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Immunohistochemical prognostic markers of esophageal squamous cell carcinoma:a systematic review

Chinese Journal of Cancer 2017年第11期36卷 592-608页

作者： Chunni Wang Jingnan Wang zhaoli chen Yibo Gao Jie He Department of Thoracic Surgery National Cancer Center/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeScience Building Center for Cancer Precision Medicine Chinese Academy of Medical Sciences and Peking Union Medical College

Background: Esophageal squamous cell carcinoma(ESCC) is an aggressive malignancy, with a high incidence and poor prognosis. In the past several decades, hundreds of proteins have been reported to be associated with the prognosis of ESCC, but none has been widely accepted to guide clinical care. This study aimed to identify proteins with great potential for predicting prognosis of ***: We conducted a systematic review on immunohistochemical(IHC) prognostic markers of ESCC according to the 2009 Preferred Reporting Items for Systematic Reviews and Meta-Analyses(PRISMA) Guidelines. Literature related to IHC prognostic markers of ESCC were searched from PubMed, Embase, Web of Science, and Cochrane Library until January 30 th, 2017. The risk of bias of these original studies was evaluated using the Quality in Prognosis Studies(QUIPS) ***: We identified 11 emerging IHC markers with reproducible results, including eight markers [epidermal growth factor receptor(EGFR), Cyclin D1, vascular endothelial growth factor(VEGF), Survivin, Podoplanin, Fascin,phosphorylated mammalian target of rapamycin(p-mTOR), and pyruvate kinase M2(PKM2)] indicating unfavorable prognosis and 3 markers(P27, P16, and E-cadherin) indicating favorable prognosis of ***: Strong evidence supports that these 11 emerging IHC markers or their combinations may be useful in predicting prognosis and aiding personalized therapy decision-making for ESCC patients.

关键词： Esophageal squamous cell carcinoma Prognosis Survival Immunohistochemical markers

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Growth and Transcriptomics Analysis ofMichelia macclurei Dandy Plantlets with Different LED Quality Treatments

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Phyton-International Journal of Experimental Botany 2023年第10期92卷 2891-2906页

作者： zhaoli chen Ying Liu Bingshan Zeng Qingbin Jiang Shengkun Wang Xiangyang Li Research Institute of Tropical Forestry Chinese Academy of ForestryGuangzhou510520China

Michelia macclurei Dandy is a significant tree species that has extensive cultivation for forestry and horticulture purposes in southern China,owing to its economic and practical *** quality influences plantlet growth and development during tissue ***,the growth characteristic and molecular regulation of *** under different light quality conditions are not well understood *** this study,we investigated the morphological,chlorophyll content,and transcriptomic responses of *** plantlets under different light-emitting diode(LED)qualities,including white,blue,and red *** results showed that blue light significantly increased plant height(21.29%)and leaf number(18.65%),while red light decreased plant height and leaf number by 7.53%and 16.49%,*** addition,the plantlets’chlorophyll content and etiolation rate were significantly reduced by blue and red light quality compared to white *** to white light,blue light had a negative effect,leading to decreased rooting rate(64.28%),root number(72.72%),and root length(75.86%).Conversely,red light had a positive effect,resulting in increased rooting rate(24.99%),root number(109.58%),and root length(72.72%).Transcriptome analysis identified 54 differentially expressed genes(DEGs)in three groups that consisted of blue light *** light(BL-vs-WL),red light *** light(RL-vs-WL),and red light *** light(RL-vs-BL).Specifically,21,7,and 41 DEGs were identified in the three groups,*** DEGs found in the RL-vs-WL and BL-vs-WL groups were involved in plant hormone signaling,nitrogen metabolism,and phenylpropanoid biosynthesis pathways,which suggests that *** plantlets adapt to the changes of light quality via modulating gene ***,our study provides valuable insights for understanding the molecular and morphological responses of *** plantlets under different light qualities.

