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Comparative Analysis of the Exon-Intron Structure in Eukaryotic Genomes

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Yangtze Medicine 2017年第1期1卷 50-64页

作者： Yongfa Li Yanhua Xu zhaowu ma The second School of Clinical Medicine Yangtze University Jingzhou China Laboratory of Oncology Center for Molecular Medicine School of Medicine Yangtze University Jingzhou China Department of Oncology Central Hospital of Jingzhou Jingzhou China

The exon numbers and lengths vary in different eukaryotic species. With increasing completed genomic sequences, it is indispensable to reanalyze the gene organization in diverse eukaryotic genomes. We performed a large-scale comparative analysis of the exon-intron structure in 72 eukaryotic organisms, including plants, fungi and animals. We confirmed that the exon-intron structure varies massively among eukaryotic genomes and revealed some lineage-specific features of eukaryotic genes. These include a teleost-specific exon-intron structure pattern, relatively small introns and large exons in fungi and algae, and a gradual expansion of introns in vertebrates. Furthermore, the conservation analysis of exon-intron boundaries indicates that several bases near splice site junctions are different in introns with variable length among different species. After comparison, we identified a trend showing increases in intron densities and lengths in diverse species from fungi, plants, invertebrates to vertebrates, while it was the opposite in relation to exon lengths. The statistical properties of eukaryotic genomic organization suggest that genome-specific features are preserved by diverse evolutionary processes, which paves way for further research on the diversification of eukaryotic evolution.

关键词： Exon-Intron Structure Eukaryotic Genome Evolution

The Emerging Roles of Non-Coding RNAs in Cataract

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Yangtze Medicine 2020年第3期4卷 218-228页

作者： Jie Li Lei Ge Xiaoqin Wang zhaowu ma Ophthalmology Yangtze University Health Science Center Jingzhou China Department of Ophthalmology Jingzhou Central Hospital Jingzhou China School of Basic Medicine Health Science Center Yangtze University Jingzhou China

Non-coding RNAs (ncRNAs) are a large class of transcripts lacking evident protein coding potential, and play versatile roles in a diverse range of physiological and pathological processes. Mounting evidences have indicated that ncRNAs are aberrantly expressed in a wealth of diseases such as cataract. Cataract is a cloudy lens caused by radiation, age, drugs and other factors. NcRNAs, including microRNAs, long non-coding RNAs, circular RNAs, have been identified to regulate the occurrence and development of cataract. Current studies indicate that ncRNAs exert the multifaceted functions in the lens of cataract patients and have been proved as potential diagnostic biomarkers or therapeutic targets for cataracts. This review summarizes the study of relationship between the lens and ncRNAs, which can provide a novel insight into the pathogenesis of cataract.

关键词： Cataract PCO microRNAs Long Non-Coding RNAs Circular RNAs

The Construction and Implementation of a Cancer Precision Nursing Model

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Yangtze Medicine 2019年第1期3卷 1-9页

作者： Xunya Xiong Jun’e Hu zhaowu ma Xiaochun Peng Department of Medicine Yangtze University Jingzhou China Oncology Department Jingzhou Central Hospital Jingzhou China

This article reviews the precision nursing of cancer patients from three aspects: concept, system construction and clinical application. It is suggested that nurses should correctly understand the connotation of precision nursing, expand the research content, enrich the research methods, actively carry out the targeted cancer precision nursing practice, improve the quality of life of the patients, and then form the shape of the patient. It is a localized nursing care system that can provide the best medical care for every cancer patient.

关键词： Cancer Precision Nursing Nursing Practice Review

Oncolytic Herpes Simplex Virus ICP47 Deletion Reverses Tumor Immune Evasion

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Yangtze Medicine 2018年第4期2卷 214-224页

作者： Junting Cheng Yingying Wang Ying Zhang Wenqi Cai Yang Zhou Ziwen Han Xiaoqin Liu Xianwang Wang Xiaochun Pen Ying Xiang zhaowu ma Hongwu Xin The Second School of Clinical Medicine Health Science Center Yangtze University Jingzhou China Laboratory of Oncology Center for Molecular Medicine School of Basic Medicine Health Science Center Yangtze University Jingzhou China Department of Biochemistry and Molecular Biology School of Basic Medicine Health Science Center Yangtze University Jingzhou China Department of Laboratory Medicine School of Basic Medicine Health Science Center Yangtze University Jingzhou China Department of Pathophysiology School of Basic Medicine Health Science Center Yangtze University Jingzhou China

