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Supplementation of Lycium barbarum residue increases the growth rate of Tan sheep by enhancing their feed intake and regulating their rumen microbiome and metabolome

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Journal of Integrative Agriculture 2024年第9期23卷 3129-3144页

作者： Yajun zhang Xiao Chang Bing Wang Dawei Wei Rongzhen Zhong Yansheng Guo Min Du guijie zhang College of Forestry and Prataculture Ningxia UniversityYinchuan 750021China Jilin Provincial Key Laboratory of Grassland Farming Northeast Institute of Geography and AgoecologyChinese Academy of SciencesChangchun 130102China State Key Laboratory of Animal Nutrition/College of Animal Science and Technology China Agricultural UniversityBeijing 100193China College of Animal Science and Technology Ningxia UniversityYinchuan 750021China Nutrigenomics and Growth Biology Laboratory Department of Animal SciencesWashington State UniversityPullmanWA 99164USA

Lycium barbarum residue(LBR),a by-product of *** processing,is packed with bioactive components and can be potentially utilized as a feed additive in animal ***,the fundamental understanding of its effectiveness on livestock animals is still lacking,particularly in *** explore the effects of LBR on the growth performance,rumen fermentation parameters,ruminal microbes and metabolites of Tan sheep,sixteen fattening rams(aged 4 mon)were fed a basal diet(CON,n=8)or a basal diet supplemented with 5%LBR(LBR,n=8).The experiment lasted for 70 d,with 10 d adaptation period and 60 d treatment *** results showed that the LBR enhanced the average daily feed intake,average daily gain(P<0.05),and ruminal total volatile fatty acids(P<0.01)while decreasing ammonia-nitrogen concentration and rumen pH value(P<0.05).Additionally,the LBR improved the relative abundances of Prevotella,Succiniclasticum,Ruminococcus,Coprococcus,Selenomonas,and Butyrivibrio(P<0.05)and reduced the relative abundances of Oscillospira and Succinivibrio(P<0.05).The LBR altered the ruminal metabolome(P<0.01)by increasing the abundances of ruminal metabolites involved in amino acids(e.g.,L-proline,L-phenylalanine,L-lysine,and L-tyrosine),pyrimidine metabolism(e.g.,uridine,uracil,and thymidine),and microbial protein synthesis(e.g.,xanthine and hypoxanthine).In conclusion,LBR had positive effects on the growth rate of Tan sheep as well as on rumen fermentation parameters,rumen microbiome and rumen metabolome.

关键词： Lycium barbarum residue rumen microbiome metabolomics growth performance Tan sheep

The antiviral effect of jiadifenoic acids C against coxsackievirus B3

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Acta Pharmaceutica Sinica B 2014年第4期4卷 277-283页

作者： Miao Ge Huiqiang Wang guijie zhang Shishan Yu Yuhuan Li Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China

Coxsackievirus B type 3(CVB3)is one of the major causative pathogens associated with viral meningitis and myocarditis,which are widespread in the human population and especially prevalent in neonates and *** infections can result in dilated cardiomyopathy(DCM)and other severe clinical *** are no vaccines or drugs approved for the prevention or therapy of CVB3-induced *** screening for anti-CVB3 candidates in our previous studies,we found that jiadifenoic acids C exhibited strong antiviral activities against CVB3 as well as other strains of Coxsackie B viruses(CVBs).The present studies were carried out to evaluate the antiviral activities of jiadifenoic acids *** showed that jiadifenoic acids C could reduce CVB3 RNA and proteins synthesis in a dose-dependent *** acids C also had a similar antiviral effect on the pleconaril-resistant variant of *** further examined the impact of jiadifenoic acids C on the synthesis of viral structural and non-structural proteins,finding that jiadifenoic acids C could reduce VP1 and 3D protein production.A time-course study with Vero cells showed that jiadifenoic acids C displayed significant antiviral activities at 0–6 h after CVB3 inoculation,indicating that jiadifenoic acids C functioned at an early step of CVB3 ***,jiadifenoic acids C had no prophylactic effect against *** together,we show that jiadifenoic acids C exhibit strong antiviral activities against all strains of CVB,including the pleconaril-resistant *** study could provide a significant lead for anti-CVB3 drug development.

