检索结果-南通市图书馆

生物物理学报 2009年第S1期25卷 248-249页

作者： zhao yanting1, zhou peng1, {2. {3. ji guangju2. wang shiqiang1 1state key Laboratory of Biomembrane and membrane {12.,{13., peking university, and 2.nstitute of biophysics, chinese academy of sciences, being, china, 100871

FK506 binding protein 12.6 (FKBP12.6) is the protein in the ryanodine receptor complex, but its function is controversial in different reported work. Some conclude that FKBP12.

关键词： β-adrenergic FKBP12.6 isoproterenol ryanodine receptor spark

FKBP12.6 Knockout Increases Ryanodine Receptors Open Probability During β-adrenergic Stimulation in Mice Cardiac Myocytes

在线全文

学校读者我要写书评

暂无评论

FKBP12.6 Knockout Increases Ryanodine Receptors Open Probabi...

FKBP12.6 Knockout Increases Ryanodine Receptors Open Probability during b-adrenergic Stimulation in Mice Cardiac Myocytes

第十一次中国生物物理学术大会暨第九届全国会员代表大会

作者： zhao yanting1,zhou peng 1,{3. zheng 2,ji guangju 2,wang shiqiang 1 1state key Laboratory of Biomembrane and membrane {13.,college of life sciences,peking university,and 2.nstitute of biophysics,chinese academy of sciences,being,china,100871

＜正＞FK506 binding protein 12.6（FKBP12.6）is the protein in the ryanodine receptor complex,but its function is controversial in different reported *** conclude that FKBP12.6

关键词： β-adrenergic FKBP12.6 isoproterenol ryanodine receptor spark

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

FKBP12-6 Knockout Increases Ryanodine Receptors Open Probabi...

Identification of propranolol and derivatives that are chemical inhibitors of phosphatidate phosphatase as potential broad-spectrum fungicides

“基因、进化与生理功能多样性”海内外学术研讨会暨中国生理学会第七届比较生理学学术会议

作者： Yan-Ting zhao~1,peng zhou~1,{3. chen~2,Guang-Ju ji~2,Shi-Qiang wang~1 1 state key Laboratory of Biomembrane and membrane biotechnology,college of {13. sciences, peking university 2 Institute of biophysics,chinese academy of sciences,Beijing 100871

FK506 binding protein 12.6（FK-BP12.6） is the protein in the ryanodine receptor complex,but its function is controversial in different reported *** conclude that FK-BP12.6 stabilizes ryanodine receptors（RyRs） but some conclude that it has no effect on *** published work of FKBP12.6 effect on RyRs is demonstrated by lipid bilayer experiment,which destroys the in situ environment around FKBP12. 6 and ***,we employed two methods to test FKBP12.6 function directly in RyR complex in intact cardiac ***,using loose-seal patch clamp combined with confocal imaging,we gave the cell membrane a local depolarization to～0mV just below the pipette,at the same time recorded the Ca fluorescent signals,as sparklet from L-type calcium channel（LCC） and spark from *** found that the spark amplitude was decreased and the LCC-RyR coupling latency was shortened in FKBP12.6 knockout mice （KO）.Analyzation of spark flux revealed that the quantal nature of RyR Ca release observed in WT was diminished in KO,indicating that the coupled state of RyR Ca release channel was destroyed when FKBP12.6 was dissociated from RyR *** using isoproterenol（ISO）, a b-adrenergic receptors agonist,we found that the LCC-RyR coupling latency in KO myocytes was much shorter,more sparks and Ca waves occurred compared with ISO treated WT myocytes. We also certified the above results in rat cardiac myocytes pharmacologically,using FK506,the drug proved to dissociate FKBP12.6 from RyR ***,using whole cell patch clamp with confocal imaging,we gave cells a high voltage depolarization（+ 160mV） to make sure all the available LCCs were open,then repolarized to different voltages（-70-12. mV） to make different *** found that FK506 with ISO together induced higher Ca transient, whatever i was small（at higher repolarization voltages） or big（at lower repolarization voltages）. Together,we conclude that FKBP12.6 is important to maintain the quantal nature of RyR Ca release channe