Herpes simplex virus (HSV) is an enveloped, double-stranded DNA virus that has been used with modification as oncolytic viruses (OVs) against a number of tumor types. OVs represent a new class of therapeutic agents that promote anti-tumour responses through a dual mechanism of action that is dependent on selective tumor cell killing and the induction of systemic anti-tumour immunity. Among OVs, HSVs preferentially replicate in and lyse cancer cells, leading to in situ autovaccination, adaptive anti-virus and anti-tumor immunity. Suppression of antitumor immunity after OV therapy has been observed and the molecular and cellular mechanisms of action are recently reported. ICP47, a small protein produced by the herpes simplex virus, is considered as an important factor in the evasion of cellular immune responses in HSV-infected cells. Therefore, reviewing the research status of ICP47 is certainly helpful to improve the anti-tumor effect of oncolytic HSVs (oHSVs). Here, this review will focus on the following contents: 1) Anti-tumor mechanism of OVs;2) Functions of early HSV genes;3) The mechanism of immune escape of ICP47;4) Recombinant HSV against cancer;5) The functional verification of ICP47 deletion. This review highlights the current understanding of recombinant HSVs against cancers.

关键词： Oncolytic Virus HSV ICP47 Anti-Tumor Immunity

The Role, Mechanism and Transcriptional Regulation of LAT in Herpes Simplex Virus Latency and Reactivation

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Yangtze Medicine 2020年第1期4卷 39-53页

作者： Ying Zhang Yingying Wang Junting Cheng Wenqi Cai Ziwen Han Yang Zhou Qi Huang Moyu Wang Xiaochun Peng Xianwang Wang zhaowu ma Ying Xiang Hongwu Xin Laboratory of Oncology Center for Molecular Medicine School of Basic Medicine Health Science Center Yangtze University Jingzhou China Department of Biochemistry and Molecular Biology School of Basic Medicine Health Science Center Yangtze University Jingzhou China Department of Pathophysiology School of Basic Medicine Health Science Center Yangtze University Jingzhou China Department of Laboratory Medicine School of Basic Medicine Health Science Center Yangtze University Jingzhou China

Herpes simplex virus (HSV) infection in the human body can be latent in neurons for long time and be reactivated leading to recurrence at high rate. Currently there is no effective clinical strategy for the prevention and treatment of the disease relapse. HSV LAT gene is expressed in large quantities and lytic genes are turned off leading to HSV latency. Disruption of the gene expression is thought to cause HSV reactivation and disease relapse. To reveal the essence of HSV latency and reactivation, we summarized and innovatively classified the role, mechanism and transcriptional regulation of LAT in HSV latency and reactivation. This review may have important implications for future studies on HSV latency and reactivation, HSV disease prevention and treatment, and safer and more effective oncolytic HSVs (oHSVs).

关键词： Herpes Simplex Virus (HSV) Oncolytic Herpes Simplex Virus (oHSV) Latency-Associated Transcript (LAT) Reactivation Immediate-Early Gene (IE Gene)

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基于气候年型的河南省冬小麦产量预测

中国农业科技导报 2022年第2期24卷 136-144页

作者：许鑫马兆务熊淑萍马新明程涛李海洋赵锦鹏河南农业大学信息与管理科学学院郑州450002 河南农业大学农学院郑州450002

气候变化是影响小麦产量的重要因素,因此科学划分气候年型,对于准确预测小麦产量至关重要。利用河南省15个气象站点(市、县)1984—2018年的温度数据划分暖温年、平温年、冷温年,依据降雨数据划分湿年、平水年、干年,组合成9种气候年型... 详细信息

气候变化是影响小麦产量的重要因素,因此科学划分气候年型,对于准确预测小麦产量至关重要。利用河南省15个气象站点(市、县)1984—2018年的温度数据划分暖温年、平温年、冷温年,依据降雨数据划分湿年、平水年、干年,组合成9种气候年型并分析其规律;结合小麦产量数据划分出丰产年、平产年、低产年,分析气候年型和产量年型的关系。然后结合地形土壤、气象等因素把河南划分为豫北麦区、豫中东部麦区、豫西麦区、豫南麦区和南阳盆地5个麦区。在此基础上,利用HP滤波法分离出气象产量和趋势产量,以气象因子驱动并利用BP神经网络构建模型预测气象产量,利用一元线性回归模型建模得到趋势产量,把两者产量叠加得出实际产量,从而实现小麦产量的预测。结果表明:河南省积温年型以暖温年、正常年型为主,降雨年型分布比较均匀,气候年型中以正常年和干年为主,暖湿年小麦高产频率最高,为76.9%,冷湿年小麦低产频率最高,为67.9%;积温是影响小麦产量波动的主要因素,暖年年型下小麦更容易丰产,冷年时小麦低产概率较高;利用气象产量和趋势产量分别建模叠加得出的小麦产量和实际产量相比,豫北、豫中东、豫西、豫南和南阳盆地五大麦区各模型的平均相对误差分别为0.31%、0.36%、0.58%、0.48%、0.38%,说明利用HP滤波和BP神经网络技术预测小麦产量是可行的。

