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Glutamatergic CYLD deletion leads to aberrant excitatory activity in the basolateral amygdala:association with enhanced cued fear expression

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Neural Regeneration Research 2025年第11期20卷 3259-3272页

作者： Huidong li Faqin li zhaoyi chen Erwen Wu Xiaoxi Dai Danni li Haojie An Shiyi Zeng Chunyan Wang li Yang cheng Long School of life Sciences South China Normal UniversityGuangzhouGuangdong ProvinceChina School of life Sciences Guangzhou UniversityGuangzhouGuangdong ProvinceChina Guangdong Provincial Key Laboratory of Major Obstetric Diseases The Third Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdong ProvinceChina South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research Panyu Central HospitalGuangzhouGuangdong ProvinceChina

Neuronal activity,synaptic transmission,and molecular changes in the basolateral amygdala play critical roles in fear ***(CYLD)is a deubiquitinase that negatively regulates the nuclear factor kappa-B *** is well studied in non-neuronal cells,yet underinvestigated in the brain,where it is highly *** studies have shown involvement of CYLD in the remodeling of glutamatergic synapses,neuroinflammation,fear memory,and anxiety-and autism-like ***,the precise role of CYLD in glutamatergic neurons is largely ***,we first proposed involvement of CYLD in cued fear *** next constructed transgenic model mice with specific deletion of Cyld from glutamatergic *** results show that glutamatergic CYLD deficiency exaggerated the expression of cued fear in only male ***,loss of CYLD in glutamatergic neurons resulted in enhanced neuronal activation,impaired excitatory synaptic transmission,and altered levels of glutamate receptors accompanied by over-activation of microglia in the basolateral amygdala of male ***,our study suggests a critical role of glutamatergic CYLD in maintaining normal neuronal,synaptic,and microglial *** may contribute,at least in part,to cued fear expression.

关键词： basolateral amygdala cued fear expression cylindromatosis deubiquitinase glutamate receptor 1 glutamatergic neuron microglial activation N-methyl-D-aspartate receptor 1 neuronal activation synaptic transmission

柯萨奇病毒腺病毒受体在非小细胞肺癌中的表达及其意义

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中华肿瘤杂志 2009年第4期31卷 278-281页

作者：顾安康孙蕾娜陈照立战忠利李君文天津市肿瘤防治重点实验室天津市肺癌诊治中心天津肿瘤医院肿瘤研究所病理科 300060 军事医学科学院卫生学环境医学研究所

目的研究柯萨奇病毒腺病毒受体（CAR）的mRNA和蛋白在正常肺组织、癌旁组织及肺癌组织中的表达情况，分析其与肺癌发生发展的相关性及在不同临床病理参数间的表达差异。方法应用半定量逆转录聚合酶链反应（RT—PCR）方法和Western blo... 详细信息

目的研究柯萨奇病毒腺病毒受体（CAR）的mRNA和蛋白在正常肺组织、癌旁组织及肺癌组织中的表达情况，分析其与肺癌发生发展的相关性及在不同临床病理参数间的表达差异。方法应用半定量逆转录聚合酶链反应（RT—PCR）方法和Western blot检测正常肺组织、癌旁组织和肺癌组织中CARmRNA和CAR蛋白的表达情况。结果CARmRNA和蛋白在正常肺组织、癌旁组织和肺癌组织中的表达量分别为1．000±0．012、1．048±0．035、1．282±0．072和0．902±0．038、0．944±0．042、1．08±0．052，其差异均有统计学意义（P〈0．05）。CAR mRNA和CAR蛋白在肺癌中的表达呈正相关（r=0．448，P=0．026）。在不同的临床病理参数之间，CAR mRNA和蛋白的表达在患者性别、年龄、吸烟与否、肿瘤大小、有无淋巴结转移及TNM分期中差异无统计学意义（P〉0．05）；CAR的表达在不同的病理类型中差异具有统计学意义（P：0．012），在7例细支气管肺泡癌（BAC）中均高表达（P=0．029）。结论肺癌组织中CAR的表达水平显著高于正常肺组织和癌旁组织，且在不同病理类型的表达上存在差异，提示CAR在肺癌的发生发展过程中起到了重要作用，并可能成为潜在的肿瘤标记及预后指标。

