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Penumbra-targeted CircOGDH siRNA-loaded nanoparticles alleviate neuronal apoptosis in focal brain ischaemia

Stroke & Vascular Neurology 2024年第2期9卷 134-144,I0396-I0413页

作者： Yanfang Liu Tianyuan zhang Xing Zou Zhongwen Yuan Yufeng Li Jiankun Zang Niu He Lizhen He Anding Xu Dan Lu department of Neurology and Stroke Center Jinan University First Affiliated HospitalGuangzhouGuangdongChina department of Clinical Neuroscience Institute The First Affiliated Hospital of Jinan UniversityGuangzhouGuangdongChina Key Lab of Guangzhou Basic and Translational Research of Pan-vascular Diseases The First Affiliated Hospital of Jinan UniversityGuangzhouChina

Background Nanoparticles(NPs)are a class of substances that can be loaded with therapeutic agents delivered to specific *** our earlier research,we identified a neuron-derived circular RNA(circRNA),circular oxoglutarate dehydrogenase(CircOGDH),as a promising therapeutic target for acute ischaemic *** study dedicated to explore a prospective preliminary strategy of CircOGDH-based NP delivered to the ischaemic penumbra region in middle cerebral artery occlusion/reperfusion(MCAO/R)*** Immunofluorescence in primary cortex neurons and in vivo fluorescence imaging revealed endocytosis of Poly(lactide-co-glycolide)(PLGA)poly amidoamine(PAMAM)@CircOGDH small interfering RNA(siRNA)*** blotting analysis and CCK8 assay were performed to evaluate the apoptotic level in ischaemic neurons treated with PLGA–PAMAM@CircOGDH siRNA *** reverse transcription PCR experiments,mice behaviour test,T2.MRI analysis,Nissl and TdT-mediated dUTP nick end labeling(TUNEL)co-staining were performed to evaluate the apoptosis level of ischaemic penumbra neurons in MCAO/R *** evaluation of NPs in MCAO/R mice was detected by blood routine examination,liver and kidney function examination and HE *** PLGA–PAMAM@CircOGDH siRNA NPs were successfully *** of PLGA–PAMAM@CircOGDH siRNA NPs in ischaemic neurons alleviated neuronal apoptotic level in vitro and in ***,mice behaviour test showed that the neurological defects of MCAO/R mice were significantly alleviated after the tail injection of PLGA–PAMAM@CircOGDH siRNA NPs,and no toxic effects were *** In conclusion,our results suggest that PLGA–PAMAM@CircOGDH siRNA NPs can be delivered to the ischaemic penumbra region and alleviate neuron apoptosis in MCAO/R mice and in ischaemic neurons;therefore,our study provides a desirable approach for using circRNA-based NPs for the treatment of ischaemic stroke.

关键词： OGD routine loaded

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能谱纯化Sn 150 kV结合ADMIRE在腰椎CT检查中的价值

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CT理论与应用研究（中英文） 2024年第1期33卷 49-55页

作者：蒋东秦立新武汉市肺科医院放射科武汉430033

目的:研究能谱纯化(SPS)Sn 150 kV结合高级模拟迭代重建(ADMIRE)在腰椎CT检查中的可行性。方法:行腰椎CT检查的患者88例,年龄在2.~65岁,BMI在18.5~2. kg/m^(2.的患者,分成对照组(A组)和试验组(B组),每组44例;A组管电压12. kV,B组管电压S... 详细信息

目的:研究能谱纯化(SPS)Sn 150 kV结合高级模拟迭代重建(ADMIRE)在腰椎CT检查中的可行性。方法:行腰椎CT检查的患者88例,年龄在2.~65岁,BMI在18.5~2. kg/m^(2.的患者,分成对照组(A组)和试验组(B组),每组44例;A组管电压12. kV,B组管电压Sn 150 kV,其他扫描参数均一致,完成检查并比较两组的图像质量和辐射剂量。结果:A组和B组图像客观评价噪声存在统计学差异,A组图像噪声高于B组;主观评价3位诊断者的ICC为0.769,表示评价一致性较好;B组的辐射剂量低于A组30.31%。结论:采用能谱纯化Sn 150 kV结合ADMIRE,不但能有效减低辐射剂量,还可保证优质的图像质量,值得在成人腰椎CT中推广使用。