关键词： B-adrenergic FKBP12.6 Isoproterenol Ryanodine receptor Spark

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Plant Communications 2024年第1期5卷 215-230页

作者： Juan zhao Yu chen Zhifen Ding Yaru {2. Ruiqing Bi Ziting Qin Lei Yang {3. Sun Qiping Sun Guang chen Daiyuan Sun Xianya jiang Lu zheng Xiao-Lin chen Hu Wan Guanghui wang Qiang Li Huailong Teng Guotian Li National key Laboratory of Agricultural Microbiology Hubei Hongshan LaboratoryHubei Key Laboratory of Plant PathologyThe Center of Crop NanobiotechnologyHuazhong Agricultural UniversityWuhan 430070China college of Science Huazhong Agricultural UniversityWuhan 430070China National key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China state key Laboratory of Crop Stress Biology for Arid Areas College of Plant ProtectionNorthwest A&F UniversityYangling 712100China college of Chemistry and life {2. Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological ResourcesChengdu Normal UniversityChengdu 611130China Yangjiang Institute of Agricultural {2. Yangjiang 529500China

Plant diseases cause enormous economic losses in agriculture and threaten global food security,and application of agrochemicals is an important method of crop disease *** of disease-resis-tance mechanisms and synthesis of highly bioactive agrochemicals are thus important research ***,we show that propranolol,a phosphatidate phosphatase(Pah)inhibitor,effectively suppresses fungal growth,sporulation,sexual reproduction,and infection of diverse *** MoPah1 enzyme activity of the rice blast fungus Magnaporthe oryzae is inhibited by *** in lipid metabolism are associated with inhibited hyphal growth and appressorium formation caused by propranolol in *** inhibits a broad spectrum of 12.plant pathogens,effectively inhibiting infection of barley,wheat,maize,tomato,and *** improve antifungal capacity,we synthesized a series of propranolol derivatives,one of which shows a 16-fold increase in antifungal ability and binds directly to *** and its derivatives can also reduce the severity of rice blast and Fusarium head blight of wheat in *** together,our results demonstrate that propranolol suppresses fungal development and infection through mechanisms involved in lipid *** and its derivatives may therefore be promising candidates for fungicide development.

关键词： propranolol molecular design chemical modification virulence lipid metabolism Pyricularia oryzae

FKBP12.6 is required for coupled-gating of ryanodine receptors and stabilized Ca2. signaling in intact cardiomyocytes

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

FKBP12.6 is required for coupled-gating of ryanodine recepto...

Clinical manifestations,diagnosis and long-term prognosis of adult autoimmune enteropathy:Experience from peking Union Medical college Hospital

中国生理学会第2.届全国会员代表大会暨生理学学术大会

作者： Yan-Ting zhao~1,peng zhou~1,{3.ng-Chao LI~1,zheng chen~2,Guang-Ju {3.~2,Shi-Qiang wang~1 1 Store key Laboratory of Biomembrane and membrane biotechnology,college of {13. sciences,peking university,Bei{3.ng 100871, 2 Institute of biophysics,chinese academy of sciences,Beijng 100101,china

In heart cells,FK506 binding protein 12.6(FKBP12.6) binds to the ryanodine receptor(RyR2. Ca release Channels,but its role in modifying RyR function has been debated for *** of the reasons for the controversies is that all the {3. experiments about FKBP12.6-RyR relationship are done in vitro with the experimental settings differing from lab to ***,we investigated the role FKBP12.6 in RyR regulation in intact heart cells by the aid of FKBP12.6-knockout(FKO) mice and FK506 *** the loose-seal patch-clamp conditions,in-focus Ca sparks from RyRs were triggered *** showed that the release fluxes underlying Ca sparks exhibited quantal distributions,with most Ca sparks corresponding to 2.4 *** contrast,the quantal property of RyR Ca release fluxes was absent in both FKO cells and in wild type cells treated with FK506,indicating that coupled gating of multiple RyRs were basically eliminated by the dissociation of FKBP12.6. To demonstrate the functional consequence of FKBP12.6 dissociation and RyR decoupling,we examined the RyR response to a single L-type Ca channel(LCC).We found that the LCC-RyR coupling latency was shortened in both FKO cells and FK506-treated cells,indicating that the uncoupled RyRs became more sensitive to LCC Ca ***,our results provide unequivocal in situ evidence that FKBP12.6 does mediate the coupled gating of RyRs in a dynamic *** coupled gating limits the RyR sensitivity of Ca-induced Ca release,and plays an important role in stabilizing intracellular Ca signaling.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2024年第19期30卷 2523-2537页

