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Small RNAs in pollen

Science China(Life Sciences) 2015年第3期58卷 246-252页

作者： HE Hui YANG TianYu WU WenYe zheng binglian State Key Laboratory of Genetic Engineering Collaborative Innovation Center for Genetics and Development Institute of Plant BiologySchool of Life Sciences Fudan University

In plants, each pollen mother cell undergoes two rounds of cell divisions to form a mature pollen grain, which contains a vegetative cell(VC) and two sperm cells(SC). As a companion cell, the VC carries the SCs to an ovule by germinating a pollen tube. In-depth sequencing analyses of mature pollen showed that micro RNAs(mi RNAs) and short interfering RNAs(si RNAs) are present in both the VC and SCs. Additionally, epigenetically-regulated transposable elements(TEs) are reactivated in the VC and these TE m RNAs are further processed into 21-nt epigenetically reactivated si RNA(easiR NA) in SCs, which prevent 24-nt si RNA accumulation and sequester mi RNA loading. Small RNAs are thought to move from the VC to SCs, where they regulate gene expression and reinforce TE silencing. Here, we summarize current knowledge of the biogenesis and function of mi RNAs, si RNAs, and easi RNAs in pollen, emphasizing how these different small RNAs coordinately contribute to sperm cell formation and TE silencing.

关键词： vegetative cell sperm cell easi RNA transposable elements

A reciprocal inhibition between ARID1 and MET1 in male and female gametes in Arabidopsis

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Journal of Integrative Plant Biology 2017年第9期59卷 657-668页

作者： Lei Li Wenye Wu Youshang Zhao binglian zheng State Key Laboratory of Genetic Engineering Collaborative Innovation Center for Genetics and DevelopmentSchool of Life SciencesFudanUniversityShanghai 200438China

Both female and male gametophytes harbor companion cells and gametes. MET1, a DNA methyltransferase, is down-regulated in companion cells. However, how MET1 is differentially regulated in gametophytes remains unexplored. ARID1, a transcription factor that is specifically depleted in sperm cells, is occupied by MET1- dependent CG methylation. Here, we show that MET1 confines ARID1 to the vegetative cell of male gametes, but ARID1 conversely represses MET1 in the central cell of female gametes. Compared to the vegetative celllocalization in wild type pollen, ARID1 expands to sperm cells in the met1 mutant. To understand whether MET1- dependent ARID1 inhibition exists during female gametogenesis, we first show that ARID1 is expressed in the megaspore mother cell （MMC）, ARID1 but not MET1 is detectable in the central cell at maturity. Interestingly, compared to the absence of MET1 in the central cell and the egg cell of wild type ovules, MET1 significantly accumulates in these two cells in aridl ovules. Lastly, we show that both ARIDI and METI are required for the cell specification of MMC. Collectively, our results uncover a reciprocal dependence between ARIDI and METI, and provide a clue to further understand how the specification of MMC is likely regulated by DNA methylation.

关键词： Figure YFP A reciprocal inhibition between ARID1 and MET1 in male and female gametes in Arabidopsis

Brassinosteroids inhibit miRNA-mediated translational repression by decreasing AGO1 on the endoplasmic reticulum

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Journal of Integrative Plant Biology 2021年第8期63卷 1475-1490页

作者： Taiyun Wang Yanhua zheng Qi Tang Songxiao Zhong Wei Su binglian zheng State Key Laboratory of Genetic Engineering Ministry of Education Key Laboratory of Biodiversity Sciences and Ecological EngineeringCollaborative Innovation Center of Genetics and DevelopmentInstitute of Plant BiologySchool of Life SciencesFudan UniversityShanghai200438 China

