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Cell Research 2007年第6期17卷 512-519页

作者： Lin Xu Li YangHai Huang National Laboratory of plant Molecular Genetics, shanghai institute of plant physiology and ecology, shanghai institutes for biological sciences, chinese academy of sciences, 300 fenglin road, shanghai 2.0032, china National Laboratory of plant Molecular Genetics Shanghai Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Present address: IBMP CNRS Universite Louis Pasteur 67084 Strasbourg France Present address: Department of Biology University of Pennsylvania Philadelphia PA 19104 USA

Leaf morphogenesis requires the establishment of adaxial-abaxial polarity after primordium initiation from the shootapical meristem (SAM). Several families of transcription factors are known to play critical roles in promoting adaxial orabaxial leaf fate. Recently, post-transcriptional gene silencing pathways have been shown to regulate the establishmentof leaf polarity, providing novel and exciting insights into leaf development. For example, microRNAs (miR165/166)and a trans-acting siRNA (TAS3-derived tasiR-ARF) have been shown to repress the expression of several key transcrip-tion factor genes. In addition, yet another level of regulation, post-translational regulation, has been revealed recently bystudies on the role of the 26S proteasome in leaf polarity. Although our understanding regarding the molecular mecha-nisms underlying establishment of adaxial-abaxial polarity has greatly improved, there is still much that remains *** review aims to discuss recent progress, as well as the remaining questions, regarding the molecular mechanismsunderlying leaf polarity formation.

关键词： Arabidopsis leaf development polarity formation

Positive Charges on Lysine Residues of the Extrinsic 18 kDa Protein Are Important to Its Electrostatic Interaction with Spinach Photosystem Ⅱ Membranes

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Acta Biochimica et Biophysica Sinica 2005年第11期37卷 737-742页

作者： Jin-peng GAO Zhen-Hua YONG Feng zhang Kang-Cheng RUAN Chun-He XU Gen-Yun CHEN institute of plant physiology & ecology.shanghai institutes for biological sciences,chinese academy of sciences,shanghai 2.0032.china Laboratory of Proteomics,institute of Biochemistry and Cell Biology,shanghai institutes for biological sciences,chinese academy of sciences,shanghai 200031,china College of sciences,Zhejiang University.Hangzhou 310029,china Graduate School of the chinese academy of sciences,shanghai 2.0032.china institute of plant physiology & ecology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200032 China Graduate School of the chinese academy of sciencesshanghai 2.0032 China College of sciences Zhejiang UniversityHangzhou 310029 China Laboratory of Proteomics Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China

To determine the contribution of charged amino acids to binding with the photosystem Ⅱcomplex(PSⅡ),the amino or carboxyl groups of the extrinsic 18 kDa protein were modified with N-succinimidyl propionate(NSP)or glycine methyl ester(GME)in the presence of a water-soluble carbodiimide,*** on isoelectric point shift,4-10 and 10-14 amino groups were modified in the presence of2 and 4 mM NSP,***,3-4 carboxyl groups were modified by reaction with *** of negatively charged carboxyl groups with GME did not alter the binding activity of theextrinsic 18 kDa ***,the NSP-modified 18 kDa protein,in which the positively charged aminogroups had been modified to uncharged methyl esters,failed to bind with the PSⅡ membrane in the presenceof the extrinsic 23 kDa *** defect can not be attributed to structural or conformational alterationsimposed by chemical modification,as the fluorescence and circular dichroism spectra among native,GME-and NSP-modified extrinsic 18 kDa proteins were ***,we have concluded that the positive chargesof lysyl residues in the extrinsic 18 kDa protein are important for its interaction with PSⅡ membranes in thepresence of the extrinsic 23 kDa ***,it was found that the negative charges of carboxylgroups of this protein did not participate in binding with the extrinsic 23 kDa protein associated with PSⅡmembranes.