作者： Mu-Han Li Ge-Chong Ruan Wei-Xun zhou Xiao-Qing Li Sheng-Yu Zhang Yang chen Xiao-Yin Bai Hong Yang Yu-{2.e Zhang {3.-Yu zhao {2. Li {2.ng-Nan Li Department of Gastroenterology Chinese Academy of Medical Sciences&Peking Union Medical College HospitalBeijing 100730China Department of Pathology Chinese Academy of Medical Scinences&Peking Union Medical College HospitalBeijing 100730China Department of Pathology Tianjin First Central HospitalNankai UniversityTianjin 300192China Affairs Office Chinese Academy of Medical Sciences&Peking Union Medical College Hospital(West campus)Beijing 100032China

BACKGROUND Autoimmune enteropathy(AIE)is a rare disease whose diagnosis and long-term prognosis remain challenging,especially for adult AIE *** To improve overall understanding of this disease’s diagnosis and *** We retrospectively analyzed the clinical,endoscopic and histopathological characteristics and prognoses of 16 adult AIE patients in our tertiary medical center between 2.11 and 2.2.,whose diagnosis was based on the 2.07 diagnostic *** Diarrhea in AIE patients was characterized by secretory *** common endoscopic manifestations were edema,villous blunting and mucosal hyperemia in the duodenum and *** blunting(100%),deep crypt lymphocytic infiltration(67%),apoptotic bodies(50%),and mild intraepithelial lymphocytosis(69%)were observed in the duodenal ***,there were other remarkable abnormalities,including reduced or absent goblet cells(duodenum 94%,ileum 62.),reduced or absent Paneth cells(duodenum 94%,ileum 69%)and neutrophil infiltration(duodenum 100%,ileum 69%).Our patients also fulfilled the 2.18 diagnostic criteria but did not match the 2.2. diagnostic criteria due to undetectable anti-enterocyte *** patients received glucocorticoid therapy as the initial medication,of which 14/16 patients achieved a clinical response in 5(IQR:3.2.)*** were administered to 9 patients with indications of steroid dependence(6/9),steroid refractory status(2.9),or intensified maintenance medication(1/9).During the median of 2..5 months of followup,2.patients died from multiple organ failure,and 1 was diagnosed with non-Hodgkin’s *** cumulative relapse-free survival rates were 62.5%,55.6%and 3..0%at 6 months,12.months and 48 months,*** Certain histopathological findings,including a decrease or disappearance of goblet and Paneth cells in intestinal biopsies,might be potential diagnostic criteria for adult *** long-term prognosis is still unsatisfac

关键词： Autoimmune enteropathy Clinical manifestations Diagnostic criteria Pathological features Long-term prognosis

Multidisciplinary and multiscale nanoscience research roadmap based on large scientific facilities

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science china Chemistry 2024年第8期67卷 2497-2523页

