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World Journal of Gastroenterology 2007年第37期13卷 5030-5034页

作者： Dong Hua zhao-hui huang Yong Mao Jian-Zhong Deng Oncology Institute of Wuxi the Fourth Affiliated Hospital ofSuzhou University Wuxi 214062 Jiangsu Province China

AIM： To investigate the prognostic role of thymidylate synthase （TS） and thymidine phosphorylase （TP） mRNA levels in T3 or T4 gastric cancer treated with 5-fluorouraci-based adjuvant chemotherapy. METHODS： Fifty-one patients with T3 or T4 gastric cancer received systemic 5-fluorouraci-based adjuvant chemotherapy, and intratumoral expression of TS and TP in 51 gastric cancer tissue samples was tested by realtime quantitative PCR.RESULTS： The median disease-free survival （DFS） time was 10.2 mo in the patients. There were no significant differences in DFS between the groups with high and low levels of TP. However, the group with low level of TS had a longer DFS （14.4 mo vs 8.3 mo, P = 0.017）. The median overall survival （OS） time was 18.5 mo, and there were significant differences in OS between the groups with high and low levels of TS or TP （for TS, 17.0 mo vs 21.3 mo, P = 0.010; for TP, 16.6 mo vs 22.5 too, P = 0.009）. Moreover, the coupled low expression of these two genes was strongly associated with a longer survival time of patients as compared with that of a single gene.CONCLUSION： Expression of TS and TP mRNA is a useful predictive parameter for the survival of postoperative gastric cancer patients after 5-fluorouracilbased adjuvant chemotherapy.

关键词： 5-fluorouracil Gastric cancer Thymidine phosphorylase Thymidylate synthase

Detection of aberrant methylation in fecal DNA as a molecular screening tool for colorectal cancer and precancerous lesions

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World Journal of Gastroenterology 2007年第6期13卷 950-954页

作者： zhao-hui huang Li-Hua Li Fan Yang Jin-Fu Wang Oncology Institute of Wu xi the Fourth Affiliated Hospital of Soochow University Wuxi 214062 Jiangsu Province China College of life sciences Zhejiang University Hangzhou 310012 Zhejiang Province China

AIM： To investigate the feasibility of detecting methylated fecal DNA as a screening tool for colorectal carcinoma （CRC） and precancerous lesions. METHODS： Methylated secreted frizzled-related protein gene 2 （SFRP2）, hyperplastic polyposis protein gene （HPP1） and O6-methylguanine-DNA methyltransferase gene （MGMT） in stools from 52 patients with CRC, 35 patients with benign colorectal diseases and 24 normal individuals were analyzed using methylation-specific PCR. RESULTS： Methylated SFRP2, HPP1 and MGMT were detected in 94.2%, 71.2%, 48.1% of CRC patients and 52.4%, 57.1%, 28.6% of adenoma patients, respectively. The overall prevalence of fecal DNA with at least one methylated gene was 96.2% and 81.8% in patients with CRC and precancerous lesions, respectively. In contrast, only one of the 24 normal individuals revealed methylated DNA. These results indicated a 93.7% sensitivity and a 77.1% specificity of the assay for detecting CRC and precancerous lesions. CONCLUSION： IVlethylation testing of fecal DNA using a panel of epigenetic markers may be a simple and promising non-invasive screening method for CRC and precancerous lesions.

关键词： Colorectal cancer Methylation Feces Secreted frizzled-related protein gene 2 Hyperplastic polyposis protein gene Methylguanine-DNA methyltransferase gene

GAUSS-SEIDEL-TYPE MULTIGRID METHODS

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Journal of Computational Mathematics 2003年第4期21卷 421-434页

作者： zhao-hui huang Qian-shun Chang (Academy of Mathematics and System Sciences, Chinese Academy of Sciences, Beijing 100080, China) Academy of Mathematics and System Sciences Chinese Academy of Sciences 北京 100080

By making use of the Gauss-Seidel-type solution method, the procedure for computing the interpolation operator of multigrid methods is simplified. This leads to a saving of computational time. Three new kinds of interpolation formulae are obtained by adopting different approximate methods, to try to enhance the accuracy of the interpolatory operator. A theoretical study proves the two-level convergence of these Gauss-Seidel-type MG methods. A series of numerical experiments is presented to evaluate the relative performance of the methods with respect to the convergence factor, CPU-time(for one V-cycle and the setup phase) and computational complexity.

关键词： Multigrid methods Gauss-Seidel solution Interpolation formula Convergence.

