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Journal of Oceanology and Limnology 2021年第5期39卷 1910-1925页

作者： Lizhou LIN Haifeng GU zhaohe luo Na WANG Department 1 Third Institute of OceanographyMinistry of NaturalResourcesXiamen 361005China

Pico-and nano-eukaryotic plankton are important members of marine *** understand their community structure and spatio-temporal variations in the Beibu Gulf,and to study the impact of Changjiang Nuclear Power Plant(CNPP)located in the northwest coast of the Hainan Island on the eukaryotic plankton,we studied these issues in the coastal water near CNPP bimonthly from February 2018 to January 2019 via 18 S Illumina *** results reveal a higher genetic alphadiversity and less beta-diversity of the plankton community in small-size(0.45-5μm and 5-20μm)fractions than in large-size(20-200μm and>20μm)*** small-sized phytoplankton,including Micromonas(Chlorophyta)and Minutocellus(Bacillariophyta),as well as heterotrophic plankton,including Paraphysomonas(Chrysophyceae),Ellobiopsis(Dinoflagellates),and Syndiniales(Dinoflagellates),took up a considerable proportion in eukaryotic plankton *** addition,it showed a slight temperature increase and no significant difference in eukaryotic plankton community between the outfall of CNPP and other sampling sites,suggesting that the impact of the thermal discharge from the CNPP on local eukaryotic plankton community was limited.

关键词： nuclear power plant the Beibu Gulf eukaryotic plankton community high-throughput sequencing picoplankton nanoplankton

Predicting Ecological Distribution of the Toxic Dinoflagellate Alexandrium minutum in China Sea Using Ecological Niche Modeling

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Journal of Ocean University of China 2023年第4期22卷 1119-1128页

作者： WANG Changyou ZHENG Ping GU Haifeng luo zhaohe luo Zhuhua MAO Longjiang ZHANG Yuanzhi School of Marine Sciences Nanjing University of Information Science and TechnologyNanjing 210044China Third Institute of Oceanography Ministry of Natural ResourcesXiamen 361005China Key Laboratory of Marine Biogenetic Resources Third Institute of OceanographyMinistry of Natural ResourcesXiamen 361005China

Alexandrium minutum from the China Sea produces a range of toxins and causes damage to the local ecosystems and *** is essential to understand environmental factors affecting potential *** distributions of *** in the China Sea were predicted based on maximum entropy modeling,and dominant environmental variables were studied through analyses of variable contributions and response *** results showed that highly suitable areas were mainly located in the southwest of the Yellow Sea,the Laizhou Bay,and north of Haizhou *** coast of the South China Sea was predicted as a low-suitability area,and the coast of the East China Sea as an unsuitable *** temperature of the coldest month(T_min)had the largest drop in permutation importance but a low percent *** probability of presence of *** increased with increasing concentration of nitrate(NO3−)and annual mean temperature(T_ann)over a wide range of *** response curves decreased with increasing concentration of phosphate(PO43−)and ratio of NO_(3)^(−)to PO_(4)^(3−)(N_P_ratio)when PO_(4)^(3)−is above 0.049μmolL^(-1) and N_P_ratio above 4,indicating that low values of PO_(4)^(3−) concentration and N_P_ratio favour the occurrence of *** a predictor,the variance of annual temperature(T_Var)had the highest percent contribution and ***_(4)^(3−) was predicted to have much more information than the other variables,and exhibited the second largest drop in permutation importance and percent *** T_Var and PO_(4)^(3−) are the most important dominant predictor variables.

关键词： Alexandrium minutum MaxEnt,habitat suitability environmental variable potential distributions

Characterization of Amphidinium(Amphidiniales,Dinophyceae)species from the China Sea based on morphological,molecular,and pigment data

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Journal of Oceanology and Limnology 2022年第3期40卷 1191-1219页

