检索结果-南通市图书馆

Screening and identification of functional bacterial attachment genes in aerobic granular sludge

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2024年第7期141卷 205-214页

作者： Kai Qiao tingting zhao LeiWang Wei Zhang WeiMeng Fan Liu Xu Gao Jianrong Zhu School of Environment Beijing Normal UniversityBeijing 100875China State Key Laboratory of Water Simulation Beijing 100875China R&D Centre of Aerobic Granule Technology Beijing 100875China

The screening and identification of attachment genes is important to exploring the formation mechanism of biofilms at the gene *** is helpful to the development of key culture technologies for aerobic granular sludge(AGS).In this study,genome-wide sequencing and gene editing were employed for the first time to investigate the effects and functions of attachment genes in *** the help of whole-genome analysis,ten attachment genes were screened from thirteen genes,and the efficiency of gene screening was greatly ***,two attachment genes were selected as examples to further confirm the gene functions by constructing gene-knockout recombinant mutants of Stenotrophomonas maltophilia;when the two attachment genes were knocked out,the attachment potential was reduced by 50.67%and 43.93%,*** results provide a new theoretical principle and efficient method for the development of AGS from the perspective of attachment genes.

关键词： Attachment gene Genome-wide sequence Gene-knockout Attachment potential Aerobic granular sludge biofilm

Functional prediction of tomato PLATZ family members and functional verification of Sl PLATZ17

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2024年第1期23卷 141-154页

作者： Min Xu zhao Gao Dalong Li Chen Zhang Yuqi Zhang Qian He Yingbin Qi He Zhang Jingbin Jiang Xiangyang Xu tingting zhao College of Horticulture and Landscape Architecture Northeast Agricultural UniversityHarbin 150030China Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region) Ministry of Agriculture and Rural AffairsHarbin 150030China

PLATZ is a novel zinc finger DNA-binding protein that plays an important role in regulating plant growth and development and resisting abiotic ***,there has been very little research on the function of this family gene in tomatoes,which limits its application in germplasm resource ***,the PLATZ gene family was identified and analyzed in tomato,and its roles were predicted and verified to provide a basis for in-depth research on SlPLATZ gene *** this study,the PLATZ family members of tomato were identified in the whole genome,and 19 SlPLATZ genes were *** prediction was conducted based on gene and promoter structure analysis and RNA-seq-based expression pattern *** genes that responded significantly under different abiotic stresses or were significantly differentially expressed among multiple tissues were screened as functional gene ***17 was selected for functional verification by experiment-based *** results showed that the downregulation of SlPLATZ17 gene expression reduced the drought and salt tolerance of tomato *** plants overexpressing SlPLATZ17 had larger flower sizes and long,thin petals,adjacent petals were not connected at the base,and the stamen circumference was *** study contributes to understanding the functions of the SlPLATZ family in tomato and provides a reference for functional gene screening.

关键词： tomato PLATZ family abiotic stresses plant development

HDPairFinder:A data processing platform for hydrogen/deuterium isotopic labeling-based nontargeted analysis of trace-level amino-containing chemicals in environmental water

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2024年第2期136卷 583-593页

作者： tingting zhao Kristin Carroll Caley B.Craven Nicholas J.P.Wawryk Shipei Xing Jian Guo Xing-Fang Li Tao Huan Department of Chemistry Faculty of ScienceUniversity of British ColumbiaVancouver Campus2036 Main MallVancouverBritish ColumbiaV6T 1Z1Canada Division of Analytical and Environmental Toxicology Department of Laboratory Medicine and PathologyFaculty of Medicine and DentistryUniversity of AlbertaEdmontonAlberta T6G 2G3Canada

The combination of hydrogen/deuterium(H/D)formaldehyde-based isotopic methyl labeling with solid-phase extraction and high-performance liquid chromatography–high resolution mass spectrometry(HPLC-HRMS)is a powerful analytical solution for nontargeted analysis of trace-level amino-containing chemicals in water *** the huge amount of chemical information generated in HPLC-HRMS analysis,identifying all possible H/Dlabeled amino chemicals presents a significant challenge in data *** address this,we designed a streamlined data processing pipeline that can automatically extract H/D-labeled amino chemicals from the raw HPLC-HRMS data with high accuracy and ***,we developed a cross-correlation algorithm to correct the retention time shift resulting from deuterium isotopic effects,which enables reliable pairing of H-and D-labeled ***,we implemented several bioinformatic solutions to remove false chemical features generated by in-source fragmentation,salt adduction,and natural13C ***,we used a data mining strategy to construct the AMINES library that consists of over 38,000 structure-disjointed primary and secondary amines to facilitate putative compound ***,we integrated these modules into a freely available R program,*** rationale of each module was justified and its performance tested using experimental H/D-labeled chemical standards and authentic water *** further demonstrated the application of HDPairFinder to effectively extract N-containing contaminants,thus enabling the monitoring of changes of primary and secondary N-compounds in authentic water *** is a reliable bioinformatic tool for rapid processing of H/D isotopic methyl labeling-based nontargeted analysis of water samples,and will facilitate a better understanding of N-containing chemical compounds in water.

