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Development of an Agrobacterium-mediated CRISPR/Cas9 gene editing system in jute(Corchorus capsularis)

The Crop Journal 2024年第4期12卷 1266-1270页

作者： Shaolian Jiang Qin Li Xiangxue Meng Mengxin huang Jiayu Yao Chuanyu wang Pingping Fang Aifen Tao Jiantang Xu Jianmin Qi Shuangxia Jin Liwu zhang Key Laboratory of Ministry of Education for Genetic Breeding and Multiple Utilization of Crops Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Experiment Station of Ministry of Agriculture and Rural Affairs for Jute and Kenaf in Southeast China/Fujian Public Platform for Germplasm Resources of Bast Fiber Crops/Fujian Provincial Key Laboratory of Crop Breeding by Design/Key Laboratory of Ministry of Agriculture and Rural Affairs for Biological Breeding of Fujian and Taiwan Crops Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina National Key Lab of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina

Jute(Corchorus capsularis L.)is the second most important natural plant fiber source after ***,developing an efficient gene editing system for jute remains a *** this study,the transgenic hairy root system mediated by Agrobacterium rhizogenes strain K599 was developed for Meifeng 4,an elite jute variety widely cultivated in *** transgenic hairy root system for jute was verified by subcellular localization and bimolecular fluorescence complementation(BiFC)*** CHLOROPLASTOS ALTERADOS 1(CcCLA1)gene,which is involved in the development of chloroplasts,was targeted for editing at two sites in Meifeng *** on this hairy root transformation,the gRNA scaffold was placed under the control of cotton ubiquitin GhU6.7 and-GhU6.9 promoters,respectively,to assess the efficiency of gene *** indicated the 50.0%(GhU6.7)and 38.5%(GhU6.9)editing events in the target 2 alleles(gRNA2),but no mutation was detected in the target 1 allele(gRNA1)in transgenic-positive hairy ***1 gene editing at gRNA2 under the control of GhU6.7 in Meifeng 4 was also carried out by Agrobacterium tumefaciens-mediated *** CcCLA1 mutants were albinic,with a gene editing efficiency of 5.3%.These findings confirm that the CRISPR/Cas9 system,incorporating promoter GhU6.7,can be used as a gene editing tool for jute.

关键词： Jute Agrobacterium-mediated transformation Genome editing Hairy root system

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Versatile plant genome engineering using anti-CRISPR-Cas12a systems

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Science China Life Sciences 2024年

作者： Yao He Shishi Liu Long Chen Dongkai Pu Zhaohui Zhong Tang Xu Qiurong Ren Chuan Dong Yawei wang Danning wang Xuelian Zheng Fengbiao Guo Tao zhang Yiping Qi Yong zhang Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City Chongqing Key Laboratory of Tree Germplasm Innovation and Utilization School of Life Sciences Southwest University Department of Biotechnology School of Life Sciences and Technology Center for Informational Biology University of Electronic Science and Technology of China Sichuan Institute of Edible Fungi Sichuan Academy of Agricultural Sciences Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of the Ministry of Education College of AgricultureYangzhou University Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Jiangsu Key Laboratory of Crop Genetics and Physiology Yangzhou University Department of Respiratory and Critical Care Medicine Zhongnan Hospital of Wuhan University Key Laboratory of Combinatorial Biosynthesis and Drug Discovery Ministry of Education and School of Pharmaceutical Sciences Wuhan University Department of Plant Science and Landscape Architecture University of Maryland College Park 20742 Institute for Bioscience and Biotechnology Research University of Maryland

CRISPR-Cas12a genome engineering systems have been widely used in plant research and crop breeding. To date, the performance and use of anti-CRISPR-Cas12a systems have not been fully established in plants. Here, we conduct in silico analysis to identify putative anti-CRISPR systems for Cas12a. These putative anti-CRISPR proteins, along with known anti-CRISPR proteins, are assessed for their ability to inhibit Cas12a cleavage activity in vivo and in planta. Among all anti-CRISPR proteins tested, AcrVA1 shows robust inhibition of Mb2Cas12a and LbCas12a in E. coli. Further tests show that AcrVA1 inhibits LbCas12a mediated genome editing in rice protoplasts and stable transgenic lines. Impressively, co-expression of AcrVA1 mitigates off-target effects by CRISPR-LbCas12a, as revealed by whole genome sequencing. In addition, transgenic plants expressing AcrVA1 exhibit different levels of inhibition to LbCas12a mediated genome editing, representing a novel way of fine-tuning genome editing efficiency. By controlling temporal and spatial expression of AcrVA1, we show that inducible and tissue specific genome editing can be achieved in plants. Furthermore, we demonstrate that AcrVA1 also inhibits LbCas12a-based CRISPR activation(CRISPRa) and based on this principle we build logic gates to turn on and off target genes in plant cells. Together, we have established an efficient anti-CRISPR-Cas12a system in plants and demonstrate its versatile applications in mitigating off-target effects, finetuning genome editing efficiency, achieving spatial-temporal control of genome editing, and generating synthetic logic gates for controlling target gene expression in plant cells.