作者： zhaohe luo Hua ZHANG Qun LI Lei WANG Hala F MOHAMED Songhui LÜ Haifeng GU Third Institute of Oceanography Ministry of Natural ResourcesXiamen 361005China Research Center of Harmful Algae and Marine Biology College of Life Science and TechnologyJinan UniversityGuangzhou 510362China Shenzhen Academy of Environmental Science Shenzhen 510008China Botany&Microbiology Department Faculty of ScienceAl-Azhar University(Girls Branch)Cairo 11751Egypt Southern Marine Science and Engineering Guangdong Laboratory Zhuhai 519000China Observation and Research Station of Coastal Wetland Ecosystem in Beibu Gulf Ministry of Natural ResourcesBeihai 536015China

Amphidinium species are amongst the most abundant benthic dinoflagellates in marine intertidal sandy *** of them are able to produce a variety of bioactive compounds that can have both harmful effects and pharmaceutical *** diversity of Amphidinium in shallow waters along the Chinese coast was investigated by isolating single cells from sand,coral,and macroalgal samples collected from 2012 to *** morphologies were subjected to examination using light microscopy(LM)and scanning electron microscopy(SEM).A total of 74 Amphidinium strains were morphologically identified,belonging to 11 species:***,***,***,***,***,***,***,***,***,***,***,as well as an undefined *** last seven species have not been previously reported in Chinese *** carterae subcladesⅠ,Ⅱ,andⅣwere found in the South China Sea,while subcladeⅢwas only found in the Yellow *** body scales were observed on the surface of subcladesⅢ and Ⅳ,supporting the idea that *** might contain several different *** subunit ribosomal RNA(LSU rRNA)sequencesbased phylogeny revealed two groups(GroupsⅠandⅡ)within Amphidinium,which is consistent with the relative position of sulcus(in touch with cingulum or not).In addition,large differences in morphology and molecular phylogeny between ***(the type species of Amphidinium)and other species,suggest that a subdivision of Amphidinium might be *** pigment profiles of all available strains were analyzed by high performance liquid chromatography(HPLC).Eleven pigments,including peridinin,diadinoxanthin,diatoxanthin,pheophorbide(and pheophorbide a),antheraxanthin,β-carotene,and four different chlorophylls were *** high pheophorbide/pheophorbide a ratio in Amphidinium implies that it may be a good candidate as a natural source of photosensitizers,a well-known anticancer drug.

关键词： harmful algae benthic-epiphytic dinofl agellate phylogeny geographic distribution pheophorbide photosensitizers

Morphological and molecular characterization of Tovellia ***(Dinophyceae) from Jiulong River,China

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Morphological and molecular characterization of Tovellia ***...

福建省海洋学会2016年学术年会

作者： zhaohe luo Xuejing You Kenneth Neil Mertens Haifeng Gu Third Institute of Oceanography SOA College of Life Science and Technology Jinan University Fujian Normal University

The dinoflagellate genus Tovellia is exclusively freshwater and characterized by the presence of an extraplastidial eyespot(Type C sensu Moestrup&Daugbjerg),a straight apical line of narrow plates(ALP)and a bipolar cyst with axial *** we establish a Ibvellia strain TSJL01 through incubation of a cyst with a pronounced paracingulum and bearing long,hollow and tapering processes collected from Jiulong River(China).The morphology of motile cells and cysts was investigated using light microscopy,scanning electron microscopy and transmission electron *** of strain TSJL01 possess a long ALP on the apex,with 10 or 11 narrow elongated vesicles lined at each *** amphiesma has many pentagonal or hexagonal latitudinal vesicles,with7-10 series on the epicone,4—5 on the hypocone and 2 in the *** sections reveal a type C eyespot,and numerous chloroplasts radiating from a central pyrenoid *** produced in culture differ from those observed in the field in the shape of their *** analyses based on large subunit ribosomal DNA(LSU rDNA)support that TSJL01 is nested within the genus Tovellia,and is closely related to *** Chinese strain differs from *** mainly in the number of elongated plates surrounding ALP and thus was tentatively identified as *** closely related species,Gymnodinium dodgei,is transferred to Ibvellia based on the eyespot and cyst morphology.

关键词： Cyst freshwater dinoflagellate Gymnodinium dodgei type C eyespot Woloszynskia

Morphology,ultrastructure,and phylogeny of Protodinium simplex and Biecheleriopsis ***(Dinophyceae) from the China Sea

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Morphology,ultrastructure,and phylogeny of Protodinium simpl...