关键词：高级模拟迭代重建能谱纯化腰椎

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Primary coexisting adenocarcinoma of the colon and neuroendocrine tumor of the duodenum: A case report and review of the literature

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World Journal of Gastrointestinal Surgery 2024年第8期16卷 2724-2734页

作者： Song Fei Wei-Dong Wu Han-Shuo zhang Shao-Jie Liu Dan Li Bo Jin department of Thoracic and Cardiovascular Surgery Guangzhou Red Cross Hospital of Jinan UniversityGuangzhou 510000Guangdong ProvinceChina department of Gastrointestinal Surgery Guangzhou Red Cross Hospital of Jinan UniversityGuangzhou 510000Guangdong ProvinceChina

BACKGROUND Neuroendocrine tumors(NETs)arise from the body’s diffuse endocrine *** primary adenocarcinoma of the colon and NETs of the duodenum(D-NETs)is a rare occurrence in clinical *** classification and treatment criteria for D-NETs combined with a second primary cancer have not yet been *** SUMMARY We report the details of a case involving female patient with coexisting primary adenocarcinoma of the colon and a D-NET diagnosed by imaging and surgical *** tumors were treated by surgery and four courses of *** patient achieved a favorable clinical *** Coexisting primary adenocarcinoma of the colon and D-NET were diagnosed by imaging,laboratory indicators,and surgical *** resection com-bined with chemotherapy was a safe,clinically effective,and cost-effective treat-ment.

关键词： Neuroendocrine tumor of the duodenum Multiple primary tumors Colorectal cancer Endoscopic resection Case report

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Biochar regulates biogeochemical cycling of iron and chromium in a soil-rice system by stimulating Geobacter and Clostridium

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Pedosphere 2024年第5期34卷 929-940页

作者： Min XU Yang LIN Jing MA Lulu LONG Chao CHEN Gang YANG Chun SONG Jun WU Xiaohong zhang Peng GAO College of Environmental Science Sichuan Agricultural University Soil Water and Ecosystem Sciences Department University of Florida College of Water Conservancy and Hydropower Engineering Sichuan Agricultural University department of Environmental and Occupational Health University of Pittsburgh School of Public Health

In soil-rice systems,microbial reduction of iron （Fe） has been recognized as a crucial biogeochemical process that regulates Fe and chromium （Cr）translocation;however,the underlying processes are *** investigate the impacts of biochar on the biochemical cycling of Fe and Cr and their toxicity to rice,maize straw biochar was applied at 1%（weight/weight） to a paddy soil spiked with 300 mg kg-1Cr under two phosphorus （P） levels (0 or 90 mg kg-1) in a pot *** key microbial groups affecting Fe dissimilatory reduction and their environmental drivers were *** inhibited root Cr uptake by 36%,owing to the promoted iron plaque （IP） formation on the rice root *** analysis showed that Fe concentration in pore water was strongly linked to the abundances of Geobacter （r=0.81–0.94,P2.and 32.of the variation in community composition,***,biochar increased Fe and P concentrations in root cell walls,which retained more ***,Cr stress in rice under biochar treatment was relieved through increasing IP formation and altering subcellular *** mechanistic insights had important implications for reducing Cr uptake by rice.

关键词： chromium translocation iron plaque iron reduction maize straw biochar microbial reduction paddy soil phosphorus fertilization subcellular distribution

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Strong additive effect of calcitriol and cyclosporine A on lymphocyte proliferation in vitro and rat liver allotransplantations in vivo

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Chinese Medical Journal 2006年第24期119卷 2090-2095页

作者： zhang Ai-bin and ZHENG Shu-sen department of Hepatobiliary Surgery,First Affiliated Hospital of Medical College,Zhejiang University Key Lab of Combined Multi-Organ Transplantation,Ministry of Health,{2. 310003,china department of Hepatobiliary Surgery First Affiliated Hospital of Medical College Zhejiang University

