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miR-125b-5p and miR-99a-5p downregulate human γδ T-cell activation and cytotoxicity

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Cellular & Molecular Immunology 2019年第2期16卷 112-125页

作者： Yuli Zhu siya zhang Zinan Li Huaishan Wang Zhen Li Yu Hu Hui Chen Xuan zhang Lianxian Cui Jianmin zhang Wei He Department of Immunology Research Center on Pediatric Development and DiseasesInstitute of Basic Medical SciencesChinese Academy of Medical Sciences and School of Basic MedicinePeking Union Medical CollegeState Key Laboratory of Medical Molecular BiologyBeijing 100005China Institute of blood transfusion Qingdao Blood CenterQingdao 266071China Department of Rheumatology&Clinical Immunology Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China

As an important component of innate immunity,human circulatingγδT cells function in rapid responses to infections and ***(miRNAs)play a critical regulatory role in multiple biological processes and ***,how the functions of circulating humanγδT cells are regulated by miRNAs merits *** this study,we profiled the miRNA expression patterns in human peripheralγδT cells from 21 healthy donors and identified 14 miRNAs that were differentially expressed between peripheralαβT cells andγδT *** the 14 identified genes,7 miRNAs were downregulated,including miR-150-5p,miR-450a-5p,miR-193b-3p,miR-365a-3p,miR-31-5p,miR-125b-5p and miR-99a-5p,whereas the other 7 miRNAs were upregulated,including miR-34a-5p,miR-16-5p,miR-15b-5p,miR-24-3p,miR-22-3p,miR-22-5p and miR-9-5p,inγδT cells compared withαβT *** subsequent functional studies,we found that both miR-125b-5p and miR-99a-5p downregulatedγδT cell activation and cytotoxicity to tumor *** of miR-125b-5p or miR-99a-5p inγδT cells inhibitedγδT cell activation and promotedγδT cell ***,miR-125b-5p knockdown facilitated the cytotoxicity ofγδT cells toward tumor cells in vitro by increasing degranulation and secretion of IFN-γand TNF-α.Our findings improve the understanding of the regulatory functions of miRNAs inγδT cell activation and cytotoxicity,which has implications for interventional approaches toγδT cell-mediated cancer therapy.

关键词： γδT cells miR-125b-5p miR-99a-5p activation cytotoxicity

Lymphatic endothelial cells regulate B-cell homing to lymph nodes via a NIK-dependent mechanism

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Cellular & Molecular Immunology 2019年第2期16卷 165-177页

作者： Jie Yang siya zhang Lingyun zhang Xiaoping Xie Hui Wang Zuliang Jie Meidi Gu Jin-Young Yang Xuhong Cheng Shao-Cong Sun Department of Immunology The University of Texas MD Anderson Cancer CenterHouston TX 77030USA Department of Immunology Institute of Basic Medical SciencesChinese Academy of Medical Sciences and School of Basic MedicinePeking Union Medical CollegeState Key Laboratory of Medical Molecular BiologyBeijing 100005China The University of Texas Graduate School of Biomedical Sciences HoustonTX 77030USA

B cells home to the lymph nodes(LNs)via high endothelial venules(HEVs)under the guidance of chemokines,particularly ***,as CXCL13 is not directly made in HEVs,the molecular mechanism mediating B-cell homing to LNs has remained *** show here that nuclear factor(NF)-κB-inducing kinase(NIK),a kinase mediating activation of the noncanonical NF-κB pathway,functions in lymphatic endothelial cells(LECs)to regulate B-cell homing to ***-conditional deletion of NIK in mice did not affect the integrity or global function of lymphatic vessels but caused a severe reduction in the frequency of B cells in *** LEC-specific NIK deficiency did not affect the survival of B cells or the frequency of B cells in the spleen.B-cell adoptive transfer studies revealed that the LEC-specific NIK deletion impairs the ability of LNs to recruit B *** further show that NIK mediates expression of the chemokines CXCL13 and CCL19 in *** CCL19 is also expressed in blood endothelial cells(BECs),CXCL13 is not produced in *** results suggest that NIK regulates naive B-cell homing to LNs via mediating production of the B-cell homing chemokine CXCL13 in LECs.

