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Asian Journal of Andrology 2024年第5期26卷 464-471页

作者： Qiu-Zi Shen Yong-Feng Wang yi-Wei Fang yuan-Yao Chen li-Ting He yuan zhang Guo-Tao liu Kai zhao Chun-Yan liu zun-Pan Fan Hui-Ping zhang Institute of Reproductive Health Center for Reproductive MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China The Affiliated Jinyang Hospital of Guizhou medical University Guiyang 550018China NHC Key Laboratory of Birth Defects Prevention Henan Institute of Reproduction Health Science and TechnologyZhengzhou 450002China Center for Reproductive Medicine The first Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China

Infections and inflammatory reactions in the male genital tract are the leading causes of male infertility with a prevalence of 6%-10%,primarily affecting testicular and epididymal function and ultimately compromising sperm ***,most infertile patients with genital infection/inflammation are asymptomatic and easily *** indicators,including white blood cells,elastase,and other components in semen,can reflect inflammation of the genital tract,but there is still a lack of a uniform standard method of ***,it is necessary to explore reliable markers in semen that reflect the inflammatory status of the genital *** the experimental autoimmune orchitis(EAO)model to simulate noninfectious chronic orchitis,we successfully collected ejaculated seminal fluid from EAO rats using optimized electrical stimulation *** analysis was performed using isobaric tags for relative and absolute quantification(iTRAQ).Compared to the control group,55 upregulated and 105 downregulated proteins were identified in seminal plasma samples from the EAO *** a preliminary screening,the inflammation-related protein S100A8/A9 was *** further verified that S100A8/A9 was increased in seminal plasma and highly expressed in testicular macrophages of the EAO *** patients with oligoasthenospermia and genital tract infections,we also found that S100A8/A9 levels were remarkably increased in seminal plasma and testicular macrophages.S100A8/A9 in semen may be a potential biomarker for chronic genital *** study provides a new potential biomarker for early diagnosis and further understanding of male infertility caused by genital inflammation.

关键词： experimental autoimmune orchitis genital inflammation male infertility proteomics S100A8/A9

Structural Revision of Isovalertatins D03 and D23 Isolated from the Culture Filtrate of Streptomyces luteogriseus

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Chinese Chemical Letters 2001年第4期12卷 327-330页

作者： duan yun SI Da Fang ZHONG Wen yi HE li Mei zhao Laboratory of Drug Metabolism and Pharmacokinetics Shenyang Pharmaceutical UniversityShenyang 110015 Center of Instrumental Analysis Institute of Materia MedicaChinese Academy of Medical ScienceBeijing 100050

Two aminooligosaccharides. isovalertatins D03 (1) and D23 (2). were isolated from the culture filtrate of Streptomyces luteogriseus. Their structures were reinvestigated and revised by spectroscopic evidences includin... 详细信息

关键词： Streptomyces luteogriseus aminooligosaccharide isovalertatin D03 isovalertatin D23.

In situ forming injectable MSC-loaded GelMA hydrogels combined with PD for vascularized sweat gland regeneration

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Military medical Research 2024年第1期11卷 152-155页

作者： Enhe Jirigala Bin Yao zhao li yi-Jie zhang Chao zhang li-Ting liang Fan-liang zhang xing-yu yuan Xian-Lan duan Wei Song Meng-De zhang yi Kong Xiao-Bing Fu Sha Huang Research Center for Tissue Repair and Regeneration Medical Innovation Research DepartmentChinese PLA General Hospital and PLA Medical CollegeBeijing 100048China Institute of Basic medical Research Inner Mongolia Medical UniversityHohhot 010110China Academy of medical Engineering and Translational Medicine Tianjin UniversityTianjin 300072China School of Medicine Nankai UniversityTianjin 300071China

Dear Editor,Three dimensional(3D)bioprinted extracellular matrix(ECM)can be used to provide both biochemical and biophysical cues to direct mesenchymal stem cells(MSCs)differentiation,and then differentiated cells were isolated for implantation in vivo using surgical ***,the reduced cell activity after cell isolation from 3D constructs and low cell retention in injured sites limit its application[1].Methacrylated gelatin(GelMA)hydrogel has the advantage of fast crosslinking,which could resemble complex architectures of tissue construct in vivo[2].Here,we adopted a noninvasive bioprinting procedure to imitate the regenerative microenvironment that could simultaneously direct the sweat gland(SG)and vascular differentiation from MSCs and ultimately promote the replacement of glandular tissue in situ(Fig.1a).