作者： Yuan Zeng Shuhan Zhang Yuecong Guo Yalin Cong Xu Ding Peihua Li Yunxiang Lin Wenzhi Ren Hui Su Weigang Sun Liuxin Xu Guikai Zhang Shihua chen Yu chen Weiren cheng Shengqi Chu Yong Guan {3.nru Han {3.e Lin Heng{3.e Liu Zheyi Liu Pan Luo Fanchun Meng Sicong Qiao Zongyin Song Ying wang zhao Wu chenyu Yang Meng Yang Shirui Yang Zi Yin Zhibin Yin pengjun Zhang Hongyu Zhang Pingping zheng {3.a zhou Wanlin zhou pengfei An He cheng Chunhai Fan Xing{3.u Huang Yong Lei Lina Li Mu Li Qinghua Liu Shuming peng Li Song Zhihu Sun Yangchao Tian Fangjun wang Lihua wang Liming wang shiqiang Wei Aiguo Wu Chunlei Xiao Xueming Yang Panchao Yin {3.ng Zhang Mingxin Zhang Yaling wang Chunying chen CAS key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety New Cornerstone Science LaboratoryNational Center for Nanoscience and TechnologyBeijing 100190China university of chinese academy of sciences Beijing 100049China National Synchrotron Radiation Laboratory University of Science and Technology of ChinaHefei 230029China Institute of Solid state Physics Hefei Institutes of Physical ScienceChinese Academy of SciencesHefei 230031China Institutes of Physical Science and Information Technology Anhui UniversityHefei 230601China Ningbo key Laboratory of Biomedical Imaging Probe Materials and Technology Zhejiang International Cooperation Base of Biomedical Materials Technology and ApplicationCAS Key Laboratory of Magnetic Materials and DevicesNingbo Cixi Institute of Biomedical EngineeringZhejiang Engineering Research Center for Biomedical MaterialsNingbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingbo 315201China Advanced Energy Science and Technology Guangdong Laboratory Huizhou 516000China college of Chemistry and Chemical Engineering Hunan Normal UniversityChangsha 410081China state key Laboratory of Luminescent Materials and Devices South China Advanced Institute for Soft Matter Science and TechnologySouth China University of TechnologyGuangzhou 510640China Beijing Synchrotron Radiation Facility Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China CAS key Laboratory of Separation sciences for Analytical Chemistry Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China state key Laboratory of Molecular Reaction Dynamics Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China Institute of Advanced Science Facilities Shenzhen 518107China state key Laboratory of Coal Conversion Institute of Coal ChemistryChinese Academy of SciencesTaiyuan 030001China state key Laboratory of Particle Detection and Electronics Institute of High Energy PhysicsChinese Acade

With the advancement of modern science and technology, large scientific facilities are increasingly oriented toward demand and application, and can be used for basic research as well as serving multiple disciplines. Developing large scientific facilities and related analytical technologies enhances understanding of large scientific facilities and popularizes their application in research across multiple disciplines. The combination of light or neutron sources from large scientific facilities and advanced analytical technologies can be achieved for materials structure information, dynamics study of chemical reactions, high dissociation of biomolecules, 3. visualization of energy materials or biological samples, etc. We first introduce the progress of domestic large scientific facilities of synchrotron radiation(SR) and free electron lasers(FELs) with different wavelengths and neutron *** further discuss the comparison between chinese and typical foreign facilities in X-ray radiation from X-ray tubes, synchrotrons, X-ray FELs, and neutron sources based on physical parameters of light and neutron sources. In addition, we focus on the technological progress and perspectives combined with advanced X-ray radiation and neutron sources of large scientific facilities in china, especially in the nanoscience fields of energy catalysis and biological science. We hope that this roadmap will provide references on technology and methods to experimental users, as well as prospects for future development of technologies based on large research infrastructure facilities. Comprehensive studies and guidelines for basic research to practical application in various disciplines can be made with the assistance of large scientific facilities.

关键词： large scientific facilities synchrotron radiation FEL light sources neutron sources spectroscopic analysis methods X-ray imaging techniques structural information chemical dynamics

Genome assembly of autotetraploid Actinidia arguta highlights adaptive evolution and enables dissection of important economic traits