关键词：柯萨奇病毒腺病毒受体癌非小细胞肺印迹法蛋白质

Molecule‑Level Multiscale Design of Nonflammable Gel Polymer Electrolyte to Build Stable SEI/CEI for lithium Metal Battery

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Nano-Micro Letters 2025年第1期17卷 404-423页

作者： Qiqi Sun Zelong Gong Tao Zhang Jiafeng li Xianli Zhu Ruixiao Zhu lingxu Wang Leyuan Ma Xuehui li Miaofa Yuan Zhiwei Zhang Luyuan Zhang zhao Qian Longwei Yin Rajeev Ahuja chengxiang Wang Key Laboratory for liquid‑Solid Structural Evolution and Processing of Materials(Ministry of Education) School of Materials Science and EngineeringShandong UniversityJinan 250061People’s Republic of China Condensed Matter Theory Department of Physics and AstronomyUppsala UniversityUppsala 75120Sweden

The risk of flammability is an unavoidable issue for gel polymer electrolytes(GPEs).Usually,flameretardant solvents are necessary to be used,but most of them would react with anode/cathode easily and cause serious interfacial instability,which is a big challenge for design and application of nonflammable ***,a nonflammable GPE(SGPE)is developed by in situ polymerizing trifluoroethyl methacrylate(TFMA)monomers with flame-retardant triethyl phosphate(TEP)solvents and liTFSI–liDFOB dual lithium *** is strongly anchored to PTFMA matrix via polarity interaction between-P=O and-CH_(2)CF_(3).It reduces free TEP molecules,which obviously mitigates interfacial reactions,and enhances flame-retardant performance of TEP *** TEP molecules are also inhibited in solvation of li^(+),leading to anion-dominated solvation sheath,which creates inorganic-rich solid electrolyte interface/cathode electrolyte interface *** coordination structure changes li^(+)transport from sluggish vehicular to fast structural transport,raising ionic conductivity to 1.03 mS cm^(-1) and transfer number to 0.41 at 30℃.The li|SGPE|li cell presents highly reversible li stripping/plating performance for over 1000 h at 0.1 mA cm^(−2),and 4.2 V liCoO_(2)|SGPE|li battery delivers high average specific capacity>120 mAh g^(−1) over 200 *** study paves a new way to make nonflammable GPE that is compatible with li metal anode.

关键词： Anchoring effect Nonflammable gel electrolyte In situ cross-linked Electrode-electrolyte interface li metal battery

CDK8在宫颈癌及宫颈上皮内瘤变中的表达及意义

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中国妇产科临床杂志 2018年第3期19卷 232-234页

作者：张利梅陈丽夏敏赵磊重庆市中医院妇科 400021 重庆市中医院心内科 400021

目的探索细胞周期依赖性蛋白激酶8（CDK8）的表达水平与宫颈癌及宫颈上皮内瘤变（CIN）发生的关系。方法选取重庆市中医院就诊并进行宫颈组织活检的患者221例，其中宫颈癌48例，CINl37例，正常或炎症36例，免疫组化法检测CDK8的表达；... 详细信息

目的探索细胞周期依赖性蛋白激酶8（CDK8）的表达水平与宫颈癌及宫颈上皮内瘤变（CIN）发生的关系。方法选取重庆市中医院就诊并进行宫颈组织活检的患者221例，其中宫颈癌48例，CINl37例，正常或炎症36例，免疫组化法检测CDK8的表达；同时检测P53、Ki67蛋白表达，探索CDK8表达与癌及CIN发生的相关性。结果各组患者宫颈组织中的CDK8表达存在显著差异（P〈0．05），其中癌症组表达水平最高；CIN患者CDK8表达水平CIN3〉CIN2〉CINl，差异有显著性（P〈0．05）；随访中，治愈组和病变持续组CINl患者CDK8表达无显著差异（P〉0．05）；而在CIN2和CIN3病变持续的患者中，CDK8表达显著高于治愈组（P〈0．05）；在癌症组织中，CDK8的表达与P53及Ki67的表达均呈现正相关（r=0．64，r=0．57，P〈0．05）。结论CDK8表达与宫颈癌的发生及CIN病变、转归相关，其中在宫颈癌组织中Ki-67及P53蛋白的表达，或许与CDK8的表达存在着协同作用。

