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A Transcriptional Network Promotes Anthocyanin Biosynthesis in Tomato Flesh

Molecular Plant 2020年第1期13卷 42-58页

作者： Chuanlong Sun Lei Deng Minmin Du Jiuhai zhao qian Chen Tingting huang Hongling Jiang chang-Bao Li Chuanyou Li State Key Laboratory of Plant Genomics National Centre for Plant Gene Research(Beijing)Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China) Ministry of AgricultureBeijing Vegetable Research CenterBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China State Key Laboratory of Crop Biology College of AgronomyShandong Agricultural UniversityTai'anShandong Province 271018China lnstitute of Vegetable Qingdao Academy of Agricultural SciencesQingdaoShandong Province 266100China

Dietary anthocyanins are important health-promoting antioxidants that make a major contribution to the quality of fruits. It is intriguing that most tomato cultivars do not produce anthocyanins in fruit. However, the purple tomato variety Indigo Rose, which has the dominant Aft locus combined with the recessive atv locus from wild tomato species, exhibits light-dependent anthocyanin accumulation in the fruit skin. Here, we report that Aft encodes a functional anthocyanin activator named SlAN2-like, while atv encodes a nonfunctional version of the anthocyanin repressor SlMYBATV. The expression of SlAN2-like is responsive to light, and the functional SlAN2-like can activate the expression of both anthocyanin biosynthetic genes and their regulatory genes, suggesting that SlAN2-like acts as a master regulator in the activation of anthocyanin biosynthesis. We further showed that cultivated tomatoes contain nonfunctional alleles of SlAN2-like and therefore fail to produce anthocyanins. Consistently, expression of a functional SlAN2-like gene driven by the fruit-specific promoter in a tomato cultivar led to the activation of the entire anthocyanin biosynthesis pathway and high-level accumulation of anthocyanins in both the peel and flesh. Taken together, our study exemplifies that efficient engineering of complex metabolic pathways could be achieved through tissue-specific expression of master transcriptional regulators.

关键词： whole-genome duplication paleopolyploidy adaptive evolution phylogenomic Cretaceous-Paleocene boundary gene regulatory network

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The biomethane producing potential in China： A theoretical and practical estimation

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Chinese Journal of Chemical Engineering 2016年第7期24卷 920-928页

作者： chang Liu Jun Wang Xiaoyan Ji Hongliang qian Liangliang huang Xiaohua Lu College of Chemistry and Chemical Engineering Nanjing Tech University Nanjing210009 China School of Chemical Biological & Materials Engineering University of Oklahoma Norman 73019 United States Division of Energy Science Lulea University of Technology 97187 Lulea Sweden School of Pharmacy China Pharmaceutical University Nanjing 210009 China

Biomethane has been developed rapidly in many countries as a renewable energy which upgraded from biogas. China also began to pay attention to it even though we still at a initial stage, primarily, understanding the hiomethane potential and development prospect, choosing appropriate biomass as the biomethane source is very important. In this work, the theoretical and practical biomethane producing potential from five main bio- mass resources in China were estimated with appropriate methods based on the data collected, and during cal- culation, two appropriate energy crops were assumed to be planted on marginal lands for biomethane production. Our estimation showed that the theoretical and practical biomethane potentials in China can reach to 888.78 and 316.30 billion m3 per year, agricultural waste should be the preferential development biomass, and planting energy crops on marginal lands is the most promising way to enhance biomethane production in China. Finally, biomethane is compared with natural gas, and the result showed that 48.15% of the practical biomethane potential can meet the total Chinese natural gas consumption in 2013.

关键词： Biomethane potentialBiomassMarginal landEnergy crop

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Microstructure,texture and precipitates of grain-oriented silicon steel produced by thin slab casting and rolling process

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Journal of Iron and Steel Research International 2017年第1期24卷 91-96页

作者： Si-qian Bao Yang Xu Gang zhao Xiang-bin huang Huan Xiao Chuan-long Ye Na-na Song Qing-ming chang The State Key Laboratory of Refractories and Metallurgy Wuhan University of Science and TechnologyWuhan 430081HubeiChina Key Laboratory for Ferrous Metallurgy and Resources Utilization of Ministry of Education Wuhan University of Science and TechnologyWuhan 430081HubeiChina

