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Integration of chemoselective ligation with enzymespecific catalysis： Saccharic colorimetric analysis using aminooxy/hydrazine-functionalized gold nanoparticles

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Nano Research 2015年第12期8卷 3853-3863页

作者： Juan Zhang Jun Lv Xiaonan wang Defeng Li zhaoxia wang Genxi Li Laboratory of Biosensing Technology School of Life Sciences Shanghai University Shanghai 200444 China Shanghai Key Laboratory of Bio-Energy Crops Shanghai University Shanghai 200444 China Department of Oncology The Second Affiliated Hospital of Nanjing Medical University Nanjing 210011 China State Key Laboratory of Pharmaceutical Biotechnology Department of Biochemistry Nanjing University Nanjing 210093 China

Here we developed a saccharic colorimetric method based on the combination of chemoselective ligation and enzyme-specific catalysis using aminooxy/ hydrazine-functionalized gold nanoparticles （AO/AuNPs or H/AuNPs）. In the detection of galactose （Gal）, galactohexodialdose （GHDA）, the galactose oxidase （GalOx）-catalyzed product, has an aldehyde group, which allows it to chemoselectively react with an aminooxy or hydrazine group at the outer layer of AO/AuNPs or H/AuNPs by oxime/hydrazone click chemistry to form oxime or hydrozone. Consequent134 through the specific recognition of 1,4-phenylenediboronic acid （PDBA） on cis-diols, GHDA, which contains two pairs of hydroxyls in the cis form, can bind not only with AO/AuNPs or H/AuNPs, but also with PDBA to form boronate diester, thereby triggering the aggregation of AuNPs and causing the corresponding color change. As GalOx catalyzed specific substrates, the amount of Gal correlated with the production of GHDA and the extent of AuNPs aggregation, thus allowing a simple and easily operatable colorimetric method for Gal detection to be developed. Under the optimized experimental conditions, the ratios of absorbance at a wavelength of 617 nm to that at 536 nm vary linearly with the logarithmic values of Gal concentrations within a wide range of 500 nM to 5 mM. Moreover, this colorimetric method shows anti-interference capability and high sensitivity with a detection limit of 21 nM. Thus, a universal platform for accurate and specific colorimetric analysis can be established through the integration of chemoselective ligation with enzyme specific catalysis.

关键词： oxime/hydrazone ligation enzyme catalysis aminooxy/hydrazine-functionalized gold nanoparticles

Decentralized Event-Triggered Average Consensus for Multi-Agent Systems in CPSs with Communication Constraints

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IEEE/CAA Journal of Automatica Sinica 2015年第3期2卷 248-257页

作者： zhaoxia wang Minrui Fei Dajun Du Min Zheng the Shanghai Key Laboratory of Power Station Automation Technology School of Mechatronic Engineering and AutomationShanghai University the School of Electrical Engineering and Automation Qilu University of Technology the Shanghai Key Laboratoy of Power Station Automation Technology School of Mechatronic Engineering and AutomationShanghai University

The paper investigates decentralized event-triggered average consensus problem for multi-agent systems in cyberphysical systems (CPSs) with communication constraints. To reduce communication burden and improve the communication efficiency of multi-agent systems in CPSs, event-trigger is distributed at subsystem/agent level. A multi-agent system is then modeled as a reduced dimension hybrid system by taking into account decentralized event-triggered mechanism, communication delays and data dropouts within one framework. Some sufficient conditions for average consensus of each agent and an upper bound of communication delay and maximal allowable number of successive data dropouts (MANSD) are obtained, which can conveniently provide the relationship between the triggering parameters, communication constraints and the system stability. Specially, the quantitative relationship between the triggering parameters, MANSD and the system stability is derived. Finally, simulation results are given to illustrate the effectiveness of the proposed method. © 2014 Chinese Association of Automation.

关键词： Embedded systems Hybrid systems System stability

昼夜节律紊乱促进子宫内膜癌进展的作用机制研究

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中国生育健康杂志 2025年第1期36卷 51-55,69页

作者：赵乐王朝霞张三元山西医科大学第一临床医学院妇产科太原030001 山西医科大学第一医院妇科

目的探讨昼夜节律紊乱对子宫内膜癌节律基因CLOCK表达的影响及其参与疾病进展的可能机制。方法按照患者的年龄、孕产次、工作经历、教育信息及环境等背景匹配筛选出有夜班史的子宫内膜样腺癌6例为节律异常组,无夜班史6例为节律正常组,采... 详细信息