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基于变载波频率的永磁同步电机驱动系统效率优化控制

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东南大学学报（自然科学版） 2024年第3期54卷 724-729页

作者：刘凯张阔花为胡铭觐东南大学电气工程学院南京210096

为降低永磁同步电机驱动系统的损耗,提高系统效率,提出了一种基于母线电流反馈的变载波频率优化控制方法.首先,建立了系统损耗数学模型,探究了载波频率与系统损耗的关系.然后,针对离线优化方法对于参数敏感的问题,设计了一种基于母线电... 详细信息

为降低永磁同步电机驱动系统的损耗,提高系统效率,提出了一种基于母线电流反馈的变载波频率优化控制方法.首先,建立了系统损耗数学模型,探究了载波频率与系统损耗的关系.然后,针对离线优化方法对于参数敏感的问题,设计了一种基于母线电流反馈的载波频率在线寻优方法.结果表明,在不同转速与负载条件下均存在使系统损耗最小的最优载波频率,其随转速与负载条件变化而变化.在各个工作点,系统损耗的计算值均与实测值基本吻合.在线寻优方法在中高负载情况下能够实现约0.6%的效率优化,并且在负载变化的情况下仍然能够实现损耗优化.

关键词：变载波频率损耗模型效率优化永磁同步电机

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Sleep disturbances and psychomotor retardation in the prediction of cognitive impairments in patients with major depressive disorder

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World Journal of Psychiatry 2024年第10期14卷 1474-1483页

作者： Mi wang Wen-Tao Chen Hao-Ting wang Bang-Shan Liu Yu-Meng Ju Qiang-Li Dong Xiao-Wen Lu Jin-Rong Sun Liang zhang hua Guo Fu-Tao Zhao Wei-Hui Li Li zhang Ze-Xuan Li Mei Liao Yan zhang Jin Liu Ling-Jiang Li Department of Mental Health Center Xiangya HospitalCentral South UniversityChangsha 410008Hunan ProvinceChina National Clinical Research Center of Geriatric Disorders Xiangya HospitalCentral South UniversityChangsha 410008Hunan ProvinceChina Department of Psychiatry National Clinical Research Center for Mental DisordersThe Second Xiangya Hospital of Central South UniversityChangsha 410011Hunan ProvinceChina Department of Psychiatry Zhumadian Psychiatric HospitalZhumadian 463000Henan ProvinceChina

BACKGROUND Symptoms of depression and comorbid anxiety are known risk factors for cognitive impairment in major depressive disorder(MDD).Understanding their relationships is crucial for developing targeted interventions to mitigate cognitive impairments in MDD *** expect that the severity of sleep disturbances and other depressive symptoms will be positively correlated with the degree of cognitive *** also hypothesize that anxiety symptoms,especially psychic anxiety,is a key factor in predicting cognitive performance in MDD patients and may indirectly contribute to cognitive impairment by affecting sleep disturbances and other potential *** To determine which dimension of the depressive and anxiety symptoms predicts cognitive impairment during a depressive *** A comprehensive neurocognitive test battery assessed executive function,attention,processing speed,and memory in 162 medication-free MDD patients and 142 matched healthy *** 24-item Hamilton Depression Rating Scale was used to assess depressive symptoms,and the 14-item Hamilton Anxiety Scale was used to assess anxiety *** regression analyses and mediation analyses were conducted to evaluate the impact of depressive and anxiety symptoms,as well as their interactions,on cognitive *** Among the depressive symptoms,sleep disturbances were associated with poorer executive function(P=0.004),lower processing speed(P=0.047),and memory impairments(P<0.001),and psychomotor retardation(PR)was associated with lower processing speed in patients with MDD(P=0.019).Notably,PR was found to mediate the impact of sleep disturbances on the processing *** anxiety symptoms,psychic anxiety,rather than somatic anxiety,was associated with cognitive impairments in all *** disturbances mediated the effect of psychic anxiety on executive function[β=-0.013,BC CI(-0.027,-0.001)]and memory[β=-0.149,BC CI(-0.237,-0.063)],while PR mediated its ef