关键词： extrinsic 18 kDa protein circular dichroism electrostatic interaction chemical modification

HAL1 mediate salt adaptation in Arabidopsis thaliana

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Cell Research 2001年第2期11卷 142-148页

作者： YANG SU XIN, YAN XIU ZHAO, QUAN zhang, YU KE HE, HUI zhang, DA LUO institute of plant physiology, shanghai institutes for biological sciences, chinese academy of Science, 300 fenglin road, shanghai 2.0032, china Key Laboratory of plant Stress Research, Sh institute of plant physiology Shanghai Institutes for Biological Sciences Chinese Academyof Science Shanghai China Shanxi Normal University Xian China Key Laboratory of plant Stress Research Shandong Normal University Jinan China

The yeast HAL1 gene was introduced into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated transformation with vacuum infiltration under the control of CaMV 35S promoter. Thirty-three individual kanamycin resistant plants were obtained from 75,000 seeds. Southern blotting analysis indicated that HAL1 gene had been integrated into all of the transgenic plants’ genomes. The copy number of HAL1 gene in transgenic plants was mostly 1 to 3 by Southern analysis. Phenotypes of transgenic plants have no differences with wild type plants. several samples of transformants were self-pollinated, and progenies from transformed and non-transformed plants (controls) were evaluated for salt tolerance and gene expression. Measurement of concentrations of intracellular K+ and Na+ showed that transgenic lines were able to retain less Na+ than that of the control under salt stress. Results from different tests indicated the expression of HAL1 gene promotes a higher level of salt tolerance in vivo in the transgenic Arabidopsis plants.

关键词： Salt-tolerant Arabidopsis HAL1 gene engineering.

MORN motifs in plant PIPKs are involved in the regulation of subcellular localization and phospholipid binding

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Cell Research 2006年第5期16卷 466-478页

作者： Hui Ma~(1,*) Ying Lou~1 Wen Hui Lin~1 Hong wei Xue~1 ~1National Key Laboratory of plant Molecular Genetics,institute of plant physiology and ecology,shanghai institutes for biological Science(SIBS),chinese academy of sciences and Parmer Group of Max-Planck-institute of Molecular plant physiology(MPI-MP)on"plant Molecular physiology and Signal Transduction",300 fenglin road,2.0032 shanghai,china National Key Laboratory of plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) Chinese Academy of Sciences Partner Group of Max-Planck-institute of Molecular plant physiology (MPI-MP) on “plant Molecular physiology and Signal Transduction” Shanghai China Unit of Molecular Signal Transduction ERRB NICHD Bldg 49 Bethesda

Multiple repeats of membrane occupation and recognition nexus （MORN） motifs were detected in plant phosphati-dylinositl monophosphate kinase （PIPK）,a key enzyme in PI-signaling *** analysis indicates that allthe MORN motifs （with varied numbers at ranges of 7-9）,which shared high homologies to those of animal ones,werelocated at N-terminus and sequentially arranged,except those of OsPIPK1 and AtPIPK7,in which the last MORN motifwas separated others by an～100 amino-acid"island"region,revealing the presence of two kinds of MORN arrangementsin plant *** employing a yeast-based SMET （sequence of membrane-targeting） system,the MORN motifswere shown being able to target the fusion proteins to cell plasma membrane,which were further confirmed by expressionof fused MORN-GFP *** detailed analysis via deletion studies indicated the MORN motifs in OsPIPK1,together with the 104 amino-acid"island"region are involved in the regulation of differential subcellular localization,*** membrane or nucleus,of the fused *** Western blot analysis of the recombinant MORN polypep-tide,expressed in Escherichia coli,showed that MORN motifs could strongly bind to PA and relatively slightly to PI4Pand PI（4,5）*** results provide informative hints on mechanisms of subcellular localization,as well as regulationof substrate binding,of plant PIPKs.