在线全文

学校读者我要写书评

暂无评论

Plant Communications 2024年第6期5卷 173-192页

作者： Xue-Mei Lu Xiao-Fen Yu Guo-Qiang Li Ming-Hao Qu Huan wang Chuang Liu Yu-Ping Man Xiao-Han jiang Mu-Zi Li jian wang Qi-Qi chen Rui Lei cheng-cheng zhao Yun-Qiu zhou zheng-wang jiang Zuo-zhou Li Shang zheng Chang Dong Bai-Lin wang Yan-Xiang Sun Hui-Qin Zhang jie-Wei Li Quan-Hui Mo Ying Zhang Xin Lou Hai-Xu peng Ya-Ting Yi He-Xin wang Xiu-Jun Zhang Yi-Bo wang Dan wang Li Li Qiong Zhang Wen-Xia wang Yongbo Liu Lei Gao jin-Hu Wu Yan-Chang wang CAS key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture Wuhan Botanical GardenChinese Academy of SciencesWuhanHubeiChina key Laboratory of Western china’s Environmental Systems(Ministry of Education) College of Earth and Environmental SciencesLanzhou UniversityLanzhouGansuChina college of life {2. University of Chinese Academy of SciencesBeijingChina Wuhan Frasergen Bioinformatics Co. LtdWuhanHubeiChina Institute of Soil and Fertilizer Anhui Academy of Agricultural SciencesHefeiChina college of Agricultural sciences Xichang UniversityXichangSichuanChina Department of Horticulture Heilongjiang Academy of Agricultural SciencesHarbinHeilongjiangChina college of life {2. Langfang Normal UniversityLangfangHebeiChina Institute of Horticulture Zhejiang Academy of Agricultural SciencesHangzhouZhejiangChina Guangxi Institute of Botany Chinese Academy of SciencesGuilinGuangxiChina Xi’an Botanical Garden of Shaanxi Province Institute of Botany of Shaanxi ProvinceXi’anShaanxiChina Institute of Modern Agricultural Research Dalian UniversityDalianLiaoningChina Bioinformatics Center College of Plant Science and TechnologyBeijing University of AgricultureBeijingChina college of Agriculture Eastern Liaoning UniversityDandongLiaoningChina Dalian Institute of Chemical Physics Chinese Academy of SciencesDalianLiaoningChina state Environmental Protection key Laboratory of Regional Eco-process and Function Assessment Chinese Research Academy of Environmental SciencesBeijingChina Hubei Hongshan Laboratory WuhanHubeiChina The New Zealand Institute for Plant and Food Research Limited AucklandNew Zealand

Actinidia arguta,the most widely distributed Actinidia species and the second cultivated species in the genus,can be distinguished from the currently cultivated Actinidia chinensis on the basis of its small and smooth fruit,rapid softening,and excellent cold *** evolution of tetraploid Actinidia species and the genetic basis of their important agronomic traits are still ***,we generated a chromosome-scale genome assembly of an autotetraploid male *** *** genome assembly was 2.77 Gb in length with a contig N50 of 9.97 Mb and was anchored onto 116 *** and clustering of 101 geographically representative accessions showed that they could be divided into two geographic groups,Southern and Northern,which first diverged 12.9 million years *** underwent two prominent expansions and one demographic bottleneck from the midPleistocene climate transition to the late *** genomics studies using paleoclimate data enabled us to discern the evolution of the species’adaptation to different historical *** genes(AaCEL1,AaPME1,and AaDOF1)related to flesh softening were identified by multi-omics analysis,and their ability to accelerate flesh softening was verified through transient expression assays.A set of genes that characteristically regulate sexual dimorphism located on the sex chromosome(Chr3.or autosomal chromosomes showed biased expression during stamen or carpel *** chromosome-level assembly of the autotetraploid *** genome and the genes related to important agronomic traits will facilitate future functional genomics research and improvement of ***.

关键词： kiwiberry dioecism adaptive evolution paleoclimate GEA rapid softening

10B-doped MCP detector developed for neutron resonance imaging at Back-n white neutron source

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nuclear Science and Techniques 2024年第8期35卷 58-68页