关键词： B-cell homing lymphatic vessel NIK noncanonical NF-κB

Computational Insights into the Mechanism and Regulation of Membrane Domain Registration/Anti-registration

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Computational Insights into the Mechanism and Regulation of ...

High throughput sequencing uncovers a specific pattern of γδ T cells in patients with SAPHO syndrome

2019中国化学会第十五届全国计算（机）化学学术会议

作者：林绪波 siya zhang 北京航空航天大学 State Key Laboratory of Medical Molecular Biology School of Basic Medicine Peking Union Medical College and Institute of Basic Medical Sciences Chinese Academy of Medical Sciences

Due to differential interactions between different lipids and proteins, plasma membrane can segregate into various dynamical nanoscale domains. Lipid rafts, nanoscale ordered functional membrane domains play key roles in many biological processes. Many efforts have been made to understand the inner-leaflet and inter-leaflet dynamics of lipid rafts and their effects on membrane proteins. The mechanism for the inner-leaflet dynamics is relatively clear. However, the comprehensive understanding of the mechanism for the inter-leaflet dynamics（membrane domain registration/anti-registration） is still lacking. Here, we will show our recent progresses on identifying key factors responsible for the inter-leaflet coupling, which determines the membrane domain registration/anti-registration process. Then, the possible biological effects of membrane domain anti-registration on the intrinsic membrane potential and the function of ion channels will be discussed. Finally, the regulation of the membrane domain registration/anti-registration via nanoparticles will be proposed. We believe these results from our molecular dynamics simulations will provide useful insights for the inter-leaflet dynamics of lipid rafts.

关键词： Molecular Dynamics Simulation Membrane Domain Dynamics Nanoparticles

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High throughput sequencing uncovers a specific pattern of γ...

Study on the role of TRIM69 in lymphocytes development and macrophage function

第十届全国免疫学学术大会

作者： siya zhang Hui Chen Wei He Department of Immunology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences and School of Basic Medicine Peking Union Medical College State Key Laboratory of Medical Molecular Biology

Objective:The etiology of SAPHO(acronym for synovitis,acne,pustulosis,hyperostosis and osteitis)syndrome may involves infection and immune disorder.γδT cells play important roles in innate immunity as the first-line of defense against infectious ***,they’re the main source of *** this study,we analyzed phenotype ofγδT cells and performed high throughput sequencing of CDR3(complementary determining region 3)ofγδTCR in Chinese patients with SAPHO ***:42 patients with SAPHO syndrome and 16 healthy controls were involved in this *** accordance with marks of VAS(Visual Analogue Scale)pain and BASDAI(Bath Ankylosing Spondylitis Activity Index),SAPHO patients were divided into active group(n=18)and stable group(n=24).Informed consent was obtained from all *** means of flow cytometry,the proportions of total and different subsets ofγδT cells in PBMCs(peripheral blood mononuclear cells)of different groups of SAPHO syndrome and healthy controls were ***,12age and gender matched RNA samples which equally came from three groups’PBMCs were selected to proceeding high throughput sequencing of CDR3 ofγδ***:In patients with SAPHO syndrome,VAS scores and BASDAI marks were positively correlated with ESR(Erythrocyte Sedimentation Rate)(PVAS﹤0.0001,r=0.6943;PBASDAI=0.001,r=0.4885)and hs CRP(hypersensitivity C Reactive Protein)(PVAS﹤0.0001,r=0.6825;PBASDAI=0.001,r=0.4906).No difference with total and subsets ofγδT cells(Vδ1γδT cells,Vδ2γδT cells,CD27+γδT cells and CD27-γδT cells)among three ***,the balance between Vδ1γδT cells and Vδ2γδT cells was disrupted in active group comparing with stable group of SAPHO syndrome(P=0.0082).Conclusions:The data provide a role ofγδT cells in the pathogenesis of SAPHO syndrome.

关键词： SAPHO syndrome γδ T cells High throughput sequencing

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Study on the role of TRIM69 in lymphocytes development and m...