福建省海洋学会2016年学术年会

作者： zhaohe luo Weidong Yang Bin Xu Bingxin Zheng Haifeng Gu College of Life Science and Technology Jinan University Third Institute of Oceanography Institute of Urban Environment Chinese Academy of Sciences

The presence of the genera Protodinium and Biecheleriopsis in the China Sea has not been reported *** the present study,three strains of Protodinium simplex and eleven strains oiBiecheleriopsis *** were established from the China Sea and subjected to detailed examinations using light microscopy,scanning electron microscopy,and transmission electron *** simplex possessed an elongated apical vesicle(EAV) on the apex,and the epicone,cingulum,and hypocone each contained three latitudinal series of *** sections revealed that *** possessed a type E eyespot,thus supporting its classification within the family *** *** was morphologically identical to ***,as it possessed an EAV and 4,3,and 3 latitudinal series of vesicles on the epicone,cingulum,and hypocone,respectively,but it lacked swollen ends of thylakoids in the pyrenoids and ***,Protodinium and Biecheleriopsis were nearly indistinguishable,separated only by the number of EAV *** analyses based on large subunit ribosomal DNA(LSU rDNA) and internal transcribed spacer region(ITS) sequences confirmed their close relationship.

关键词： Biecheleriopsis adriatica Gymnodinium simplex molecular phylogeny Protodinium simplex type E eyespot

Peridiniopsis jiulongensis,a new freshwater dinotlagellate with a diatom endosymbiont from China

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Peridiniopsis jiulongensis,a new freshwater dinotlagellate w...

福建省海洋学会2016年学术年会

作者： Xuejing You zhaohe luo Yuping Su Li Gu Haifeng Gu Fujian Normal University Third Institute of Oceanography

Detailed study on Peridiniopsis species using modem techniques is still *** we report a new Peridiniopsis species isolated from the Jiulong River,China,which was described as *** *** *** cells of *** were subjected to detailed examination by light microscopy,scanning electron microscopy and transmission electron *** has a plate pattern of Po,X,3′,1a,6″,5C,5S,5′″,2″″ and harbors a dinokaryotic and a eukaryotic *** eyespot of *** was bounded by triple envelopes and thus classified as type *** phytogeny based on dinoflagellate small subunit ribosomal DNA(SSU rDNA) sequences revealed that dinoflagellates with a diatom endosymbiont were monophyletic and *** was grouped together with Peridiniopsis ***,*** and *** phylogeny based on endosymbiont SSU rDNA sequences showed that ***,***,and *** formed a well resolved group and were close to Thalassiosira species.

关键词： cyst Jiulong River Peridiniopsis P.kevei P.niei P.penardii

Morphology,molecular phylogeny and azaspiracid profile of Azadimium poporum(Dinophyceae) from the Gulf of Mexico

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Morphology,molecular phylogeny and azaspiracid profile of Az...

福建省海洋学会2016年学术年会

作者： zhaohe luo Bernd Krock Kenneth Neil Mertens Andrea Michelle Price Robert Eugene Turner Nancy N.Rabalais Haifeng Gu College of Science and technology Jinan University Third institute of Oceanography SOA Alfred Wegener institute for Potar and Marine Research Research Unit for Palaeontology Ghent University Department Gengraphy McGiill University Deparfment of Oceanography and Coastal Science Louisiana State University

Azadinium poporum produces a variety of azaspiracids and consists of several ribotypes,but information on its biogeography is limited.A strain of A poponim(GM29) was incubated from a Gulf of Mexico sediment *** GM29 was characterized by a plate pattern of po,cp,x,4′,3a,6″,6C,5S,6′″,2″″,a distinct ventral pore at the junction of po and the first two apical plates,and a lack of an antapical spine,thus fitting the original description of A *** genus Azadinium has not been reported in waters of the United States of America before this *** phylogeny,based on large subunit ribosomal DNA(LSU rDNA) and internal transcribed spacer(ITS) sequences,reveals that strain GM29 is nested within the well-resolved A poponim complex,but forms a sister clade either to ribotype B(ITS) or ribotype C(LSU).It is,therefore,designated as a new ribotype,termed as ribotype *** and ITS sequences similarity among different ribotypes of A poponim ranges from 95.4%to 98.2%.and from97.1%to 99.2%respectively,suggesting that the LSU fragment is a better candidate for molecular *** profiles were analyzed using LC-MS/MSand demonstrate that strain GM29 produces predominantly AZA-2 with an amount of 45 fg/*** results suggest that A popomm has a wide distribution and highlights the risk potential of azaspiracid intoxication in the United States.