关键词： Michelia macclurei plantlets growth characteristics transcriptome

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Transcriptomic Analysis of Aflatoxin B1-Regulated Genes in Rat Hepatic Epithelial Cells

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Transactions of Tianjin University 2014年第6期20卷 451-457页

作者： Yang Liu Ji Jing Li Guanghui Li Junwen chen zhaoli Wang Haiyong School of Chemical Engineering and Technology Tianjin UniversityTianjin 300072China School of Environmental Science and Technology Tianjin UniversityTianjin 300072China Department of Chemistry Tangshan Normal UniversityTangshan 063000China Institute of Hygiene and Environmental Medicine Academy of Military Medical ScienceTianjin 300050China

Aflatoxins are the most popular hepatotoxicants. Chronic exposure to aflatoxins leads to a wide variety ofliver diseases, such as hepatocellular carcinoma. In this study, we analyzed the genome wide expression profiles ofaflatoxin B1-induced rat hepatic epithelial cells. The expression of 325, 184 and 199 special genes was altered whenexposed to 0.03, 0.1 and 0.2 μmol/L aflatoxin B1 respectively, and 239 genes were commonly expressed. After thefunctional analysis on these dose-special genes, we determined several key pathways related to hepatotoxicity, such asTGF-beta signaling pathway, tight junction, adherens junction, the regulation of actin cytoskeleton, ErbB signalingpathway, p53 signaling pathway, pathways in cancer and axon guidance. Common genes were mainly associated withfocal adhesion, ECM-receptor interaction, and cell adhesion molecules. Gene ontology annotations showed a goodconcordance with these pathways. The quantitative real-time polymerase chain reaction（PCR） analysis of selectedgenes showed similar patterns in microarrays. The toxicogenomic study provides a better understanding of molecularmechanisms of aflatoxins.

关键词： aflatoxin microarray gene ontology Kyoto encyclopedia of genes and genomes(KEGG) pathway hepatotoxicity

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The regulatory roles of DDIT4 in TDCIPP-induced autophagy and apoptosis in PC12 cells

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Journal of Environmental Sciences 2023年第3期125卷 823-830页

作者： Li Li Lingyi Xi JinWu Zunquan Zhao Youliang chen Weili Liu Zhihui Pan Mingzhu Liu Danfeng Yang zhaoli chen Yanjun Fang Tianjin Institute of Environmental and Operational Medicine Tianjin 300050China Luoyang Central Hospital Affiliated to Zhengzhou University Luoyang 471000China School of Food and Biological Engineering Shaanxi University of Science and TechnologyXi’an 710021China China Academy of Safety Science and Technology Beijing 100012China

Tris(1,3-dichloro-2-propyl) phosphate(TDCIPP) is a commonly used organophosphatebased flame retardant and can bio-accumulate in human tissues and organs. As its structure is similar to that of neurotoxic organophosphate pesticides, the neurotoxicity of TDCIPP has raised widespread concerns. TDCIPP can increase neuronal apoptosis and induce ***, its regulatory mechanism remains unclear. In this study, we found that the expression upregulation of the DNA Damage-Inducible Transcript 4(DDIT4) protein, which might play essential roles in TDCIPP-induced neuronal autophagy and apoptosis, was observed in TDCIPP-treated differentiated rat PC12 cells. Furthermore, we determined the protective effect of the DDIT4 suppression on the autophagy and apoptosis induced by TDCIPP using Western blot(WB) and Flow cytometry(FACS) analysis. We observed that TDCIPP treatment increased the DDIT4, the autophagy marker Beclin-1, and the microtubule-associated protein light chain 3-II(LC_(3)II) expressions and decreased the mTOR phosphorylation levels. Conversely, the suppression of DDIT4 expression increased the p-mTOR expression and decreased cell autophagy and apoptosis. Collectively, our results revealed the function of DDIT4 in cell death mechanisms triggered by TDCIPP through the m TOR signaling axis in differentiated PC12 cells. Thus, this study provided vital evidence necessary to explain the mechanism of TDCIPP-induced neurotoxicity in differentiated PC12 cells.