关键词： CDK8 细胞周期宫颈癌宫颈上皮内瘤变

Efficient and Stable Photoassisted lithium‑Ion Battery Enabled by Photocathode with Synergistically Boosted Carriers Dynamics

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Nano-Micro Letters 2025年第3期17卷 440-454页

作者： Zelin Ma Shiyao Wang Zhuangzhuang Ma Juan li Luomeng zhao Zhihuan li Shiyuan Wang Yazhou Shuang Jiulong Wang Fang Wang Weiwei Xia Jie Jian Yibo He Junjie Wang Pengfei Guo Hongqiang Wang State Key Laboratory of Solidification Processing Center for Nano Energy MaterialsSchool of Materials Science and EngineeringNorthwestern Polytechnical University and Shaanxi Joint Laboratory of Graphene(NPU)Xi’an 710072People’s Republic of China State Key Laboratory of Solidification Processing School of Materials Science and Engineering DepartmentNorthwestern Polytechnical UniversityXi’an 710072People’s Republic of China State Key Laboratory of Solidification Processing Center of Advanced Lubrication and Seal MaterialsSchool of Materials Science and EngineeringNorthwestern Polytechnical UniversityXi’an 710072People’s Republic of China Research and Development Institute of Northwestern Polytechnical University in Shenzhen Shenzhen 518063People’s Republic of China Key Laboratory of Applied Surface and Colloid Chemistry Shaanxi Key Laboratory for Advanced Energy DevicesShaanxi Engineering Lab for Advanced Energy TechnologySchool of Materials Science and EngineeringNational Ministry of EducationShaanxi Normal UniversityXi’an 710119People’s Republic of China

Efficient and stable photocathodes with versatility are of significance in photoassisted lithium-ion batteries(PliBs),while there is always a request on fast carrier transport in electrochemical active *** work proposes a general approach of creating bulk heterojunction to boost the carrier mobility of photocathodes by simply laser assisted embedding of plasmonic *** employed in PliBs,it was found effective for synchronously enhanced photocharge separation and transport in light charging ***,experimental photon spectroscopy,finite difference time domain method simulation and theoretical analyses demonstrate that the improved carrier dynamics are driven by the plasmonic-induced hot electron injection from metal to TiO_(2),as well as the enhanced conductivity in TiO2 matrix due to the formation of oxygen vacancies after Schottky *** from these merits,several benchmark values in performance of TiO2-based photocathode applied in PliBs are set,including the capacity of 276 mAh g^(−1)at 0.2 A g^(−1)under illumination,photoconversion efficiency of 1.276%at 3 A g^(−1),less capacity and Columbic efficiency loss even through 200 *** results exemplify the potential of the bulk heterojunction strategy in developing highly efficient and stable photoassisted energy storage systems.

关键词： Photoassisted lithium-ion batteries Bulk heterojunction Carrier dynamics TiO2 nanofiber Plasmonic metal nanocrystals

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硼对黑木相思幼苗生长发育的影响

林业科学研究 2023年第6期36卷 181-191页

作者：陈朝黎白晓刚李湘阳曾炳山胡冰中国林业科学研究院热带林业研究所热带林业研究国家林业和草原局重点实验室广东广州510520 仲恺农业工程学院农业与生物学院广东广州510225

[目的]研究不同供硼量培养条件下,黑木相思生长指标、生理生化特性和营养元素含量的变化,揭示黑木相思对硼胁迫环境的响应模式。[方法]以1月龄黑木相思无性系SR17幼苗为材料,利用含0、0.1、1、2 mmol·L^(-1)硼酸的营养液培养2个月后,... 详细信息