A grain-oriented silicon steel strip with AlN as main inhibitor was produced by thin slab casting and rolling（TSCR）*** microstructure,texture and precipitates of the hot-rolled strip were investigated by use of optical microscope（OM）,X-ray diffractometer,transmission electron microscope（TEM）and energy dispersive spectroscope（EDS）.The result shows that the microstructure and texture exhibit a through-thickness gradient similar to that of the hot-rolled strip produced by conventional high-temperature slab-reheating process;the preferred orientation varies from {110}〈001〉in the surface layer to{001}〈110〉in the center layer,and the Goss texture with a maximum intensity mainly concentrates on the surface *** addition,some other texture components,for example rotated Goss texture,form in the 1/4thickness layer,which are not observed in the hotrolled strip produced by conventional high-temperature slab-reheating *** precipitates in the hot-rolled strip are mainly（Mn,Cu）S and AlN compound particles with dimension of 100-200 nm,and the fine precipitates are significantly less than that in the hot-rolled strip produced by conventional high-temperature slab-reheating ***,the areal density of the fine precipitates in the center layer is more than that in the surface layer.

关键词： Grain-oriented silicon steel Thin slab casting and rolling process Microstructure Texture Precipitate

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Intranasal endoscopic prelacrimal recess approach to maxillary sinus

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Chinese Medical Journal 2013年第7期126卷 1276-1280页

作者： ZHOU Bing HAN De-min CUI shun-jiu huang qian WANG Cheng-shuo Department of Otolaryngology-Head and Neck Surgery Beijing Tongren Hospital Capital Medical University Key Laboratory of Otolaryngology Head and Neck Surgery (Capital Medical University) Ministry of Education Beijing 100730 China

Background The inferior turbinate （IT） and nasolacrimal duct （NLD） are often sacrificed while managing the diffuse lesion of maxillary sinus （MS）. We report a new approach to MS without ablation of NLD and IT. Methods This retrospective study enrolled 19 hospitalized patients （aged from 42 to 68 years） who underwent endoscopic sinus surgery between 2003 and 2008. Twelve patients had inverted papilloma （IP）, two had nasal polyps, two had Kubo＇s postoperative cyst of MS, one had recurrent bone cyst of maxilla, one had dentigerous cyst and one had bleeding of internal maxillary artery secondary to CaldwelI-Luc operation respectively. Two IP patients were excluded from this group since the follow-up time was less than 12 months. The NLD was dissected after removing the anterior bony portion of nasal lateral wall. The prelacrimal recess approach （PLRA） to MS was established when IT-NLD flap was raised medially. The flap was repositioned when MS lesion was removed. Results All the 17 patients had unilateral lesions. Ten MS IP patients were at the T3 Krouse stage. The follow-up ranged from 7 to 60 months. No recurrence was seen in 16 patients. Only one IP patient had a local recurrence in MS. All of them had no any complications. Conclusion The diffuse or severe diseases of MS may be the potential indications for PLRA.

关键词： nasal endoscope maxillary sinus prelacrimal recess inferior turbinate nasolacrimal duct

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Characterization of subunits encoded by Sn RK1 and dissection of combinations among these subunits in sorghum(Sorghum bicolor L.)

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Journal of Integrative Agriculture 2023年第2期22卷 642-649页

作者： XIAO qian-lin huang Tian-hui ZHOU chang CHEN Wei-xi CHA Jian-kui WEI Xi-mei XING Fang-yu qian Meng-ya MA qian-nan DUAN Hong LIU Zhi-zhai College of Agronomy and Biotechnology Southwest UniversityChongqing 400715P.R.China

Sucrose nonfermenting-related protein kinase 1(SnRK1)is one of the critical serine/threonine protein *** commonly mediates plant growth and development,cross-talks with metabolism processes and physiological responses to biotic or abiotic *** plays a key role in distributing carbohydrates and sugar signal *** the present study,eight SnRK1 coding genes were identified in sorghum(Sorghum bicolor L.)via sequences alignment,with three forαsubunits(SnRK1α1 to SnRK1α3),three forβ(SnRK1β1 to SnRK1β3),and one for bothγ(SnRK1γ)andβγ(SnRK1βγ).These eight corresponding genes located on five chromosomes(Chr)of Chr1–3,Chr7,and Chr9 and presented collinearities to SnRK1s from maize and rice,exhibiting highly conserved domains within the same subunits from the three kinds of *** results via qRT-PCR showed that different coding genes of SnRK1s in sorghum possessed similar expression patterns except for SnRK1α3 with a low expression level in grains and SnRK1β2 with a relatively high expression level in *** of subcellular localization in sorghum leaf protoplast showed that SnRK1α1/α2/α3/γmainly located on organelles,while the rest four of SnRK1β1/β2/β3/βγlocated on both membranes and some ***,three combinations were discovered among eight SnRK1 subunits in sorghum through yeast two hybrid,includingα1-β2-βγ,α2-β3-γ,andα3-β3-γ.These results provide informative references for the following functional dissection of SnRK1 subunits in sorghum.