关键词： TDCIPP DDIT4 Autophagy Neurotoxicity

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LAMC2 regulates proliferation, migration, and invasion mediated by the Pl3K/AKT/mTOR pathway in oral

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Oncology Research 2023年第4期31卷 481-493页

作者： FAYU SHAN LANLAN LIANG CHONG FENG HONGBAO XU ZIROU WANG WEILI LIU LINGLING PU zhaoli chen GANG chen XINXING WANG Department of Environmental Medicine Tianjin Institute of Environmental and Operational MedicineTianjin300070China School and Hospital of Stomatology Tianjin Medical UniversityTianjin300050China

Background:Oral squamous cell carcinoma(OSCC)is a common malignant ***,Laminin Gamma 2(LAMC2)has been shown to be abnormally expressed in OSCC;however,how LAMC2 signaling contributes to the occurrence and development of OSCC and the role of autophagy in OSCC has not been fully *** study aimed to analyze the role and mechanism of LAMC2 signaling in OSCC and the involvement of autophagy in ***:To explore the mechanism by which LAMC2 is highly expressed in OSCC,we used small interfering RNA(siRNA)to knock down LAMC2 to further observe the changes in the signaling ***,we used cell proliferation assays,Transwell invasion assays,and wound-healing assays to observe the changes in OSCC proliferation,invasion,and ***-LC3 was used to detect the level of autophagy intensity.A cell line-derived xenograft(CDX)model was used to detect the effect of LAMC2 on tumor growth in ***:This study found that the level of autophagy was correlated with the biological behavior of *** downregulation of LAMC2 activated autophagy and inhibited OSCC proliferation,invasion,and metastasis via inhibiting the PI3K/AKT/mTOR ***,autophagy has a dual effect on OSCC,and the synergistic downregulation of LAMC2 and autophagy can inhibit OSCC metastasis,invasion,and proliferation via the PI3K/AKT/mTOR ***:LAMC2 interacts with autophagy to regulate OSCC metastasis,invasion,and proliferation via the PI3K/AKT/mTOR ***2 down-regulation can synergistically modulate autophagy to inhibit OSCC migration,invasion,and proliferation.

关键词： LAMC2 OSCC Autophagy PI3K/AKT/mTOR pathway 3-Methyladenine Rapamycin

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Over-expression of small ubiquitin-like modifier proteases 1 predicts chemo-sensitivity and poor survival in non-small cell lung cancer

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Chinese Medical Journal 2014年第23期127卷 4060-4065页

作者： Mu Juwei Zuo Yong Yang Wenjing chen zhaoli Liu Ziyuan Tu Jun Li Yan Yuan Zuyang cheng Jinke He Jie Department of Thoracic Surgical Oncology Cancer Institute & Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100021 China Department of Biochemistry and Molecular Cell Biology Shanghai Jiao Tong University School of Medicine Shanghai 200025 China

Background Non-small cell lung cancer （NSCLC） is one of the most common malignant *** the advances in therapy over the years,its mortality remains *** aim of this study was to evaluate the expression of small ubiquitin-like modifier （SUMO） proteases 1 （SENP1） in NSCLC tissues and its role in the regulation of vascular endothelial growth factor （VEGF） *** also investigated the association between the expression level of SENP1 and the clinicopathological features and survival of the *** A SENP1 small interfering RNA （siRNA） was constructed and transfected into the NSCLC *** gene expression was analyzed by real-time polymerase chain reaction （RT-PCR）.Immunohistochemistry staining was used to assess the expression of SENP1 in 100 NSCLC patients and its association with the clinicopathological features and survival was *** VEGF expression was significantly higher in NSCLC tissues than in normal lung *** of SENP1 by siRNA was associated with decreased VEGF ***1 was over-expressed in 55 of the 100 NSCLC samples （55％） and was associated with a moderate and low histological tumor grade （3.6％,38.2％,and 58.2％ in high,moderate and low differentiated tumors,respectively,P=0.046）,higher T stage （10.9％ in T1,and 89.1％ in T2 and T3 tumor samples,P ＜0.001）and TNM stage （10.9％ in stage Ⅰ,and 89.1％ in stages Ⅱ and Ⅲ tumor samples,P ＜0.001）.The rate of lymph node metastasis was significantly higher in the SENP1 over-expression group （76.4％） than that in the SENP1 low expression group （33.3％,P ＜0.001）.Sixty three patients received postoperative chemotherapy,including 34 with SENP1 over-expression and 29 with SENP1 low *** the 34 patients with SENP1 over-expression,22 （64.7％） patients developed recurrence or metastasis,significantly higher than those in the low expression group 27.6％（8/29）（P=0.005）.Multivariate Cox regression analysis showed that lymph node metastasis （P=0.015）,TNM stage