[目的]研究不同供硼量培养条件下,黑木相思生长指标、生理生化特性和营养元素含量的变化,揭示黑木相思对硼胁迫环境的响应模式。[方法]以1月龄黑木相思无性系SR17幼苗为材料,利用含0、0.1、1、2 mmol·L^(-1)硼酸的营养液培养2个月后,测定黑木相思的生长指标、叶绿素含量、生理活性物质含量、氧化还原酶活性和营养元素含量等生理生化指标的变化,基于主成分与隶属函数分析,综合评价各指标对黑木相思响应不同供硼量的贡献率,比较黑木相思幼苗对不同供硼量的适应性。[结果]试验结果表明,0、1和2 mmol·L^(-1)供硼量抑制了黑木相思幼苗株高和根长的增加,降低了地上部分叶绿素的含量,引起叶片失绿、黄化甚至脱落的表型。0 mmol·L^(-1)供硼量使黑木相思的主根和侧根呈白色且增粗,1、2 mmol·L^(-1)供硼量使根系褐化。0、1、2 mmol·L^(-1)供硼量降低了黑木相思体内抗坏血酸(AsA)的含量,引起了氧化反应;增加了丙二醛(MDA)和脯氨酸(Pro)含量,引起体内脂质氧化水平升高和渗透胁迫;增加了体内超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、脂氧合酶(LOX)和苯丙氨酸解氨酶(PAL)的活性,引发了氧自由基和过氧化氢(H_(2)O_(2))的积累与清除以及次生代谢物的合成。黑木相思体内硼元素含量增加,磷和钾元素的含量也随之增加,而钙元素含量在0.1 mmol·L^(-1)供硼量下最高。通过主成分和隶属函数分析,钾元素含量、叶片数增长量、株高增长量、LOX活性、AsA含量、钙元素含量、硼元素含量、POD活性、Pro含量、SOD活性、根长增长量、蛋白浓度、PAL活性及叶绿素b含量14个指标可作为黑木相思生长发育响应硼胁迫的主要指标。[结论]黑木相思幼苗在0.1mmol·L^(-1)供硼量下生长势较好,其生长指标、生理生化特性和营养元素含量在不同供硼量条件下差异显著,黑木相思通过调控植株表型、生理活性物质含量、氧化还原酶活性和营养元素含量响应硼胁迫。

关键词：黑木相思硼胁迫营养元素主成分分析生长发育

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大鼠视网膜神经细胞的原代培养(英文)

国际眼科杂志 2012年第1期12卷 4-6页

作者：阴杰平孟岩陈召利青岛大学医学院附属医院眼科中国山东省青岛市266003

目的:在前人建立的方法上优化SD大鼠视网膜神经细胞体外培养的技术和方法,为后续研究提供实验基础。方法:使用胰酶消化法分离新生1～3dSD大鼠视网膜神经细胞,以DMEM/F12为培养基体外培养,免疫组织化学染色的方法进行鉴定。结果:观察光... 详细信息

目的:在前人建立的方法上优化SD大鼠视网膜神经细胞体外培养的技术和方法,为后续研究提供实验基础。方法:使用胰酶消化法分离新生1～3dSD大鼠视网膜神经细胞,以DMEM/F12为培养基体外培养,免疫组织化学染色的方法进行鉴定。结果:观察光镜下培养的细胞贴壁生长,部分细胞伸出突起,且有些突起相互连接。免疫细胞化学染色显示,培养的细胞大多数抗神经元特异性烯醇化酶(neuron specific enolase,NSE)抗体反应阳性。结论:视网膜神经细胞体外培养成功为进一步进行视网膜疾病的研究创造了条件。

关键词：细胞培养视网膜神经元胰蛋白酶消化法神经元特异性烯醇化酶

The complex roles of m^(6)A modifications in neural stem cell proliferation, differentiation, and self-renewal and implications for memory and neurodegenerative diseases

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Neural Regeneration Research 2025年第6期20卷 1582-1598页

作者： Yanxi li Jing Xue Yuejia Ma Ke Ye Xue zhao Fangliang Ge Feifei Zheng Lulu liu Xu Gao Dayong Wang Qing Xia Department of Biochemistry and Molecular Biology College of Basic Medical SciencesHarbin Medical UniversityHarbinHeilongjiang ProvinceChina College of Basic Medical Sciences Harbin Medical UniversityHarbinHeilongjiang ProvinceChina Basic Medical Institute Heilongjiang Academy of Medical SciencesHarbinHeilongjiang ProvinceChina Key Laboratory of Heilongjiang Province for Genetically Modified Animals Harbin Medical UniversityHarbinHeilongjiang ProvinceChina Translational Medicine Research and Cooperation Center of Northern China Heilongjiang Academy of Medical SciencesHarbinHeilongjiang ProvinceChina Dongzhimen Hospital Beijing University of Chinese MedicineBeijingChina