关键词： CVB3 Jiadifenoic acids C Antiviral activity

Green tea polyphenols supplementation alters immunometabolism and oxidative stress in dairy cows with hyperketonemia

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Animal Nutrition 2021年第1期7卷 206-215页

作者： Yanfen Ma Ying Feng Liwen Song Muyang Li Hongyu Dai Hua Bao guijie zhang Lei Zhao Chunhua zhang Jing Yi Yusheng Liang School of Agriculture Ningxia UniversityYinchuan 750021China Institute of Animal Nutrition and Feed Inner Mongolia Academy of Agriculture and Animal Husbandry SciencesHohhot 010031China College of Food Engineering&Biotechnology Tianjin University of Science&TechnologyTianjin 300457China College of Animal Science and Veterinary Medicine Heilongjiang Bayi Agricultural UniversityDaqing 163316China College of Veterinary Medicine Nanjing Agricultural UniversityNanjing 210095China Department of Animal Sciences and Division of Nutritional Sciences University of IllinoisUrbana 61801USA

Peripartal cows often experience negative energy balance,and are therefore prone to suffering from metabolic diseases such as hyperketonemia,which causes financial losses in dairy *** study aimed to investigate the effect of green tea polyphenol(GTP)supplementation during the periparturient period on production performance,oxidative stress and immunometabolism in dairy cows with *** hundred Holstein cows were assigned to GTP(0.2 g/kg DM;n=50)or control(without GTP;n=50)group based on body weight,previous milk yield,and parity on d 15 before expected ***,10 cows with hyperketonemia were selected from each group,according to bloodβ-hydroxybutyric acid(BHBA)concentration between 1.2 and 2.9 mmol/L from 2 to 3 d *** cows were fed a close-up diet and a lactation diet with or without GTP supply from 15 d prepartum until 30 d *** and blood samples were obtained from 20 cows selected with hyperketonemia on 10,20,and 30 d *** with control cows,greater milk yield and lower somatic cell count were observed in GTP *** GTP group had lower concentrations of BHBA,free fatty acids,cholesterol,triglyceride,reactive oxygen species,malondialdehyde,and hydrogen peroxide,greater concentrations of glucose,lower activities of aspartate aminotransferase,alanine aminotransferase,and glutamyl transpeptidase,alongside greater ac-tivities of superoxide dismutase,glutathione peroxidase,and total antioxidant ***,GTP supplementation up-regulated concentrations of interleukin-6 and interleukin-10,but down-regulated concentrations of tumor necrosis factor-α,interleukin-1β,interleukin-2,interleukin-8,and interferon-γin *** concentrations of plasma immunoglobulin G were also detected in the GTP ***,the data suggested that GTP supplementation from 15 d prepartum to 30 d postpartum improved the milk yield and health status in cows with hvoerketonemia during early lactation.

关键词： Green tea polyphenol Hyperketonemia Peripartal period Dairy cow

Effect of Process Parameters on Hydroforming of Stainless Steel Tubular Components with Rectangular Section

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Effect of Process Parameters on Hydroforming of Stainless St...

The appropriate standardized ileal digestible tryptophan to lysine ratio improves pig performance and regulates hormones and muscular amino acid transporters in late finishing gilts fed low-protein diets

中国材料大会2012

作者： Yong Xu Shihong zhang Qingxun Zhu Ming Cheng Hongwu Song guijie zhang Institute of Metal Research Chinese Academy of Sciences Metallurgy and Energy College Hebei United University

ISBN: (纸本)9783037856093

The effects of key process parameters including initial lengths of tube blank,forming velocity and loading paths on hydroformability of stainless steel tubular components with rectangular section were systematically *** results showed that sufficient axial feed must be given before the tube contacting the surface of die in order to prevent the excessive thickness thinning,especially for longer tube *** lower loading velocity led to enhanced *** is more important that pulsating loading path observably improved the formability of 304 stainless steel.

关键词： Tube hydroforming Formability Rectangular section Loading paths Stainless steel.

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The appropriate standardized ileal digestible tryptophan to ...

不同蜡样芽孢杆菌对玉米籽粒中黄曲霉毒素B1、玉米赤霉烯酮和呕吐毒素脱毒效果及营养成分评价

第七届中国畜牧科技论坛

作者： Ma wenfeng zhang shihai Zeng xiangfang LIU xutong Xie Chunyuan zhang guijie Qiao shiyan College of Animal Science and Technology Henan University of Science and Technology State Key Laboratory of Animal Nutrition China Agricultural University

