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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第8期9卷 656-661页

作者： Yi-feng JIANG Feng-di zhao Xiao-bo li yan-xia NING Xiu-ling ZHI Rui-zhe QIAN lian-hua YIN Department of Physiology and Pathophysiology Shanghai Medical College Fudan University Shanghai 200032 China

Objective： To explore the effects of down-regulated tryptase expression in mast cells on the synthesis and release of interleukin-6 （IL-6） and tumor necrosis factor-alpha （TNF-α） of vascular endothelial cells. Methods： Tryptase-siRNA （small-interfering RNA） vector was constructed to inhibit tryptase expression in P815 cells. The medium of P815 cells treated by the tryptase-siRNA （RNAi-P815 group） or pure vector （P815 group） was collected and used to culture bEnd.3 cells. The messenger RNAs （mRNAs） of IL-6 and TNF-α in bEnd.3 cells and their protein levels in the medium were measured by reverse transcription polymerase chain reaction （RT-PCR） and enzyme-linked immunosorbent assay （EliSA）, respectively. Results： IL-6 and TNF-α mRNAs in bEnd.3 cells cultured in RNAi-P815-conditioned medium decreased significantly compared to those in P815-conditioned medium. Consistently, lL-6 and TNF-α protein levels in the medium of bEnd.3 of RNAi-P815 group were lower than those of P815 group. Conclusion： Reduced tryptase expression significantly inhibited the synthesis and release of IL-6 and TNF-α in vascular endothelial cells. RNA interference targeting tryptase expression may be a new anti-inflammatory strategy for vascular diseases.

关键词： Tryptase RNA interference lnterleukin-6 (IL-6) Tumor necrosis factor-alpha (TNF-α) Endothelial cells

A Multiple Phase-Shifted Distributed Feedback(DFB)Laser Fabricated by Nanoimprint lithography

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Chinese Physics Letters 2013年第5期30卷 67-69页

作者： ZUO Qiang zhao Jian-Yi CHEN Xin WANG Zhi-Hao SUN Tang-You ZHOU Ning zhao yan-li XU Zhi-Mou liU Wen Wuhan National Laboratory for Optoelectronics School of Optical and Electronic InformationHuazhong University of Science and TechnologyWuhan 430074 Accelink Technologies Company LtdWuhan 430074

Multiple phase-shifted(MPS)diffraction grating is an effective way proposed to overcome the spatial hole burning(SHB)effect in a distributed feedback(DFB)*** present two symmetric λ/8 phase-shifted DFB lasers by using nanoimprint lithography(NIL).The threshold current of a typical laser is less than 15 *** side mode suppression ratio(SMSR)is still above 42 dB even at 100 mA current *** show the versatility of NIL,eight different wavelength MPS-DFB lasers on this single chip are also *** results prove that NIL is a promising tool for fabricating high performance complex grating DFB lasers.

关键词： lithography shifted overcome

Microstructure and Mechanical Property of Mg‑13Gd‑0.2Ni Alloy Processed by Extrusion and Aging

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Acta Metallurgica Sinica(English Letters) 2024年第8期37卷 1367-1376页

作者： Hang Zhang Jiaying zhao Rongguang li Boshu liu Shanshan li Sha Sha Yuehong Zhang Man Xu yan Tang School of Mechanical and Power Engineering Shenyang University of Chemical TechnologyShenyang 110142China Instrumental Analysis Center Shenyang University of Chemical TechnologyShenyang 110142China

In this work,a good combination of strength and ductility is achieved in a Mg-13Gd-0.2Ni alloy by conventional extrusion and following aging *** aged Mg-13Gd-0.2Ni alloy exhibits a yield strength(YS)of 363 MPa,an ultimate tensile strength(UTS)of 433 MPa,and an elongation of 11.9%.The aged Mg-13Gd-0.2Ni alloy contains a microstructure with a 95%proportion of dynamically recrystallized(DRXed)grains with a micron size,weak texture,and a high number density of prismaticβ′precipitates within *** bulk compounds enriched with Ni element which are mainly formed during casting and stable in the extruded and aged *** dynamic compounds are formed during extrusion,and a high density of prismaticβ′precipitates are formed during *** high density of prismaticβ′precipitates results in a significant increase in the strength of the Mg-13Gd-0.2Ni alloy,and the YS and UTS are increased by 153 and 136 MPa,*** high proportion of DRXed grains with a weak texture contributes mainly to the high ductility and the fine compounds with a low density at grain boundaries formed during aging have no significant adverse effect on ductility of the aged Mg-13Gd-0.2Ni *** findings for the novel Mg-Gd-Ni alloy can provide guidance for the design of wrought Mg alloys with superior mechanical properties.