ERCC1 polymorphism, expression and clinical outcome of oxaliplatin-based adjuvant chemotherapy in gastric cancer

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World Journal of Gastroenterology 2008年第41期14卷 6401-6407页

作者： zhao-hui huang Dong Hua Xiang Du Li-Hua Li Yong Mao Zhi-hui Liu Ming-Xu Song Xi-Ke Zhou Institute the Fourth Affiliated Hospital of Suzhou University Wuxi 214062 Jiangsu Province China Department of Pathology Cancer Hospital Fudan University Shanghai 200032 China

AIM： TO determine the influence of excision repair cross complementing group 1 （ERCC1） codon 118 polymorphism and mRNA level on the clinical outcome of gastric cancer patients treated with oxaliplatin-based adjuvant chemotherapy. METHODS： Eighty-nine gastric cancer patients treated with oxalipatin-based adjuvant chemotherapy were included in this study. ERCC1 codon 118 C/T polymorphism was tested by polymerase chain reaction-ligation detection reaction （PCR-LDR） method in peripheral blood lymphocytes of those patients; and the intratumoral ERCC1 mRNA expression was measured using reverse transcription PCR in 62 patients whose tumor tissue specimens were available. RESULTS： No significant relationship was found between ERCC1 codon 118 polymorphism and ERCC1 mRNA level. The median relapse-free and overall survival period was 20.1 mo and 28.4 too, respectively. The relapse-free and overall survivals in patients with lOW levels of ERCC1 mRNA were significantly longer than those in patients with high levels （P 〈 0.05）, while there was no significant association found between ERCC1 118 genotypes and the disease prognosis. Multivariate analysis also showed that ERCC1 mRNA level was a potential predictor for relapse and survival in gastric cancer patients treated with oxaliplatin-based adjuvant chemotherapy （P 〈 0.05）. CONCLUSION： ERCC1 codon 118 polymorphisrn has no significant impact on ERCC1 rnRNA expression, and the intraturnoral ERCC1 rnRNA level but not codon 118 polymorphisrn may be a useful predictive parameter for the relapse and survival of gastric cancer patients receiving oxaliplatin-based adjuvant chemotherapy.

关键词： Gastric cancer Adjuvant chemotherapy Excision repair cross complementing group 1 Gene polymorphism

Microstructual evolution and stability of second generation single crystal nickel-based superalloy DD5

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Transactions of Nonferrous Metals Society of China 2016年第8期26卷 2079-2085页

作者： Ren-jie CUI zhao-hui huang Science and Technology on Advanced High Temperature Structural Materials Laboratory Beijing Institute of Aeronautical Materials Beijing 100095 China

The microstructual evolution and stability of a second generation single crystal (SC) nickel-based superalloy DD5 with minor grain boundary (GB) strengthening elements (C, B and Hf) were studied as a function of as-cast, heat treatment and thermal exposure. The microstructure and composition of the alloy were investigated by optical microscopy, scanning electron microanalysis (SEM), electron probe microanalysis (EPMA), energy dispersive spectrometry (EDS) and extraction analysis. In the as-cast condition,the microstructure observations and composition analysis showed that γ phase was the primary solidification phase and there were three microsegregations in the metal matrix. The morphology of these microsegregations depended on element segregations. After heat treatment, the dendrite cores contained fine and cuboidal-shaped γ′ particles with an average edge length of about 0.5 μm, whileinterdendritic regions contained irregularly-shaped γ′ particles and MC/M23C6 carbides. The mass fraction of γ′ phases was 61.685%.After exposure at 980 °C for 1000 h, no TCP phase was observed in both dendritic and interdendritic regions, indicating a good microstructual stability of the DD5 alloy at 980 °C.

关键词： single crystal superalloy DD5 alloy microstructural evolution heat treatment thermal exposure

Global research trends and prospects of cellular metabolism in colorectal cancer

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World Journal of Gastrointestinal Oncology 2024年第2期16卷 527-542页

作者： Yan-Chen Liu Zhi-Cheng Gong Chao-Qun Li Peng Teng Yan-Yan Chen zhao-hui huang Wuxi Cancer Institute Affiliated Hospital of Jiangnan UniversityWuxi 214122Jiangsu ProvinceChina Wuxi School of Medicine Jiangnan UniversityWuxi 214122Jiangsu ProvinceChina Wuxi Institute of Integrated Chinese and Western Medicine Affiliated Hospital of Jiangnan UniversityWuxi 214062Jiangsu ProvinceChina