跨语言标注情境中的学习行为——基于推荐标签的实验分析

第九届全国免疫学学术大会

作者： Ji Xu He Kangxia zhang siya zhang Jianmin He Wei Department of Immunology State Key Laboratory of Medical Molecular BiologySchool of Basic MedicinePeking Union Medical College Institute of Basic Medical Sciences

Background:TRIM69,an E3 ubiquitin ligase which contains classical RBCC domians,is a canonical tripartite motif containing protein and could induce apoptosis in cells when *** TRIM69 has a role in lymphocytes development and macrophage function remains ***:Examine the role of TRIM69 in lymphocytes development and macrophage function in ***:Single cell suspension was isolated from lymphoid nodes(LNs),spleens and thymuses from TRIM69 knocked out(KO)as well as wild type(WT)mice by passing through 40μm cell *** cytometry was employed to test the subgroup *** macrophage was isolated from WT and KO mice and was seeded in 24 well plates(3×10/well)and 6 well plates(1×10/well)and stimulated with 100ng/ml LPS,and the cell lysate and culture supernatant were collected and tested by WB and ***:(1)We found the TRIM69mice has the normal breed capacity,and the sizes and proportion of B cells,T cells,CD4,CD8T cells in spleens,thymuses and LNs were normal compared with the WT mice;(2)the proportion of CD25CD44T cells in the thymus of TRIM69mice were much higher than WT mice;(3)the phospho-NF-KB in macrophage stimulated with LPS were much more in TRIM69mice than that in WT ***:TRIM69mice displayed normal proportions of T cells,B cells,αβT cells andγδT cells in spleens,LNs and IELs,but had much more CD25CD44T cells in thymuses,suggesting a regulatory role in T cell development As many other Tripartite motif containing proteins,TRIM69 may have a negative role in innate immunity.

关键词： TRIM69 T cell development Intestinal intraepithelial lymphocytes

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图书情报知识 2020年第4期37卷 76-86页

作者：张思雅马晓悦赵捧未西安电子科技大学西安710071 西安交通大学西安710049

[目的/意义]厘清跨语言环境中推荐标签及其表征语言对用户标注学习行为的影响,是优化和提高推荐标签质量、改善用户跨语言标注体验的重要基础。[研究设计/方法]通过受控实验的方法对比用户在外文推荐标签、中文推荐标签以及无推荐标签... 详细信息

[目的/意义]厘清跨语言环境中推荐标签及其表征语言对用户标注学习行为的影响,是优化和提高推荐标签质量、改善用户跨语言标注体验的重要基础。[研究设计/方法]通过受控实验的方法对比用户在外文推荐标签、中文推荐标签以及无推荐标签的情况下的跨语言标注结果和学习行为。[结论/发现]跨语言标注环境下存在三种基于推荐标签的学习模式:基于标签形式的学习、基于标签语义的长语义映射学习和基于标签语义的短语义映射学习;推荐标签在为用户的标注学习提供指导的同时,也带来一定的代价,这符合"锚定效应"的观点。[创新/价值]研究结果将有助于指导跨语言标注系统的设计与实现,提高信息共享效率。

关键词：跨语言推荐标签学习行为信息标注

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数字时代金融数据风险及治理研究

市场周刊 2023年第10期36卷 137-140,144页

作者：张斯雅湖南工业大学经济与贸易学院湖南株洲412000

数据是数字经济中的关键生产要素,金融市场作为数据密集型行业拥有大量的数据,这些数据在带来极大价值的同时也容易遭到网络攻击。因此,提升金融市场中的数据治理水平,对防范金融市场中的系统性风险和金融业的良性发展具有重要意义。文... 详细信息

数据是数字经济中的关键生产要素,金融市场作为数据密集型行业拥有大量的数据,这些数据在带来极大价值的同时也容易遭到网络攻击。因此,提升金融市场中的数据治理水平,对防范金融市场中的系统性风险和金融业的良性发展具有重要意义。文章在对金融数据风险及其成因进行分析的基础上,对我国在金融数据治理方面面临的挑战进行了研究,并以我国金融市场发展实际为依据,提出了在数字时代加速金融数据风险防控与治理的相关建议。

关键词：数字时代金融数据金融安全

内外侧分区设计在小阴唇及阴蒂包皮复合增生治疗中的应用效果

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中华整形外科杂志 2022年第8期38卷 881-886页

