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Effects of cisplatin on telomerase activity and telomere length in BEL-7404 human hepatoma cells

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Cell Research 2002年第1期12卷 55-62页

作者： Ru GANG ZHANG, Ru PING ZHANG, XING WANG WANG, HONG XIE Department of Biotherapy, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological sciences, the Chinese Academy of sciences, Shanghai 200031, China 1Department of Biotherapy Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200031 China 1Department of Biotherapy Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200031 China 1Department of Biotherapy Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200031 China 1Department of Biotherapy Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200031 China

Telomerase activity was inhibited in a dose and time-dependent manner with the treatment of cisplatin for 24, 48, or 72 h in a concentration ranged from 0.8 to 50 1uM in BEL-7404 human hepatoma cells. There were no changes in expression pattern of three telomerase subunits, its catalytic reverse transcriptase subunit (hTERT), its RNA component (hTR) or the associated protein subunit (TP1), after cisplatin treated for 72 h with indicated concentrations. Mean telomere lengths were decreased by the cisplatin treatment. Cell growth inhibition and cell cycle accumulation in G2/M phase were found to be correlated with telomerase inhibition in the present study, but percentages of cell apoptosis did not change markedly during the process.

关键词： Telomerase telomere cisplatin hepatocellular carcinoma cell cycle cell growth.

GROUND STATES OF A NONLINEAR ELLIPTICSYSTEM IN R^N

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Acta Mathematica Scientia 1997年第3期17卷 283-300页

作者：杨健夫 CHINESE ACAD SCI WUHAN INST PHYS & MATHYOUNG SCIENTIST LAB MATH PHYSWUHAN 430072PEOPLES R CHINA NANCHANG UNIV DEPT MATHNANCNANG 330047PEOPLES R CHINA

We investigate existence of ground states of nonlinear elliptic systems in R-N and establish decaying laws for solutions of the systems at infinity.

关键词： ground state nonlinear elliptic system decaying law

Predicting Grain Yield and Protein Content in Winter Wheat at Different N Supply Levels Using Canopy Reflectance Spectra

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Pedosphere 2007年第5期17卷 646-653页

作者： XUE Li-Hong CAO Wei-Xing YANG Lin-Zhang Key Laboratory of Crop Growth Regulation Ministry of Agriculture Nanjing Agricultural University Nanjing 210095 （China） Institute of Soil sciences Chinese Academy of Sciences Nanjing 210008 （China）

A field experiment using a split-plot randomized complete block design with three replications was carried out to determine relationships between spectral indices and wheat grain yield （GY）, to compare the performance of four vegetation indices （VIs） for GY prediction, and to study the feasibility of VI to estimate grain protein content （GPC） in winter wheat. Two typical winter wheat （Triticum aestivum L.） cultivars ＇Xuzhou 26＇（high protein content） and ＇Huaimai 18＇（low protein content） were used as the main plot treatments and four N rates, i.e., 0, 120, 210, and 300 kg N ha^-1, as the sub-plot treatments. Increasing soil N supply significantly increased GY and GPC （P ≤ 0.05）. For the two cultivars combined, significant and positive correlations were found between four VIs and GY, with the strongest relationship observed when using the green ratio vegetation index （GRVI） at mid-filling. Cumulative VI estimates improved yield predictions substantially, with the best interval being heading to maturity stage. Similar results were found between VI and grain protein yield. However, when using cumulative VI, GPC showed no significant improvement. The strong relationship between leaf N status and GPC （R2 =0.9144 for ＇Xuzhou 26＇ and R2 = 0.8285 for ＇Huaimai 18＇） indicated that canopy spectra could be used to predict GPC. The strong fit between estimated and observed GPC （R2 = 0.7939） indicated that remote sensing techniques were potentially useful predictors of grain protein content and quality in wheat.

关键词： grain protein content grain yield soil N supply vegetation index wheat

Pollen morphology of the family Smilacaceae and its phylogenetic and taxonomic significance

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Pollen morphology of the family Smilacaceae and its phylogen...