BACKGROUND An increasing number of studies have focused on the role of cellular metabolism in the development of colorectal cancer(CRC).However,no work is currently available to synthesize the field through *** To analyze the development in the field of“glucose metabolism”(GM),“amino acid metabolism”(AM),“lipid metabolism”(LM),and“nucleotide metabolism”(NM)in CRC by *** Articles within the abovementioned areas of GM,AM,LM and NM in CRC,which were published from January 1,1991,to December 31,2022,are retrieved from the Web of Science Core Collection and analyzed by CiteSpace 6.2.R4 and VOSviewer *** The field of LM in CRC presented the largest number of annual publications and the fastest increase in the last decade compared with the other three ***,China and the United States were two of the most prominent contri-butors in these four *** addition,Gang Wang,Wei Jia,Maria Notar-nicola,and Cornelia Ulrich ranked first in publication numbers,while Jing-Yuan Fang,Senji Hirasawa,Wei Jia,and Charles Fuchs were the most cited authors on average in these four fields,respectively.“Gut microbiota”and“epithelial-mesenchymal transition”emerged as the newest burst words in GM,“gut microbiota”was the latest outburst word in AM,“metastasis”,“tumor microenvironment”,“fatty acid metabolism”,and“metabolic reprogramming”were the up-to-date outbreaking words in LM,while“epithelial-mesenchymal transition”and“apoptosis”were the most recently occurring words in *** Research in“cellular metabolism in CRC”is all the rage at the moment,and researchers are particularly interested in exploring the mechanism to explain the metabolic alterations in *** metabolic vulnerability appears to be a promising direction in CRC therapy.

关键词： Cellular metabolism Colorectal cancer Glucose metabolism Amino acid metabolism Lipid metabolism Nucleotide metabolism

Facile synthesis and characterization of novel thermo-chromism cholesteryl-containing hydrogen-bonded liquid crystals

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Chinese Chemical Letters 2009年第11期20卷 1303-1306页

作者： Wan Li He Tao Liu Zhou Yang Dong Yu zhao Wei huang hui Cao Guo Jie Wang Huai Yang Department of Materials Physics and Chemistry School of Materials Science and Engineering University of Science and Technology Beijing Beijing 100083 China

Two series of novel cholesteryl-containing H-bonded liquid crystals were prepared through facile self-assembly between cholesteryl isonicotinate （proton acceptor） exhibiting a monotropic cholesteric phase, and the 4-alkoxy-benzoic acid or 4-alkoxy cinnamic acid （proton donor）. It was found that the increase of the conjugate length as well as the terminal length can contribute to enhance the interaction of molecules and thus significantly influenced the thermal behaviors of H-bonded LCs. The cholesteric reflection spectra of the induced mesogenic complexes were located in the visible region with the color tuneable thermo-sensitivity, which could be used for display application.

关键词： Liquid crystal Hydrogen bond Self-assembly Selective reflection Thermo-chromism

Antitumor and antiangiogenic activities of anti-vascular endothelial growth factor hairpin ribozyme in human hepatocellular carcinoma cell cultures and xenografts

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World Journal of Gastroenterology 2007年第47期13卷 6425-6432页

作者： Li-Hua Li Zi-Jian Guo Ling-Ling Yan Ji-Cheng Yang Yu-Feng Xie Wei-Hua Sheng zhao-hui huang Xue-Hao Wang Oncology Institute of Wuxi the Fourth Affiliated Hospital of Soochow University 200 Huihe Road Wuxi 214062 Jiangsu Province China Department of Cellular and Molecular Biology Medical School Soochow University Soochow 215007 Jiangsu Province China Department of Liver and Biliary Duct Surgery the First Affiliated Hospital of Nanjing Medical University Nanjing 210029 Jiangsu Province China

AIM: To study the effectiveness and mechanisms of anti-human vascular endothelial growth factor (hVEGF) hairpin ribozyme on angiogenesis,oncogenicity and tumor growth in a hepatocarcinoma cell line and a xenografted model. METHODS: The artificial anti-hVEGF hairpin ribozyme was transfected into hepatocarcinoma cell line SMMC-7721 and,subsequently,polymerase chain reaction (PCR) and reverse transcription polymerase chain reaction (RT-PCR) were performed to confirm the ribozyme gene integration and transcription. To determine the effects of ribozyme ,VEGF expression was detected by semiquantitative RT-PCR and enzyme liked immunosorbent assay (ELISA). MTT assay was carried out to measure the cell proliferation. Furthermore,the transfected and control cells were inoculated into nude mice respectively,the growth of cells in nude mice and angiogenesis were observed. RESULTS: VEGF expression was down-regulated sharply by ribozyme in transfected SMMC-7721 cells and xenografted tumor. Compared to the control group,the transfected cells grew slower in cell cultures and xenografts,and the xenograft formation was delayed as well. In addition,the microvessel density of the xenografted tumor was obviously declined in the transfected group. As demonstratedby microscopy,reduction of VEGF production induced by ribozyme resulted in a significantly higher cell differentiation and less proliferation vigor in xenografted tumor. CONCLUSION: Anti-hVEGF hairpin ribozyme can effectively inhibit VEGF expression and growth of hepatocarcinoma in vitro and in vivo. VEGF is functionally related to cell proliferation,differentiation and tumori-genesis in hepatocarcinoma.

