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Unresolved Excess Accumulation of Myelin-Derived Cholesterol Contributes to Scar Formation after Spinal Cord Injury

Research 2024年第1期2023卷 17-32页

作者： Bolin Zheng Yijing He Shuai Yin Xu Zhu Qing Zhao Huiyi Yang zhaojie wang Rongrong Zhu Liming Cheng Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration Ministry of EducationDepartment of OrthopedicsTongji HospitalSchool of MedicineTongji UniversityShanghai 200092China Frontier Science Center for Stem Cell Research School of Life Science and TechnologyTongji UniversityShanghai 200092China Clinical Center for Brain and Spinal Cord Research Tongji UniversityShanghai 200092China

Spinal cord injury triggers complex pathological cascades,resulting in destructive tissue damage and incomplete tissue repair.Scar formation is generally considered a barrier for regeneration in the central nervous system.However,the intrinsic mechanism of scar formation after spinal cord injury has not been fully elucidated.Here,we report that excess cholesterol accumulates in phagocytes and is inefficiently removed from spinal cord lesions in young adult mice.Interestingly,we observed that excessive cholesterol also accumulates in injured peripheral nerves but is subsequently removed by reverse cholesteroltransport.Meanwhile,preventing reverse cholesterol transport leads to macrophage accumulation and fibrosis in injured peripheral nerves.Furthermore,the neonatal mouse spinal cord lesions are devoid of myelin-derived lipids and can heal without excess cholesterol accumulation.We found that transplantation of myelin into neonatal lesions disrupts healing with excessive cholesterol accumulation,persistent macrophage activation,and fibrosis.Myelin internalization suppresses macrophage apoptosis mediated by CD5L expression,indicating that myelin-derived cholesterol plays a critical role in impaired wound healing.Taken together,our data suggest that the central nervous system lacks an efficient approach for cholesterol clearance,resulting in excessive accumulation of myelin-derived cholesterol,thereby inducing scar formation after injury.

关键词： healing Spinal impaired

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Detection of ribophagy in yeast and mammals

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Biophysics Reports 2024年第2期10卷 82-101页

作者： Miao Ye Yuting Chen zhaojie Liu Yigang wang Cong Yi Xinyuan Institute of Medicine and Biotechnology School of Life Sciences and Medicine Zhejiang Sci-Tech University Department of Biochemistry and Department of Hepatobiliary and Pancreatic Surgery of the First Affiliated Hospital Zhejiang University School of Medicine Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation Affiliated Cancer Hospital & Institute of Guangzhou Medical University School of Basic Medical Sciences Guangzhou Medical University

Ribophagy, the cellular process wherein ribosomes are selectively self-digested through autophagy,plays a pivotal role in maintaining ribosome turnover. Understanding the molecular regulatory mechanisms governing ribophagy is pivotal to uncover its significance. Consequently, the establishment of methods for detecting ribophagy becomes important. In this protocol, we have optimized, enriched,and advanced existing ribophagy detection techniques, including immunoblotting, fluorescence microscopy, and transmission electron microscopy(TEM), to precisely monitor and quantify ribophagic events. Particularly noteworthy is the introduction of TEM technology for yeast ribophagy detection. In summary, the delineated methods are applicable for detecting ribophagy in both yeast and mammals,laying a solid foundation for further exploring the physiological importance of ribophagy and its potential implications in diverse cellular environments.

关键词： Ribophagy Ribosome turnover Immunoblotting Fluorescence microscopy Transmission electron microscopy Yeast and mammals

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FSS: algorithm and neural network accelerator for style transfer

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Science China(Information Sciences) 2024年第2期67卷 253-266页

作者： Yi LING Yujie HUANG Yujie CAI zhaojie LI Mingyu wang Wenhong LI Xiaoyang ZENG State Key Laboratory of ASIC & System Fudan University Shanghai Explore X Technology Co. Ltd.