关键词： membrane targeting PIPK MORN motif subcellular localization

DNA chip-based expression profile analysis indicates involvement of the phosphatidylinositol signaling pathway in multiple plant responses to hormone and abiotic treatments

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Cell Research 2004年第1期14卷 34-45页

作者： Wen Hui LIN, Rui YE, Hui MA, Zhi Hong XU, Hong wei XUE National Laboratory of plant Molecular Genetics, institute of plant physiology and ecology, shanghai institutes for biological sciences (SIBS), chinese academy of sciences and Partner Group of Max-Planck-institute of Molecular plant physiology (MPI-MP) on "plant Molecular physiology and Signal Transduction", 300 fenglin road, 2.0032 shanghai, china National Laboratory of plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences (SIBS) Chinese Academy of Sciences Partner Group of Max-Planck-institute of Molecular plant physiology (MPI-MP) on “plant Molecular physiology and Signal Transduction” Shanghai China

The phosphatidylinositol (PI) metabolic pathway is considered critical in plant responses to many environmental factors, and previous studies have indicated the involvement of multiple PI-related gene families during cellular responses. Through a detailed analysis of the Arabidopsis thaliana genome, 82 polypeptides were identified as being involved in PI signaling. These could be grouped into different families including PI synthases (PIS), PI-phosphate kinases (PIPK), phospholipases (PL), inositol polyphosphate phosphatases (IPPase), inositol polyphosphate kinases (IPK), PI transfer proteins and putative inositol polyphosphate receptors. The presence of more than 10 isoforms of PIPK, PLC, PLD and IPPase suggested that these genes might be differentially expressed during plant cellular responses or growth and development. Accordingly, DNA chip technology was employed to study the expression patterns of various isoforms. In total, 79 mRNA clones were amplified and used for DNA chip generation. Expression

关键词： phosphatidylinositol signal transduction DNA chip expression profile.

plant fertility defects induced by the enhanced expression of microRNA167

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Cell Research 2006年第5期16卷 457-465页

作者： peng Ru~1 Lin Xu~1 Hong Ma~(2,3) Hai Huang~(1,2) ~1National Laboratory of plant Molecular Genetics,shanghai institute of plant physiology and ecology,shanghai institute for biological sciences,300 fenglin road,shanghai 2.0032.china ~2SIBS-SJTU-PSU Joint Center for Life sciences,shanghai institute of plant physiology and Ecologgy,shanghai institute for biological sciences,300 fenglin road,shanghai 2.0032.china ~3Department of Biology and the Huck institute for the Life sciences,The Pennsylvania State University,University Park,PA 16802,USA National Laboratory of plant Molecular Genetics Shanghai Institute of Plant Physiology and Ecology Shanghai Institute for Biological Sciences Shanghai China SIBS-SJTU-PSU Joint Center for Life sciences Shanghai Institute of Plant Physiology and Ecology Shanghai Institute for Biological Sciences Shanghai China Department of Biology and the Huck institute for the Life sciences The Pennsylvania State University University Park USA

The plant hormone auxin plays a critical role in regulating plant growth and *** advances havebeen made in the understanding of auxin response pathways,primarily by the characterization of auxin response mu-tants in *** addition,microRNAs (miRNAs) have been shown to be critical regulators of genes importantfor normal plant development and ***,little is known about possible interactions between miRNAsand hormonal signaling during norlnal *** we show that an Arabidopsis microRNA,miR167,whichhas a complementary sequence to a portion of the A UXIN RESPONSE FACTOR6 (ARF6) and ARF8 mRNAs,can causetranscript degradation for ARFS,but not for *** report phenotypic characterizations of35S::MIR167b transgeniclines,and show that severe 35S::MIR167b transgenic lines had phenotypes similar to those of an arf6 arf8 double *** transgenic phenotypes suggest that miR167 may repress ARF6 at the level of *** demonstrate that thetransgenic plants are defective in all four whorls of floral *** the transgenic flowers,filaments were abnormallyshort,anthers could not properly release pollen,and pollen grains did not *** results provide an importantlink between the miRNA-mediated regulatory pathway of gene expression and the auxin signaling network promotingplant reproductive development.