目的探讨昼夜节律紊乱对子宫内膜癌节律基因CLOCK表达的影响及其参与疾病进展的可能机制。方法按照患者的年龄、孕产次、工作经历、教育信息及环境等背景匹配筛选出有夜班史的子宫内膜样腺癌6例为节律异常组,无夜班史6例为节律正常组,采用qPCR法、Western blot检测两组节律基因CLOCK mRNA及蛋白质的表达;应用免疫组化法检测32例子宫内膜癌组织中上皮-间质转化相关表型蛋白(E-cadherin、vimentin)及CLOCK蛋白的表达,并分析其与临床病理特征的关系,以及CLOCK表达与上皮间质转化表型蛋白的相关性。结果qPCR及Western blot结果显示CLOCK mRNA及蛋白在节律异常组子宫内膜癌中的相对表达水平明显低于对照组(P<0.05);免疫组化提示CLOCK阴性率和E-cadherin阴性率、vimentin阳性率在有夜班史、FIGO分期II-III期、深肌层浸润的肿瘤中更高,差异有统计学意义(P<0.05)。CLOCK表达和E-cadherin、vimentin的相关性具有统计学意义(P<0.05)。结论夜班造成的昼夜节律紊乱下调了子宫内膜癌组织中CLOCK基因的表达,节律基因CLOCK参与肿瘤组织上皮-间质转化过程是子宫内膜癌进展的可能机制,可能是影响预后的重要因素。

关键词：子宫内膜癌生物钟基因节律基因CLOCK 上皮-间质转化

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CRACKING BEHAVIOR AND MECHANICAL ANALYSIS ON DUPLEX STRUCTURE UNDER CYCLIC LOADING

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Acta Metallurgica Sinica(English Letters) 1992年第2期5卷 107-111页

作者： wang zhaoxia CHAI Donglang LIU Jinghua Xi′an Jiaotong University,Xi′an,China Xi'an Jiaotong Univ Xi'an China

On the basis of stress-strain analysis under cyclic loading,the mechanism,origin and cyclic number of microcraek nucleation of duplex structure materials,saying ferrite plus martensite, have been experimentally studied and *** results are in fair mutual agreement. The morphology,distribution,size and relative quantities of the constitutional phase,especial ly strengthening phase,as well as the stress-strain distribution and variation in micro-region are directly influential in the origin of microcracks in duplex structure materials.

关键词： stress-strain analysis crack fatigue

Influence of surfactants used in surfactant-polymer flooding on the stability of Gudong crude oil emulsion

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Petroleum Science 2010年第2期7卷 263-267页

作者： Dong zhaoxia Lin Meiqin wang Hao Li Mingyuan Enhanced Oil Recovery Research Center China University of Petroleum Beijing 102249 China

The influences of an anionic-nonionic composite surfactant and petroleum sulfonate, used in surfactant-polymer flooding in Shengli Gudong oilfield, East China, on the interfacial properties of Gudong crude model oil and synthetic formation water was studied by measuring interfacial tension, interfacial viscoelasticity and Zeta potential. The in？ uence of the surfactants on the stability of Gudong water-in-oil （W/O） and oil-in-water （O/W） emulsions was evaluated by separating water from the W/O emulsion and residual oil in the aqueous phase of the O/W emulsion respectively. The results showed that the two kinds of surfactants, namely anionic-nonionic composite surfactant and petroleum sulfonate, are both able to decrease the interfacial tension between the oil phase and the aqueous phase and increase the surface potential of the oil droplets dispersed in the O/W emulsion, which can enhance the stability of the W/O and O/W crude oil emulsions. Compared with petroleum sulfonate, the anionic-nonionic composite surfactant is more interfacially active and able to enhance the strength of the interfacial film between oil and water, hence enhance the stability of the W/O and O/W emulsions more effectively.

关键词： Surfactant Gudong crude oil interfacial properties emulsion stability

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天然橡胶乳液的改性及应用研究进展

化工新型材料 2025年第2期53卷 48-52页

作者：马鹤龙黄煜琪王召霞赵蓉李冬娜马晓军天津科技大学轻工科学与工程学院天津300457 浙江大胜达包装股份有限公司杭州311215 山西工程技术学院阳泉045000

天然橡胶乳液(NRL)资源丰富可再生、价廉、环境友好、综合性能良好,在电绝缘材料、轮胎制造、医疗器械、航空和食品包装等领域具有广阔的应用前景。NRL改性分为化学改性和物理改性,介绍了化学改性(卤化、环氧化、接枝共聚)和物理改性(... 详细信息

