检索结果-南通市图书馆

孕期微生物组的时空模式及其与妊娠期综合征的联系

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science Bulletin 2024年第9期69卷 1275-1285页

作者：肖力文周田左振强孙宁霞赵方庆 Beijing Institutes of Life Science Chinese Academy of SciencesBeijing 100101China Department of Reproductive Medicine Second Afﬁliated Hospital of Naval Medical UniversityShanghai 200003China Key Laboratory of Systems Biology Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China University of Chinese Academy of Sciences Beijing 100049China

The microbiome of females undergoes extensive remodeling during pregnancy, which is likely to have an impact on the health of both mothers and offspring. Nevertheless, large-scale integrated investigations characterizing microbiome dynamics across key body habitats are lacking. Here, we performed an extensive meta-analysis that compiles and analyzes microbiome profiles from >10,000 samples across the gut,vagina, and oral cavity of pregnant women from diverse geographical regions. We have unveiled unexpected variations in the taxonomic, functional, and ecological characteristics of microbial communities throughout the course of pregnancy. The gut microbiota showed distinct trajectories between Western and non-Western populations. The vagina microbiota exhibited fluctuating transitions at the genus level across gestation, while the oral microbiota remained relatively stable. We also identified distinctive microbial signatures associated with prevalent pregnancy-related disorders, including opposite variations in the oral and gut microbiota of patients with gestational diabetes and disrupted microbial networks in preterm birth. This study establishes a comprehensive atlas of the pregnancy microbiome by integrating multidimensional datasets and offers foundational insights into the intricate interplay between microbes and host factors that underlie reproductive health.

关键词： Pregnancy microbiome Data integration Spatiotemporal patterns Microbial biomarkers

孕期微生物:解密母体微生态,为母婴健康保驾护航

在线全文

学校读者我要写书评

暂无评论

科学通报 2024年第17期69卷 2321-2323页

作者：肖力文周田左振强孙宁霞赵方庆中国科学院北京生命科学研究院北京100101 海军军医大学第二附属医院生殖医学中心上海200003 中国科学院大学杭州高等研究院系统生物学重点实验室杭州310024 中国科学院大学北京100049

怀孕是女性生命中的一段重要时光,是每位准妈妈生命中的一段特殊旅程,它不仅伴随着母亲身体的巨大变化,还涉及母亲微生物组在体内的复杂互动[1,2].微生物组是指生活在我们身体内外的微小生物的总体,包括细菌、真菌和病毒等.这些微生物... 详细信息

怀孕是女性生命中的一段重要时光,是每位准妈妈生命中的一段特殊旅程,它不仅伴随着母亲身体的巨大变化,还涉及母亲微生物组在体内的复杂互动[1,2].微生物组是指生活在我们身体内外的微小生物的总体,包括细菌、真菌和病毒等.这些微生物在我们的身体中扮演着关键的角色,影响着我们的健康和疾病的发生发展[3,4].孕期女性体内存在着庞大而复杂的微生物群落.这一群落包括了肠道、阴道和口腔等多个部位的微生物,它们与孕妇的健康和胎儿的发育息息相关[5,6].

关键词：微生物组女性生命微生物群落母婴健康微生态准妈妈

Single-cell omics:experimental workflow,data analyses and applications

在线全文

学校读者我要写书评

暂无评论

Science China Life Sciences 2024年

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu Fu Lei Gu Xin Shao Qinqin Wang Xin Dong Bin Duan Feiyang Xing Jun Wu Minmin Xiao fangqing zhao Jing-Dong J.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biology the Institute of Biomedical Sciences and School of Life Sciences East China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary Studies Center for Quantitative Biology (CQB) Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women’s Hospital Zhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of Statistics East China Normal University Shanghai 200062 China Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical Sciences Zhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of Education Orthopaedic Department Tongji Hospital Bioinformatics Department School of Life Sciences and Technology Tongji University Frontier Science Center for Stem Cells School of Life Sciences and Technology Tongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East Hospital Bioinformatics Department School of Life Sciences and Technology Tongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public Health Shanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River Delta Zhejiang University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial scRNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词：

Screening Linear and Circular RNA Transcripts from Stress Granules

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2023年第4期21卷 886-893页

作者： Shuai Chen Jinyang Zhang fangqing zhao Beijing Institutes of Life Science Chinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunming 650223China Key Laboratory of Systems Biology Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310013China

