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Blocking NF-kB nuclear translocation leads to p53-related autophagy activation and cell apoptosis

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World Journal of Gastroenterology 2011年第4期17卷 478-487页

作者： Bao-Song Zhu Chun-Gen xing fang Lin xiao-Qing fan Kui zhao Zheng-Hong Qin Department ofGeneral Surgery The Second Affiliated Hospital Soochow University Suzhou 215006 Jiangsu Province China Laboratory of Aging and Nervous Diseases Soochow University School of Medicine Suzhou215123 Jiangsu Province China Department of General Surgery The Second Hospital Of Jiaxing City Jiaxing 314000 Jiangsu ProvinceChina

AIM: To investigate the anti-tumor effects of nuclear factor-κB (NF-κB) inhibitor SN50 and related mechanisms of SGC7901 human gastric carcinoma cells. METHODS: MTT assay was used to determine the cytotoxic effects of SN50 in gastric cancer cell line SGC7901. Hoechst 33258 staining was used to detect apoptosis morphological changes after SN50 treatment. Activation of autophagy was monitored with monodansylcadaverine (MDC) staining after SN50 *** staining was used to detect the expression of light chain 3 (LC3). Mitochondrial membrane potential was measured using the fluorescent probe JC-1. Western blotting analysis were used to determine the expression of proteins involved in apoptosis and autophagy including p53, p53 upregulated modulator of apoptosis (PUMA), damage-regulated autophagy modulator (DRAM), LC3 and Beclin 1. We detected the effects of p53-mediated autophagy activation on the apoptosis of SGC7901 cells with the p53 inhibitor pifithrin-α. RESULTS: The viability of SGC7901 cells was inhibited after SN50 treatment. Inductions in the expression of apoptotic protein p53 and PUMA as well as autophagic protein DRAM, LC3 and Beclin 1 were detected with Western blotting analysis. SN50-treated cells exhibited punctuate microtubule-associated protein 1 LC3 in immunoreactivity and MDC-labeled vesicles increased after treatment of SN50 by MDC staining. Collapse of mitochondrial membrane potential Δψ were detected for 6 to 24 h after SN50 treatment. SN50-induced increases in PUMA, DRAM, LC3 and Beclin 1 and cell death were blocked by the p53 specific inhibitor pifithrin-α. CONCLUSION: The anti-tumor activity of NF-κB inhibitors is associated with p53-mediated activation of autophagy.

关键词： Nuclear factor-κB SN50 Autophagy P53 Cell apoptosis

Semisupervised learning-based depth estimation with semantic inference guidance

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Science China(Technological Sciences) 2022年第5期65卷 1098-1106页

作者： ZHANG Yan fan XiaoPeng zhao DeBin Department of Computer Science and Technology Harbin Institute of TechnologyHarbin150001China

Depth estimation is a fundamental computer vision problem that infers three-dimensional(3D)structures from a given *** it is an ill-posed problem,to fit the projection function from the given scene to the 3D structure,traditional methods generally require mass amounts of annotated *** pixel-level annotation is quite labor consuming,especially when addressing reflective surfaces such as mirrors or *** widespread application of deep learning further intensifies the demand for large amounts of annotated ***,it is urgent and necessary to propose a framework that is able to reduce the requirement on the amount of *** this paper,we propose a novel semisupervised learning framework to infer the 3D structure from the given ***,semantic information is employed to make the depth inference more ***,we make both the depth estimation and semantic segmentation coarse-to-fine frameworks;thus,the depth estimation can be gradually guided by semantic *** compare our model with state-of-the-art *** experimental results demonstrate that our method is better than many supervised learning-based methods,which proves the effectiveness of the proposed method.

关键词： depth estimation semisupervised learning semantic information neural networks

Numerical Study on the Vertical Water Exit of A Cylinder with Cavity

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China Ocean Engineering 2022年第5期36卷 734-742页

作者： ZHANG xing-yu LYU Xu-jian fan Xu-dong School of Energy and Power Engineering Nanjing University of Science and TechnologyNanjing 210094China

When a high-speed body with cavity passes through water-air free surface and exits water,its mechanical environment and dynamic characteristics change significantly due to the great difference in density and viscosity between water and *** focusing on this problem,the Computational Fluid Dynamics(CFD)method is applied to perform numerical calculation on the process of this vapor-liquid-gas flow during the water exit of a high-speed cylinder,with the Volume of Fraction(VOF)multiphase flow interface-capturing techniques and the overset grid *** the verification and validation of the CFD model through mesh convergence study and a water-entry experiment,cavity evolution and flow characteristics including pressure and velocity distribution during the water exit are *** effects of different initial velocities on the pressure distribution and drag characteristics of the cylinder are *** results show that the cavity collapse during water exit causes strong pressure fluctuation on the cylinder;when the cylinder exits water enveloped in a supercavity,the pressure distribution on its wall surface and surrounding water region is relatively uniform,and the drag changes gently,and thus the cylinder has good motion stability.