关键词： Isotope labeling Nontargeted analysis Reactive N-compounds HPLC-HRMS Source water

Human circBOULE RNAs as potential biomarkers for sperm quality and male infertility

在线全文

学校读者我要写书评

暂无评论

Journal of biomedical research 2024年第5期38卷 1-12页

作者： Liping Cheng He Jin Tianheng Xiao Xiaoyu Yang tingting zhao Eugene Yujun Xu State Key Laboratory of Reproductive Medicine and Offspring Health Nanjing Medical University Nanjing Jiangsu 211166 China. Center for Clinical Reproduction the First Affiliated Hospital with Nanjing Medical University & Jiangsu Province Hospital Nanjing Jiangsu 210029 China. Cellular Screening Center The University of Chicago Chicago IL 60637 USA.

Reliable molecular biomarkers to predict fertility remain scarce. The current study explored the potential of testis-specific RNAs as biomarkers for male infertility and sperm quality. Using RT-PCR and RT-qPCR assays, we identified seven circular RNAs from the human gene in human sperm. We found that sperm RNA exhibited a significantly decreased expression in asthenozoospermia while and expression decreased in teratozoospermia, compared with the controls. Furthermore, expression exhibited a negative correlation with sperm DNA Fragmentation Index (DFI), and levels were correlated with both fertilization and cleavage rates involving assisted reproductive technologies. Further functional analyses in a transgenic fly model lent support for the roles of RNAs in sperm development and human fertility. Collectively, our findings support that sperm RNAs may serve as diagnostic biomarkers for assessing sperm motility and DNA quality. Hence clinical application and significance of sperm circular RNAs in assisted reproductive technologies warrant further investigation.

关键词： assisted reproductive technology cleavage rate fertilization rate human circBOULE RNAs semen parameters sperm DNA fragment index

Human circBOULE RNAs as potential biomarkers for sperm quality and male infertility

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Journal of Biomedical Research 2024年第05期 473-484+397-402页

作者： Liping Cheng He Jin Tianheng Xiao Xiaoyu Yang tingting zhao Eugene Yujun Xu State Key Laboratory of Reproductive Medicine and Offspring Health Nanjing Medical University Center for Clinical Reproduction the First Affiliated Hospital with Nanjing Medical University & Jiangsu Province Hospital Cellular Screening Center the University of Chicago

Reliable molecular biomarkers to predict fertility remain scarce. The current study investigated the potential of testis-specific circBOULE RNAs as biomarkers for male infertility and sperm quality. Using reverse transcription-PCR and real-time reverse transcription-PCR assays, we identified seven circular RNAs from the human BOULE gene in human sperm. We observed that the expression level of circEx3-6 was significantly reduced in asthenozoospermia, while the expression levels of both circEx2-6 and circEx2-7 were decreased in teratozoospermia, compared with the controls. Furthermore, we demonstrated that the expression level of circEx2-6 was negatively correlated with the sperm DNA fragmentation index, and the expression level of circEx2-7 was correlated with both fertilization and cleavage rates in those treated with the assisted reproductive *** functional analyses in a transgenic fly model supported the roles of circBOULE RNAs in sperm development and human male fertility. Collectively, our findings support that sperm circBOULE RNAs may serve as diagnostic biomarkers for assessing sperm motility and DNA quality. Therefore, clinical application and significance of sperm circBOULE RNAs in the assisted reproductive technologies warrant further investigation.

关键词：

从另一个视角审视梅兰芳访美演出的艰辛历程与文化意义——以纽约公共图书馆馆藏16封信件为依据

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

戏剧艺术 2024年第4期 143-159页

作者：赵婷婷美国弗吉尼亚理工大学现代与古典语言及文学系

纽约公共图书馆馆藏的16封信件显示了梅兰芳访美演出所涉的大量准备工作。在这个过程中,中美双方诸多人士交流沟通,通力协作,准备工作中包括可行性论证、目标定位、市场和观众预测,以及经纪人、剧场、剧目等诸多事项选择与安排。上述所... 详细信息