Translational repression is a conserved mechanism in microRNA(miRNA)-guided gene *** Arabidopsis,ARGONAUTE1(AGO1),the major miRNA effector,localizes in the cytoplasm for mRNA cleavage and at the endoplasmic reticulum(ER)for translational repression of target ***,the mechanism underlying miRNA-mediated translational repression is poorly *** particular,how the subcellular partitioning of AGO1 is regulated is largely ***,we show that the plant hormone brassinosteroids(BRs)inhibit miRNA-mediated translational repression by negatively regulating the distribution of AGO1 at the ER in Arabidopsis *** show that the protein levels rather than the transcript levels of miRNA target genes were reduced in BR-deficient mutants but increased under BR *** localization of AGO1 at the ER was significantly decreased under BR treatments while it was increased in the BR-deficient ***,ROTUNDIFOLIA3(ROT3),an enzyme involved in BR biosynthesis,co-localizes with AGO1 at the ER and interacts with AGO1 in a GW motif-dependent *** analysis showed that the AGO1-ROT3 interaction is necessary for the function of *** findings provide new clues to understand how miRNA-mediated gene silencing is regulated by plant endogenous hormones.

关键词： AG01 Arabidopsis thaliana brassinosteroids endoplasmic reticulum miRNA translational repression

A lariat-derived circular RNA is required for plant development in Arabidopsis

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Science China(Life Sciences) 2018年第2期61卷 204-213页

作者： Jinping Cheng Yong Zhang Ziwei Li Taiyun Wang Xiaotuo Zhang binglian zheng State Key Laboratory of Genetic Engineering Collaborative Innovation Center for Genetics and Development School of Life SciencesFudan University

Lariat RNA is produced during pre-mRNA splicing, and it is traditionally thought as by-products, due to the quick turnover by debranching followed by degradation. However, recent findings identified many lariat RNAs accumulate with a circular form in higher eukaryotes. Although the remarkable accumulation, biological consequence of lariat-derived circular RNAs(here we name laciRNAs) remains largely unknown. Here, we report that a specific laciRNA from At5 g37720 plays an essential role in plant development by regulating gene expression globally. We focus on 17 laciRNAs with accumulation in wild type plants by circular RNA sequencing in Arabidopsis. To determine biological functions of these laciRNAs, we constructed one pair of transgenic plants for each laciRNA, in which the local gene with or without introns was over-expressed in wild type plants,respectively. By comparing morphological phenotypes and transcriptomic profiles between two classes of transgenic plants, we show that over-expression of the laciRNA derived from the 1 st intron of At5 g37720 causes pleiotropic phenotypes, including curly and clustered leaf, late flowering, reduced fertility, and accompanied with altered expression of approximately 800 *** results provide another example that a specific plant circular RNA regulates gene expression in a similar manner to that of other non-coding RNAs under physiological conditions.

关键词： lariat RNA circular RNA intron flowering time pleiotropic phenotype

Cajal Bodies Are Developmentally Regulated during Pollen Development and Pollen Tube Growth in Arabidopsis thaliana

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Molecular Plant 2013年第4期6卷 1355-1357页

作者： Regina Scarpin Lorena Sigaut Lia Pietrasanta Sheila McCormick binglian zheng Jorge Muschietti Instituto de Ingeniería Genética y Biología Molecular (INGEBI-CONICET) Vuelta de Obligado 2490 Piso 2 Buenos Aires 1428 Argentina Centro de Microscopías Avanzadas Universidad de Buenos Aires 1428 Buenos Aires Argentina Plant Gene Expression Center US Department of Agriculture/Agricultural Research Service and Department of Plant and Microbial Biology University of California at Berkeley Albany CA 94710 USA State Key Laboratory of Genetic Engineering and Institute of Plant Biology School of Life Sciences Fudan University Shanghai 200433 China Departamento de Biodiversidad y Biología Experimental Facultad de Ciencias Exactas y Naturales Universidad de Buenos AiresBuenos Aires 1428 Argentina

Dear Editor, Cajal bodies （CBs） are sub-nuclear bodies first described in neurons by Ramon y Cajal in 1903 and subsequently character- ized in both plant and animals （Gall, 2003）. They appear close to the nucleoli as discrete foci whose size and number vary during the cell cycle （Strzelecka et al., 2010）. They are dynamic structures that move, fuse, and separate, depending on the transcriptional status of the cell （Cioce and Lamond, 2005）. CBs are involved in assembly and trafficking of small nucleolar ribonucleopro- teins （snoRNPs） and in assembly and trafficking of spliceoso- mal small nuclear ribonucleoprotein （snRNPs） complexes that are involved in mRNA splicing （Gall, 2003; Cioce and Lamond, 2005）. CBs also contain components of the machinery involved in siRNA-mediated silencing and in methylation of repetitive DNA （Li et al., 2006）.