This study investigated the effects of various standardized ileal digestible(SID)Trp to Lys ratios on the performance and carcass characteristics of late finishing gilts receiving low CP(9.6%) diets supplemented with crystalline *** gilts(89.1 ± 5.1 kg) were utilized in a dose-response study conducted for 35 *** Trp(0,0.1,0.2,0.4 or0.6 g/kg) was added to a corn-wheat bran basal diet providing SID Trp to Lys ratios of 0.12,0.15,0.18,0.21 or *** diet was fed to 6 pens of pigs with 3 gilts per *** the end of experiment,30 gilts(one pig per pen) were slaughtered to evaluate carcass traits and meat quality(BW = 121 kg).Increasing the SID Trp to Lys ratio increased ADG(linear and quadratic effect,P<0.05) and also improved G:F(linear and quadratic effect,P<0.05).Serum urea nitrogen(SUN) decreased as the SID Trp to Lys ratio increased(linear and quadratic effects,P<0.05).A quadratic effect of L* light and marbling in the Longissimus dorsi was observed as the dietary SID Trp to Lys ratio increased(P<0.05).Increasing the SID Trp to Lys ratio increased the level of serum GH(quadratic effect,P<0.05) and also increased the level of serum insulin-like growth factor(IGF-1)(linear andquadratic effect,P<0.05).Increasing the SID Trp to Lys ratio increased the protein abundance of the muscular amino acid transporter of sodium-coupled neutral amino acid transporter 2(SNAT2) in the Longissimus dorsi muscle(linear and quadratic effect,P<0.05).The optimum SID Trp to Lys ratios to maximize ADG and G:F as well as to minimize SUN levels were 0.16,0.17 and 0.16 using a linear-break point model and 0.20,0.20 and 0.20 using a quadratic *** may regulate performance by altering the levels of muscular amino acid SNAT2 transporters through the up-regulation of hormones such as GH and IGF-1.

关键词： amino acid transporters gilts hormones low-protein performance tryptophan

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饲料工业 2022年第23期43卷 38-43页

作者：石楠张桂杰宁夏大学农学院宁夏银川750021

试验旨在评估可有效降低玉米籽粒中黄曲霉毒素B1(Aflatoxin B1,AFB1)、玉米赤霉烯酮(Zearalenone,ZEN)和呕吐毒素(Deoxynivalenol,DON)含量的优良菌株。试验以霉菌毒素超标的玉米籽粒作为脱毒材料,设置4个处理组:CK(对照组);TD-1蜡样芽... 详细信息

试验旨在评估可有效降低玉米籽粒中黄曲霉毒素B1(Aflatoxin B1,AFB1)、玉米赤霉烯酮(Zearalenone,ZEN)和呕吐毒素(Deoxynivalenol,DON)含量的优良菌株。试验以霉菌毒素超标的玉米籽粒作为脱毒材料,设置4个处理组:CK(对照组);TD-1蜡样芽孢杆菌处理组(1%、5%、10%);TD-2蜡样芽孢杆菌处理组(1%、5%、10%);TD-3蜡样芽孢杆菌处理组(1%、5%、10%)。在玉米籽粒发酵后48 h进行取样,测定AFB1、DON和ZEN含量及营养成分。结果表明:TD-1蜡样芽孢杆菌对AFB1、DON和ZEN脱毒效果最优。添加5%的TD-1蜡样芽孢杆菌对玉米籽粒中AFB1和DON脱毒率分别为67.59%和73.03%;添加10%的TD-1蜡样芽孢杆菌菌液发酵48 h后,对ZEN脱毒率可达65.71%;添加蜡样芽孢杆菌菌液会降低玉米籽粒可溶性碳水化合物(Water soluble carbohydrate,WSC)含量,对其他营养成分无显著影响(P>0.05)。

关键词：蜡样芽孢杆菌玉米籽粒黄曲霉毒素B1 玉米赤霉烯酮呕吐毒素

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米糠和乳酸菌制剂对柠条锦鸡儿青贮发酵品质及微生物多样性的影响

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动物营养学报 2022年第3期34卷 1800-1808页

作者：张欢朱鸿福闫艳红张桂杰宁夏大学动物科学系银川750021 宁夏草牧业工程技术研究中心银川750021 四川农业大学动物科技学院成都611130

本试验旨在研究米糠和乳酸菌制剂对柠条锦鸡儿青贮发酵品质及微生物多样性的影响,以期为改善柠条锦鸡儿青贮发酵品质和解决干旱地区饲草料短缺问题提供科学依据。试验设4个处理,分别为无添加(对照组)、添加5%米糠(RB组)、添加5%米糠+10;... 详细信息

本试验旨在研究米糠和乳酸菌制剂对柠条锦鸡儿青贮发酵品质及微生物多样性的影响,以期为改善柠条锦鸡儿青贮发酵品质和解决干旱地区饲草料短缺问题提供科学依据。试验设4个处理,分别为无添加(对照组)、添加5%米糠(RB组)、添加5%米糠+10;CFU/g商业植物乳杆菌(RB+LP组)和添加5%米糠+10;CFU/g植物乳杆菌LP694(RB+LP694组)。发酵60 d后,测定柠条锦鸡儿青贮的营养成分、发酵品质及微生物多样性。结果表明:1)与对照组相比,RB、RB+LP和RB+LP694组青贮饲料中性洗涤纤维和酸性洗涤纤维含量均显著降低(P<0.05),RB和RB+LP694组青贮饲料中pH显著降低(P<0.05),RB+LP694组青贮饲料中氨态氮/总氮(NH;-N/TN)显著降低(P<0.05),RB、RB+LP和RB+LP694组青贮饲料中乳酸含量及乳酸/乙酸均显著提高(P<0.05)。2)RB+LP组Shannon指数和Chao1指数显著高于对照、RB和RB+LP694组(P<0.05)。3)各组青贮饲料中优势菌门均为厚壁菌门(Firmicutes),其次为变形菌门(Proteobacteria);优势菌属均为乳杆菌属(Lactobacillus)。综上所述,本试验中,添加5%米糠+10;CFU/g植物乳杆菌LP694改善柠条锦鸡儿青贮发酵品质和微生物组成的效果最好。