关键词： AZA-2 AZA-2 phosphate Biogeography LC-MS/MS LSU rDNA Ribotype D

Cyst-theca relationship and phylogenetic positions of Scrippsiella plana *** ***(Peridiniales,Dinophyceae)

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Cyst-theca relationship and phylogenetic positions of Scripp...

福建省海洋学会2016年学术年会

作者： zhaohe luo KENNETH NEIL MERTENS SIAMAK BAGHERI HILAL AYDIN YOSHIHITO TAKANO KAZUMI MATSUOKA FRANCINE M.G. MCCARTHY HAIFENG GU Third Institute of Oceanography SOA Research Unit Palaeontology Ghent University

Species belonging to the dinophyte genus ScrippsieUa are frequently reported in marine waters,but information on their distribution in brackish environments is *** we describe a new species,***,through incubation of non-calcified cysts from sediments collected in the South China Sea and Caspian *** vegetative cells consist of a conical epitheca and a rounded hypotheca with the plate formula of Po,X,4′,3a,7″,5C+t,5S,5′″,2″″.It differs from other ScrippsieUa species by its flattened body in dorsoventral view and a small first anterior intercalary(la) plate(half the size of plate 3a).ScrippsieUa plana strains from the South China Sea and Caspian Sea share identical internal transcribed spacer(ITS) sequences,and show phenotypic plasticity and local adaptation in growth rate at various salinities,consistent with the environments in which they *** addition,two strains of *** were obtained by mcubating ellipsoid cysts with calcareous spines from sediments collected along the Turkish and Hawaiian *** also share identical ITS sequences and differ from Duboscquodinium collinii(a parasite of tintinnids) only at two base pair positions(in the ITS2 region).Molecular phybgeny based on ITS and large subunit ribosomal DNA(LSU rDNA) sequences revealed that *** was nested within the Calaodinellum(CAL) clade and *** within the ***(STR) *** phylogenetic position of ’Peridinium’wisconsinense is reported for the first time,which supports multiple transitions of the Peridiniales to freshwater.

关键词： calcareous dinoflagellates cysts Duboscquodinium collinii growth ITS Peridinium wisconsinense

中国近海甲藻环沟藻属2个新记录种:纺锤环沟藻和莫氏环沟藻

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生物多样性 2014年第3期22卷 401-406页

作者：顾海峰罗肇河刘丽丽高越国家海洋局第三海洋研究所福建厦门361005 厦门市海洋与渔业研究所福建厦门361005 厦门大学近海海洋环境科学国家重点实验室福建厦门361005

甲藻环沟藻属于一类无色素体、表面有脊的裸甲藻,因可捕食一些重要的赤潮生物而在海洋生态系统中扮演着重要的角色。有关中国近海环沟藻属的物种多样性信息非常有限。本文报道了2个新记录种——纺锤环沟藻(Gyrodinium fusiforme)和莫氏... 详细信息

甲藻环沟藻属于一类无色素体、表面有脊的裸甲藻,因可捕食一些重要的赤潮生物而在海洋生态系统中扮演着重要的角色。有关中国近海环沟藻属的物种多样性信息非常有限。本文报道了2个新记录种——纺锤环沟藻(Gyrodinium fusiforme)和莫氏环沟藻(***)。纺锤环沟藻细胞呈纺锤形,长48.0–58.0μm,宽18.0–23.0μm,长宽比为2.4–3.0,和模式种相比体型和长宽比都较小。莫氏环沟藻细胞也呈纺锤形,长约30μm,宽约15μm。我们测定了纺锤环沟藻和莫氏环沟藻大亚基的部分序列,并根据大亚基序列利用最大似然法和贝叶斯法建立了系统发育树。结果显示环沟藻属是单源的,纺锤环沟藻和裂缝环沟藻(***)聚合在一起,但是与螺旋环沟藻(***)分离。纺锤环沟藻和莫氏环沟藻分别可以摄食米氏凯伦藻(Karenia mikimotoi)和具齿原甲藻(Prorocentrum dentatum),前者在米氏凯伦藻赤潮中的大量出现显示它可以促进赤潮的消退。