关键词： Arabidopsis auxin response factor microRNA167 floral organ pollen germination

Isolation and functional characterization of the C-terminus of rice phos-phatidylinositol 4-kinase in vitro

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Cell Research 2003年第2期13卷 131-139页

作者： XIANG FENG KONG, ZHI HONG XU, HONG wei XUE, National Laboratory of plant Molecular Genetics, institute of plant physiology and ecology, shanghai institutes for biological Science (SIBS), The chinese academy of sciences Partner Group of Max-Planck-institute of Molecular plant physiology (MPI-MP) on "plant Molecular physiology and Signal Transduction", 300 fenglin road, 2.0032 shanghai, china National Laboratory of plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) The Chinese Academy of Sciences Partner Group el Max-Planck-institute of Molecular plant physiology (MPI-MP) on “plant Molecular physiology and Signal Transduction” Shanghai China

A partial rice (Oryza sativa L.) cDNA clone. OsPMK1c, was isolated through screening of a cDNA library constructed from tillering materials. OsPI4Klc encoded a peptide of 608 amino acids with a calculated molecular mass of 68.4 kDa. The OsPI4Klc peptide shared high homology and possessed the highly conserved domains present in most isolated cloned PI4-kinases, i.e. a lipid kinase unique (LKU) domain and a catalytic (CAT) domain. A region with similarity to pleckstrin homology (PH) domain was present in OsPI4K1c as well. Further comparison with genomic sequences in databases revealed that OsPI4K1c is located at the 3'-end of a putative rice PI 4-kinase coding gene OsPI4K1, and its coding region corresponded to the C-terminal half of OsPI4Kl protein. Twelve exons (49-562 bp in size) and 11 introns (77-974 bp in size) were identified in OsPI4K1c. The recombinant protein expressed in Escherichia coli phosphorylates phosphatidylinositol at the D4 position of the inositol ring. OsPI4K1 transcript levels were

关键词： Oryza sativa phosphatidylinositol 4-kinase.

Preliminary studies on differential defense responses induced during plant communication

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Cell Research 2005年第3期15卷 187-192页

作者： Jin Ying peng1,3,Zhong Hai LI2,Hui XIANG1,3,Jian Hua HUANG1,3,Shi Hai JIA1,3,Xue Xia MIAO1,Yong Ping HUANG1* 1institute of plant physiology and ecology,shanghai institute for biological sciences,Graduate School of the chinese academy of sciences,chinese academy of sciences,300 fenglin road,shanghai 2.0032.china 2chinese National Human Genome Center at shanghai,250 Bibo road,shanghai 201203,china 3Graduate School of the chinese academy of sciences,Beijing 100039,china institute of plant physiology and ecology Shanghai Institute for Biological Sciences Graduate School of the Chinese Academy of Sciences Chinese Academy of Sciences Shanghai China Graduate School of the chinese academy of sciences Beijing China chinese National Human Genome Center at shanghai Shanghai China

We compared the expression patterns of three representative genes in undamaged tomato and tobacco plants in response to exposure to either tomato or tobacco fed on by Helicoverpa armigera (cotton bollworm). When tomato and tobacco, two species of one family, were incubated in the chambers with the tomato plants damaged by the cotton bollworm, the expression of the PR1, BGL2, and PAL genes was up-regulated in leaves of both plants. However, the levels of gene expression were significantly higher in the tomato than that in the tobacco. In addition, the activities of enzymes, peroxidase, polyphenol oxidase, and lipoxygenase were found to be higher in the tomato than those in the tobacco. Similar results were obtained when the damaged plants were replaced by the tobacco.

关键词： plant-plant communication tomato (Lycopersicon esculentum) tobacco (Nicotiana tabacum) cotton boll- worm (Helicoverpa armigera) inducible defenses.