作者： Qiang Li Li‑jiao wang jing‑Yu Tang Xiang‑Biao Qiu Zhen {3. Mao‑Yuan zhao Chang‑Jun Ning Kai Pan Wei Xu Tao Li Su‑peng Lu Han Yi Rui‑Rui Fan Chang‑Qing Feng Rong Zhang Xiao‑Yang Sun Qi An Hao‑Fan Bai jiang‑Bo Bai jie Bao Ping Cao Qi‑Ping {3. Yong‑Hao {3. Zeng‑Qi Cui An‑Chuan Fan Fan‑Zhen Feng Min‑Hao Gu Chang‑Cai Han Zi‑jie Han Guo‑Zhu He Yong‑{3.g He Yang Hong Yi‑Wei Hu Han‑Xiong Huang Wei jiang Zhi‑jie jiang zheng‑Yao jin Ling Kang Bo Li Gong Li Xiao Li Yang Li jie Liu Rong Liu Shu‑Bin Liu Yi‑Na Liu Guang‑Yuan Luan jie Ren Zhi‑zhou Ren Xi‑Chao Ruan zhao‑Hui Song Kang Sun Zhi‑Xin Tan Sheng‑Da Tang jin‑{3.g wang peng‑{3.g wang zhao‑Hui wang Zhong‑Wei Wen Xiao‑Guang Wu Xuan Wu Cong Xia Yong‑ji Yu Guo‑Hui Zhang Hang‑Chang Zhang Lin‑Hao Zhang Qi‑Wei Zhang Xian‑peng Zhang Yu‑Liang Zhang Yue Zhang Zhi‑Yong Zhang Zhi‑Hao zhou Ke‑Jun Zhu Chong Zou Institute of High Energy Physics Chinese Academy of Sciences(CAS)Beijing 100049China Spallation Neutron Source Science Center Dongguan 523803China state key Laboratory of Particle Detection and Electronics Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China Dongguan Songshan Lake Future School Dongguan 523808China School of Nuclear Science and Technology University of Science and Technology of ChinaHefei 230027China state key Laboratory of Particle Detection and Electronics University of Science and Technology of ChinaHefei 230026China North Night Vision Science and Technology(Nanjing)Research Institute Co.Ltd Nanjing 211106China Department of Modern Physics University of Science and Technology of ChinaHefei 230026China state key Laboratory of Nuclear Physics and Technology School of PhysicsPeking UniversityBeijing 100871China Institute of Modern Physics Fudan UniversityShanghai 200433China key Laboratory of Nuclear Data China Institute of Atomic EnergyBeijing 102413China china academy of Engineering Physics Institute of Nuclear Physics and ChemistryMianyang 621900China USTC archaeometry lab University of Science and Technology of ChinaHefei 230026China Northwest Institute of Nuclear Technology Xi’an 710024China university of chinese academy of sciences Beijing 100049China china Shipbuilding Trading Co. LtdBeijing 100044China

Neutron resonance imaging(NRI)has recently emerged as an appealing technique for neutron *** complexity surpasses that of conventional transmission imaging,as it requires a high demand for both a neutron source and ***,the progression of NRI technology has been sluggish since its inception in the 1980s,particularly considering the limited studies analyzing the neutron energy range above *** white neutron source(Back-n)at the china Spallation Neutron Source(CSNS)provides favorable beam conditions for the development of the NRI technique over a wide neutron energy range from eV to ***-sensitive microchannel plates(MCP)have emerged as a cutting-edge tool in the field of neutron detection owing to their high temporal and spatial resolutions,high detection efficiency,and low *** this study,we report the development of a 10B-doped MCP detector,along with its associated electronics,data processing system,and NRI experiments at the *** heavy elements such as gold,silver,tungsten,and indium can be easily identified in the transmission images by their characteristic resonance peaks in the 1–100 eV energy range;the more difficult medium-weight elements such as iron,copper,and aluminum with resonance peaks in the 1–100 keV energy range can also be *** particular,results in the neutron energy range of dozens of keV(Aluminum)are reported here for the first time.