N6-methyladenosine(m^(6)A), the most prevalent and conserved RNA modification in eukaryotic cells, profoundly influences virtually all aspects of mRNA metabolism. mRNA plays crucial roles in neural stem cell genesis and neural regeneration, where it is highly concentrated and actively involved in these processes. Changes in m^(6)A modification levels and the expression levels of related enzymatic proteins can lead to neurological dysfunction and contribute to the development of neurological diseases. Furthermore, the proliferation and differentiation of neural stem cells, as well as nerve regeneration, are intimately linked to memory function and neurodegenerative diseases. This paper presents a comprehensive review of the roles of m^(6)A in neural stem cell proliferation, differentiation, and self-renewal, as well as its implications in memory and neurodegenerative diseases. m^(6)A has demonstrated divergent effects on the proliferation and differentiation of neural stem cells. These observed contradictions may arise from the time-specific nature of m^(6)A and its differential impact on neural stem cells across various stages of development. Similarly, the diverse effects of m^(6)A on distinct types of memory could be attributed to the involvement of specific brain regions in memory formation and recall. Inconsistencies in m^(6)A levels across different models of neurodegenerative disease, particularly Alzheimer's disease and Parkinson's disease, suggest that these disparities are linked to variations in the affected brain regions. Notably, the opposing changes in m^(6)A levels observed in Parkinson's disease models exposed to manganese compared to normal Parkinson's disease models further underscore the complexity of m^(6)A's role in neurodegenerative processes. The roles of m^(6)A in neural stem cell proliferation, differentiation, and self-renewal, and its implications in memory and neurodegenerative diseases, appear contradictory. These inconsistencies may be a

关键词： Alzheimer's disease cell self-renewal central nervous system memory microglia nerve regeneration neurodegenerative diseases neurogenesis RNA methylation

The cGAS-STING-interferon regulatory factor 7 pathway regulates neuroinflammation in Parkinson's disease

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Neural Regeneration Research 2025年第8期20卷 2361-2372页

作者： Shengyang Zhou Ting li Wei Zhang Jian Wu Hui Hong Wei Quan Xinyu Qiao Chun Cui chenmeng Qiao Weijiang zhao Yanqin Shen Laboratory of Neurodegenerative and Neuroinjury Diseases Wuxi Medicine SchoolJiangnan UniversityWuxiJiangsu ProvinceChina

Interferon regulatory factor 7 plays a crucial role in the innate immune ***,whether interferon regulatory factor 7-mediated signaling contributes to Parkinson's disease remains *** we report that interferon regulatory factor 7 is markedly up-regulated in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced mouse model of Parkinson's disease and co-localizes with microglial *** the selective cyclic guanosine monophosphate adenosine monophosphate synthase inhibitor RU.521 and the stimulator of interferon genes inhibitor H151 effectively suppressed interferon regulatory factor 7 activation in BV2 microglia exposed to 1-methyl-4-phenylpyridinium and inhibited transformation of mouse BV2 microglia into the neurotoxic M1 *** addition,si RNA-mediated knockdown of interferon regulatory factor 7 expression in BV2 microglia reduced the expression of inducible nitric oxide synthase,tumor necrosis factorα,CD16,CD32,and CD86 and increased the expression of the anti-inflammatory markers ARG1 and *** together,our findings indicate that the cyclic guanosine monophosphate adenosine monophosphate synthase-stimulator of interferon genes-interferon regulatory factor 7 pathway plays a crucial role in the pathogenesis of Parkinson's disease.

关键词： cyclic guanosine monophosphate adenosine monophosphate synthase H151 interferon regulatory factor 7 M1 phenotype neurodegenerative disease neuroinflammation Parkinson’s disease RU521 STING type I interferon