关键词： Mg-Gd-Ni alloy Extrusion Aging Strength Ductility

An efficient and universal protoplast-based transient gene expression system for genome editing in Brassica crops

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Horticultural Plant Journal 2024年第4期10卷 983-994页

作者： Xiaoxiao Yu Jintai Yu Yin Lu Wenjing li Guanzhong Huo Jun Zhang yan li Jianjun zhao Jun li State Key Laboratory of North China Crop Improvement and Regulation College of Life SciencesHebei Agricultural UniversityBaodingHebei 071001China Hebei Key Laboratory of Plant Physiology and Molecular Pathology Hebei Agricultural UniversityBaodingHebei 071001China Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei Collaborative Innovation Center of Vegetable Industry in HebeiCollege of HorticultureHebei Agricultural UniversityBaodingHebei 071001China College of Modern Science and Technology Hebei Agricultural UniversityBaodingHebei 071001China Human Resource Department Hebei Agricultural UniversityBaodingHebei 071001China

Protoplast-based transient gene expression system has been widely used in plant genome editing because of its simple operation and less *** order to establish a universal protoplast-based transient transfection system for verifying activities of genome editing vectors containing targets in Brassica,we systematically optimized factors affecting protoplast isolation and transient gene *** established an efficient protoplast-based transient gene expression system(PTGE)in Chinese cabbage,achieving high protoplast yield of 4.9×10^(5)·g^(-1)FW,viability over 95%,and transfection efficiency of 76%.We showed for the first time that pretreatment of protoplasts with a hypotonic MMG could significantly enhance the transfection ***,protoplasts incubated at 37℃ for 6 min improved the transfection efficiency to 86%.We also demonstrated that PTGE worked well(more than 50%transfection efficiency)in multiple Brassica species including cabbage,Pak Choi,Chinese kale,and ***,PTGE was used for validating the activities of CRISPR/Cas9 vectors containing targets in Chinese cabbage,cabbage,and pak choi,demonstrating the broad applicability of the established PTGE for genome editing in Brassica crops.

关键词： Chinese cabbage PTGE Transfection efficiency CRISPR/Cas9

Genome-wide association study of grain micronutrient concentrations in bread wheat

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Journal of Integrative Agriculture 2024年第5期23卷 1468-1480页

作者： Yongchao Hao Fanmei Kong lili Wang Yu zhao Mengyao li Naixiu Che Shuang li Min Wang Ming Hao Xiaocun Zhang yan zhao State Key Laboratory of Crop Biology/College of Agronomy Shandong Agricultural UniversityTai'an 271018China College of Resources and Environment Shandong Agricultural UniversityTai'an 271018China Jining liangshan Ecological Environment Affairs Center Jining 272100China College of Forestry Shandong Agricultural UniversityTai'an 271018China

Bread wheat(Triticum aestivum)is a staple food crop *** genetic dissection of important nutrient traits is essential for the biofortification of wheat to meet the nutritional needs of the world's growing ***,45,298 single-nucleotide polymorphisms(SNPs)from 55K chip arrays were used to genotype a panel of 768 wheat cultivars,and a total of 154 quantitative trait loci(QTLs)were detected for eight traits under three environments by genome-wide association study(GWAS).Three QTLs(qMn-3B.1,qFe-3B.4,and qSe-3B.1/qFe-3B.6)detected repeatedly under different environments or traits were subjected to subsequent analyses based on linkage disequilibrium decay and the P-values of significant *** SNPs in the three QTL regions formed six haplotypes for qMn-3B.1,three haplotypes for qFe-3B.4,and three haplotypes for qSe-3B.1/*** analysis revealed significant differences among *** results indicated that the concentrations of several nutrient elements have been modified during the domestication of landraces to modern *** on the QTL regions,we identified 15 high-confidence genes,eight of which were stably expressed in different tissues and/or developmental ***3B02G046100 in qMn-3B.1 and TraesCS3B02G199500 in qSe-3B.1/qFe-3B.6 were both inferred to interact with metal ions according to the Gene Ontology(GO)***3B02G199000,which belongs to qSe-3B.1/qFe-3B.6,was determined to be a member of the WRKY gene ***,this study provides several reliable QTLs that may significantly affect the concentrations of nutrient elements in wheat grain,and this information will facilitate the breeding of wheat cultivars with improved grain properties.