Stress granules(SGs)are cytoplasmic ribonucleoprotein assemblies formed under stress conditions and are related to various biological processes and human diseases.Previous studies have reported the regulatory role of some proteins and linear RNAs in SG assembly.However,the relationship between circular RNAs(circRNAs)and SGs has not been discovered.Here,we screened both linear RNAs and circRNAs in SGs using improved total RNA sequencing of purified SG cores in mammalian cells and identified circular transcripts specifically localized in SGs.circRNAs with higher SG-related RNA-binding protein(RBP)binding abilities are more likely to be enriched in SGs.Furthermore,some SG-enriched circRNAs are differentially expressed in hepatocellular carcinoma(HCC)and adjacent tissues.These results suggest the regulatory role of circRNAs in SG formation and provide insights into the biological function of circRNAs and SGs in HCC.

关键词： Stress granule Circular RNA Protein–RNA interaction RNA-seq Hepatocellular carcinoma

Dysregulation of circular RNAs in inflammation and cancers

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Fundamental Research 2023年第5期3卷 683-691页

作者： Jiayu Liu fangqing zhao Ling-Ling Chen Shicheng Su Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation Medical Research CenterSun Yat-Sen Memorial HospitalSun Yat-Sen UniversityGuangzhou 510120China Breast Tumor Center Sun Yat-Sen Memorial HospitalSun Yat-Sen UniversityGuangzhou 510120China Beijing Institutes of Life Science Chinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunming 650223China State Key Laboratory of Molecular Biology Shanghai Key Laboratory of Molecular AndrologyCAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesShanghai 200031China School of Life Science and Technology ShanghaiTech UniversityShanghai 200092China School of Life Science Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesChinese Academy of SciencesHangzhou 310003China Department of Immunology Zhongshan School of MedicineSun Yat-Sen UniversityGuangzhou 510080China

Emerging lines of evidence have shown that the production of the covalently closed single-stranded circular RNAs is not splicing errors,but rather a regulated process with distinct biogenesis and turnover.Circular RNAs are expressed in a cell type-and tissue-specific manner and often localize to specific subcellular regions or organelles for functions.The dysregulation of circular RNAs from birth to death is linked to the pathogenesis and progression of diverse diseases.This review outlines how aberrant circular RNA biogenesis,subcellular location,and degradation are linked to disease progression,focusing on metaflammation and cancers.We also discuss potential therapeutic strategies and obstacles in targeting such disease-related circular RNAs.

关键词： Circular RNA Metaflammation Biogenesis Degradation Subcellular localization

First Glimpse of Gut Microbiota of Quarantine Insects in China

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2022年第2期20卷 394-404页

作者： Yanxue Yu Qi Wang Ping Zhou Na Lv Wei Li fangqing zhao Shuifang Zhu Di Liu Institute of Plant Quarantine Chinese Academy of Inspection and QuarantineBeijing 100176China Institute of Physical Science and Information Technology Anhui UniversityHefei 230601China Computational Virology Group Center for Bacteria and Virus Resources and BioinformationWuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Computational Genomics Lab Beijing Institutes of Life ScienceChinese Academy of SciencesBeijing 102206China University of Chinese Academy of Sciences Beijing 100049China

Quarantine insects are economically important pests that frequently invade new habitats.A rapid and accurate monitoring method to trace the geographical sources of invaders is required for their prevention,detection,and eradication.Current methods based on genetics are typically time-consuming.Here,we developed a novel tracing method based on insect gut microbiota.The source location of the insect gut microbiota can be used to rapidly determine the geographical origin of the insect.We analyzed 179 gut microbiota samples from 591 individuals of 22 quarantine insect species collected from 36 regions in China.The gut microbiota of these insects primarily included Actinobacteria,Bacteroidetes,Cyanobacteria,Firmicutes,Proteobacteria,and Tenericutes.The diversity of the insect gut microbiota was closely associated with geographical and environmental factors.Different insect species could be distinguished based on the composition of gut microbiota at the phylum level.Populations of individual insect species from different regions could be distinguished based on the composition of gut microbiota at the phylum,class,and order levels.A method for determining the geographical origins of invasive insect species has been established;however,its practical application requires further investigations before implementation.