Neural networks(NNs), owing to their impressive performance, have gradually begun to dominate multimedia processing. For resource-constrained and energy-sensitive mobile devices, an efficient NN accelerator is necessary. Style transfer is an important multimedia application. However, existing arbitrary style transfer networks are complex and not well supported by current NN accelerators, limiting their application on mobile devices. Moreover, the quality of style transfer needs improvement. Thus, we design the Fast Style system(FSS), where a novel algorithm and an NN accelerator are proposed for style transfer. In FSS, we first propose a novel arbitrary style transfer algorithm, Fast Style. We propose a light network that contributes to high quality and low computational complexity and a prior mechanism to avoid retraining when the style changes. Then, we redesign an NN accelerator for Fast Style by applying two improvements to the basic NVIDIA deep learning accelerator(NVDLA) architecture. First, a flexible dat FSM and wt FSM are redesigned to enable the original data path to perform other operations(including the GRAM operation)by software programming. Moreover, statistics and judgment logic are designed to utilize the continuity of a video stream and remove the data dependency in the instance normalization, which improves the accelerator performance by 18.6%. The experimental results demonstrate that the proposed Fast Style can achieve higher quality with a lower computational cost, making it more suitable for mobile devices. The proposed NN accelerator is implemented on the Xilinx VCU118 FPGA under a 180-MHz clock. Experimental results show that the accelerator can stylize 512×512-pixel video with 20 FPS, and the measured performance reaches up to 306.07 GOPS. The ASIC implementation in TSMC 28 nm achieves about 22 FPS in the case of a 720-p video.

关键词： neural network accelerator style transfer neural network deep learning

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On-chain is not enough:Ensuring pre-data on the chain credibility for blockchain-based source-tracing systems

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Digital Communications and Networks 2023年第5期9卷 1053-1060页

作者： Yilei wang zhaojie wang Guoyu Yang Shan Ai Xiaoyu Xiang Chang Chen Minghao Zhao School of Computer Science Qufu Normal UniversityRizhao276826China Guangxi Key Laboratory of Cryptography and Information Security Guilin University of Electronic TechnologyGuilin541004China Institute of Artificial Intelligence and Blockchain Guangzhou UniversityGuangzhou510006China School of Software Tsinghua UniversityTsinghua UniversityBeijing100191China

The blockchain provides a reliable and scalable method for enabling source-tracing functionality in large-scale Internet of Things(IoT)systems.Traditional blockchain-based source tracing applications are generally based on the hypothesis that the raw data collected by each IoT node are credible and consistent,which however may not always be the truth.As no mechanism ensures the reliability of the original data collected from the IoT devices,these data may be accidently screwed up or maliciously tampered with before they are uploaded on-chain.To address this issue,we propose the Multi-dimensional Certificates of Origin(MCO)method to filter out the potentially incredible data-till all the data uploaded to the chain are credible.To achieve this,we devise the Multidimensional Information Cross-Verification(MICV)and Multi-source Data Matching Calculation(MDMC)methods.MICV verifies whether a to-be-uploaded datum is consistent or credible,and MDMC determines which data should be discarded and which data should be kept to retain the most likely credible/untampered ones in the circumstance when data inconsistency appears.Large-scale experiments show that our scheme ensures on the credibility of data and off the chain with an affordable overhead.

关键词： Blockchain Traceability Blockchain security

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Ion elemental-optimized layered double hydroxide nanoparticles promote chondrogenic differentiation and intervertebral disc regeneration of mesenchymal stem cells through focal adhesion signaling pathway

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Bioactive Materials 2023年第4期22卷 75-90页

作者： zhaojie wang Huiyi Yang Xu Xu Hongxing Hu Yuxin Bai Jian Hai Liming Cheng Rongrong Zhu Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education Department of OrthopedicsTongji Hospital Affiliated to Tongji UniversitySchool of Life Science and TechnologyTongji UniversityShanghai200065China Frontier Science Center for Stem Cell Research Tongji UniversityShanghai200065China

Chronic low back pain and dyskinesia caused by intervertebral disc degeneration(IDD)are seriously aggravated and become more prevalent with age.Current clinical treatments do not restore the biological structure and inherent function of the disc.The emergence of tissue engineering and regenerative medicine has provided new insights into the treatment of IDD.We synthesized biocompatible layered double hydroxide(LDH)nanoparticles and optimized their ion elemental compositions to promote chondrogenic differentiation of human umbilical cord mesenchymal stem cells(hUC-MSCs).The chondrogenic differentiation of LDH-treated MSCs was validated using Alcian blue staining,qPCR,and immunofluorescence analyses.LDH-pretreated hUC-MSCs were differentiated prior to transplantation into the degenerative site of a needle puncture IDD rat model.Repair and regeneration evaluated using X-ray,magnetic resonance imaging,and tissue immunostaining 4-12 weeks after transplantation showed recovery of the disc space height and integrated tissue structure.Transcriptome sequencing revealed significant regulatory roles of the extracellular matrix(ECM)and integrin receptors of focal adhesion signaling pathway in enhancing chondrogenic differentiation and thus prompting tissue regeneration.The construction of ion-specific LDH nanomaterials for in situ intervertebral disc regeneration through the focal adhesion signaling pathway provides theoretical basis for clinical transformation in IDD treatment.