关键词： Major depressive disorder Cognitive impairment Depressive symptoms Anxiety symptoms Sleep disturbance Psychomotor retardation

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ITER氦冷固态实验包层模块第一壁氢同位素双向输运数值分析

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核化学与放射化学 2024年第2期46卷 125-130,I0004页

作者：王俊张龙王晓宇武兴华李茹烟核工业西南物理研究院四川成都610225

氦冷固态实验包层模块(HCCB-TBM)安装于国际热核聚变实验堆(ITER)中,用以验证HCCB包层概念的氚增殖能力与热移出能力。HCCB-TBM第一壁用于承受堆芯等离子体粒子轰击和包容内部功能材料。外侧等离子体驱动氘、氚粒子渗透与内侧氢分压驱... 详细信息

氦冷固态实验包层模块(HCCB-TBM)安装于国际热核聚变实验堆(ITER)中,用以验证HCCB包层概念的氚增殖能力与热移出能力。HCCB-TBM第一壁用于承受堆芯等离子体粒子轰击和包容内部功能材料。外侧等离子体驱动氘、氚粒子渗透与内侧氢分压驱动渗透的同时存在,形成了第一壁的双向氢同位素输运。此双向输运可能对第一壁外表面再循环系数、包层增殖氚的纯化产生重要影响。基于商业软件COMSOL建立第一壁双向氢同位素输运模型,研究第一壁的氢同位素的输运特征。仿真结果表明:第一壁中的冷却剂流道具有强的氢同位素移出能力,使得双向输运解耦合;在ITER等离子体脉冲周期中,放电过程中已扩散到材料内部的氚在等离子体关停时扩散回流到真空室侧,关停时的回流将降低向冷却剂流道的氚渗透损失。

关键词： HCCB-TBM 第一壁氢同位素双向输运

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Potentiation of the lateral habenula-ventral tegmental area pathway underlines the susceptibility to depression in mice with chronic pain

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Science China(Life Sciences) 2024年第1期67卷 67-82页

作者： Chun-Kui zhang Pan wang Yuan-Yuan Ji Jian-Shuai Zhao Jun-Xiang Gu Xian-Xia Yan Hong-Wei Fan Ming-Ming zhang Yu Qiao Xiao-Die Liu Bao-Juan Li Ming-Hui wang Hai-Long Dong Hao-Hong Li Peng-Cheng huang Yun-Qing Li Wu-Gang Hou Jin-Lian Li Tao Chen Department of Anatomy and K.K.Leung Brain Research Centre Fourth Military Medical UniversityXi’an 710032China Beijing Institute of Basic Medical Sciences Beijing 100850China Key Laboratory of Ministry of Public Health for Forensic Science Xi’an Jiaotong UniversityXi’an 710061China Department of Anesthesiology Xijing HospitalFourth Military Medical UniversityXi’an 710032China Department of Neurosurgery the Second Affiliated Hospital of Xian Jiaotong UniversityXi’an 710004China School of Biomedical Engineering Fourth Military Medical UniversityXi’an 710032China Affiliated Mental Health Centre and Hangzhou Seventh People’s Hospital Zhejiang University School of MedicineHangzhou 310013China The MOE Frontier Research Center of Brain&Brain-machine Integration Zhejiang University School of Brain Science and Brain MedicineHangzhou 310058China Department of Neurology The First Affiliated Hospital of Nanchang UniversityNanchang 330006China Department of Anatomy School of MedicineNorthwest UniversityXi’an 710069China

Chronic pain often develops severe mood changes such as ***,how chronic pain leads to depression remains elusive and the mechanisms determining individuals’responses to depression are largely *** we found that depression-like behaviors could only be observed in 67.9%of mice with chronic neuropathic pain,leaving 32.1%of mice with depression *** determined that the spike discharges of the ventral tegmental area(VTA)-projecting lateral habenula(LHb)glutamatergic(Glu)neurons were sequentially increased in sham,resilient and susceptible mice,which consequently inhibited VTA dopaminergic(DA)neurons through a LHbGlu-VTAGABA-VTADA ***,the LHbGlu-VTADA excitatory inputs were dampened via GABAB receptors in a pre-synaptic *** of LHb-VTA pathway largely affected the development of depressive symptoms caused by chronic *** study thus identifies a pivotal role of the LHb-VTA pathway in coupling chronic pain with depression and highlights the activity-dependent contribution of LHbGlu-to-VTADA inhibition in depressive behavioral regulation.