关键词： Gyrodinium fusiforme Gyrodinium-moestrupii Karenia mikimotoi 大亚基赤潮

兔自体真皮成纤维细胞对增生性瘢痕作用的实验研究

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中华整形外科杂志 2018年第9期34卷 758-768页

作者：杨晓双胡鹏王达利魏在荣冀玫宏罗昭和遵义医学院附属医院烧伤整形外科 563000

目的探讨兔耳瘢痕形成早期瘢痕内局部移植自体真皮成纤维细胞（Fbs）对增生性瘢痕形成及创面愈合质量的影响,以探讨Fbs用于增生性瘢痕的防治的可行性.方法运用机械法联合酶消化法从新西兰大白兔背部正中正常皮肤组织中提取真皮Fbs,取... 详细信息

目的探讨兔耳瘢痕形成早期瘢痕内局部移植自体真皮成纤维细胞（Fbs）对增生性瘢痕形成及创面愈合质量的影响,以探讨Fbs用于增生性瘢痕的防治的可行性.方法运用机械法联合酶消化法从新西兰大白兔背部正中正常皮肤组织中提取真皮Fbs,取第3代生长良好的细胞,向成骨细胞、成脂肪细胞诱导分化.取2只实验兔作为预实验观察对象（即未干预组）,于兔双耳腹侧手术形成全层皮肤缺损创面,创面不做任何处理,待其自然愈合,记录完全上皮化时间及增生性瘢痕形成的情况;另取6只实验用兔设立兔左耳为实验组,右耳为对照组,于创面上皮化后第2天（术后20 d）、术后30 d（瘢痕增生最明显时）,采用体外培养的经BrdU标记后的第4代自体真皮Fbs于实验组创面及增生性瘢痕周围的皮下至软骨上层之间进行环形注射,对照组在上述相应时间点注射相同剂量的生理盐水.第2次细胞移植后7d（术后37 d左右）切取增生性瘢痕组织,记录3组（未干预组、实验组、对照组）增生性瘢痕的大体外观及其组织学变化;实验组术后37 d增生性瘢痕组织行免疫荧光染色对移植细胞进行示踪;实时荧光-多聚合酶链反应（Real Time-PCR,RT-PCR）检测对照组及实验组术后37 d增生性瘢痕组织中转化生长因子-β1（Transforming growth factor-β1,TGF-β1）和核心蛋白多糖（decorn,DCN）的mRNA表达变化情况,酶联免疫吸附法（ELISA）检测增生性瘢痕组织中DCN、TGF-β1、Ⅰ型胶原及Ⅲ型胶原的蛋白表达情况.结果术后37 d实验组瘢痕较对照组平、软,色泽轻度变浅,体积缩小.组织学结果提示实验组与对照组相比真皮浅层Fbs、炎性细胞数量减少,结缔组织的增生及胶原沉积减少,基底层细胞、胶原纤维排列整齐;RT-PCR结果表明,实验组增生性瘢痕组织中TGF-β1 mRNA表达量较对照组明显降低,DCN mRNA表达量明显增高,差异均有统计学意义（P〈0.01）.ELISA结果表明,实验组增生性瘢痕组织中TGF-β1、Ⅰ、Ⅲ型胶原含量较对照组均显著降低,DCN含量显著增高,差异均有统计学意义（P〈0.01）.结论兔背部真皮Fbs对兔耳增生性瘢痕有明显抑制作用,其机制与促进增生性瘢痕局部组织中DCN表达的上调及TGF-β1表达的下调,减少Ⅰ型、Ⅲ型胶原蛋白的合成及细胞外基质的沉积,促进胶原的有序排列等作用有关.

关键词：增生性瘢痕成纤维细胞细胞移植兔耳瘢痕模型