关键词： sorghum(Sorghum bicolor L.) SnRK1 expression analysis combination pattern

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重庆市绿色保险推动产业绿色发展研究——基于绿色金融改革创新试验区的实证分析

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当代金融研究 2024年第6期 88-104页

作者：曾青峰史倩琳黄昌兵三峡人寿保险股份有限公司重庆401121 西南大学经济管理学院重庆400715

我国提出“双碳”目标具有重要意义,推行绿色保险不仅有助于优化经济发展模式,更体现了我国作为全球领导者所肩负的责任与大国的担当。梳理分析绿色保险的国际国内经验和重庆绿色保险的发展现状,并基于2015-2021年重庆、衢州、湖州和广... 详细信息

我国提出“双碳”目标具有重要意义,推行绿色保险不仅有助于优化经济发展模式,更体现了我国作为全球领导者所肩负的责任与大国的担当。梳理分析绿色保险的国际国内经验和重庆绿色保险的发展现状,并基于2015-2021年重庆、衢州、湖州和广州4个绿色金融改革创新试验区的面板数据,研究绿色保险对产业绿色发展的影响。实证结果发现:绿色保险在推动产业绿色发展方面发挥了积极作用,绿色金融改革创新试验区的建立有效推动了绿色保险与产业绿色发展的耦合度及耦合协调度的提升并且重庆绿色保险对产业绿色发展的推动作用接近绿色金融改革创新试验区的整体水平。为了进一步推动重庆产业绿色发展,建议通过加大政策法律保障,完善绿色评价体系,创新产品与服务,加强绿色投资,加大金融科技运用,加快数字化新基建,广泛交流与研讨,大力发展绿色保险。

关键词：绿色保险产业绿色发展试验区

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HL-2A 装置边缘湍流与带状流相互作用的实验研究

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核聚变与等离子体物理 2024年第1期44卷 72-77页

作者：方茜程钧王威策严龙文黄治辉吴娜许宇鸿唐昌建石中兵钟武律许敏西南交通大学物理科学与技术学院成都610031 核工业西南物理研究院成都610041

利用组合静电探针阵列研究了HL-2A装置中湍流与带状流相互作用的动力学过程。实验结果表明,在粒子注入后的等离子体恢复阶段,边界区径向电场剖面逐渐变陡,湍流相干尺度和带状流幅度相应增加,而湍流强度随之降低。这些实验提供了带状流... 详细信息

利用组合静电探针阵列研究了HL-2A装置中湍流与带状流相互作用的动力学过程。实验结果表明,在粒子注入后的等离子体恢复阶段,边界区径向电场剖面逐渐变陡,湍流相干尺度和带状流幅度相应增加,而湍流强度随之降低。这些实验提供了带状流与边缘湍流相互作用的直接实验证据,暗示了能量转移是引起带状流增长的主要原因。

关键词：托卡马克带状流湍流输运静电探针双谱分析

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经鼻高流量氧疗对急性呼吸衰竭患者呼吸功能及预后的影响

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四川解剖学杂志 2024年第1期32卷 47-49,56页

作者：李杨李凤芝黄晗常瑞胡朝阳刘倩倩张小红郑州大学附属郑州中心医院呼吸与危重症医学科病区郑州450000

目的:探讨经鼻高流量氧疗对急性呼吸衰竭(ARF)患者呼吸功能及预后的影响.方法:选取2021年11月至2022年11月于本院收治的90例ARF患者作为研究对象.按照数字表法随机将其分为高流组(n=45,采取经鼻高流量氧疗)和普通组(n=45,采取经鼻导管氧... 详细信息

目的:探讨经鼻高流量氧疗对急性呼吸衰竭(ARF)患者呼吸功能及预后的影响.方法:选取2021年11月至2022年11月于本院收治的90例ARF患者作为研究对象.按照数字表法随机将其分为高流组(n=45,采取经鼻高流量氧疗)和普通组(n=45,采取经鼻导管氧疗).对两组动脉血气分析指标和呼吸功能,疾病转归和不良事件发生率进行统计学分析.结果:治疗后,两组患者动脉血氧分压(PaO_(2))、血氧饱和度(SpO_(2))均较治疗前上升,且高流组上升程度较普通组均更明显;动脉二氧化碳分压(PaCO_(2))、心率、呼吸频率(RR)、劳累度评估量表(Borg)评分和舒适度评分均较治疗前下降,且高流组下降程度较普通组均更明显,差异均有统计学意义(P<0.05).高流组ICU停留和住院时间、机械通气使用、院内感染、肺不张及不良事件总发生率均低于普通组,差异均有统计学意义(P<0.05).结论:经鼻高流量氧疗能有效改善ARF患者呼吸功能及预后情况.