关键词： water exit cavity CFD

Comparative transcriptomic analysis of larval and adult Malpighian tubules from the cotton bollworm Helicoverpa armigera

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Insect Science 2018年第6期25卷 991-1005页

作者： Yi-Yang Yuan Mei Li fan fan xing-Hui Qiu State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijingChina College of Plant Protection Hebei Agricultural UniversityBaodingHebei ProvinceChina

Malpighian tubules (MTs)are moregulatory organs of ***, usually considered the key excretory and os- increasing evidence has suggested that MTs perform many more functions than just *** now,the molecular and physiological functions of MTs in the cotton bollworm (Helicoverpa armigera),a very important agricultural pest,are largely *** this study,the transcriptomes of H. armigera MTs from larvae,male adults and female adults were sequenced using RNA-Seq technology,and comparative analyses of transcriptomes between two life stages (larval and adult)and between adult sexes were *** generated a total of 84 643 high-quality unigenes,and identified a large number of abundant transcripts putatively encoding proteins involved in diuresis,detoxification,immunity,carbohydrate transport and metabolism,development and *** found that the expression pattern of unigenes was relatively similar between female and male adult MTs,but different between larval and adult *** data suggest that insect MTs may take multiple physiological fimctions as versatile *** extensive alterations in gene expression in MTs occurred from larvae to adults reflect an ecological adaptation to different feeding *** dimorphism in the cotton bollworm is somewhat indicated by the transcriptional difference of genes related to carbohydrate metabolism,detoxification,immunity and reproduction in the MTs of male and female adults.

关键词： ecological adaptation Helicoverpa armigera Malpighian tubules sexual dimorphism transcriptome

Seismic data reconstruction based on low dimensional manifold model

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Petroleum Science 2022年第2期19卷 518-533页

作者： Nan-Ying Lan fan-Chang Zhang xing-Yao Yin School of Geosciences China University of Petroleum(East China)QingdaoShandong 266580China

Seismic data reconstruction is an essential and yet fundamental step in seismic data processing workflow,which is of profound significance to improve migration imaging quality,multiple suppression effect,and seismic inversion *** methods play a central role in solving the underdetermined inverse problem of seismic data *** this paper,a novel regularization approach is proposed,the low dimensional manifold model(LDMM),for reconstructing the missing seismic *** work relies on the fact that seismic patches always occupy a low dimensional ***,we exploit the dimension of the seismic patches manifold as a regularization term in the reconstruction problem,and reconstruct the missing seismic data by enforcing low dimensionality on this *** crucial procedure of the proposed method is to solve the dimension of the patches *** this,we adopt an efficient dimensionality calculation method based on low-rank approximation,which provides a reliable safeguard to enforce the constraints in the reconstruction *** experiments performed on synthetic and field seismic data demonstrate that,compared with the curvelet-based sparsity-promoting L1-norm minimization method and the multichannel singular spectrum analysis method,the proposed method obtains state-of-the-art reconstruction results.

关键词： Seismic data reconstruction Low dimensional manifold model Regularization Low-rank approximation

A new semi-empirical model for soil moisture content retrieval by ASAR and TM data in vegetation-covered areas

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Science China Earth Sciences 2011年第12期54卷 1955-1964页

作者： YU fan zhao YingShi Chinese Academy of Surveying and Mapping Beijing 100830 China Graduate University of Chinese Academy of Sciences Beijing 100049 China

Active microwave and passive optical remote sensing data have demonstrated their respective advantages in inversion of surface soil moisture content. A new semi-empirical model is presented for soil moisture content retrieval in vegetation-covered areas, using ENVISAT-ASAR and LANDSAT-TM data collaboratively. Derivation of the algorithm is based on simplification of the Michigan Microwave Canopy Scattering Model （MIMICS）. In the model, the ground surface is divided into a canopy layer and a soil layer, and empirical relationships simulated among vegetation water mass We, the backscatter coefficient σpq1, the bidirectional scattering coefficient σpq2 and the extinction coefficient τp. The key input parameters of the semi-empirical model are reduced to only the leaf area index （LAI）, which can be easily inverted by the optical model PROSAIL, allowing coupling of the microwave and optical models to be achieved. Also, vegetation RMS height （Svcg） is introduced to correct for the radar-shadow effect caused by over-laying vegetation. Analysis of the parameter sensitivity of the semi-empirical model showed that when the regional Leaf Area Index is small （LAI≤3）, the model is more applicable. Soil moisture distribution in the study area was mapped using the semi-empirical model and field ground measurements used for model validation. This showed that, after correction of the radar-shadow effect, the average relative error （Er） between ground-measured and semi-empirical model-derived estimates of soil moisture decreased from 17.6% to 10.4%, while the RMS reduced from 0.055 to 0.031 g cm^-3. The accuracy of soil moisture estimates from the semi-empirical model is much better than for the MIMICS model （Er = 22.7%, RMS = 0.068 g cm^-3）, showing that the semi-empirical model is efficient at obtaining regional surface soil moisture contents when LAI is small.