Quantum phase transitions of fermionic atoms in an anisotropic triangular optical lattice

浙江省第二届生物多样性保护与可持续发展研讨会

作者： SHI-CHAO CHEN, CHEN-XIN FU , XIAO-PING ZHANG , SHI-FENG NI and KENNETH M. CAMERON Laboratory of Systematic and Evolutionary Botany & Biodiversity, College of Life sciences, zhejiang university, Hangzhou, zhejiang, P. R. China. College of Life sciences. Anhui Normal university, Wuhu, Anhui. P. R. China. The New York Botanical Garden, Bronx, NY, USA

The pollen morphology of 125 species belonging to 3 genera of the Smilacaceae was investigated with light and scanning electron microscopy, of which 10 species were also examined by transmission electron microscopy, in order to find additional taxonomic informations for phylogenetic analysis of the family. The variations of pollen morphology in Smilacaceae are sufficient to permit two pollen classes and four pollen types to be distinguished. The pollen grains of all Smilax and Heterosmilax species are spheroidal, inaperturate and spinulate or microspinulate, with thin and fragile exine of varied sculperturing, which is remarkably distinct from Ripogonum that are prolate, monosulcate, and reticulate. Ripogonum was recognized as a separate pollen class and type of its own, whereas Smilax and Heterosmilax were grouped into another pollen class with three pollen types. It can be concluded that Ripogonum should be separated as a single family as its pollen type is incompatible with those in most of species of Smilacaceae. Heterosmilax can be incorprated into the Smilax based on the fact that the successive variation series of pollen morphology occur in these two genera. A key for the identification of these pollen types as well as the possible phylogenetic trends of pollen types are presented.

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Chinese Physics B 2013年第11期22卷 216-221页

作者：保安陈耀桦章晓中 Laboratory of Advanced Materials School of Materials Science and Engineering Tsinghua University Beijing National Laboratory for Condensed Matter Physics Institute of Physics Chinese Academy of Sciences

The effect of anisotropy caused by a confining potential on the properties of fermionic cold atoms in a triangular optical lattice is systematically investigated by using the dynamical cluster approximation combined with the continuous time quantum Monte-Carlo algorithm. The quantum phase diagrams which reflect the temperature-interaction relation and the competition between the anisotropic parameter and the interaction are presented with full consideration of the anisotropy of the system. Our results show that the system undergoes a transition from Fermi liquid to Mott insulator when the repulsive interaction reaches a critical value. The Kondo effect also can be observed in this system and the pseudogap is suppressed at low temperatures due to the Kondo effect. A feasible experiment protocol to observe these phenomena in an anisotropic triangular optical lattice with cold atoms is proposed, in which the hopping terms are closely related to the lattice confining potential and the atomic interaction can be adjusted via the Feshbach resonance.

关键词： triangular optical lattice cold atoms cluster dynamical approximation

土霉素的电化学还原和吸附溶出伏安法测定研究

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分析化学 1989年第12期17卷 1072-1076页

作者：韦桂珍陆宗鹏 AlanM.Bond 中国科技大学中国科学院生态环境中心澳大利亚Deakin大学合肥 230026 100083 Vic.3217

本文叙述吸附溶出分析土霉素的灵敏方法。土霉素及其质子化形态能在汞电极表面吸附富集（-0.60V vsAg/AgCl）,溶出峰的数目及电位受pH影响。在pH4—8的溶液中得到4个溶出峰,而在强酸性和碱性介质中仅得到一个主峰。文中讨论了有关的化... 详细信息

本文叙述吸附溶出分析土霉素的灵敏方法。土霉素及其质子化形态能在汞电极表面吸附富集（-0.60V vsAg/AgCl）,溶出峰的数目及电位受pH影响。在pH4—8的溶液中得到4个溶出峰,而在强酸性和碱性介质中仅得到一个主峰。文中讨论了有关的化学和电极反应机理。采用奥氏方波溶出,电解富集5 min的测定限为5×10-10mol/L。

关键词：吸附溶出伏安法土霉素

Organic Phosphorus in Shallow Lake Sediments in Middle and Lower Reaches of the Yangtze River Area in China

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Pedosphere 2008年第3期18卷 394-400页

作者： JIN Xiang-Can WANG Sheng-Rui CHU Jian-Zhou WU Feng-Chang State Environmental Protection Key Laboratory for Lake Pollution Control Research Center of Lake Eco-EnvironmentChinese Research Academy of Environmental Sciences Beijing 100012 （China） Colleges of Resource and Environment Hebei Agriculture University Baoding 071001 （China） State Key Laboratory of Environmental Geochemistry Institute of Geochemistry Chinese Academy of Sciences Guiyang550002 （China）