关键词： Mesenchymal stem cells Layered double hydroxide Chondrogenic differentiation Intervertebral disc degeneration Focal adhesion signaling pathway

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Dynamic immune recovery process after liver transplantation revealed by single-cell multi-omics analysis

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The Innovation 2024年第3期5卷 55-68页

作者： Rui wang Xiao Peng Yixin Yuan Baojie Shi Yuan Liu Hengxiao Ni Wenzhi Guo Qiwei Yang Pingguo Liu Jie wang zhaojie Su Shengnan Yu Dehua Liu Jinyan Zhang Junjie Xia Xueni Liu Hao Li Zhengfeng Yang Zhihai Peng Organ Transplantation Clinical Medical Center of Xiamen University Department of General SurgeryXiang’an Hospital of Xiamen UniversitySchool of MedicineXiamen UniversityXiamen 361102China Organ Transplantation Institute of Xiamen University Xiamen Human Organ Transplantation Quality Control CenterXiamen Key Laboratory of Regeneration MedicineFujian Provincial Key Laboratory of Organ and Tissue RegenerationSchool of MedicineXiamen UniversityXiamen 361102China Department of General Surgery Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China State Key Laboratory of Cellular Stress Biology School of Life SciencesFaculty of Medicine and Life SciencesXiamen UniversityXiamen 361102China Department of Hepatobiliary and Pancreatic Surgery The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450000China Department of Hepatobiliary&Pancreatic Surgery The National Key Clinical SpecialtyZhongshan Hospital of Xiamen UniversitySchool of MedicineXiamen UniversityXiamen 361102China Precision Research Center for Refractory Diseases Institute for Clinical ResearchShanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 201620China

Elucidating the temporal process of immune remodeling under immunosuppressive treatment after liver transplantation(LT)is critical for precise clinical management strategies.Here,we performed a single-cell multi-omics analysis of peripheral blood mononuclear cells(PBMCs)collected from LT patients(with and without acute cellular rejection[ACR])at 13 time points.Validation was performed in two independent cohorts with additional LT patients and healthy controls.Our study revealed a four-phase recovery process after LT and delineated changes in immune cell composition,expression programs,and interactions along this process.The intensity of the immune response differs between the ACR and non-ACR patients.Notably,the newly identified inflamed NK cells,CD14+RNASE2+monocytes,and FOS-expressing monocytes emerged as predictive indicators of ACR.This study illuminates the longitudinal evolution of the immune cell landscape under tacrolimus-based immunosuppressive treatment during LT recovery,providing a four-phase framework that aids the clinical management of LT patients.

关键词： process analysis precise

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Spinal cord injury:molecular mechanisms and therapeutic interventions

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Signal Transduction and Targeted Therapy 2023年第7期8卷 3346-3373页

作者： Xiao Hu Wei Xu Yilong Ren zhaojie wang Xiaolie He Runzhi Huang Bei Ma Jingwei Zhao Rongrong Zhu Liming Cheng Division of Spine Department of OrthopaedicsTongji HospitalTongji University School of Medicine200065 ShanghaiChina Key Laboratory of Spine and Spinal cord Injury Repair and Regeneration(Tongji University) Ministry of Education200065 ShanghaiChina Clinical Center For Brain And Spinal Cord Research Tongji University200065 ShanghaiChina

Spinal cord injury(SCI)remains a severe condition with an extremely high disability rate.The challenges of SCI repair include its complex pathological mechanisms and the difficulties of neural regeneration in the central nervous system.In the past few decades,researchers have attempted to completely elucidate the pathological mechanism of SCI and identify effective strategies to promote axon regeneration and neural circuit remodeling.