Identification of ABA-responsive genes in rice shoots via cDNA macroar- ray

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Cell Research 2003年第1期13卷 59-68页

作者： FANG LIN, SHOU LING XU, wei MIN NI, ZHAO QING CHU, ZHI HONG XU, HONG wei XUENational Laboratory of plant Molecular Genetics, institute of plant physiology and ecology, shanghai institutes for biological Science (SIBS), chinese academy of sciences and Partner Group of Max-Planck-institute of Molecular plant physiology (MPI-MP) on "plant Molecular physiology and Signal Transduc-tion", 300 fenglin road, 2.0032 shanghai, china National Laboratory of plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) Chinese Academy of Sciences Partner Group of Max-Planck-institute of Molecular plant physiology (MPI-MP) on plant Molecular physiology and Signal Transduction Shanghai China

Phytohormone abscisic acid (ABA) was critical for many plant growth and developmental processes including seed maturation, germination and response to environmental factors. With the purpose to detect the possible ABA related signal transduction pathways, we tried to isolate ABA-regulated genes through cDNA macroarray technology using ABA-treated rice seedling as materials (under treatment for 2, 4, 8 and 12 h). Of 6144 cDNA clones tested, 37 differential clones showing induction or suppression for at least one time, were isolated. Of them 30 and 7 were up- or down-regulated respectively. Sequence analyses revealed that the putative encoded proteins were involved in different possible processes, including transcription, metabolism and resistance, photosynthesis, signal transduction, and seed maturation. 6 cDNA clones were found to encode proteins with unknown functions. Regulation by ABA of 7 selected clones relating to signal transduction or metabolism was confirmed by reverse transcription PCR. In add

关键词： Oryza saliva abscisic acid (ABA) cDNA macroarray responsive genes signal transduc- tion

Different expression patterns of duplicated PHANTASTICA-like genes in Lotus japonicus suggest their divergent functions during compound leaf development

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Cell Research 2005年第8期15卷 665-677页

作者： Jiang Hong LUO1, Jun YAN1, Lin WENG1, Jun YANG1, Zhong ZHAO3, Jiang Hua CHEN1, Xiao He HU1, Da LUO1,2,* 1National Laboratory of plant Molecular Genetics, institute of plant physiology and ecology, shanghai institutes for Bio- logical sciences, and Graduate School of the chinese academy of sciences, chinese academy of sciences, 300 fenglin road, shanghai 2.0032, china 2School of Life Science and Biotechnology, shanghai Jiao Tong University, 1954 Huashan road, shanghai 200030, china 3Department of Biochemistry, School of Life sciences, Fudan University, shanghai 200433, china National Laboratory of plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences and Graduate School of the Chinese Academy of Sciences Chinese Academy of Sciences Shanghai China Department of Biochemistry School of Life Sciences Fudan University Shanghai China School of Life Science and Biotechnology Shanghai Jiao Tong University Shanghai China

Recent studies on leaf development demonstrate that the mechanism on the adaxial-abaxial polarity pattern formation could be well conserved among the far-related species, in which PHANTASTICA (PAHN)-like genes play important roles. In this study, we explored the conservation and diversity on functions of PHAN-like genes during the compound leaf development in Lotus japonicus, a papilionoid legume. Two PHAN-like genes in L. japonicus, LjPHANa and LjPHANb, were found to originate from a gene duplication event and displayed different expression patterns during compound leaf development. Two mutants, reduced leaflets1 (rel1) and reduced leaflets3 (rel3), which exhibited decreased adaxial identity of leaflets and reduced leaflet initiation, were identified and investigated. The expression patterns of both LjPHANs in rel mutants were altered and correlated with abnormalities of compound leaves. Our data suggest that LjPHANa and LjPHANb play important but divergent roles in regulating adaxial-abaxial polarity of compound leaves in L. japonicus.

关键词： adaxial identity LjPHANa LjPHANb gene duplication rel mutants Lotus japonicus.