Background Vitamin D3 and its metabolites have been found to exert immunosuppressive effects both in vivo and in vitro. We investigated the synergistic effect of calcitriol and cyclosporine A （CsA） on lymphocyte proliferation in vitro and graft rejection following rat liver allotransplantations in vivo. Methods Alloantigen driven, human peripheral mononuclear cells＇ proliferation and cytokine production capacity were tested in the presence or absence of various concentrations of calcitriol or CsA. In vivo, liver allografts were transplanted in a high responder strain combination （SD to Wistar） rats and combination of subtherapeutical dose of CsA and calcitriol was administered in recipients, whereas the control recipients received single or no immunosuppressant. Proliferation of splenocyte from recipient was tested with mixed lymphocyte reaction. Serum intedeukin-2.（IL-2. and interferon gamma （IFN-γ） concentrations were measured with enzyme linked immunosorbent assay. Results Combined medication of 10^-9 mol/L calcitriol and 100 ng/ml CsA inhibited human peripheral mononuclear cells＇ proliferation to alloantigen and the production of IL-2.and IFN-γ but promoted that of IL-4 and IL-10. Similarly, combination of 2.0 ng · kg^-1·-d^-1 calcitriol and 1.0 mg · kg^-1·-d^-1 CsA showed an additive effect in liver transplant model. It restrained splenocyte proliferation to alloantigen from donor and significantly reduced serum concentration of IL-2.and IFN-γ in recipients. Consequently, aUograft rejection in combined medication group was minor （median William＇s grade was 1.0 vs 3.0 in combined medication group and in the control group, P〈0.05） and the recipients＇ survival was evidently prolonged [（93.7±5.8） days vs （12.6±1.4） days in combined medication group and in the control group, P〈0.01 ]. Conclusion A combination of calcitriol and CsA has an additive effect on limiting lymphocyte proliferation and prolonging liver graft survival. With its additional immunomodulating pro

关键词： liver transplantation vitamin D cyclosporine cytokine lymphocyte graft rejection

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Role of adhesion molecules in cancer and targeted therapy

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Science china(Life Sciences) 2024年第5期67卷 940-957页

作者： Chunmei Fan Fang Xiong Shanshan zhang Zhaojian Gong Qianjin Liao Guiyuan Li Can Guo Wei Xiong He Huang Zhaoyang Zeng NHC Key Laboratory of Carcinogenesis and Hunan Key Laboratory of Cancer Metabolism Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of MedicineCentral South UniversityChangsha 410000China department of Histology and Embryology Xiangya School of MedicineCentral South UniversityChangsha410013China department of Stomatology Xiangya HospitalCentral South UniversityChangsha 410078China department of Oral and Maxillofacial Surgery The Second Xiangya HospitalCentral South UniversityChangsha 410011China Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education Cancer Research InstituteCentral South UniversityChangsha 410078China

Adhesion molecules mediate cell-to-cell and cell-to-extracellular matrix interactions and transmit mechanical and chemical signals among *** mechanisms deregulate adhesion molecules in cancer,enabling tumor cells to proliferate without restraint,invade through tissue boundaries,escape from immune surveillance,and survive in the tumor *** studies have revealed that adhesion molecules also drive angiogenesis,reshape metabolism,and are involved in stem cell *** this review,we summarize the functions and mechanisms of adhesion molecules in cancer and the tumor microenvironment,as well as the therapeutic strategies targeting adhesion *** studies have implications for furthering our understanding of adhesion molecules in cancer and providing a paradigm for exploring novel therapeutic approaches.

关键词： adhesion molecules tumor microenvironment invasion and metastasis reshaping metabolism angiogenesis immune evasion tumor stemness therapeutic strategy

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白细胞介素-7增强脓毒性心肌病患者辅助性T细胞9活性

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中华急诊医学杂志 2024年第1期33卷 70-77页

作者：张宇厉菁张华张传西郑州市第七人民医院心血管内科郑州450000

目的观察白细胞介素-7(interleukin-7,IL-7)、辅助性T细胞9(T helper cell 9,Th9)、杀伤性T细胞9(cytotoxic T cell 9,Tc9)在脓毒性心肌病患者中的水平,评估外源性IL-7对脓毒性心肌病患者Th9细胞的调控。方法采用横断面研究,纳入2.18年... 详细信息