关键词：小麦气象气候年型产量 HP滤波 BP神经网络

维普期刊数据库博看期刊

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HBV C区基因扩增与PCR条件的优化

中南民族大学学报（自然科学版） 2007年第1期26卷 30-32页

作者：马兆武袁媛陈强陈思礼中南民族大学生命科学学院武汉430074 华中师范大学生命科学学院武汉430079

目的:为提高PCR检测乙型肝炎病毒(HBV)的特异性和灵敏度,降低成本,对PCR条件进行优化.方法:将HBV特异性基因片段转化到大肠杆菌DH5α中,提取质粒,利用PCR扩增HBV C区基因,对PCR条件中的退火温度、Mg2+浓度进行优化,并比较3种不同Taq DN... 详细信息

目的:为提高PCR检测乙型肝炎病毒(HBV)的特异性和灵敏度,降低成本,对PCR条件进行优化.方法:将HBV特异性基因片段转化到大肠杆菌DH5α中,提取质粒,利用PCR扩增HBV C区基因,对PCR条件中的退火温度、Mg2+浓度进行优化,并比较3种不同Taq DNA聚合酶的灵敏度.结果:HBV C区基因扩增的最佳退火温度为58℃,最佳Mg2+浓度为1.5mmol/L,Biostar Taq DNA聚合酶扩增到10-6.讨论:对HBV C区基因进行了转化和PCR实验条件的优化,为扩大PCR在乙型肝炎病毒(HBV)检测领域中的应用提供实验依据.

关键词：乙型肝炎病毒聚合酶链反应条件优化

信息农机农艺技术融合的小麦智慧生产模式研究

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智慧农业 2019年第4期1卷 62-71页

作者：马新明马兆务许鑫席磊熊淑萍李海洋河南农业大学信息与管理科学学院河南郑州450046 河南农业大学农学院河南郑州450046

为研究智慧农业发展模式与实现途径,本研究设计了小麦产前、产中和产后各生产阶段信息技术与农机农艺融合的基本框架,即产前利用精准导航和激光平地技术实现对土地精准规范化作业,利用空间插值技术和变量施肥技术实现精准化播种与施肥;... 详细信息

为研究智慧农业发展模式与实现途径,本研究设计了小麦产前、产中和产后各生产阶段信息技术与农机农艺融合的基本框架,即产前利用精准导航和激光平地技术实现对土地精准规范化作业,利用空间插值技术和变量施肥技术实现精准化播种与施肥;产中利用物联网和图像处理技术开展营养诊断服务;产后运用传感器技术开展产量实时预测服务。完成并实现了普通农机装备的智能化改造和与农业生产相适应的播种收获装备的研发;研究了具有高效利用光热资源、提高产量和绿色发展的小麦生产优化种植模式;研发了与小麦产前品种播期播量选择和施肥推荐、产中苗情营养诊断、产后产量实时测报等相关系统,并在河南省进行了试验。试验结果表明,采用信息技术与农机农艺融合方案可使小麦增产18.4%,增加产投比16.7%和8.1%,表明信息技术与农机农艺融合的小麦智慧化生产模式是有效的、可行的。

关键词：信息农机农艺融合小麦智慧生产发展模式农业信息化

Interface Characteristics and Evolution Mechanism of W/CuCrZr in Hot Melt Explosion Welding

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稀有金属材料与工程 2024年第11期53卷 3077-3083页

作者： Sun Yuling Liang Hanliang Zhu Jiansheng ma Honghao Wang Luqing ZhangBingyuan Luo Ning Shen zhaowu CAS Key Laboratory of Mechanical Behavior and Design of materials University of Science and Technology of ChinaHefei 230026China PLA Army Academy of Artillery and Air Defense Hefei 230031China University of Science and Technology of China Hefei 230026China School of Mechanics and Civil Engineering China University of Mining and TechnologyXuzhou 221116China

Explosion welding was carried out on the basis of vacuum hot melt W/CuCrZr composite *** microscope,scanning electron microscope and energy dispersive X-ray spectroscope were used to observe the microscopic morphology of the bonding *** the same time,combined with finite element calculations,the evolution mechanism of the interface of the hot melt explosion welded W/CuCrZr composite plate was *** results show that the interface bonding of the hot melt explosion welded W/CuCrZr composite plate is good and there is a cross-melting zone with 3–8μm in thickness,but cracks are developed on the W *** numerical simulation reproduces the changes of pressure,stress,strain and internal energy at the bonding interface in the process of hot melt explosion *** location of the crack generated in the experiment coincides with the high stress position calculated by numerical *** high pressure and high temperature near the hot melt explosion welding interface further promote the bonding of the interface.

关键词： hot melt explosion welding W/CuCrZr interface characteristics numerical simulation evolution mechanisms