关键词：柠条锦鸡儿米糠乳杆菌微生物多样性

不同添加量枸杞副产物对柠条锦鸡儿发酵特性及微生物多样性的影响

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动物营养学报 2021年第9期33卷 5152-5161页

作者：牟怡晓张欢马聪慧张桂杰宁夏大学农学院动物科学系银川750021 宁夏草牧业工程技术研究中心银川750021

本试验旨在探究不同添加量枸杞副产物对柠条锦鸡儿发酵特性及微生物多样性的影响。按单因素完全随机设计分为4个组,每组6个重复。各组柠条锦鸡儿青贮中枸杞副产物添加量分别为0(对照组)、3%(L3组)、6%(L6组)和9%(L9组)。青贮60 d后,测... 详细信息

本试验旨在探究不同添加量枸杞副产物对柠条锦鸡儿发酵特性及微生物多样性的影响。按单因素完全随机设计分为4个组,每组6个重复。各组柠条锦鸡儿青贮中枸杞副产物添加量分别为0(对照组)、3%(L3组)、6%(L6组)和9%(L9组)。青贮60 d后,测定柠条锦鸡儿青贮的营养成分、发酵品质、有氧稳定性和微生物多样性。结果表明:1)L6组和L9组多糖含量显著高于对照组(P<0.05),中性洗涤纤维(NDF)含量显著低于对照组(P<0.05);L9组酸性洗涤纤维(ADF)含量显著低于其他各组(P<0.05),粗蛋白质、粗脂肪含量及相对饲喂价值(RFV)显著高于其他各组(P<0.05)。2)L9组pH显著低于对照组(P<0.05),L6组和L9组氨态氮/总氮显著低于对照组(P<0.05),L9组乳酸含量显著高于其他各组(P<0.05),L6组和L9组乙酸和丙酸含量显著低于对照组(P<0.05)。3)L6组和L9组有氧稳定性显著高于对照组(P<0.05)。4)L3组、L6组和L9组Chao指数和ACE指数均显著高于对照组(P<0.05),L3组和L9组Shannon指数显著高于对照组(P<0.05),L3组和L9组Simpson指数显著低于对照组(P<0.05)。5)各组乳杆菌属(Lactobacillus)相对丰度最高,片球菌属(Pediococcus)和肠球菌属(Enterococcus)相对丰度次之。与对照组相比,L3组、L6组和L9组的微生物多样性均有所增加。综上所述,枸杞副产物可改善柠条锦鸡儿青贮的营养价值和发酵品质,改变微生物群落组成。本试验条件下,枸杞副产物添加量为9%时青贮效果最好。

关键词：柠条锦鸡儿枸杞副产物青贮营养成分发酵品质微生物

不可消化中性洗涤纤维在反刍动物生产中的应用及研究进展

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动物营养学报 2020年第11期32卷 5069-5074页

作者：牟怡晓林语梵张桂杰宁夏大学动物科学系银川750021

饲草中性洗涤纤维(NDF)能够为反刍动物提供能量,适当的NDF含量有利于提高动物生产性能。根据NDF可消化程度将其分为潜在可消化中性洗涤纤维(pdNDF)和不可消化中性洗涤纤维(iNDF)。iNDF可用于估测反刍动物采食量及能量摄入,还可以作为预... 详细信息

饲草中性洗涤纤维(NDF)能够为反刍动物提供能量,适当的NDF含量有利于提高动物生产性能。根据NDF可消化程度将其分为潜在可消化中性洗涤纤维(pdNDF)和不可消化中性洗涤纤维(iNDF)。iNDF可用于估测反刍动物采食量及能量摄入,还可以作为预测有机物消化率的重要指标。本文总结了iNDF定义、测定方法及其对反刍动物生产调控的作用,并对近红外光谱法预测饲草中iNDF含量进行了展望,以期为iNDF在反刍动物生产中的应用提供借鉴和参考。

关键词：不可消化中性洗涤纤维中性洗涤纤维消化率反刍动物近红外光谱法