关键词： bread wheat nutritional element GWAS domestication haplotype

Inhalable metal–organic framework-mediated cuproptosis combined with PD-L1 checkpoint blockade for lung metastasis synergistic immunotherapy

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Acta Pharmaceutica Sinica B 2024年第5期14卷 2281-2297页

作者： Chongzheng yan Ying liu Guozhi zhao Huatian yang Huaiyou Lv Genju li Yuhan li Yaqing Fu Fengqin Sun Yafei Feng Yizhe li Zhongxi zhao Department of Pharmaceutics Key Laboratory of Chemical Biology of Ministry of EducationSchool of Pharmaceutical SciencesCheelloo College of MedicineShandong UniversityJinan 250012China Key University Laboratory of Pharmaceutics&Drug Delivery Systems of Shandong Province School of Pharmaceutical SciencesCheeloo College of MedicineShandong UniversityJinan 250012China

Cuproptosis shows enormous application prospects in lung metastasis ***,the glycolysis,Cu^(+)efflux mechanisms,and insufficient lung drug accumulation severely restrict cuproptosis ***,an inhalable poly(2-(N-oxide-N,N-diethylamino)ethyl methacrylate)(OPDEA)-coated copper-based metal–organic framework encapsulating pyruvate dehydrogenase kinase 1 siRNA(siPDK)is constructed for mediating cuproptosis and subsequently promoting lung metastasis immunotherapy,namely *** inhalation,OMP shows highly efficient lung accumulation and long-term retention,ascribing to the OPDEA-mediated pulmonary mucosa *** tumor cells,OMP is degraded to release Cu2+under acidic condition,which will be reduced to toxic Cu^(+)to induce cuproptosis under glutathione(GSH)***,siPDK released from OMP inhibits intracellular glycolysis and adenosine-5ʹ-triphosphate(ATP)production,then blocking the Cu^(+)efflux protein ATP7B,thereby rendering tumor cells more sensitive to OMP-mediated ***,OMP-mediated cuproptosis triggers immunogenic cell death(ICD)to promote dendritic cells(DCs)maturation and CD8^(+)T cells ***,OMP-induced cuproptosis up-regulates membrane-associated programmed cell death-ligand 1(PD-L1)expression and induces soluble PD-L1 secretion,and thus synergizes with anti-PD-L1 antibodies(aPD-L1)to reprogram immunosuppressive tumor microenvironment,finally yielding improved immunotherapy ***,OMP may serve as an efficient inhalable nanoplatform and afford preferable efficacy against lung metastasis through inducing cuproptosis and combining with aPD-L1.

关键词： Cuproptosis Immunogenic cell death PD-L1 checkpoint blockade Copper-based metal-organic framework Immunotherapy Tumor microenvironment Transmucosal deliveryLung metastasis

TOEPliTZ OPERATORS BETWEEN WEIGHTED BERGMAN SPACES OVER THE HALF-PLANE

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Acta Mathematica Scientia 2024年第5期44卷 1707-1720页

作者： lixia FENG yan li Zhiyu WANG liankuo zhao School of Mathematics and Computer Science Shanxi Normal UniversityTaiyuan030031China

In this paper,by characterizing Carleson measures,we investigate a class of bounded Toeplitz operator between weighted Bergman spaces with Békolléweights over the half-plane for all index choices.

关键词： weighted Bergman spaces Békolléweights Carleson measure Toeplitz operators

Control of defensive behavior by the nucleus of Darkschewitsch GABAergic neurons

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National Science Review 2024年第4期11卷 70-83页

作者： Huiying zhao Jinrong liu Yujin Shao Xiang Feng Binhan zhao li Sun Yijun liu linghui Zeng Xiao-ming li Hongbin yang Shumin Duan yan-qin Yu Department of Neurology of Second Affiliated Hospital and School of Brain Science and Brain Medicine Zhejiang University School of Medicine Nanhu Brain-Computer Interface Institute liangzhu Laboratory MOE Frontier Science Center for Brain Science and Brain-Machine Integration State Key Laboratory of Brain-Machine Intelligence Zhejiang University NHC and CAMS Key Laboratory of Medical Neurobiology Zhejiang University Key Laboratory of Novel Targets and Drug Study for Neural Repair of Zhejiang Province School of Medicine Hangzhou City University