关键词： Spinal injury mechanisms

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Exploration on Management Measures of Onion Production in Eastern Henan Province

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Agricultural Biotechnology 2024年第2期13卷 5-7页

作者： zhaojie XIAO Shangqiu Academy of Agriculture and Forestry Sciences Shangqiu 476000China

In this paper, the management points and cultivation difficulties of onion in different stages were discussed in detail from the aspects of onion cultivation seasons, cultivation methods, sowing and seedling raising, proper planting, field water and fertilizer management, stalk removal period, pest control, timely harvest and so on. This paper provides theoretical guidance for the high quality and high yield of onion in Huang-Huai area and data reference for further improvement of high-yielding onion cultivation technique system.

关键词： Onion Cultivation Huang-Huai area

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Selectivity switching between CO and formate for CO_(2) reduction on Sb modified amorphous ZnO by electronic effect

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Nano Research 2023年第10期16卷 12144-12152页

作者： Hongyu Chen Shoufu Cao Lu wang Xiaojing Lin Qiuying Zhu Yizhu Shang Shuxian Wei Siyuan Liu zhaojie wang Baojun Wei Xiaoqing Lu College of Science China University of PetroleumQingdao 266580China School of Materials Science and Engineering China University of PetroleumQingdao 266580China

The adjustable intermediate binding capacity in electrocatalytic carbon dioxide(CO_(2))reduction is critical for varying the reaction pathways to desired products.Herein,we first report the synthesis of boron-doped amorphous zinc oxide with(B-a-ZnO-Sb)or without antimony nanoparticles embedding(B-a-ZnO)via one-step wet chemical method,which is easy to scale up by enlarging the vessel and increasing feeding.Sb successfully realizes the product switching from CO on B-a-ZnO to formate on B-a-ZnOSb.Both experimental and theoretical results reveal that Sb weakens the charge interaction on Zn atoms.Based on the moderate adsorption of*COOH and strong adsorption of*OCHO and*HCOOH for B-a-ZnO,the foreign Sb weakens the adsorption of these intermediates and brings about a favor formate production instead of CO.This work points out a new direction for the synthesis of amorphous ZnO-based catalysts and provides advanced insights into the aimed selectivity switch for CO_(2)reduction by electronic effect.

关键词： carbon dioxide reduction amorphous ZnO electronic effect selectivity switching

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How can the Dual-atom Catalyst FeCo-NC Surpass Singleatom Catalysts Fe-NC/Co-NC in CO_(2) RR?-CO Intermediate Assisted Promotion via a Synergistic Effect

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Energy & Environmental Materials 2023年第1期6卷 300-308页

作者： Shoufu Cao Sainan Zhou Hongyu Chen Shuxian Wei Siyuan Liu Xiaojing Lin Xiaodong Chen zhaojie wang Wenyue Guo Xiaoqing Lu School of Materials Science and Engineering China University of PetroleumQingdao Shandong 266580China College of Science China University of PetroleumQingdao Shandong 266580China

Atomically dispersed catalysts are widely adopted in CO_(2)reduction reaction(CO_(2)RR)due to maximal atomic utilization and high catalytic activity.Dual-atom catalysts(DACs),with more dispersed active sites and distinct electronic structures compared with single-atom catalysts(SACs),may exhibit diverse catalytic performance.Herein,the DAC FeCo-NC and SAC Fe-NC/Co-NC are employed as probes to explore DACs advantage in CO_(2)RR.Results show that the moderate interaction between the dual-atom center and N coordination balances structural stability and catalytic activity.CO is the only product on Fe-NC/Co-NC,and the high limiting potentials from−1.22 to−1.67 V inhibit further reduction.FeCo-NC assisted with CO intermediate exhibits low limiting potentials of−0.64 V for both CH_(3)OH and CH 4,comparable to those on Cu-based catalysts.Under circumstance of applied potentials,CO_(2)RR on FeCo-NC has greater advantages in yielding CH_(3)OH and CH 4 than that on Fe-NC/Co-NC,and hydrogen evolution reaction is severely inhibited.The intrinsic essence is that dual-atom center can provide large spin-polarization and multi-electron transfer capability,rendering CO intermediates as effective electronic and geometric modifiers in CO_(2)RR.This work highlights FeCo-NC as a high-performance CO_(2)RR catalyst toward deep-reduction C1 products and elucidates CO intermediate assisted promotion mechanism via a dual-atom synergistic effect.

关键词： dispersed limiting rendering

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