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An integrated pipeline facilitates fast cloning of a new powdery mildew resistance gene from the wheat wild relative Aegilops umbellulata

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Plant Communications 2024年第11期5卷 10-13页

作者： Huagang He Jiale Wang Jiabao Liang Qianyuan Zhang Minfeng Xue zhaozhao chen Qiulian Tang Xiaobei chen Shanying Zhu Yajun Wang School of Life Sciences Jiangsu UniversityZhenjiangJiangsu 212013China Key Laboratory of Plant Design National Key Laboratory of Plant Molecular GeneticsCenter for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China Institute of Plant Protection and Soil Science Hubei Academy of Agricultural SciencesWuhan 430064China School of Environment and Safety Engineering Jiangsu UniversityZhenjiangJiangsu 212013China These authors contributed equally to this article

Dear Editor,Bread wheat(Triticum aestivum L.,2n=6x=42,AABBDD)is a major cereal crop consumed by approximately 30%of the global *** production faces continuous challenges from various fungal diseases,including powdery mildew,rusts,and Fusarium head blight(Singh et al.,2016).Aegilops umbellulata(***,2n=2x=14,UU),a diploid species within the genus Aegilops and closely related to cultivated bread wheat,exhibits resistance to several wheat pathogens,making it a valuable source of disease resistance genes for wheat ***,several resistance genes from *** have been successfully transferred into bread wheat,including the powdery mildew resistance gene PmY39(Zhu et al.,2006),the leaf rust resistance genes Lr9(Sears,1956)and Lr76(Bansal et al.,2020),and the stripe rust resistance gene Yr70(Bansal et al.,2020).Here,we investigate the responses of a panel of *** accessions to Blumeria graminis ***(Bgt),the pathogen causing wheat powdery mildew,and report the cloning of the powdery mildew resistance gene PmAeu1 through an integrated approach combining bulked segregant RNA sequencing(BSR-seq),fine genetic mapping,low-depth PacBio sequencing,and functional validation via stable transformation in wheat.

关键词： mildew Aegilops blight

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Molecular cytogenetic analyses of two new wheat-rye 6RL translocation lines with resistance to wheat powdery mildew

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The Crop Journal 2023年第2期11卷 584-592页

作者： Shanying Zhu Haonan Du Fuyu Su Jin Wang Qingfeng Meng Tianlei Liu Rui Guo zhaozhao chen Huanhuan Li Wenxuan Liu Pengtao Ma Huagang He School of Life Sciences Jiangsu UniversityZhenjiang 212013JiangsuChina School of Environment and Safety Engineering Jiangsu UniversityZhenjiang 212013JiangsuChina College of Life Sciences Yantai UniversityYantai 264005ShandongChina College of Life Sciences Henan Agricultural UniversityZhengzhou 450002HenanChina

Rye(Secale cereale)is a valuable gene donor for wheat improvement,especially for its resistance to *** rye-derived resistance sources is important for wheat *** the present study,two wheat-rye derivatives,designated JS016 and JS110,were produced by crossing common wheat cultivar Yangmai 23 with Pakistani rye accession *** sequential genomic in situ hybridization(GISH)and multicolor fluorescence in situ hybridization(mc-FISH),JS016 and JS110 were identified as a T6BS.6RL translocation line and a T6BS.6BL6RL translocation line,*** newly 6RL chromosome arm-specific markers were developed and used to confirm the 6RL *** wheat 55K single-nucleotide polymorphism(SNP)array further verified the molecular cytogenetic identification results above and clarified their breakpoints at 430.9 and 523.0 Mb of chromosome 6B in JS016 and JS110,*** spectrum and allelism test demonstrated that JS016 and JS110 possessed novel powdery mildew resistance gene(s)that was derived from the 6RL translocation but differed from ***,JS016 and JS110 had better agronomic traits than the previously reported 6RL translocation line carrying *** efficiently transfer and detect the 6RL translocation from JS016 and JS110,one 6RL-specific Kompetitive allele specific PCR(KASP)marker was developed and validated in high throughput marker-assisted selection(MAS).

关键词： Secale cereale Translocation line Wheat powdery mildew GISH/FISH KASP marker

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Orthologous genesPm12andPm21from twowild relatives of wheat show evolutionary conservation but divergent powdery mildew resistance

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Plant Communications 2023年第2期4卷 236-248页

作者： Shanying Zhu cheng Liu Shuangjun Gong zhaozhao chen Rong chen Tianlei Liu Renkang Liu Haonan Du Rui Guo Genying Li Miaomiao Li Renchun Fan Zhiyong Liu Qian-Hua Shen Anli Gao Pengtao Ma Huagang He School of Environment and Safety Engineering Jiangsu UniversityZhenjiang 212013China School of Life Sciences Jiangsu UniversityZhenjiang 212013China Crop Research Institution Shandong Academy of Agricultural SciencesJinan 250100China Institute of Plant Protection and Soil Science Hubei Academy of Agricultural SciencesWuhan 430064China School of Life Sciences Henan UniversityKaifeng 475004China State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyThe Innovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China College of Life Sciences Yantai UniversityYantai 264005China

Wheat powdery mildew,caused by Blumeria graminis ***(Bgt),is a devastating disease that threatens wheat production ***12,which originated from Aegilops speltoides,a wild relative of wheat,confers strong resistance to powdery mildew and therefore has potential use in wheat *** susceptible mutants induced by gamma irradiation,we physically mapped and isolated Pm12 and showed it to be orthologous to Pm21 from Dasypyrum villosum,also a wild relative of *** resistance function of Pm12 was validated via ethyl methanesulfonatemutagenesis,virus-induced gene silencing,and stable genetic *** analysis indicates that the Pm12/Pm21 loci in wheat species are relatively conserved but ***,we demonstrated that the two orthologous genes,Pm12 and Pm21,possess differential resistance against the same set of Bgt *** of the coiledcoil domains of both PM12 and PM21 induces cell death in Nicotiana benthamiana ***,their full-length forms display different cell death-inducing activities caused by their distinct intramolecular *** of Pm12 will facilitate its application in wheat breeding *** study also gives new insight into two orthologous resistance genes,Pm12 and Pm21,which show different race specificities and intramolecular interaction patterns.