目的观察白细胞介素-7(interleukin-7,IL-7)、辅助性T细胞9(T helper cell 9,Th9)、杀伤性T细胞9(cytotoxic T cell 9,Tc9)在脓毒性心肌病患者中的水平,评估外源性IL-7对脓毒性心肌病患者Th9细胞的调控。方法采用横断面研究,纳入2.18年6月至2.2.年1月在郑州市第七人民医院就诊的脓毒性心肌病患者、脓毒症患者和对照者,分离血浆和外周血单个核细胞(peripheral blood mononuclear cells,PBMCs),重组IL-7刺激PBMCs,酶联免疫吸附试验检测IL-7、IL-9、可溶型CD12.水平,流式细胞术检测T细胞中CD12.表达和Th9、Tc9细胞比例,实时定量PCR法检测富含嘌呤的核酸结合蛋白1(purine-rich nucleic acid binding protein 1,PU.1)和转录因子叉头框蛋白O1(forkhead box protein O1,Foxo1)mRNA表达。重组IL-7刺激脓毒性心肌病患者分选的Th9细胞后与自体CD8+T细胞、人脐静脉内皮细胞共培养,检测靶细胞死亡、毒性分子和细胞因子的分泌。组间比较采用Kruskal-Wallis检验或Dunns多重检验,刺激前后比较采用配对t检验或Wilcoxon配对检验。结果纳入2.例脓毒性心肌病患者、56例脓毒症患者、31例对照者。脓毒性心肌病患者IL-7水平低于脓毒症患者和对照者[75.71(42.2.,135.59)pg/mL vs.118.47(60.18,171.73)pg/mL vs.168.42.105.41,2.2.30)pg/mL,P2.在脓毒性心肌病患者和脓毒症患者的水平高于对照者[96.70(56.76,147.04)pg/mL vs.69.75(43.19,111.2.)pg/mL vs.2..13(19.33,42.11)pg/mL,P2.+CD8+细胞比例在三组之间的差异无统计学意义(P=0.477)。脓毒性心肌病患者Th9比例和Foxo1 mRNA表达低于脓毒症患者和对照者(P<0.001),脓毒性心肌病和脓毒症患者Tc9比例、PU.1 mRNA表达和IL-9水平均低于对照组(P<0.001)。脓毒性心肌病患者PBMCs经IL-7刺激后,Th9比例、PU.1和Foxo1 mRNA表达、IL-9分泌均高于无IL-7刺激(P<0.05)。脓毒性心肌病患者分选的Th9细胞经IL-7刺激后可促进自体CD8+T细胞介导的靶细胞死亡升高(P<0.05),上清液中穿孔素、颗粒酶B和干扰素-γ水平亦升高(P<0.05)。结论IL-7可增强脓毒性心肌病患者Th9细胞活性。

关键词：脓毒性心肌病脓毒症白细胞介素辅助性T细胞

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Selection of dialysis methods for end-stage kidney disease patients with diabetes

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World Journal of Diabetes 2024年第9期15卷 1862-1873页

作者： Yao-Hua Hu Ya-Li Liu Ling-Fei Meng Yi-Xian zhang Wen-Peng Cui department of Nephrology The Second Hospital of Jilin UniversityChangchun 130041Jilin ProvinceChina

The increasing prevalence of diabetes has led to a growing population of endstage kidney disease(ESKD)patients with ***,kidney transplantation is the best treatment option for ESKD patients;however,it is limited by the lack of ***,dialysis has become the standard treatment for ESKD ***,the optimal dialysis method for diabetic ESKD patients remains *** patients with diabetes often present with complex conditions and numerous ***,these patients face a high risk of infection and technical failure,are more susceptible to malnutrition,have difficulty establishing vascular access,and experience more frequent blood sugar fluctuations than the general ***,this article reviews nine critical aspects:Survival rate,glucose metabolism disorder,infectious complications,cardiovascular events,residual renal function,quality of life,economic benefits,malnutrition,and volume *** study aims to assist clinicians in selecting individualized treatment methods by comparing the advantages and disadvantages of hemodialysis and peritoneal dialysis,thereby improving patients’quality of life and survival rates.