关键词： Sweat gland GelMA In situ niche Cell differentiation Tissue incorporation Vascularization

Optimal gamma-ray selections for monochromatic line searches with DAMPE

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Frontiers of physics 2022年第3期17卷 1-6页

作者： zun-Lei Xu Kai-Kai duan Wei Jiang Shi-Jun Lei Xiang li zhao-Qiang Shen Tao Ma Meng Su Qiang yuan Chuan yue yi-Zhong Fan Jin Chang Key Laboratory of Dark Matter and Space Astronomy Purple Mountain ObservatoryChinese Academy of SciencesNanjing 210023China School of Astronomy and Space Science University of Science and Technology of ChinaHefei 230026China

The DArk Matter Particle Explorer (DAMPE) is a space high-energy cosmic-ray detector covering a wide energy band with a high energy resolution. One of the key scientific goals of DAMPE is to carry out indirect detection of dark matter by searching for high-energy gamma-ray line structure. To promote the sensitivity of gamma-ray line search with DAMPE, it is crucial to improve the acceptance and energy resolution of gamma-ray photons. In this paper, we quantitatively proved that the photon sample with the largest ratio of acceptance to energy resolution is optimal for line search. We therefore developed a line-search sample specifically optimized for the line-search. Meanwhile, in order to increase the statistics, we also selected the so-called BGO-only photons that convert into e^(+)e^(-) pairs only in the BGO calorimeter. The standard, the line-search, and the BGO-only photon samples are then tested for line-search individually and collectively. The results show that a significantly improved limit could be obtained from an appropriate combination of the date sets, and the increase is about 20% for the highest case compared with using the standard sample only.

关键词： DArk Matter Particle Explorer(DAMPE) gamma-ray line-search

Adhesive hydrogels in osteoarthritis:from design to application

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Military medical Research 2023年第5期10卷 652-679页

作者： Wang-lin duan li-Ning zhang Raghvendra Bohara Sergio Martin-Saldana Fei Yang yi-Yang zhao Yong Xie Ya-Zhong Bu Abhay Pandit Institute of medical Engineering Department of BiophysicsSchoolof Basic Medical SciencesHealth Science CenterXi’an JiaotongUniversityXi’an 710061China Department of Rehabilitation Medicine the First Medical CenterChinese PLA General HospitalNo.28 Fuxing RoadHaidian DistrictBeijing 100853China CURAM SFI Research Centre for Medical DevicesUniversity of GalwayGalway H91 TK33Ireland Beijing National Laboratory for Molecular Sciences State Key Laboratoryof Polymer Physics and ChemistryInstitute of ChemistryChineseAcademy of SciencesBeijing 100190China School of Chemical Sciences University of Chinese Academyof SciencesBeijing 100049China Department of Orthopedics the Fourth Medical CenterChinese PLAGeneral HospitalBeijing 100853China National Clinical Research Center for Orthopedics Sports Medicineand RehabilitationBeijing 100853China

Osteoarthritis(OA)is the most common type of degenerative joint disease which affects 7%of the global population and more than 500 million people *** research frontier is the development of hydrogels for OA treatment,which operate either as functional scaffolds of tissue engineering or as delivery vehicles of functional *** approaches address the big challenge:establishing stable integration of such delivery systems or *** hydrogels provide possible solutions to this ***,few studies have described the current advances in using adhesive hydrogel for OA *** review summarizes the commonly used hydrogels with their adhesion mechanisms and ***,recognizing that OA is a complex disease involving different biological mechanisms,the bioactive therapeutic strategies are also *** presenting the adhesive hydrogels in an interdisciplinary way,including both the fields of chemistry and biology,this review will attempt to provide a comprehensive insight for designing novel bioadhesive systems for OA therapy.

