a multi-functional cellulase previously purified from the mollusca Ampullaria crossean, was systematically studied under different pH. The pH induced con- formation and stability change of EGX have been investigated by using the intrinsic fluorescence, ANS fluorescence and CD spectrum. It has been found that the conformation and activity of this cellulase were strongly dependent on the pH. EGX was stable for both the enzyme activity and the conformation from pH 5.6 to pH 7.4. As shown by intrinsic and ANS fluorescence, no red shift of emission maximum occurred and a negligible intensity change was observed at pH 5.6–7.4. The activity of EGX remained about 80% in pH 5.6–7.4 and obviously decreased out of side the pH range. Urea-induced changes in EGX at pH 5.4 and pH 8.0 were measured by intrinsic fluorescence and CD spectrum. At pH 5.4, a significantly red shift of emission maximum occurred when the concentration of urea was 5 M compared to the concentration was 3 M at pH 8.0. The α-helix at pH 5.4 was 40.51% in the absence of urea and 31.04% in the presence of 4 M urea. At pH 8.0 the α-helix was 7.23% in the presence of 4 M urea. The data indicated that EGX was much susceptible to urea-induced unfolding at pH 8.0 and much stable at pH 5.4. The greater pH dependent stability of EGX may allow the enzyme to adequately catalyze the hydrolysis of cellulosic materials under natural or industrial extreme conditions.

关键词： multi-functional cellulase stability Ampullaria crossean fluorescence spectroscopy pH-dependent structural change CD spectrum

Analysis and Countermeasure for Latch-up of CMOS Device in Electronic System Design

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微计算机信息 2008年第5期24卷 -页

作者： WANG XINHUA WANG JIANFEN(zhejiang university of science and technology,Department of information and electronic engineering,Hangzhou 310023. China)

Considering the phenomenon of Latch-up in CMOS device,some patterns of manifestation about Latch-up in electronic system are analyzed with three actual circuits,meanwhile the correlative solving methods are also provided. To avoid Latch-up,some general principles are proposed. The analyzing and solving processes derived from practical system design are verified simple and ef-fective in large number of products,and to some extend have general reference value in anti-latch-up design of application systems.

关键词： Latch-up trig signal integrality dual-powered system

An efficient method for processing a large-scale motion database in agriculture informatisation

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An efficient method for processing a large-scale motion data...