关键词： Quarantine insect Geographical origin 16S rRNA Gut microbiota Genus level

Microbiota-gut-brain axis in autism spectrum disorder

在线全文

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2021年第9期48卷 755-762页

作者： You Yu fangqing zhao Beijing Institutes of Life Science Chinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Extensive studies, largely during the past decade, identify the dynamic and bidirectional interaction between the bacteria resident in the intestines and their host brain along the "microbiota-gut-brain axis". This interaction modulates the development and function of the central nervous system and is implicated in neurological disorders. As a neurodevelopmental disorder, autism spectrum disorder (ASD) is considered a historically defect in the brain. With accumulating evidence showing how the microorganisms modulate neural activities, more and more research is focusing on the role of the gut microbiota in mitigating ASD symptoms and the underlying mechanisms. In this review, we describe the intricate and crucial pathways via which the gut microbiota communicates with the brain, the microbiota-gut-brain axis, and summarize the specific pathways that mediate the crosstalk of the gut microbiota to the brain in ASD.

关键词： Microbiota-gut-brain axis in autism spectrum disorder ASD

Variation of the Vaginal Microbiome During and After Pregnancy in Chinese Women

在线全文

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2022年第2期20卷 322-333页

作者： Xiaoai Zhang Qingzhi Zhai Jinfeng Wang Xiuling Ma Bo Xing Hang Fan Zhiying Gao fangqing zhao Wei Liu State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijing 100071China Department of Obstetrics and Gynecology First Medical CenterThe General Hospital of the People’s Liberation ArmyBeijing 100853China Computational Genomics Lab Beijing Institutes of Life ScienceChinese Academy of SciencesBeijing 100101China Beijing Key Laboratory of Vector Borne and Natural Focus Infectious Disease Beijing 100071China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunming 650223China Key Laboratory of Systems Biology Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesChinese Academy of SciencesHangzhou 310024China University of Chinese Academy of Sciences Beijing 100049China

A comprehensive profiling of the vaginal microbial communities and their variability enables an accurate description of the microbiome in women.However,there is a lack of studies available on Chinese women.In the present study,the composition of the vaginal microbiota during pregnancy and the 6-week postpartum period of 454 Chinese women was characterized by sequencing the V3-V4 region of the 16S ribosomal RNA(rRNA)gene.The vaginal microbiome showed variations during pregnancy and the postpartum period based on the abortion history,hypertensive disorders,delivery mode,and maternal age.Co-variation of 22 bacterial taxa,including the Lactobacillus genus and two of its species,may account for the common characteristics of the vaginal microbiome under scenarios of different medical histories and pregnancy outcomes.In contrast,discriminant bacterial species were significantly different between women who had preterm birth(PTB)with and without premature rupture of membranes(PROM),and the community state type(CST)IV-A without any predominant Lactobacillus species in the microbiota was more prevalent during pregnancy in the PROM-PTB cases,suggesting that specific bacterial species could be considered to distinguish between different types of PTB.By providing data on Chinese women,this study will enrich the knowledge of the human microbiome and contribute to a better understanding of the association between the vaginal microbiome and reproductive health.

关键词： Chinese pregnant women Vaginal microbiota Postpartum Pregnancy complication Premature rupture of membranes Preterm birth

Single-cell metagenomics： challenges and applications

在线全文

学校读者我要写书评

暂无评论

Protein & Cell 2018年第5期9卷 501-510页

作者： Yuan Xu fangqing zhao Computational Genomics Lab Beijing Institutes of Life Science Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China

With the development of high throughput sequencing and single-cell genomics technologies, many uncultured bacterial communities have been dissected by combining these two techniques. Especially, by simul- taneously leveraging of single-cell genomics and metagenomics, researchers can greatly improve the efficiency and accuracy of obtaining whole genome information from complex microbial communities, which not only allow us to identify microbes but also link function to species, identify subspecies variations, study host-virus interactions and etc. Here, we review recent developments and the challenges need to be addressed in single-cell metagenomics, including potential contamination, uneven sequence coverage, sequence chimera, genome assembly and annotation. With the development of sequencing and computational methods, single-cell metagenomics will undoubtedly broaden its application in various microbiome studies.

关键词： metagenomics bioinformatics single-celgenomics