关键词： Adhesive hydrogel Osteoarthritis Functional additives Cartilage regeneration Interdisciplinary therapy

Evaluation of triage strategies for high-risk human papillomavirus-positive women in cervical cancer screening:A multicenter randomized controlled trial in different resource settings in china

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Chinese Journal of Cancer Research 2022年第5期34卷 496-509页

作者： Le Dang linghua Kong yuqian zhao yi Dai li Ma lihui Wei Shulan zhang Jihong liu Mingrong Xi Long Chen Xianzhi duan Qing Xiao Guzhalinuer Abulizi Guonan zhang ying Hong Qi Zhou xing Xie li li Mayinuer Niyazi Zhifen zhang Jiyu Tuo yiling Ding Youlin Qiao Jinghe Lang Department of Gynecology and Obstetrics Peking Union Medical College HospitalChinese Academy of Medical Sciences/Peking Union Medical CollegeBeijing 100730China National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Center for Cancer Prevention Research Sichuan Cancer Hospital and InstituteSichuan Cancer CenterSchool of MedicineUniversity of Electronic Science and Technology of ChinaChengdu 610041China Department of Epidemiology Dalian Medical UniversityDalian 116044China Department Obstetrics and Gynecology Peking University People’s HospitalBeijing 100044China Department of Obstetrics and Gynecology Shengjing Hospital Affiliated to China Medical UniversityShenyang 110004China Department of Gynecology and Obstetrics Sun Yat-sen University Cancer CenterGuangzhou 510060China Department of Gynecology and Obstetrics West China Second University HospitalSichuan UniversityChengdu 610041China Department of Gynecology and Obstetrics Zhejiang Xiaoshan Hospital Affiliated to Hangzhou Normal UniversityChinese Academy of Medical Sciences/Zhejiang Xiaoshan Hospital Affiliated to Hangzhou Normal UniversityHangzhou 310000China Department of Obstetrics and Gynecology Beijing Tongren HospitalCapital Medical UniversityBeijing 100730China Department of Gynecology and Obstetrics the Eight Affiliated Hospital Sun Yat-sen UniversityShenzhen 518000China 5th Department of Gynecology the Affiliated Cancer Hospital of Xinjiang Medical UniversityUrumqi 830011China Department of Gynecologic Oncology Sichuan Cancer Hospital&InstituteSichuan Cancer CenterSchool of MedicineUniversity of Electronic Science and Technology of ChinaChengdu 610041China Department of Gynecology and Obstetrics Nanjing Drum Tower HospitalAffiliated Hospital of Nanjing University Medical SchoolNanjing 210008China Department of Gynecological Oncology Chongqing University Cancer Hospital/Chongqing Cancer HospitalCho

Objective:We aimed to evaluate the effectiveness of different triage strategies for high-risk human papillomavirus(hrHPV)-positive women in primary healthcare settings in ***:This study was undertaken in 11 rural and 9 urban *** aged 35-64 years old were ***-positive women were randomly allocated to liquid-based cytology(LBC),visual inspection with acetic acid and Lugol’s iodine(VIA/VIli)(rural only)triage,or directly referred to colposcopy(direct COLP).At 24 months,hrHPV testing,LBC and VIA/VIli were conducted for combined ***:In rural sites,1,949 hrHPV-positive women were analyzed.A total of 852,218 and 480 women were randomly assigned to direct COLP,LBC and VIA/*** baseline,colposcopy referral rates of LBC or VIA/VIli triage could be reduced by 70%-80%.LBC(n=3 and n=7)or VIA/VIli(n=8 and n=26)could significantly decrease the number of colposcopies needed to detect one cervical intraepithelial neoplasia(CIN)2 or worse and CIN3+compared with direct COLP(n=14 and n=23).For the 24-month cumulative detection rate of CIN2+,VIA/VIli triage was 0.50-fold compared with LBC triage and 0.46-fold with the direct *** stratified by age,baseline LBC triage+performed best(P<0.001),peaking among women aged 35-44 years(Ptrend=0.002).In urban sites,1,728 women were hrHPV genotyping test positive.A total of 408,571 and 568women were randomly assigned to direct COLP for HPV16/18+,direct COLP for other hrHPV subtypes+,and LBC triage for other hrHPV subtypes+.LBC(n=12 and n=31)significantly decreased the number of colposcopies needed to detect one CIN2+and CIN3+compared with direct COLP(n=14 and n=44).HPV16/18+increased the 24-month cumulative detection rate of CIN2+(17.89%,P<0.001).Conclusions:LBC triage for hrHPV-positive women in rural settings and direct COLP for HPV16/18+women and LBC triage for other hrHPV subtype+women in urban settings might be feasible strategies.