关键词： Pm12 Pm21 powdery mildew resistance evolutionary conservation race specificity intramolecular interaction

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Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies

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Cancer Communications 2024年第3期44卷 408-432页

作者： Lu Tang Huan Zhang Fen Zhou Qiuzhe Wei Mengyi Du Jianghua Wu chenggong Li Wenjing Luo Jie Zhou Xindi Wang zhaozhao chen Yinqiang Zhang Zhongpei Huang Zhuolin Wu Yuxi Wen Huiwen Jiang Danying Liao Haiming Kou Wei Xiong Heng Mei Yu Hu Institute of Hematology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubeiP.R.China Hubei Clinical Medical Center of Cell Therapy For Neoplastic Disease WuhanHubeiP.R.China Key Laboratory of Biological Targeted Therapy the Ministry of EducationWuhanHubeiP.R.China Department of Thoracic Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubeiP.R.China Department of Pediatrics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubeiP.R.China Hubei Key Laboratory of Biological Targeted Therapy Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubeiP.R.China

Background:Chimeric antigen receptor T(CAR-T)therapy has substantially revolutionized the clinical outcomes of patients with hematologic malignan-cies,but the cancer-intrinsic mechanisms underlying resistance to CAR-T cells remain yet to be fully *** study aims to explore the molecular deter-minants of cancer cell sensitivity to CAR-T cell-mediated killing and to provide a better understanding of the underlying mechanisms and potential modulation to improve clinical ***:The human whole-genome CRISPR/Cas9-based knockout screening was conducted to identify key genes that enable cancer cells to evade CD19 CAR-T-cell-mediated *** in vitro cytotoxicity assays and evaluation of tumor tissue and bone marrow specimens were further conducted to confirm the role of the key genes in cancer cell susceptibility to CAR-T *** addition,the specific mechanisms influencing CAR-T cell-mediated cancer clearance were elucidated in mouse and cellular ***:The CRISPR/Cas9-based knockout screening showed that the enrich-ment of autophagy-related genes(ATG3,BECN1,and RB1CC1)provided protec-tion of cancer cells from CD19 CAR-T cell-mediated *** findings were further validated by in vitro cytotoxicity assays in cells with genetic and pharmacological inhibition of ***,higher expression of the three autophagy-related proteins in tumor samples was correlated with poorer respon-siveness and worse survival in patients with relapsed/refractory B-cell lymphoma after CD19 CAR-T *** RNA sequencing analysis of bone marrow samples from B-cell leukemia patients also suggested the clinical relevance of autophagy to the therapeutic response and relapse after CD19 CAR-T cell *** inhibition of autophagy and knockout of RB1CC1 could dramatically sensitize tumor cells to CD19 CAR-T cell-mediated killing in mouse models of both B-cell leukemia and ***,our study revealed that cancer-intrinsic

关键词： CAR-T resistance Autophagy Immune evasion Apoptosis Chemotaxis

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嵌合抗原受体T细胞在体监测与追踪研究进展

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临床血液学杂志 2022年第3期35卷 229-234页

作者：陈钊钊梅恒胡豫华中科技大学同济医学院附属协和医院血液病研究所武汉430022 湖北省肿瘤疾病细胞治疗临床医学研究中心

作为一种新兴的免疫治疗方式,嵌合抗原受体T细胞(chimeric antigen receptor T cells,CAR-T)疗法具有靶向性强和非MHC限制性的优势,该疗法是通过基因工程技术使T细胞表达靶向特异性肿瘤抗原的嵌合受体,从而杀伤特定肿瘤细胞^([1])。CAR-... 详细信息

作为一种新兴的免疫治疗方式,嵌合抗原受体T细胞(chimeric antigen receptor T cells,CAR-T)疗法具有靶向性强和非MHC限制性的优势,该疗法是通过基因工程技术使T细胞表达靶向特异性肿瘤抗原的嵌合受体,从而杀伤特定肿瘤细胞^([1])。CAR-T作为一种“活药”,其吸收、分布、代谢、排泄不符合传统药物的药代动力学。为进一步提高其疗效,需对CAR-T细胞的体内迁移、存活情况、生物分布、是否在靶及其数量.

关键词： CAR-T细胞细胞标记无创追踪动态监测

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贝特朗悖论新说

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赤峰学院学报（自然科学版） 2019年第11期35卷 22-25页

作者：陈召召陈城杨静安徽理工大学

先引入单位圆中点的分布实例,论述了原始三种方法的不足,给出两种新的求解方法,构建其求解模型,分别为"r类点"模型以及"θ弧弦"模型.文中对贝特朗问题,提出基于两种新模型的两种新解法,分别为点弦法和点点法.点弦法最终结果为0.60899778... 详细信息

先引入单位圆中点的分布实例,论述了原始三种方法的不足,给出两种新的求解方法,构建其求解模型,分别为"r类点"模型以及"θ弧弦"模型.文中对贝特朗问题,提出基于两种新模型的两种新解法,分别为点弦法和点点法.点弦法最终结果为0.6089977810.点点法最终结果为0.7468300049.文章的末尾,对点弦法与点点法做出相应的评价与分析.点弦法为直接以线构造弦方面做出的解释,而点点法则为以两点确立一条直线的原理来构造弦方面做出的解释.

关键词：贝特朗悖论概率论点弦法点点法

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