关键词：经鼻高流量氧疗急性呼吸衰竭呼吸功能预后

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揿针联合有氧运动治疗老年单纯性肥胖病的临床疗效分析

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中国疗养医学 2024年第4期33卷 86-90页

作者：牛丹妹黄文倩杨常青河南省漯河市医学高等专科学校第三附属医院河南漯河462000

目的探讨揿针联合有氧运动对老年单纯性肥胖病的治疗效果。方法 2021年3月至2022年12月,于河南省漯河市医学高等专科学校第三附属医院选取115例老年单纯性肥胖病患者作为研究对象,随机数字表分为观察组(57例)和对照组(58例)。对照组采... 详细信息

目的探讨揿针联合有氧运动对老年单纯性肥胖病的治疗效果。方法 2021年3月至2022年12月,于河南省漯河市医学高等专科学校第三附属医院选取115例老年单纯性肥胖病患者作为研究对象,随机数字表分为观察组(57例)和对照组(58例)。对照组采用有氧运动治疗,观察组在对照组基础上联合揿针治疗,并对所有患者进行至少6个月跟踪随访。治疗前后及末次随访时,对所有患者进行身体指标测量,并检测血清血管内皮生长因子C(VEGF-C)、delta样配体4(DLL4)和肾上腺髓质素(ADM)等指标变化。结果治疗后,两组体质量指数(BMI)比较,差异无统计学意义(P>0.05),但观察组腰臀比(WHR)、体脂肪率(FAT)和内脏脂肪面积(VFA)均小于对照组(P<0.05)。治疗8、12周以及末次随访时,观察组血清VEGF-C、DLL4和ADM均低于对照组(P<0.05)。治疗8周、12周以及末次随访时,观察组外周血低密度脂蛋白胆固醇(LDL-C)低于对照组(P<0.05),高密度脂蛋白胆固醇(HDL-C)高于对照组(P<0.05)。治疗12周,观察组总胆固醇(TC)低于对照组(P<0.05)。结论揿针联合有氧运动治疗老年单纯性肥胖病可显著改善患者的身体指标。

关键词：老年单纯性肥胖病揿针有氧运动肠淋巴管功能相关因子安全性

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Characteristics of indoor/outdoor PM_(2.5) and elemental components in generic urban, roadside and industrial plant areas of Guangzhou City, China

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Journal of Environmental Sciences 2007年第1期19卷 35-43页

作者： huang Hong LEE shun-cheng CAO Jun-ji ZOU chang-wei CHEN Xin-geng FAN Shao-jia School of Chemistry and Environment South China Normal University Guangzhou 510006 China Department of Civil and Structural Engineering Hong Kong Polytechnic University Hang Hum Kowloon Hong Kong Institute of Earth Environment Chinese Academy of Sciences Xi＇an 710075 China School of Environmental Science and Engineering Nanchang University Nanchang 330029 China School of Environmental Science and Engineering SunYatSen University Guangzhou 510275 China

Quantitative information on mass concentrations and other characteristics, such as spatial distribution, seasonal variation, indoor/outdoor （I/O） ratio, correlations and sources, of indoor and outdoor PM2.5 and elemental components in Guangzhou City were provided. Mass concentration of PM2.5 and elemental components were determined by standard weight method and proton-induced X-ray emission （PIXE） method. 18 elements were detected, the results showed positive results. Average indoor and outdoor PM2.5 concentrations in nine sites were in the range of 67.7-74.5μg/m^3 for summer period, and 109.9-123.7 μg/m^3 for winter period, respectively. The sum of 18 elements average concentrations were 5362.6-5533.4 ng/m^3 for summer period, and 8416.8-8900.6 ng/m^3 for winter period, respectively. Average concentrations of PM2.5 and element components showed obvious spatial characteristic, that the concentrations in roadside area and in industrial plant area were higher than those in generic urban area. An obvious seasonal variation characteristic was found for PM2.5 and elemental components, that the concentrations in winter were higher than that in summer. The I/O ratio of PM2.5 and some elemental components presented larger than 1 sometimes. According to indoor/outdoor correlation of PM2.5 and element concentrations, it was found that there were often good relationships between indoor and outdoor concentrations. Enrichment factors were calculated to evaluate anthropogenic versus natural elements sources.

关键词： PM2.5 elemental components indoor air

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