关键词： chronic pain depression lateral habenula ventral tegmental area dopamine susceptibility

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Probing multi-physical process and deformation mechanism of a largescale landslide using integrated dual-source monitoring

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Geoscience Frontiers 2024年第2期15卷 417-432页

作者： Hong-Hu Zhu Xiao Ye hua-Fu Pei Wei zhang Gang Cheng Zi-Li Li School of Earth Sciences and Engineering Nanjing UniversityNanjing 210023China Department of Geotechnical Engineering Dalian University of TechnologyDalian 116024China Civil Structural&Environmental EngineeringSchool of Engineering and ArchitectureUniversity College CorkCorkIreland Department of Civil&Environmental Engineering Massachusetts Institute of TechnologyCambridgeMA 02139-4307United States

The implementation of isolated heterologous monitoring systems for spatially distant borehole deployments often comes with substantial equipment costs,which can limit the effectiveness of geohazard mitigation and georisk management *** address this,we have developed a novel monitoring system that integrates fiber Bragg grating(FBG)and microelectromechanical system(MEMS)techniques to capture soil moisture,temperature,sliding resistance,strain,surface tilt,and deep-seated *** system enables real-time,simultaneous data acquisition and cross-validation analyses,offering a costeffective solution for monitoring critical parameters in geohazard-prone *** successfully applied this integrated monitoring system to the Xinpu landslide,an active super-large landslide located in the Three Gorges Reservoir Area(TGRA)of *** resulting strain profile confirmed the presence of two shallow secondary sliding surfaces at depths of approximately 7 m and 12 m,respectively,in addition to the deep-seated sliding surface at a depth of28 *** lower secondary sliding surface was activated by extreme precipitation,while the upper one was primarily driven by significant changes in reservoir water levels and secondarily triggered by concentrated ***-slide piles have remarkably reinforced the upper moving masses but failed to control the lower *** gap between the pile heads and the soil amplified the rainwater erosion effect,creating a preferential channel for rainwater ***-physical measurements revealed a mixture of seepage-driven and buoyancy-driven behaviors within the *** study offers an integrated dual-source multi-physical monitoring paradigm that enables collaborative management of multiple crucial boreholes on a large-scale landslide,and contributes to the evaluation and improvement of engineering measures in similar geological settings.

关键词： Reservoir landslide Multi-physical process Integrated dual-source monitoring Fiber optic Extreme weather

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Two stages power generation test of the hot dry rock exploration and production demonstration project in the Gonghe Basin,northeastern Qinghai-Tibet plateau,China

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China Geology 2024年第3期7卷 409-421页

作者： Er-yong zhang Dong-guang Wen Gui-ling wang Xian-peng Jin Lin-you zhang Hai-dong Wu Wen-shi wang Cheng-ming Ye Wei Weng Kuan Li Jin-sheng Wu Xian-chun Tang Chong-yuan zhang Qing-da Feng Sheng Lian Li-sha Hu Gui-lin Zhu Xing-long Xie Bin Wu Dan wang Xue Niu Zhao-xuan Niu Dong-lin Liu Hui zhang Wen-hao Xu Shu-qing Yao Li Yang Center for Hydrogeology and Environmental Geology Survey China Geological SurveyMinistry of Natural ResourcesTianjin 300304China Institute of Hydrogeology and Environmental Geology Chinese Academy of Geological SciencesChina Geological SurveyShijiazhuang 050061China Institute of Exploration Techniques Chinese Academy of Geological SciencesChina Geological SurveyLangfang 065000China Institute of Exploration Engineering China Geological SurveyMinistry of Natural ResourcesBeijing 100083China Institute of Exploration Technology Chinese Academy of Geological SciencesChengdu 611734China Chinese Academy of Geological Sciences China Geological SurveyBeijing 100037China Institute of Geomechanics Chinese Academy of Geological SciencesChina Geological SurveyBeijing 100081China Geoscience Documentation Center China Geological SurveyMinistry of Natural ResourcesBeijing 100083China

The Hot Dry Rock(HDR)is considered as a clean and renewable energy,poised to significantly contribute to the global energy decarbonization *** HDR projects worldwide have accumulated valuable experience in efficient drilling and completion,reservoir construction,and fracture *** 2019,China Geological Survey(CGS)initiated a demonstration project of HDR exploration and production in the Gonghe Basin,aiming to overcome the setbacks faced by HDR *** the ensuing four years,the Gonghe HDR project achieved the first power generation in 2021,followed by the second power generation test in *** establishing the primary well group in the initial phase,two directional wells and one branch well were *** progress was made in successfully constructing the targeted reservoir,realizing inter-well connectivity,power generation and grid connection,implementing of the real-time micro-seismic monitoring.A closed-loop technical validation of the HDR exploration and production was ***,many technical challenges remain in the process of HDR industrialization,such as reservoir fracture network characterization,efficient drilling and completion,multiple fracturing treatment,continuous injection and production,as well as mitigation of induced seismicity and numerical simulation technology.

