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Horticultural Plant Journal 2024年第3期10卷 641-656页

作者： Kai Liu zhaolin liang An Yang Jiadi Yan Peihua Cong Xiaolei Han Caixia Zhang Key Laboratory of Horticultural Crop Germplasm Utilization of the Ministry of Agriculture and Rural Affairs(MOA) Research Institute of PomologyChinese Academy of Agricultural SciencesXingchengLiaoning 125100China

Apple leaf spot,caused by the Alternaria alternata apple pathotype(AAAP),is an important fungal disease of *** understand the molecular basis of resistance and pathogenesis in apple leaf spot,the transcriptomes of two apple cultivars‘Hanfu'(HF)(resistant)and‘Golden Delicious'(GD)(susceptible)were analyzed at 0,6,18,24 and 48 h after AAAP inoculation by *** each time point,a large number of significantly differentially expressed genes(DEGs)were screened between AAAP-inoculated and uninoculated apple *** of the common DEGs at four time points revealed significant differences in the resistance of‘HF'and‘GD'apple to AAAP ***,RNL,and JA signal-related genes were upregulated in both cultivars to restrict AAAP ***,genes encoding CNLs,TNLs,WRKYs,and AP2s were only activated in‘HF'as part of the resistance response,of which,some play major roles in the regulation of ET and SA signal *** analysis showed that many DEGs with opposite expression trends in the two hosts may play important regulatory roles in response to AAAP *** expression of one such gene MdERF110 in‘GD'apple leaves improved AAAP ***,this study highlights the reasons for differential resistance to AAAP infection between‘HF'and‘GD'apples which can theoretically assist the molecular breeding of disease-resistant apple crops.

关键词： Apple Transcriptome Alternaria alternata apple pathotype Infection Defense response

MdAIL5 overexpression promotes apple adventitious shoot regeneration by regulating hormone signaling and activating the expression of shoot development-related genes

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Horticulture Research 2023年第11期10卷 45-58页

作者： Kai Liu An Yang Jiadi Yan zhaolin liang Gaopeng Yuan Peihua Cong Liyi Zhang Xiaolei Han Caixia Zhang Apple Breeding Chinese Academy of Agricultural Sciences Research Institute of PomologyXingcheng 125100China Key Laboratory of Horticultural Crops Germplasm Resources Utilization Ministry of Agriculture and Rural Affairs of the People’s Republic of ChinaXingcheng 125100China Shandong Academy of Grape Shandong Academy of Agricultural SciencesJinan 250100China Zhengzhou Fruit Research Institute Chinese Academy of Agricultural SciencesZhengzhou 450009China

Adventitious shoot(AS)regeneration is a significant factor in the genetic transformation of horticultural *** is also a noteworthy approach to their vegetative *** regeneration remains highly dependent on the genotype or maturity of *** here found that the AS regeneration abilities of apple leaves were positively correlated with MdAIL5 ***5 overexpression dramatically increased AS regeneration ***,MdAIL5 overexpression could restore the AS formation ability of explants to a certain extent,which was lost with an increase in *** hormone detection revealed that MdAIL5 overexpression changed the contents of auxin,cytokinin(CK),and other hormones in apple *** analysis revealed that many genes related to auxin,CK,and brassinolide signaling pathways were significantly and differentially expressed between MdAIL5-overexpressing transgenic apple and wild-type apple *** one-hybrid assays,the electrophoretic mobility shift assay,and the dual-luciferase reporter assay revealed that MdAIL5 directly binds to MdARF9 and MdHB14 promoters and positively affects their *** here established a model of MdAIL5 regulating AS formation,which acts as a theoretical basis for facilitating genotype-or explant maturity-independent AS regeneration in the future.

关键词： expression maturity regeneration

一种利用基因表达变化检测、鉴别抗生素的新方法

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生物工程学报 2010年第1期26卷 93-99页

作者：赵冰孙赵麟杨亮梁海华沈立新段康民西北大学生命科学学院西部资源生物与现代生物技术教育部重点实验室西安710069

本研究利用基因表达变化构建一个高通量检测筛选抗生素化合物的新方法。采用含luxCDABE报道子的质粒pCS26和pMS402为载体,构建大肠杆菌重组质粒、筛选铜绿假单胞菌以及沙门氏菌的随机启动子库,获得对抗生素有反应的启动子-报道子融合体... 详细信息