关键词： small ubiquitin-like modifier proteases 1 (SENP1) non-small cell lung cancer prognosis neoplasm recurrence

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Distribution characteristics of zooplankton biomass in the East China Sea

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Acta Oceanologica Sinica 2004年第2期23卷 337-346页

作者： XUzhaoli CHAOMin chenYaqu EastChinaSeaFisheriesResearchInstitute ChineseAcademyofFisheriesSciencesShanghai200090China KeyandOpenLaboratoryofMarineandEstuarineFisheriesofMinistryofAgricultureofChina Shanghai200090China

On the basis of the data of oceanographic survey in the East China Sea in four seasons during 1997-2000 (23°30'~33°00'N, 118°30'-128°E), the variation of total biomass and diet biomass of zooplankton and their spatial-temporal distribution and relationship with the fishing ground of Engraulis japonicus are approached and analyzed. The results show that the average biomass is 65.32 mg/m3 in four seasons, autumn (86.18 mg/m3) being greater than summer (69.18 mg/m3) greater than spring (55.67 mg/m3) greater than winter (50.33 mg/m3). The average value of diet zooplankton biomass is 40.9 mg/m3. The trends of horizontal distribution both in the total biomass and the diet biomass of zooplankton are similar. The high biomass region (250-500 mg/m3) is very limited, only accounting for 1% of the investigation area. Seasonal variation of the biomass is very remarkable in the west and north parts of East China Sea coastal waters ( 29°30'N,125°E). The horizontal distribution of diet zooplankton depends on the abundance distribution of crustacean. The distribution of diet zooplankton is related to the fishing ground of Engraulis japonicus and the high-density area of young fish and larval. In spring, the central fishing ground of Engraulis japonicus (>100 kg/h) and the high-density area of young fish and larval (>100 individuals per net) are located at the same place of high-density (100-250 mg/m3)area of diet zooplankton in the middle-southern part of East China Sea or the edge of its waters.

关键词： zooplankton total biomass diet biomass East China Sea fishing ground of Engraulis japonicus

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Smoking Cause Specific Lung Cancer—Evidence from Non-Smoking Lung Adenocarcinoma

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Journal of Cancer Therapy 2012年第4期3卷 435-441页

作者： Ning Li Bin Qu Kang Shao zhaoli chen Fengwei Tan Xiaogang Tan Baozhong Li Jiwen Wang Jie He Department of Thoracic Surgery Cancer Hospital and Institute Chinese Academy of Medical Sciences Beijing China