天然橡胶乳液(NRL)资源丰富可再生、价廉、环境友好、综合性能良好,在电绝缘材料、轮胎制造、医疗器械、航空和食品包装等领域具有广阔的应用前景。NRL改性分为化学改性和物理改性,介绍了化学改性(卤化、环氧化、接枝共聚)和物理改性(添加天然高分子材料、纳米粒子)对NRL力学性能、粘接强度、热稳定性等的影响;综述了NRL在薄膜制品、胶粘剂、传感器及其他领域的应用进展,指出了NRL应用中存在的问题及改性发展方向。随着改性工艺的不断优化,NRL的研究将朝着低成本、高性能化方向发展。

关键词：天然橡胶乳液化学改性物理改性应用进展

Enterprises' Responsibility for Environment Protection in the Sharing Economy Era

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Meteorological and Environmental Research 2018年第4期9卷 74-79页

作者： wang zhaoxia Dai Ying Law School Zhejiang University of Technology Hangzhou 310023 China

The representative private enterprises in a town were studied, and how can they get out of the pollution predicament based on cooperation and participation in the background of sharing economy was analyzed, which has ... 详细信息

关键词： Sharing economy Social responsibility Private economy Cooperative pollution control

Transforming the Evaluation of the Rule of Law in China

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Social Sciences in China 2017年第1期38卷 85-105页

作者： Qian Hongdao wang zhaoxia Guanghua Law School Zhejiang University Law School Zhejiang University of Technology

Over the past ten years, China has seen a number of practices exploring the evaluation of the rule of law. It is important that we sum up the practical experience of these evaluations, analyze the problems they encounter, determine their direction nationwide, and put forward constructive ideas. With the issuing of the Decision of the Central Committee of the CPC on Some Major Issues Concerning Comprehensively Deepening the Reform and the Decision of the Central Committee of the CPC on Promoting a Number of Major Issues of the Rule of Law, evaluation of the rule of law faces a transformation both in theory and in practice. The key points in this transformation are having the right orientation, innovative mechanisms and solutions to tricky issues of quantitative evaluation; summing up evaluation of experience; and elevating evaluation to the level of theory. A precondition for its effective evaluation is a scientific mechanism for evaluating the rule of law; in turn, the key to a scientific evaluation is solving the problems in quantitative evaluation of the rule of law; and the necessary requirements for evaluation practice are summing up evaluation experience and elevating it to the level of theory. The key to innovative mechanisms for evaluating the rule of law lies in implementation of the third party evaluation model and in quantitative evaluation that starts with particular programs.

关键词： rule of law evaluation transformation of rule of law evaluation system ofindicators assessment criteria national governance

CRISPR/Cas9-mediated targeted gene correction in amyotrophic lateral sclerosis patient iPSCs

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Protein & Cell 2017年第5期8卷 365-378页

作者： Lixia wang Fei Yi Lina Fu Jiping Yang Si wang zhaoxia wang Keiichiro Suzuki Liang Sun Xiuling Xu Yang Yu Jie Qiao Juan Carlos Izpisua Belmonte Ze Yang Yun Yuan Jing Qu Guang-Hui Liu National Laboratory of Biomacromolecules CAS Center for Excellence in Biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China State Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China Department of Molecular and Cellular Physiology Stanford University School of Medicine Stanford CA 94305 USA Department of Neurology Peking University First Hospital Beijing 100034 China Gene Expression Laboratory Salk Institute for Biological Studies 10010 North Torrey Pines Road La Jolla CA 92037 USA Universidad Catolica San Antonio de Murcia (UCAM) Campus de los Jeronimos N 135 Guadalupe 30107 Murcia Spain Department of Gynecology and Obstetrics Peking University Third Hospital Beijing 100191 China Beijing Hospital of the Ministry of Health Beijing 100730 China Key Laboratory of Regenerative Medicine of Ministry of Education Institute of Aging and Regenerative Medicine Jinan University Guangzhou 510632 China Beijing Institute for Brain Disorders Capital Medical University Beijing 100069 China

Amyotrophic lateral sclerosis （ALS） is a complex neu- rodegenerative disease with cellular and molecular mechanisms yet to be fully described. Mutations in a number of genes including SOD1 and FUS are associated with familial ALS. Here we report the generation of induced pluripotent stem cells （iPSCs） from fibroblasts of familial ALS patients bearing SOD1＋1A27~c and FUS＋/GISe6A mutations, respectively. We further gener- ated gene corrected ALS iPSCs using CRISPR/Cas9 system. Genome-wide RNA sequencing （RNA-seq） analysis of motor neurons derived from SOD1＋~A272c and corrected iPSCs revealed 899 aberrant transcripts. Our work may shed light on discovery of early biomarkers and pathways dysregulated in ALS, as well as provide a basis for novel therapeutic strategies to treat ALS.

关键词： ALS CRISPR/Cas9 gene correction,iPSC disease modeling