关键词： Vascular endothelial growth factor Angiogenesis Hairpin ribozyme Hepatocarcinoma Gene therapy

Glucose deprivation induces chemoresistance in colorectal cancer cells by increasing ATF4 expression

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World Journal of Gastroenterology 2016年第27期22卷 6235-6245页

作者： Ya-Ling Hu Yuan Yin He-Yong Liu Yu-Yang Feng Ze-Hua Bian Le-Yuan Zhou Ji-Wei Zhang Bo-Jian Fei Yu-Gang Wang zhao-hui huang Wuxi Oncology Institute Affiliated Hospital of Jiangnan University Department of Biologic and Materials Sciences University of Michigan School of Dentistry

AIM: To investigate the role of activating transcription factor 4(ATF4) in glucose deprivation(GD) induced colorectal cancer(CRC) drug resistance and the mechanism ***: Chemosensitivity and apoptosis were measured under the GD condition. Inhibition of ATF4 using short hairpin RNA in CRC cells under the GD condition and in ATF4-overexpressing CRC cells was performed to identify the role of ATF4 in the GD induced chemoresistance. Quantitative real-time RTPCR and Western blot were used to detect the mR NA and protein expression of drug resistance gene 1(MDR1), ***: GD protected CRC cells from drug-induced apoptosis(oxaliplatin and 5-fluorouracil) and induced the expression of ATF4, a key gene of the unfolded protein response. Depletion of ATF4 in CRC cells under the GD condition can induce apoptosis and drug resensitization. Similarly, inhibition of ATF4 in the ATF4-overexpressing CRC cells reintroduced therapeutic sensitivity and apoptosis. In addition, increased MDR1 expression was observed in GD-treated CRC cells. CONCLUSION: These data indicate that GD promotes chemoresistance in CRC cells through up-regulating ATF4 expression.

关键词： Glucose deprivation ATF4 Oxaliplatin 5-Fluorouracil Chemoresistance

Whole Lung Lavage Treatment of Chinese Patients with Autoimmune Pulmonary Alveolar Proteinosis： A Retrospective Long-term Follow-up Study

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Chinese Medical Journal 2015年第20期128卷 2714-2719页

作者： Yu-Yue zhao hui huang Yong-Zhe Liu Xin-Yu Song Shan Li Zuo-Jun Xu Department of Respiratory Medicine Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100730 China

Background： Pulmonary alveolar proteinosis （PAP） is a rare lung disease, the most common type of which is autoimmune PAP. The gold standard therapy for PAP is whole lung lavage （WLL）. Few studies have reported the optimal technique with which to evaluate the response to WLL. In this study, we aimed to identify parameters with which to assess the need for repeat WLL during a long-term 8-year follow-up. Methods： We conducted a retrospective analysis of 120 patients with autoimmune PAP with 80 of whom underwent WLL. Physiologic, serologic, and radiologic features of the patients were analyzed during an 8-year follow-up after the first WLL treatment. Results： Of the 40 patients without any intervention, 39 patients either achieved remission or remained stable and only one died of pulmonary infection. Of the 56 patients who underwent WLL for 1 time, 55 remained free from a second WLL and 1 patient died of cancer. Twenty-four required additional treatments after their first WLL. The baseline PaO2, （P = 0.000）, PA-aO2 （P = 0.000）, shunt fraction rate （P = 0.001）, percent of predicted normal diffusing capacity of the lung for carbon monoxide （DLCO%Pred）（P = 0.016）, 6-rain walk test （P = 0.013）, carcinoembryonic antigen （CEA）（P = 0.007）, and neuron-specific enolase （NSE）（P = 0.003） showed significant differences among the three groups. The need for a second WLL was significantly associated with PaO2 （P = 0.000）, CEA （P= 0.050）, the 6-minute walk test （P= 0.026）, and DLCO%Pred （P = 0.041 ）. The DLCO%Pred on admission with a cut-off value of42.1% （P = 0.001） may help to distinguish whether patients with PAP require a second WLL. Conclusions： WLL is the optimal treatment method for PAP and provides remarkable improvements for affected patients. The DLCO%Pred on admission with a cut-offvalue of 42.1% may distinguish whether patients with PAP require a second WLL.

关键词： Diffusing Capacity of the Lung fbr Carbon Monoxide Prognosis Pulmonary Alveolar Proteinosis Treatment:Whole Lung Lavage