关键词： Neutron resonance imaging 10B doped MCP detector White neutron source Sample nuclide identification

A draft sequence of the rice (Oryza sativa ssp. indica) genome

在线全文

学校读者我要写书评

暂无评论

chinese Science Bulletin 2001年第23期46卷 1937-1942页

作者： YU Jun, HU Songnian, wang Jun,LI Songgang WONG Ka-Shu Gane, LIU Bin,DENG Yajun, DAI Li, zhou Yan,ZHANG Xiuqing, CAO Mengliang, LIU jing,SUN jiandong , TANG jiabin, chen Yanjiong,HUANG Xiaobing, LIN Wei, YE chen, TONG Wei,CONG Lijuan, GENG jianing, HAN Yujun, LI Lin,LI Wei, HU Guangqiang, HUANG Xiangang,LI Wenjie, LI jian, LIU Zhanwei, LI Long,LIU jianping, Ql Qiuhui, LIU jinsong, LI Li,wang Xuegang, LU Hong, WU Tingling,ZHU Miao, Nl Peixiang, HAN Hua, DONG Wei,REN Xiaoyu, FENG Xiaoli, GUI peng,LI Xianran, wang Hao, XU Xin, ZHAI Wenxue,XU zhao, ZHANG jinsong, HE Sijie,ZHANG jianguo, XU jichen, ZHANG Kunlin,zheng Xianwu, DONG jianhai, ZENG Wanyong,TAO Lin, chen Xuewei, HE Jun, LIU Daofeng,TIAN Wei, TIAN Chaoguang, XIA Hongai,LI Gang, GAO Hui, LI Ping, chen Wei ,wang Xudong, ZHANG Yong, HU jianfei,wang jing, LIU Song, YANG jian,ZHANG Guangyu, XIONG Yuqing, LI Zhijie,MAO Long, zhou chengshu, ZHU Zhen,chen Runsheng, HAO Bailin,zheng Weimou, chen Shouyi, QUO Wei,LI Guojie, LIU Siqi, HUANG Guyang,TAO Ming, wang jian, ZHU Lihuang,YUAN Longping& YANG HuanmingBeijing Genomics Institute/Center of Genomics & Bioinformatics, chinese academy of sciences, Beijing 1013.0, china Hangzhou Genomics Institute/Institute of Bioinformatics of Zhejiang university/key Laboratory of Bioinformatics of Zhejiang Province, Hangzhou 3.0007, china Institute of Genetics, chinese academy of sciences, Beijing 100101, china National Hybrid Rice R & D Center, Changsha 410125, china Laboratory of Bioinformatics, Institute of biophysics, chinese academy of sciences, Beijing 100101, china college of life sciences, peking university, Beijing 100871, china Institute of Theoretical Physics, chinese academy of sciences, Beijing 1Q0080, china Digital china Ltd., Beijing 100080, china Institute of Computing Technology, chinese academy of sciences, Beijing 100080, china Medical college, Xi’an jiaotong university, Xi’an 710061, chinaThese authors contributed equally to this work.Corresponding author.Corresponden Beijing Genomics Institute/Center of Genomics & Bioinformatics Chinese Academy of Sciences Beijing China Hangzhou Genomics Institute/Institute of Bioinformatics of Zhe{3.ang university/key Laboratory of Bioinformatics of Zhe{3.ang Province Hangzhou China college of life {2. Peking University Beijing China Medical college Xi’an Jiaotong University Xi’an China Institute of Genetics Chinese Academy of Sciences Beijing China Laboratory of Bioinformatics Institute of Biophysics Chinese Academy of Sciences Beijing China National Hybrid Rice R & D Center Changsha China Institute of Theoretical Physics Chinese Academy of Sciences Beijing China Digital china Ltd. Beijing China Institute of Computing Technology Chinese Academy of Sciences Beijing China

The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development, and evolution, as well as information on many beneficial phenotypes of economic significance. Using a "whole genome shotgun" approach, we have pro-duced a draft rice genome sequence of Oryza sativa ssp. in-dica, the major crop rice subspecies in china and many other regions of Asia. The draft genome sequence is constructed from over 4.3.million successful sequencing traces with an accumulative total length of 2.14.9 Mb. The initial assembly of the non-redundant sequences reached 409.76 Mb in length, based on 3.3. million successful sequencing traces with a total length of 1797.4 Mb from an indica variant cultivar 93.11, giving an estimated coverage of 95.2.% of the rice genome with an average base accuracy of higher than 99%. The coverage of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEM-BLER, a custom-designed software package

关键词： rice genome draft sequences.