关键词： microwave and optic remote sensing MIMICS PROSAIL soil moisture

Subspace choices for the Celis-Dennis-Tapia problem

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Science China Mathematics 2017年第9期60卷 1717-1732页

作者： zhao Xin fan JinYan School of Mathematical Sciences Shanghai Jiao Tong UniversityShanghai 200240China School of Mathematical Sciences and MOE-LSC Shanghai Jiao Tong UniversityShanghai 200240China

Grapiglia et al.(2013) proved subspace properties for the Celis-Dennis-Tapia(CDT) problem. If a subspace with lower dimension is appropriately chosen to satisfy subspace properties, then one can solve the CDT problem in that subspace so that the computational cost can be reduced. We show how to find subspaces that satisfy subspace properties for the CDT problem, by using the eigendecomposition of the Hessian matrix of the objection function. The dimensions of the subspaces are investigated. We also apply the subspace technologies to the trust region subproblem and the quadratic optimization with two quadratic constraints.

关键词： CDT problems subspace properties subspace choices

Fabrication of Ordered Magnetite-Doped Rare Earth Fluoride Nanotube Arrays by Nanocrystal Self-Assembly

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Nano Research 2009年第4期2卷 292-305页

作者： fan Zhang Dongyuan zhao Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials Department of Chemistryand Laboratory of Advanced MaterialsFudan UniversityShanghai 200433China

We describe a nanocrystal self-assembly method for the preparation of rare earth fluoride nanotube(ReF-NT)arrays and magnetite-doped rare earth fluoride nanotubes(Fe_(3)O_(4)ReF-NTs)by using porous anodic aluminum oxide(AAO)as a hard *** ReF-NTs can be simply prepared by the impregnation of-NaYF_(4)nanocrystals doped with Yb and Er into the channels of the porous AAO and show a highly ordered nanotube array and excellent upconversion(UC)fluorescence ***,the Fe_(3)O_(4)ReF-NTs are obtained by the self-assembly of a mixture of Fe_(3)O_(4)and Yb/Er doped-NaYF4 nanocrystals in the AAO pore channels and have a uniform dispersion of magnetite nanocrystals on the rare earth fluoride tube matrix and possess multifunctional magnetic/UC *** diameter of these nanotubes can be varied from 60 nm to several micrometers depending on the pore size of the AAO *** wall thickness can be increased from 10 to 35 nm by increasing the concentration of nanocrystals from 0.02 to 0.4 mmol/L,while the morphology of the nanotubes can be varied from small isolated domain structures to percolating domains and eventually to compact domains.A template-directed formation mechanism is proposed and the quantitative predictions of the model for such self-assembled nanocrystal spreading processes are *** UC fluorescent emissions are realized for the nanotube arrays and multifunctional nanotubes with UC excitation in the near-infrared(NIR)region.A strong magnetic response of the multifunctional nanotubes is observed,which facilitates their easy separation from solution by magnetic decantation using a permanent magnet.

关键词： Nanotubes synthesis nanocrystals self-assembly upconversion luminescence magnetic rare earth fluorides

Derivative estimates of averaging opera tors and extension

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Frontiers of Mathematics in China 2019年第2期14卷 475-491页

作者： Junyan zhao Dashan fan Department of Mathematics Zhejiang University Hangzhou 310027 China Department of Mathematical Sciences University of Wisconsin-Milwaukee Milwaukee WI 53201 USA

We study the derivative operator of the generalized spherical mean S^γt. By considering a more general multiplier m^Ωγ,b=Vn-2/2+γ(|ξ|)|ξ|^bΩ(ξ') and finding the smallest γ such that m^Ωγ,b is an Hp multiplier, we obtain the optimal range of exponents (γ,β,p)to ensure the H^p(R^n) boundedness of a^βS^γ1f(x). As an application, we obtain the derivative estimates for the solution for the Cauchy problem of the wave equation on H^p(R^n) spaces.

关键词： Generalized spherical mean Bessel function H^p multiplier wave equation oscillatory integrals