本研究利用基因表达变化构建一个高通量检测筛选抗生素化合物的新方法。采用含luxCDABE报道子的质粒pCS26和pMS402为载体,构建大肠杆菌重组质粒、筛选铜绿假单胞菌以及沙门氏菌的随机启动子库,获得对抗生素有反应的启动子-报道子融合体,构成抗生素筛选体系。通过重复实验筛选出15个启动子-报道子融合体,它们对不同抗生素有不同的反应,通过这一系统可以检测样品中的抗生素,并初步判断与现有抗生素的异同。使用这种方法可以进行高通量筛选潜在的抗菌物质。

关键词：抗生素筛选启动子报道子

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引进薄煤层开采新工艺探索节能减排新途径

山西焦煤科技 2009年第10期33卷 44-46页

作者：郝志强梁肇林西山煤电股份公司西曲矿

介绍为了安全高效地采用螺旋钻采煤机开采薄煤层应做到:巷道布置合理,熟练操作设备并在生产过程中不断改进设备性能,钻采过程中,加强地质预报工作,加强工作面运输顺槽的顶板管理和矿压观测,制定切实有效的采空区处理措施,加强施工技术管... 详细信息

介绍为了安全高效地采用螺旋钻采煤机开采薄煤层应做到:巷道布置合理,熟练操作设备并在生产过程中不断改进设备性能,钻采过程中,加强地质预报工作,加强工作面运输顺槽的顶板管理和矿压观测,制定切实有效的采空区处理措施,加强施工技术管理,不断提高施工队伍技术素质,以达到快速、高效开采薄煤层的目的,同时开辟一条节能减排新途径。

关键词：节能减排开采薄煤层螺旋钻采煤机

转座子介导MYB在果树花青素合成中的调控作用

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中国果树 2022年第2期 7-11页

作者：江春阳杜美娥梁兆林丛佩华张利义中国农业科学院果树研究所农业农村部园艺作物种质资源利用重点实验室辽宁兴城125100 鄂尔多斯生态环境职业学院

着色是果实的重要品质性状,也是果树育种计划的重要目标之一。随着更多高质量的果树基因组测序完成,与花青素相关的果实着色的分子调控机制已取得了重要进展,特别是转座子作为重要调控元件控制MYB转录因子影响果实着色的机制,在一些重... 详细信息

着色是果实的重要品质性状,也是果树育种计划的重要目标之一。随着更多高质量的果树基因组测序完成,与花青素相关的果实着色的分子调控机制已取得了重要进展,特别是转座子作为重要调控元件控制MYB转录因子影响果实着色的机制,在一些重要果树上已被揭示。综述了转座子调控果树果实着色的相关进展,特别是基于基因组给出了控制梨果实着色的2个重要转录因子MYB10和MYB114启动子区的转座子插入注释,希望能为红色梨以及其他园艺植物果实着色的分子生物学研究提供有价值的线索和思路。

关键词：果实着色转座子 MYB转录因子花青素

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深圳前海世茂金融中心塔楼结构设计及分析

建筑结构 2015年第24期45卷 58-65页

作者：马晖吕海双罗承师汪洋林奉军梁兆麟信瑛佩奥雅纳工程顾问深圳518048

深圳前海世茂金融中心塔楼结构高度为303.9m,首层至屋面每层绕中心相对下层旋转0.68°,共旋转45°。塔楼为框架-核心筒形式,外框为钢管混凝土分段双向斜直柱+钢梁,核心筒为钢筋混凝土结构。旋转外框架不便设置伸臂桁架,通过优化结构平... 详细信息

深圳前海世茂金融中心塔楼结构高度为303.9m,首层至屋面每层绕中心相对下层旋转0.68°,共旋转45°。塔楼为框架-核心筒形式,外框为钢管混凝土分段双向斜直柱+钢梁,核心筒为钢筋混凝土结构。旋转外框架不便设置伸臂桁架,通过优化结构平面形状和体型、利用周边地形地貌条件减小风荷载,解决了刚度和舒适度问题。外框在重力作用下形成水平力环流,对核心筒形成扭矩,通过对传力路径、节点构造、重力下整体水平位移及整体稳定性的分析并采取措施,达到设计目的。

关键词：深圳前海世茂金融中心旋转框架分段双向斜直柱风荷载水平力环流扭矩传力路径

An image segmentation method of pulverized coal for particle size analysis

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International Journal of Mining Science and Technology 2023年第9期33卷 1181-1192页