作者：张思娅陈凤超李峰永首都医科大学附属北京友谊医院医学美容中心北京100050 中国医学科学院北京协和医学院整形外科医院整形十科北京100144

目的探讨内外侧分区设计在小阴唇及阴蒂包皮复合增生治疗中的应用效果。方法回顾性分析2020年1月至2022年2月首都医科大学附属北京友谊医院医学美容中心收治的小阴唇及阴蒂包皮复合增生患者的临床资料。患者小阴唇内侧面垂直方向上无松... 详细信息

目的探讨内外侧分区设计在小阴唇及阴蒂包皮复合增生治疗中的应用效果。方法回顾性分析2020年1月至2022年2月首都医科大学附属北京友谊医院医学美容中心收治的小阴唇及阴蒂包皮复合增生患者的临床资料。患者小阴唇内侧面垂直方向上无松垂,外侧面表现出松垂、褶皱,同时合并阴蒂包皮增生。采用内外侧分区设计,将小阴唇外侧面及阴蒂包皮视为整体,设计楔形切口线,拟将其垂直方向上多余组织去除,在此基础上,设计外侧面弧形切口线,再将其投影至小阴唇内侧面,形成小阴唇内侧面弧形切口线,结合楔形切除法和边缘切除法进行小阴唇及阴蒂包皮复合增生的整形手术。随访观察患者术后并发症、小阴唇及阴蒂包皮形态对称情况、摩擦不适感改善情况、小阴唇及阴蒂包皮较术前清洁情况、患者对小阴唇及阴蒂包皮形态的满意度、术后性满足感有无变化。结果共纳入17例女性患者,年龄20~35岁,平均27岁。术后随访2个月,切口均一期愈合,切缘无锯齿样畸形,阴蒂包皮及小阴唇外形自然,患者自觉双侧小阴唇及阴蒂包皮完全对称3例、基本对称12例、部分不对称2例,2例部分不对称患者不要求进一步手术修复;17例患者摩擦不适感均完全改善,小阴唇及阴蒂包皮均较术前更易清洁。患者对外形非常满意5例、满意10例、不满意2例。有性生活经历者15例,10例术后性满足感提升,5例无变化。结论通过内外侧分区设计,采用楔形节除法和边缘切除法治疗小阴唇及阴蒂包皮复合增生,可在单次手术中解决小阴唇及阴蒂包皮增生所导致的摩擦不适、不易清洁的困扰,同时取得较好的美学效果。

关键词：外科,整形小阴唇阴蒂包皮小阴唇缩小整形术

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基于Fu-Liou辐射传输模式和卫星观测资料的气溶胶对全球地表太阳辐射影响的研究

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资源科学 2017年第3期39卷 513-521页

作者：邵思雅张晶周丽花薛文晧北京师范大学全球变化与地球系统科学研究院北京100875 全球变化研究协同创新中心北京100875

气溶胶可以吸收和散射太阳辐射,影响地表辐射收支平衡,使到达地表的太阳总辐射减少,同时散射辐射在总辐射中所占的比例增加。本文基于MODIS气溶胶产品和CERES云产品等大气遥感资料,利用Fu-Liou辐射传输模式对全球地表辐射进行了有无气... 详细信息

气溶胶可以吸收和散射太阳辐射,影响地表辐射收支平衡,使到达地表的太阳总辐射减少,同时散射辐射在总辐射中所占的比例增加。本文基于MODIS气溶胶产品和CERES云产品等大气遥感资料,利用Fu-Liou辐射传输模式对全球地表辐射进行了有无气溶胶的两组模拟试验。将考虑气溶胶的试验模拟值与BSRN站点观测值进行对比,结果显示地表总辐射模拟的R值达到0.92,地表散射辐射模拟的R值达到0.84,模拟值与观测值有着比较好的一致性。比较两组模拟结果显示,2007年气溶胶导致全球地表总辐射平均减少了9.16W/m2,降低幅度较大的地区往往气溶胶光学厚度(AOD)也较高。对于散射辐射来说,气溶胶在全球不同地区却有着不同的正负效应:地表反照率高的地区,气溶胶往往使得散射辐射大幅增加;而云分数较高的地区,气溶胶往往导致散射辐射大幅度下降。2007年气溶胶导致全球地表散射辐射平均增加了8.17W/m2。

关键词：气溶胶气溶胶光学厚度大气辐射传输直接辐射效应散射