The nucleus of Darkschewitsch(ND), mainly composed of GABAergic neurons, is widely recognized as a component of the eye-movement controlling system. However, the functional contribution of ND GABAergic neurons(NDGABA) in animal behavior is largely unknown. Here, we show that NDGABA neurons were selectively activated by different types of fear stimuli, such as predator odor and foot *** and chemogenetic manipulations revealed that NDGABA neurons mediate freezing ***, using circuit-based optogenetic and neuroanatomical tracing methods, we identified an excitatory pathway from the lateral periaqueductal gray(l PAG) to the ND that induces freezing by exciting ND inhibitory outputs to the motor-related gigantocellular reticular nucleus, ventral part(Gi V). Together,these findings indicate the NDGABA population as a novel hub for controlling defensive response by relaying fearful information from the l PAG to Gi V, a mechanism critical for understanding how the freezing behavior is encoded in the mammalian brain.

关键词： nucleus of Darkschewitsch GABAergic population defensive behavior freezing neural circuitry

Proactive Resilience Enhancement of Power Systems with link Transmission Model-based Dynamic Traffic Assignment Among Electric Vehicles

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CSEE Journal of Power and Energy Systems 2024年第3期10卷 1320-1330页

作者： Haoyuan yan Tianyang zhao Zhanglei Guan Energy Electricity Research Center Jinan UniversityGuangzhou 510632China Energy Research Institute Nanyang Technological UniversitySingapore IEEE

The rapid development of electric vehicles(EVs)is strengthening the bi-directional interactions between electric power networks(EPNs)and transportation networks(TNs)while providing opportunities to enhance the resilience of power systems towards extreme *** quantify the temporal and spatial flexibility of EVs for charging and discharging,a novel dynamic traffic assignment(DTA)problem is *** DTA problem is based on a link transmission model(LTM)with extended charging links,depicting the interaction between EVs and power *** models the charging rates as continuous variables by an energy boundary *** consider the evacuation requirements of TNs and the uncertainties of traffic conditions,the DTA problem is extended to a two-stage distributionally robust *** is further incorporated into a two-stage distributionally robust unit commitment problem to balance the enhancement of EPNs and the performance of *** problem is reformulated into a mixed-integer linear programming problem and solved by off-the-shelf commercial *** studies are performed on two test *** effectiveness is verified by the numerical results,e.g.,reducing the load shedding amount without increasing the unmet traffic demand.

关键词： Coupled transportation and power network link transmission model resilience enhancement

Application of ultrasonic fatigue technology in very-high-cycle fatigue testing of aviation gas turbine engine blade materials:A review

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Science China(Technological Sciences) 2024年第5期67卷 1317-1363页

作者： zhao JiuCheng WAN Jie ZHANG ShiZhong yan Chuliang zhao HongWei Key Laboratory of CNC Equipment Reliability Ministry of Education Jilin UniversityChangchun 130025China School of Mechanical&Aerospace Engineering Jilin UniversityChangchun 130025China Chongqing Research Institute of Jilin University Chongqing401120China School of Aeronautic Science and Engineering Beihang UniversityBeijing 102206China

The need for very-high-cycle fatigue(VHCF)testing up to 1010cycles of aviation gas turbine engine blade materials under combined mechanical loads and complex environments has encouraged the development of VHCF testing instrumentation and *** article begins with a comprehensive review of the existing available techniques that enable VHCF *** advances in ultrasonic fatigue testing(UFT)techniques are highlighted,containing their new capabilities and methods for single load,multiaxial load,variable amplitude fatigue,and combined cycle *** techniques for conducting UFT in high-temperature,humid environments,and corrosive environments are *** developments in mechanical loading and environmental building techniques provide the possibility of laboratory construction for real service conditions of blade *** techniques that can be used for in situ monitoring of VHCF damage are *** issues in the UFT field are presented,and countermeasures are ***,the existing problems and future trends in the field are briefly described.

关键词： aviation gas turbine engine blade materials ultrasonic fatigue very-high-cycle fatigue high-temperature complex stress in situ testing