关键词： Diabetes End-stage kidney disease Hemodialysis Peritoneal dialysis Dialysis methods selection

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Isoform of Vascular Endothelial Cell Growth Inhibitor (VEGI72.2.1) Increases Interleukin-2.Production by Activation of T Lymphocytes

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Acta Biochimica et Biophysica Sinica 2006年第4期38卷 249-253页

作者： Jing-Juan YAO~1 Min zhang~1 Xiao-Hui MIAO~2.Ping ZHAO~1 Shi-Ying ZHU~1 Hui DING~1 Zhong-Tian QI~1 1 Key Laboratory for Medical Microbiology,PLA,department of Microbiology,Second Military Medical University,Shanghai 2.0433,{2. 2.department of Infectious Diseases,Changzheng Hospital,Second Military Medical University Shanghai 2.0433,{2. KeyLaboratoryforMedicalMicrobiology PLADepartmentofMicrobiologySecondMilitaryMedicalUniversityShanghai200433China departmentofInfectiousDiseases ChangzhengHospitalSecondMilitaryMedicalUniversityShanghai200433China

The objective of this study is to investigate the characteristics of the recombinant variant ofhuman vascular endothelial cell growth inhibitor,VEGI72.2.1,and compare its biological activities with that ofits prototype VEGI2.-174,The recombinant plasmid containing the variant VEGI72.2.1gene was constructedand expressed in Escherichia *** effects of the expressed VEGI72.2.1on cell proliferations were checkedin the human umbilical vein endothelial cell line and certain tumor cell lines （ECV304 and B 16）.The inhibitionof VEGI72.2.1on angiogenesis was detected in the chorioallantoic membrane of chick *** comparisonwith VEGI2.-174,the recombinant human VEGI72.2.1seems to have no effect on the proliferation of endothelialcells and the angiogenesis of the chorioallantoic membrane in *** enzyme-linked immunosorbent assay-based method was used for the measurement of interleukin-2.（IL-2. production by peripheral blood monocytes（PBMCs） treated with *** were pretreated with VEGI72.2.1（1.2.-12.50μg/ml） for 2. h invitro,and the IL-2.concentration in PBMC medium was increased from 354 pg/ml to 12.6 pg/*** can beconcluded that VEGI72.2.1is able to increase the level of human IL-2.production by the activation of *** from VEGI2.-174on anti-angiogenesis,VEGI72.2.1may serve as an anti-cancer factorthrough its activation of T lymphocytes.

关键词： vascular endothelial cell growth inhibitor biological activity interleukin-2

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一个假性醛固酮减少症Ⅱ型家系临床和基因分析并文献复习

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中华内分泌代谢杂志 2024年第6期40卷 521-524页

作者：丁立新张萍王桧冯丽关小花杨启媚朱丽徐文龙张新萍黎丽圆曹金娟宝鸡高新人民医院内分泌科陕西721000

收集1个假性醛固酮减少症Ⅱ型(pseudohypoaldosteronism type II,PHA2.家系患者临床资料、化验检查及基因突变结果。先证者,1岁7个月,发现血钾6.69 mmol/L(参考范围3.5~5.3 mmol/L,下同)、血压110/68 mmHg(90 mL·min^(-1)·(1.73 m^(2.... 详细信息

收集1个假性醛固酮减少症Ⅱ型(pseudohypoaldosteronism type II,PHA2.家系患者临床资料、化验检查及基因突变结果。先证者,1岁7个月,发现血钾6.69 mmol/L(参考范围3.5~5.3 mmol/L,下同)、血压110/68 mmHg(2.~2. mmol/L)、估算的肾小球滤过率12..5 mL·min^(-1)·(1.73 m^(2.)^(-1)[>90 mL·min^(-1)·(1.73 m^(2.)^(-1)]、血肾素浓度0.30μIU/mL(4.2.45.6μIU/mL);其母亲、外祖父也有肾功能正常情况下高血钾、高血压、高血氯、代谢性酸中毒、低肾素。3例患者基因结果显示,无赖氨酸激酶4(WNK4)基因第7号外显子存在1个杂合错义变异(c.1685A>G,p.E562.);氢氯噻嗪治疗均有效。通过文献复习,将本文E562.家系与其他WNK4变异型进行了比较,提示WNK4突变型的临床表型异质性。对于不明原因的高钾血症,尤其合并高血压,要警惕PHA2.尽早进行基因筛查,避免PHA2.诊误诊。

关键词：假性醛固酮减少症Ⅱ型 WNK4基因高钾血症高血压

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