关键词： Cervical cancer screening hrHPV-positive LBC triage strategy VIA/VIli

Chronic spinal cord injury repair by NT3-chitosan only occurs after clearance of the lesion scar

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Signal Transduction and Targeted Therapy 2022年第7期7卷 2568-2580页

作者： Can zhao Jia-Sheng Rao Hongmei duan Peng Hao Junkui Shang yubo Fan Wen zhao yudan Gao zhaoyang Yang yi Eve Sun Xiaoguang li Beijing Key Laboratory for Biomaterials and Neural Regeneration Beijing Advanced Innovation Center for Biomedical EngineeringSchool of Biological Science and Medical EngineeringBeihang UniversityBeijing 100083China Institute of Rehabilitation Engineering China Rehabilitation Science InstituteBeijing 100068China Department of Neurobiology School of Basic Medical SciencesCapital Medical UniversityBeijing 100069China Beijing Advanced Innovation Centre for Biomedical Engineering Key Laboratory for Biomechanics and Mechanobiology of Chinese Education MinistrySchool of Biological Science and Medical EngineeringBeihang UniversityBeijing 10083China School of Engineering Medicine Beihang UniversityBeijing 10083China Shanghai Institute of Stem Cell Research and Clinical Translation Shanghai East HospitalTongji University School of MedicineShanghai 200120China Department of Psychiatry and Biobehavioral Sciences UCLA Medical SchoolLos AngelesCA 90095USA

Spinal cord injury(SCI)is a severe damage usually leading to limb dysesthesia,motor dysfunction,and other physiological *** have previously shown that NT3-chitosan could trigger an acute SCI repairment in rats and non-human *** to the negative effect of inhibitory molecules in glial scar on axonal regeneration,however,the role of NT3-chitosan in the treatment of chronic SCI remains *** with the fresh wound of acute SCI,how to handle the lesion core and glial scars is a major issue related to chronic-SCI *** we report,in a chronic complete SCI rat model,establishment of magnetic resonancediffusion tensor imaging(MR-DTI)methods to monitor spatial and temporal changes of the lesion area,which matched well with anatomical *** of the lesion core via suction of cystic tissues and trimming of solid scar tissues before introducing NT3-chitosan using either a rigid tubular scaffold or a soft gel form led to robust neural regeneration,which interconnected the severed ascending and descending axons and accompanied with electrophysiological and motor functional *** contrast,cystic tissue extraction without scar trimming followed by NT3-chitosan injection,resulted in little,if any *** together,after lesion core clearance,NT3-chitosan can be used to enable chronic-SCI repair and MR-DTI-based mapping of lesion area and monitoring of ongoing regeneration can potentially be implemented in clinical studies for subacute/chronic-SCI repair.

关键词： scar NT3 wound

Collagen triple helix repeat containing-1 promotes functional recovery of sweat glands by inducing adjacent microvascular network reconstruction in vivo

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Burns & Trauma 2022年第1期10卷 387-400页

作者： xingyu yuan Xianlan duan zhao li Bin Yao Enhejirigala Wei Song yi Kong yuzhen Wang Fanliang zhang liting liang Shijun Zhu Mengde zhang Chao zhang Sha Huang Xiaobing Fu School of Medicine Nankai University94 Wei Jin RoadTianjin 300071PR China Research Center for Tissue Repair and Regeneration affiliated to the medical Innovation Research Department PLA General Hospital28 Fu Xing RoadBeijing 100853PR China PLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin Injury Repair and RegenerationChinese PLA General Hospital and PLA Medical College51 Fu Cheng RoadBeijing 100048PR China Research Unit of Trauma Care Tissue Repair and RegenerationChinese Academy of Medical Sciences2019RU051Beijing 100048PR China Academy of medical Engineering and Translational Medicine Tianjin University92 Weijin RoadTianjin300072PR China college of Graduate Tianjin Medical UniversityTianjin 300070PR China Institute of Basic medical Research Inner Mongolia Medical UniversityHohhot 010110Inner MongoliaPR China Department of Burn and Plastic Surgery Air Force Hospital of Chinese PLA Central Theater CommandDatong 037000ShanxiPR China