关键词： Developmentally Regulated Arabidopsis

The chromatin remodeling complex imitation of switch controls stamen filament elongation by promoting jasmonic acid biosynthesis in Arabidopsis

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Journal of Genetics and Genomics 2021年第2期48卷 123-133页

作者： Youshang Zhao Ting Jiang Lei Li Xiaotuo Zhang Tianyu Yang Cuimei Liu Jinfang Chu binglian zheng State Key Laboratory of Genetic Engineering Ministry of Education Key Laboratory of Biodiversity Sciences and Ecological EngineeringInstitute of Plant BiologySchool of Life SciencesFudan UniversityShanghai 200438China National Centre for Plant Gene Research(Beijing) Innovation Academy for Seed DesignInstitute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing 100039China

Plant reproduction requires the coordinated development of both male and female reproductive *** acid(JA)plays an essential role in stamen filament ***,the mechanism by which the JA biosynthesis genes are regulated to promote stamen elongation remains ***,we show that the chromatin remodeling complex Imitation of Switch(ISWI)promotes stamen filament elongation by regulating JA *** show that AT-Rich Interacting Domain 5(ARID5)interacts with CHR11,CHR17,and RLT1,several known subunits of *** in ARID5 and RLTs caused a reduced seed set due to greatly shortened stamen ***-seq analyses reveal that the expression of key genes responsible for JA biosynthesis is significantly down-regulated in the arid5 and rlt ***,the JA levels are drastically decreased in both arid5 and rlt *** immunoprecipitationquantitative PCR analyses further show that ARID5 is recruited to the chromatin of JA biosynthesis ***,exogenous JA treatments can fully rescue the defects of stamen filament elongation in both arid5 and rlt mutants,leading to the partial recovery of *** results provide a clue how JA biosynthesisis positively regulated by the chromatin remodeling complex ISWI,thereby promoting stamen filament elongation in Arabidopsis.

关键词： ARID Chromatin remodeling ISWI Stamen filaments Jasmonate biosynthesis

Cytokinin signal transduction:Known simplicity and unknown complexity

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Chinese Science Bulletin 2003年第13期48卷 1309-1315页

作者： zheng binglian, SUN Jiaqiang, ZHANG Suzhi, DENG Yan & ZUO Jianru Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China Correspondence should be addressed to Zuo Jianru (e-mail: jrzuo@ ***) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China

Cytokinin plays a critical role in plant growth and development by regulating cell divisions and cell differentiation. Recent studies suggest that cytokinin signaling is presumably mediated by a two-component system analogous to those found in bacteria and fungi, which transduces an external signal via a phosphorelay from the plasma membrane-anchored receptors to downstream effectors and regulators. Moreover, cytokinin signaling is highly interactive with other pathways, and many components of the pathway appear to be functionally redundant. Proper address of these questions will be crucial for our further understanding on this important network.

关键词：细胞分裂素信号转换植物生长基因突变

Nuclear body-localized ARID1 is required for male germline formation in Arabidopsis

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Nuclear body-localized ARID1 is required for male germline f...

Clearance of maternal barriers by paternal miR159 to initiate endosperm nuclear division in Arabidopsis

第十四届全国植物基因组学大会

作者： binglian zheng Yanhua zheng Hui He Ziwei Li Sheila McCormick State Key Laboratory of Genetic Engineering and Institute of Plant Biology School of Life SciencesFudan University Plant Gene Expression Center USDA/ARS and Dept.of Plant and Microbial Biology

ARID(AT-Rich Interacting Domain) proteins in animals are involved in cell cycle *** the ARID domain is conserved and widely present in plants,the function of plant ARID proteins has been largely *** role of ARID prote... 详细信息

关键词： ARID nuclear bodies epigenetic regulation male germline formation

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Clearance of maternal barriers by paternal miR159 to initiat...