关键词： Power generation Hot dry rock(HDR) Enhanced geothermal system(EGS) Genesis mechanism Gonghe Basin Directional drilling Reservoir construction Circulation test Induced seismicity Clean energy exploration engineering

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Analysis and suppression of operational overvoltage and inrush current for high-speed trains by automatic phase-switching technique

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High Voltage 2024年第3期9卷 733-748页

作者： Ye Cao Song Xiao Zhizong Ye Guangning Wu Yujun Guo Guoqiang Gao Chuanming Sun Jiancheng Liu Zheng Chen Hao Hou Zijing wang Puyang Liu Yuhui zhang Jie Yu School of Electrical Engineering Southwest Jiaotong UniversityChengduChina Guangdong Power Grid Limited Liability Company China Southern Power GridGuangzhouChina CRRC Qingdao Sifang Co. Ltd.QingdaoChina State Grid Chongqing Electric Power Company ChongqingChina

When a high-speed train approaches the insulated phase-splitting section embedded between neighbouring power supply arms,the vacuum circuit breaker(VCB)installed on trains must be disconnected to maintain the traction power supply system as a no-load condition for completing phase-switching action,as the train passes through the phase-splitting section depending on its ***,when operating VCBs,the arc is easily triggered between the mobile contacts inside,accompanied by an overvoltage ***,to explore the generating mechanism of inrush current and operational overvoltage,a model describing the‘substation-catenary-train’traction power supply system is launched based on an equivalent circuit modelling *** the analysis of the transient VCB operational process,the phase of catenary voltage is directly related to the characteristics of the VCB switching-on overvoltage,as the traction cur-rent's phase angle is relevant to the amplitude-frequency characteristics of the VCB switching-off *** current as a noteworthy element is related to the traction transformer's *** automatic phase-switching technique is utilised to suppress both operational overvoltage and inrush current,as the optimal combination of VCB switching-on and switching-off phases considering the balance between overvoltage and inrush current is achieved based on the particle swarm algorithm.

关键词： traction phase inrush

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Differential Occupational Health Risks between Methylated PAHs and PAHs:Monitoring 126 PAHs and 6 Oxidative Stress Markers in Paired Serum−Urine Samples

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Environment & Health 2024年第3期2卷 150-160页

作者： Ke-Xin Zhao Chao Jiang Yue Han Wen-Long Li Yu-Wei Li Li-Yan Liu Wan-Li Ma Ying-hua Hu Zi-Feng zhang International Joint Research Center for Persistent Toxic Substances(IJRC-PTS) State Key Laboratory of Urban Water Resource and Environment/School of EnvironmentHarbin Institute of Technology(HIT)Harbin 150090China Wadsworth Center New York State Department of Healthand Department of Environmental Health SciencesSchool of Public HealthState University of New York at AlbanyAlbanyNew York 12237United States

The hazards of polycyclic aromatic hydrocarbons(PAHs)on occupationally exposed population have been widely ***,the occupational exposure risks associated their derivatives,methylated PAHs,remain poorly *** study conducted a screen of 126 PAHs and 6 oxidative stress biomarkers(OSBs)in paired serum−urine samples from 110 petrochemical workers to assess the risk associated with different PAHs *** results showed that the median concentrations of unmetabolized 16 priority PAHs(p-PAHs),16 regular PAHs(R-PAHs),50 methyl-PAHs(Me-PAHs),and 30 nitro-PAHs(N-PAHs)in serum(urine)were 97.98(66.46),11.02(0.00),77.76(31.77),and 1.93(0.10)ng/mL,*** median concentration of metabolized hydroxy PAHs(OH-PAHs)in urine was 12.00 ng/mL(9.49 ng/mg creatinine).OSBs indicate that the hazards of Me-PAHs on exposed populations manifest as protein damage,while the hazards of p-PAHs mainly result in lipid and DNA *** from common diseases and PAH exposure demonstrate a correlation between liver damage and PAH exposure,and Me-PAHs are more difficult to metabolize through urine due to their stronger *** study suggests that traditional health screenings targeting p-PAHs may be insufficient and likely underestimate the exposure risks for occupational populations.

关键词： Petrochemical workers Methyl-PAHs Nitro-PAHs Oxidative stress biomarkers Health risk

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