纽约公共图书馆馆藏的16封信件显示了梅兰芳访美演出所涉的大量准备工作。在这个过程中,中美双方诸多人士交流沟通,通力协作,准备工作中包括可行性论证、目标定位、市场和观众预测,以及经纪人、剧场、剧目等诸多事项选择与安排。上述所有工作都经历了多方探索与反复磋商,而参与这一过程的美国人士涉及当时的社会各界,显示了人心所向和人际网络的作用。梅兰芳的访美演出取得了巨大成功,实现了文化交流的目标。文化交流是梅兰芳1930年访美演出贯彻始终的第一要义,不仅访问演出本身是文化交流,筹备访问演出的过程也是文化交流。

关键词：梅兰芳访美演出人际网络文化交流

维普期刊数据库博看期刊

对标高等教育强国:我国高等教育系统关键指标的国际对比分析

在线全文

学校读者我要写书评

暂无评论

国家教育行政学院学报 2024年第3期315卷 18-28页

作者：赵婷婷厦门大学福建厦门361005

对标世界高等教育强国,可以从发展水平和贡献能力两个方面来把握我国高等教育系统的发展状况。在发展水平方面,从体量规模、经费投入、师资队伍、国际化四个维度选择了十个关键指标进行国际对比分析;在贡献能力方面,从教育机会获得、满... 详细信息

对标世界高等教育强国,可以从发展水平和贡献能力两个方面来把握我国高等教育系统的发展状况。在发展水平方面,从体量规模、经费投入、师资队伍、国际化四个维度选择了十个关键指标进行国际对比分析;在贡献能力方面,从教育机会获得、满足社会发展需要、促进科技创新发展三个维度选择了七个关键指标进行国际对比分析。结果表明,改革开放以来,我国高等教育发展取得了一些成就,但是仍存在诸多问题,只有客观认识成就、准确研判问题,才能找到未来建设教育强国的发力点和突破点。

关键词：高等教育强国关键指标国际对比发展水平贡献能力

中国大学生高阶思维能力测试的本土开发与质量验证

在线全文

学校读者我要写书评

暂无评论

教育科学 2024年第1期40卷 78-86页

作者：赵婷婷杨翊厦门大学高等教育发展研究中心福建厦门361005 北京语言大学语言科学院北京100083

随着信息化、智能化社会的到来,大学生高阶思维能力的培养受到了前所未有的关注。本研究从中国实情出发,秉持“科学性”和“本土性”的原则,依据大学生学习成果评价和2001版布鲁姆教育目标分类学等理论,开发了中国大学生高阶思维能力测... 详细信息

随着信息化、智能化社会的到来,大学生高阶思维能力的培养受到了前所未有的关注。本研究从中国实情出发,秉持“科学性”和“本土性”的原则,依据大学生学习成果评价和2001版布鲁姆教育目标分类学等理论,开发了中国大学生高阶思维能力测试,阐述了这项测试的具体研发过程,并依据三所高校的实测数据,基于教育测量学标准化测试的质量分析方法,对这项测试的信度、效度、试题质量、等值方案的合理性进行了分析。结果表明,这项测试具有良好的信度,合理的结构效度,对我国不同类型高校的学生和不同年级的学生具有较好的区分能力。依据经典测验理论完成的试题参数分析表明,试题质量良好;测试采用非等组共同题设计完成不同版本试卷间的等值,等值方案科学。这些分析表明,中国大学生高阶思维能力测试作为一项严格意义上的标准化测试稳定、可靠、有效,适用于我国大学生学习成果评价实践,其研发过程和质量验证方法对同类测试具有重要的启发意义和参考价值。

关键词：高阶思维能力大学生学习成果测试质量中国等值

在线全文

学校读者我要写书评

暂无评论

儿童口呼吸相关错畸形的早期矫治

中华口腔医学杂志 2024年第9期59卷 892-897页

作者：赵婷婷贺红武汉大学口腔医(学)院口颌系统重建与再生全国重点实验室口腔生物医学教育部重点实验室口腔医学湖北省重点实验室武汉

口呼吸是儿童最常见的口颌面功能异常表现。儿童口呼吸常伴阻塞性睡眠呼吸暂停的发生, 其不仅影响儿童颅颌面发育、导致错畸形, 还可能引起一系列严重的全身并发症。口呼吸及其相关错畸形的诊断治疗需要多学科协作。本文从口呼吸的病因... 详细信息

口呼吸是儿童最常见的口颌面功能异常表现。儿童口呼吸常伴阻塞性睡眠呼吸暂停的发生, 其不仅影响儿童颅颌面发育、导致错畸形, 还可能引起一系列严重的全身并发症。口呼吸及其相关错畸形的诊断治疗需要多学科协作。本文从口呼吸的病因与危害、口呼吸相关问题的诊断、口呼吸与错畸形的相关性、口呼吸相关错畸形的治疗4个方面进行论述, 以期为临床提供参考。

关键词：错儿童口呼吸正畸学, 预防正畸学, 阻断生长和发育早期矫治