Thirteen sediment core samples(0-10 cm) were taken from the seven lakes in the middle and lower reaches of the Yangtze River to determine the contents and distributions of organic phosphorus(P) fractions in the sediments of the shallow lakes in the *** organic P fractions in the sediments were in the order of moderately labile organic P(MLOP) > moderately resistant organic P(MROP) > highly resistant organic P(HROP) > labile organic P(LOP),with average proportional ratios of 13.2:2.8:1.3:***,MLOP,and MROP were significantly related to the contents of total organic carbon(TOC),water-soluble P(WSP),algal-available P(AAP),NaHCO3-extractable P(Olsen-P),total P(TP),organic P(OP),and inorganic P(IP).However,HROP was significantly related to OP and weakly correlated with TOC,WSP,AAP,Olsen-P,TP or *** suggested that organic P,especially LOP and MLOP in sediments,deserved even greater attention than IP in regards to lake *** terms of organic P,sediments were more hazardous than soils in lake *** OP concentrations were higher in moderately polluted sediment than those in heavily polluted sediment,LOP and MLOP were higher in the heavily polluted sediment,which indicated that heavily polluted sediment was more hazardous than moderately polluted sediment in lake eutrophication.

关键词： bioavailability chemical extracted phosphorus lake sediment middle and lower reaches of the Yangtze River area organic phosphorus fractions

A statistical study of the relation between soft X-ray excess and accretion disk

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Science China(Physics,Mechanics & Astronomy) 2010年第S1期53卷 102-105页

作者： LIU JieYing1,2,3 & QIAO ErLin1,2 1 National Astronomical Observatories/Yunnan Observatory,Chinese Academy of Science,Kunming 650011,China 2 Key Laboratory for the Structure and Evolution of Celestial Objects,Chinese Academy of sciences,Kunming 650011,China 3 Graduate university of Chinese Academy of sciences,Beijing 100049,China 1. National Astronomical Observatories/Yunnan Observatory Chinese Academy of Science Kunming 650011 China 2. Key Laboratory for the Structure and Evolution of Celestial Objects Chinese Academy of Sciences Kunming 650011 China 3. Graduate university of Chinese Academy of sciences Beijing 100049 China

To study the origin of the soft X-ray excess,we compile a sample of 94 unobscured,radio-quiet QSOs and Seyfert galaxies with available data from GALEX and *** find that 50 sources show strong soft X-ray excess and the other 44 show weak/no soft X-ray *** analyses of the data indicate that the difference in soft X-rays is mainly but not only resulting from different accretion rates(in units of Eddington rate).The statistical study of the sources with soft X-ray excess shows that the strength of soft X-ray excess weakly and positively correlates with the Eddington ratio and increases with the increase of the strength of UV radiations relative to the *** that the UV emissions are from the thin disk,the correlations imply that the origin of soft X-ray excess is associated with the thin disk,either by means of Comptonization of the disk photons or in some other ways.

关键词： accretion:accretion disks ultraviolet:galaxies X-rays:galaxies

Electric Field Effects on Animals:Mechanism of Seismic Anomalous Animal Behaviors (SAABs)

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Earthquake Research in China 1997年第1期11卷 107-116页

作者： Huang Qinghua,Ikeya Motoji,and Huang PeihuaDepartment of Earth and Space sciences,Graduate School of Science,Osaka university,Osaka 560,Japan Department of Earth and Space sciences,university of Science and Technology of China,Hefei 230026,China

Electric field effect on animals has been studied to investigate its relation with seismic anomalous animal behaviors(SAABs)in *** eel,crucian carp,catfish,and soft-shelled turtle responded to the threshold electric field of 1-10 V/m,while duck,goose,cat,sheep,pig,dog,and chicken all responded to the ground electric field of about tens of V/m,depending on the species as well as on *** of the behaviors caused by electric field were similar to those reported as SAABs such as alignment,sudden movement,panic,and *** intensity of electric field due to a major earthquake would have been over these threshold *** estimation based on an electromagnetic model of a fault has been made to induce SAABs as electric shocks to pulsed electric fields in *** seismic electric signals(SES)intensity might be estimated from the observation of SAABs.

关键词： : Seismic anomalous behaviors of animals (SAABs) Seismic electric signals (SES) Electromagnetism Piezoelectric effect Free charge