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Sequentially releasing self-healing hydrogel fabricated with TGFβ3-microspheres and bFGF to facilitate rat alveolar bone defect repair

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Asian Journal of Pharmaceutical Sciences 2022年第3期17卷 425-434页

作者： Fenglin Yu Dezhi Geng zhanpeng kuang Shiyi Huang Yating Cheng Yini Chen Fang Leng Yu Bei Yueping Zhao Qingxia Tang Yadong Huang Qi Xiang Department of Cell Biology College of Life Science and TechnologyJinan UniversityGuangzhou 510632China Guangdong Province Key Laboratory of Bioengineering Medicine Jinan UniversityGuangzhou 510632China Biopharmaceutical R&D Center of Jinan University Guangzhou 510632China Department of Stomatology Jinan University Medical CollegeGuangzhou 510632China

Resorption and loss of alveolar bone leads to oral dysfunction and loss of natural or implant teeth. Biomimetic delivery of growth factors based on stem cell recruitment and osteogenic differentiation, as the key steps in natural alveolar bone regenerative process, has been an area of intense research in recent years. A mesoporous self-healing hydrogel(DFH) with basic fibroblast growth factor(bFGF) entrapment and transforming growth factor β3(TGFβ3)-loaded chitosan microspheres(CMs) was developed. The formulation was optimized by multiple tests of self-healing, in-bottle inversion, SEM, rheological, swelling rate and in vitro degradation. In vitro tubule formation assays, cell migration assays, and osteogenic differentiation assays confirmed the ability of DFH to promote blood vessels, recruit stem cells, and promote osteogenic differentiation. The optimum DFH formula is 0.05 ml 4ArmPEG-DF(20%) added to 1 ml CsGlu(2%) containing bFGF(80 ng) and TGFβ3-microspheres(5 mg). The results of in vitro release studied by Elisa kit, indicated an 95% release of b FGF in7 d and long-term sustained release of TGFβ3. For alveolar defects rat models, the expression levels of CD29 and CD45, the bone volume fraction, trabecular number, and trabecular thickness of new bone monitored by Micro-CT in DFH treatment groups were significantly higher than others(*P < 0.05, vs Model). HE and Masson staining show the same *** conclusion, DFH is a design of bionic alveolar remodelling microenvironment, that is in early time microvessels formed by b FGF provide nutritious to recruited endogenous stem cells, then TGFβ3 slowly released speed up the process of new bones formation to common facilitate rat alveolar defect repair. The DFH with higher regenerative efficiency dovetails nicely with great demand due to the requirement of complicated biological processes.

关键词： Self-healing hydrogel TGFβ3 microspheres bFGF Sequential release Alveolar defects

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猪流行性腹泻病毒在规模化抗体阳性猪场的动态传播

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微生物学通报 2024年第9期51卷 3572-3580页

作者：亢麒翔胡广利耿瑞王暄慈邝展鹏黄逸晖曾思莹彭欧阳张灏曹永长中山大学生命科学学院广东广州519000

【背景】猪流行性腹泻病毒(porcine epidemic diarrhea virus,PEDV)是导致仔猪腹泻的最主要病原之一。因其发病迅速难以记录,目前尚无关于PEDV感染仔猪后病原在猪场内分布的动态研究报道。【目的】探索PEDV在规模化猪场内的动态传播过程... 详细信息

【背景】猪流行性腹泻病毒(porcine epidemic diarrhea virus,PEDV)是导致仔猪腹泻的最主要病原之一。因其发病迅速难以记录,目前尚无关于PEDV感染仔猪后病原在猪场内分布的动态研究报道。【目的】探索PEDV在规模化猪场内的动态传播过程,改进防控猪流行性腹泻(porcine epidemic diarrhea,PED)的策略。【方法】模拟PEDV在封闭的规模化养殖场中感染仔猪后的传播过程,检测猪群及场内不同区域的病毒丰度。【结果】仔猪肛拭子和空气样品病毒丰度在仔猪感染PEDV初期具有明显的一致性变化,PEDV在场内的传播与员工移动密切相关,并且洗澡间可能是PEDV的重要藏匿点。【结论】猪舍空气的病毒丰度有可能作为PED早期的监测指标,并且员工相关的介质和场地是PEDV生物安全防控的重要对象。本研究为改进猪场针对PED的生物安全防控提供了一定参考,并且为类似病毒在规模化猪场传播的研究工作奠定了基础。

关键词：猪流行性腹泻病毒外环境生物安全防控

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Simultaneous determination of 35 constituents and elucidation of effective constituents in a multi-herb Chinese medicine formula Xiaoer-Feire-Kechuan

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Journal of Pharmaceutical Analysis 2021年第6期11卷 717-725页

作者： zhanpeng Shang Lulu Xu Yi kuang Yan Lin Shuang Liu Long Sun Tao Bo Min Ye Xue Qiao State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking UniversityBeijing 100191China School of Pharmaceutical Sciences Guizhou Medical UniversityGuizhou550025China Thermo Fisher Scientific Beijing100102China

Xiaoer-Feire-Kechuan(XFK) is an 11-herb Chinese medicine formula to treat cough and pulmonary *** complicated composition rendered its chemical analysis and effective-component *** this study,we combined quantitative analysis and bioactivity test to reveal the antiinflammatory constituents of ***,UPLC-DAD and UHPLC/Q-Orbitrap-MS methods were established and validated to quantify 35 analytes(covering 9 out of 11 herbs) in different XFK *** reaction monitoring mode built in Q-Orbitrap-MS was used to improve the sensitivity and ***,anti-inflammatory activities of the 35 analytes were analyzed using in vitro COX-2 inhibition ***,major analytes forsythosides H,I,A(8-10),and baicalin(15)(total contents varied from 21.79 to 91.20 mg/dose in different formulations) with significant activities(inhibitory rate ≥ 80%) were proposed as the anti-inflammatory constituents of *** present study provided an effective strategy to discover effective constituents of multi-herb formulas.

关键词： Multi-herb formulas Effective constituents Xiaoer-Feire-Kechuan formula(XFK) Parallel reaction monitoring(PRM) Cyclooxygenase-2(COX-2)

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