Introduction: Smoking and non-smoking lung cancer have many differences in clinical feature. But those may be the result of interference due to differences in pathological type, as most smoking patients suffer squmous cell lung cancer and non-smokings tend to get adenocarcinoma. This study was conducted on the specific histological type-lung adenocarcinoma-to avoid histological bias and to reveal the true effect of smoking. Methods: A total of 2222 patients with lung adenocarcinoma confirmed by histological or cytological evidence were enrolled from January 1, 1999 to December 31, 2004. Differences in clinical features and prognosis between non-smoking and smoking patients were *** test was used for univariate comparisons. Univariate probability of survival was computed using Kaplan-Meier estimate and compared to using the log-rank test. Cox proportional hazards regression analysis was used to evaluate the risk of death. Results: There were 777 current smokers (34.96%), 197 former smokers (8.87%) and 1248 non-smoking patients (56.17%). 860 non-smoking patients (68.91%) were female, compared with 6.31% among current smokers and 4.06% among former smokers (p < 0.001). Non-smoking patients had an earlier age at diagnosis (p < 0.001) and a better response to chemotherapy (p < 0.001) compared to current smoking patients. Current smoking correlated with lower cell differentiation (p < 0.001) and worse prognosis (p = 0.0024). After multivariate analysis, smoking was identified as an independent negative prognostic factor (HR, 1.302;95% CI, 1.011 - 1.6780, p = 0.041). No difference in prognosis was observed according to smoking conditions in smoking patients. Conclusions: Significent differences exist in clinical features and prognosis between non-smoking and smoking lung adenocarcinoma patients. There is a strong evidence that non-smoking lung adenocarcinoma should be regard as different disease.

关键词： Lung Adenocarcinoma Cigarette Smoke Differentiation Chemotherapy Prognosis

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Upregulation of long noncoding RNA MEG3 Induced by Heat Stress Contributes to Apoptosis of Rat Cardiomyocytes via Promoting TR3 Transcription

Upregulation of long noncoding RNA MEG3 Induced by Heat Stre...

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中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者： Xinxing Wang zhaoli chen Weili Liu Tianhui Wang Ruifeng Duan Yingying Li Lingling Pu Bingnan Deng Environmental Medicine Laboratory Tianjin Institute of Environmental and Operational Medicine

Mitochondria play a key role in cardiomyocytes apoptosis induced by heat *** previous study indicate that TR3 binding to mitochondria and induces apoptosis under ***,the alteration of TR3 responding environmental heat stress is far from fully *** present study,for the first time to the best of our knowledge,discovered that environmental thermal exposure led to MEG3 upregulation,consequently upregulate ELK1 transcription and increasing TR3 transcription,in turn resulting in decline of mitochondria membrane potential(ΔΨm) and apoptosis of ***,MEG3 upregulation was attributed to heat stress-induced c-Myc expression,while ELK1 upregulation was due to the increasing translation of c-Jun induced by ***,TR3 transcription was upregulated via increasing its transcription factor ELK1 the resultant from c-jun upregulation in heat stress ***,we uncover that heat stress exposure results in c-Myc induction and MEG3 expression elevation,further promotes c-Jun translation and in turn initiates ELK1 transcripton,leading to increase of TR3 protein trancription,and decline of mitochondria membrane potential(ΔΨm) as well as apoptosis of *** present study provided a significant insight into understanding the alteration and role of TR3 in heat stress cardiomyocytes.

关键词： TR3 ELK1 c-Myc MPTP Heat Stress

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综合治疗腰椎间盘突出症68例

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中国民族民间医药 2005年第6期14卷 339-341页

作者：陈朝丽富民县人民医院中医科云南富民650400

目的:探讨综合治疗腰椎间盘突出症的临床疗效。方法:采用内服独活寄生汤同时配合腰椎牵引、红外热疗、推拿按摩等措施,综合治疗腰椎间盘突出症,10日为1疗程,疗程间间隔3日开始第2疗程的治疗。结果:68例痊愈47例,占69.1%;好转17例,占25%... 详细信息

目的:探讨综合治疗腰椎间盘突出症的临床疗效。方法:采用内服独活寄生汤同时配合腰椎牵引、红外热疗、推拿按摩等措施,综合治疗腰椎间盘突出症,10日为1疗程,疗程间间隔3日开始第2疗程的治疗。结果:68例痊愈47例,占69.1%;好转17例,占25%;无效4例,占5.9%;总有效率94.1%。结论:内服独活寄生汤,外治配合腰椎牵引、红外热疗、推拿按摩等,综合治疗腰椎间盘突出症,标本兼顾,内外合治,解除肾虚之本,疏通瘀滞经络,可迅速缓解临床症状,减少患者痛苦,疗效显著。

关键词：综合治疗腰椎间盘突出症独活寄生汤

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