Background:Sweat glands(SGs)have low regenerative potential after severe burns or trauma and their regeneration or functional recovery still faces many *** practice,restoring SG function requires not only the structural integrity of the gland itself,but also its neighboring tissues,especially blood *** triple helix repeat containing-1(CTHRC1)was first identified in vascular repair,and increasing reports showed a close correlation between cutaneous appendage specification,patterning and *** purpose of the present study was to clarify the role of CTHRC1 in SGs and their adjacent microvessels and find therapeutic strategies to restore SG ***:The SGs and their adjacent microvascular network of Cthrc^(1−/−)mice were first inves-tigated using sweat test,laser Doppler imaging,tissue clearing technique and transcriptome *** effects of CTHRC1 on dermal microvascular endothelial cells(DMECs)were further explored with cell proliferation,DiI-labeled acetylated low-density lipoprotein uptake,tube for-mation and intercellular junction establishment *** effects of CTHRC1 on SG function restoration were finally confirmed by replenishing the protein into the paws of Cthrc(1−/−)***:CTHRC1 is a key regulator of SG function in *** the tissue level,Cthrc1 deletion resulted in the disorder and reduction of the microvascular network around *** the molecular level,the knockout of Cthrc1 reduced the expression of vascular development genes and functional proteins in the dermal ***,CTHRC1 administration considerably enhanced SG function by inducing adjacent vascular network ***:CTHRC1 promotes the development,morphogenesis and function execution of SGs and their neighboring *** study provides a novel target for the restoration or regeneration of SG function in vivo.

关键词： Collagen triple helix repeat containing-1 Sweat glands Microvascular network Dermalmicrovascular endothelial cells Reconstruction

Reciprocal interaction between vascular niche and sweat gland promotes sweat gland regeneration

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Bioactive Materials 2023年第3期21卷 340-357页

作者： xingyu yuan Xianlan duan Enhejirigala zhao li Bin Yao Wei Song yuzhen Wang yi Kong Shijun Zhu Fanliang zhang liting liang Mengde zhang Chao zhang Deling Kong Meifeng Zhu Sha Huang Xiaobing Fu School of Medicine Nankai University94 Weijin RoadTianjin300071PR China Research Center for Tissue Repair and Regeneration Affiliated to the medical Innovation Research Department PLA General Hospital28 Fuxing RoadBeijing100853PR China PLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin Injury Repair and RegenerationChinese PLA General Hospital and PLA Medical College51 Fucheng RoadBeijing100048PR China college of Graduate Tianjin Medical UniversityTianjin300070PR China Institute of Basic medical Research Inner Mongolia Medical UniversityHohhot010110Inner MongoliaPR China Academy of medical Engineering and Translational Medicine Tianjin University92 Weijin RoadTianjin300072PR China Department of Burn and Plastic Surgery Air Force Hospital of Chinese PLA Central Theater CommandDatong037000ShanxiPR China college of life Science State Key Laboratory of Medicinal Chemical BiologyKey Laboratory of Bioactive Materials of Ministry of EducationNankai University94 Weijin RoadTianjin300071PR China Research Unit of Trauma Care Tissue Repair and RegenerationChinese Academy of Medical Sciences2019RU051Beijing100048PR China

The incorporation of vasculature is known to be effective in tissue or organ functional ***,a vague understanding of the interaction between epidermal appendages and their vascular niches is a foremost obstacle to obtaining sweat gland(SG)-specific vasculature ***,we map their precise anatomical con-nections and report that the interplay between SG cells(SGCs)and the surrounding vascular niche is key for glandular development and homeostasis *** replicate this interplay in vitro,we used three-dimensional(3D)bioprinting to generate reproducible SGC spheroids from differentiated adipose-derived mesenchymal stem cells(ADSCs).With dermal microvascular endothelial cells(DMECs),sacrificial templates made from poly(ε-caprolactone)(PCL)were fabricated to pattern the vascular *** interplay model promoted physiologically relevant vascularized glandular morphogenesis in vitro and in *** identified a reciprocal regulatory mechanism for promoting SGs regeneration via contact-independent cell communication and direct cell-cell interactions between SGs and the *** envision the successful use of our approach for vascularized organ regeneration in the near future.

关键词： Vascular niche 3D bioprinting Sweat gland-vasculature interaction model ECM scaffold Sweat gland regeneration