作者： Xin Li Shiyin Li liang Dong Shuxian Su Xiaojuan Hu zhaolin Lu Affiliated Hospital of China University of Mining and Technology Xuzhou 221002China School of Information and Control Engineering China University of Mining and TechnologyXuzhou 221116China School of Chemical Engineering&Technology China University of Mining and TechnologyXuzhou 221116China Advanced Analysis and Computation Center China University of Mining and TechnologyXuzhou 221116China

An important index to evaluate the process efficiency of coal preparation is the mineral liberation degree of pulverized coal,which is greatly influenced by the particle size and shape distribution acquired by image ***,the agglomeration effect of fine powders and the edge effect of granular images caused by scanning electron microscopy greatly affect the precision of particle image *** this study,we propose a novel image segmentation method derived from mask regional convolutional neural network based on deep learning for recognizing fine coal ***,an atrous convolution is introduced into our network to learn the image feature of multi-sized powders,which can reduce the missing segmentation of small-sized agglomerated ***,a new mask loss function combing focal loss and dice coefficient is used to overcome the false segmentation caused by the edge *** final comparative experimental results show that our method achieves the best results of 94.43%and 91.44%on AP50 and AP75 respectively among the comparison *** addition,in order to provide an effective method for particle size analysis of coal particles,we study the particle size distribution of coal powders based on the proposed image segmentation method and obtain a good curve relationship between cumulative mass fraction and particle size.

关键词： Pulverized coal Image segmentation Deep learning Particle size analysis

Technical measures to achieve a cleaner production mode for recycled paper mills

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Frontiers of Environmental Science & Engineering 2010年第4期4卷 466-474页

作者： Chao HE zhaolin GU Shucheng YANG Jidong liang Weina DAI Yanling HE School of Mechanical Engineering Xi’an Jiaotong UniversityXi’an 710049China School of Human Settlements and Civil Engineering Xi’an Jiaotong UniversityXi’an 710049China Department of Environmental Science and Engineering Xi’an Jiaotong UniversityXi’an 710028China

China’s paper production reached 79.8×10^(6) t in 2008 and ranked number one in the *** of its high consumption of water,energy and materials and its serious pollution,the present processes are not likely to be *** alternative,the closed Water Loop-Papermaking Integration(WLPI)method,is put forward in this *** WLPI method can be realized in a recycled paper mill by adding technologies and using recycled *** industrial case studies have shown that a large quantity of water,energy and materials can be saved,and the quantity of waste sludge and wastewater discharge was minimized by using the WLPI *** design of the water reuse system,control of calcium hardness,water recycling and minimal waste sludge are *** technology plays an important role in the WLPI method to lower cost,energy use and *** the brown paper and coated white board production,zeroeffluent discharge can be *** water consumption is only 1-2m^(3)·t^(-1).For the paper mills with deinking and bleaching processes,about 10 m^(3)·t^(-1) of fresh water and a similar amount of effluent discharge are *** saving using anaerobic technology is 70%when recycled water is used in comparison with the conventional activated sludge *** sludge can be decreased to about 5%of the initial process due to reuse of the waste sludge and the lower bio-sludge production of the anaerobic process.

关键词： water reuse zero discharge anaerobic treatment recycled paper cleaner production

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填料的表面润湿和传质研究

石油化工高等学校学报 1996年第1期9卷 6-10页

作者：梁朝林黄洪杨卓如陈焕钦广东石油化工商等专科学校广东茂名525000 华南理工大学广东广州510641

填料表面的润湿性能直接关系到填料塔中气液两相的传质效果，通过选择适当材质和溶剂，对填料的表面进行物理－化学改性、改变填料表面粗糙度等办法，提高液相对塔科料表面的润湿性能，改善传质效果；并且寻找出填料固－液润湿角与体积... 详细信息

填料表面的润湿性能直接关系到填料塔中气液两相的传质效果，通过选择适当材质和溶剂，对填料的表面进行物理－化学改性、改变填料表面粗糙度等办法，提高液相对塔科料表面的润湿性能，改善传质效果；并且寻找出填料固－液润湿角与体积传质系数的关系。

关键词：填料传质润湿角表面润湿

Nanoporous Metal-Organic Frameworks for On-board Methane Storage

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Nanoporous Metal-Organic Frameworks for On-board Methane Sto...