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Pichia pastoris composition expressed aerolysin mutant of Aeromonas veronii as an oral vaccine evaluated in zebrafish(Danio rerio)

第十五届全国泌尿外科学术会议

作者： zhang kaiyan, xing jinchun, chen bin, chen shixin, zhou zhongquan, liu rongfu, (Department of Urology, XiaMen First Hospital, Affiliated to Fujian Medical University, Xiamen , China 361003)

Objective: Although the stone-free rate for PCNL in treatment of renal stone is quite high, usually more than 90%, the patients might have residual stone, especially for patients with staghorn stone. These stone fragm...

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Marine Life Science & Technology 2024年第3期6卷 475-487页

作者： Yuan-Yuan Yao Qing-Shuang zhang Shu-bin liu Hong-Wei Yang xing-Yu chen Ya-Lin Yang chen-chen Gao Chao Ran Tsegay Teame Zhen zhang Zhi-Gang zhou Key Laboratory for Feed Biotechnology of the Ministry of Agriculture Institute of Feed Research of Chinese Academy of Agricultural SciencesBeijing 100081China Sino-Norway Fish Gastrointestinal Microbiota Joint Lab Institute of Feed Research of Chinese Academy of Agricultural SciencesBeijing 100081China Institute of Marine Sciences Shantou UniversityShantou 515063China Tigray Agricultural Research Institute(TARI)Mekelle Center Tigray 7101Ethiopia

Vaccines are one of the most practical means to stop the spreading of Aeromonas veronii in *** this study,virulence factor aerolysin mutant NTaer which has lost its hemolytic activity was used as a target *** pastoris constitutive secretory expression NTaer(GS115-NTaer)was used as a potential safe oral vaccine to evaluate its effectiveness on zebrafish *** result shows that vaccination of GS115-NTaer for four weeks did not affect the growth performance of the host,while eliciting an effective immune protective *** with the control group,the GS115-NTaer could significantly up-regulate the relative expression level of the intestinal tight junction protein 1α(TJP1α)gene,and significantly increased the contents of lysozyme(LYZ),complement C3 and C4 in the gut,indicating that the innate immune response of the fish was *** relative gene expression levels of macrophage-expressed gene 1(MPEG1)and T cell receptor(TCR-α)in the gut,and MPEG1,CD4,CD8,TCR-α,GATA3,and T-bet in the spleen were all increased significantly,indicating that the cellular immune response of the fish was ***,the contents of serum IgM and intestinal mucosa IgZ antibodies were significantly increased,which showed that humoral immunity was also ***,inoculation with GS115-NTaer significantly changed the structure of gut *** particular,the relative ratio of(Firmicutes+Fusobacteriota+Bacteroidota)/Proteobacteria was significantly higher than that of the control and GS115 ***,the vaccinated fish were challenged with ***,and the relative percent survival of GS115 and the GS115-NTear groups was 14.28%and 33.43%.This improvement of immunity was not only due to the specific immune response but also attributed to the improvement of innate immunity and the gut microbiota which was demonstrated by the germ-free zebrafish ***,this study provides information on the effectiveness of GS115-NTear

关键词： Aeromonas veronii Aerolysin mutant NTaer Oral vaccine Immune response Gut microbiota

Vibration-assisted material damage mechanism:From indentation cracks to scratch cracks

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Chinese Journal of Aeronautics 2024年第8期37卷 527-546页

作者： bingrui LV bin LIN Tianyi SUI Chunyan liu Jinshuo zhang Longfei WANG Xuhui chen Jingguo zhou Key Laboratory of Advanced Ceramics and Machining Technology Ministry of EducationTianjin UniversityTianjin 300072China Key Laboratory of Mechanism Theory and Equipment Design of Ministry of Education Tianjin UniversityTianjin 300072China Science and Technology of Advanced Functional Composite Laboratory Aerospace Research Institute of Materials and Processing TechnologyBeijing 100076China

Vibration-assisted grinding is one of the most promising technologies for manufacturing optical components due to its efficiency and quality ***,the damage and crack propagation mechanisms of materials in vibration-assisted grinding are not well *** order to elucidate the mechanism of abrasive scratching during vibration-assisted grinding,a kinematic model of vibration scratching was *** influence of process parameters on the evolution of vibration scratches to indentation or straight scratches is revealed by displacement metrics and velocity ***,scratch and vibration scratch experiments were performed on quartz glass,and the results showed that the vibration scratch cracks are a combination of indentation cracks and scratch *** scratch cracks change from indentation cracks to scratch cracks as the indenter moves from the entrance to the exit of the workpiece or as the vibration frequency changes from high to low.A vertical vibration scratch stress field model is established for the first time,which reveals that the maximum principal stress and tensile stress distribution is the fundamental cause for inducing the transformation of the vibration scratch cracking *** model provides a theoretical basis for understanding of the mechanism of material damage and crack propagation during vibration-assisted grinding.

关键词： Vibration assisted grinding Indentation Vibration scratch Material removal mechanism Stress field model Crack propagation mechanism

Observer-Based Adaptive Robust Precision Motion Control of a Multi-Joint Hydraulic Manipulator

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IEEE/CAA Journal of Automatica Sinica 2024年第5期11卷 1213-1226页

作者： Zheng chen Shizhao zhou Chong Shen Litong Lyu Junhui zhang bin Yao State Key Laboratory of Fluid Power and Mechatronic Systems Zhejiang UniversityHangzhou 310027 Ocean College Zhejiang UniversityZhoushan 316021China School of Mechanical Engineering Shijiazhuang Tiedao UniversityShijiazhuang 050000 State Key Laboratory of Fluid Power and Mechatronic Systems Zhejiang UniversityHangzhou 310027China School of Mechanical Engineering Purdue UniversityWest LafayetteIN 47907 USA

Hydraulic manipulators are usually applied in heavy-load and harsh operation ***,when faced with a complex operation,the traditional proportional-integral-derivative(PID)control may not meet requirements for high control ***-based full-state-feedback control is an effective alternative,but the states of a hydraulic manipulator are not always available and reliable in practical applications,particularly the joint angular velocity *** that it is not suitable to obtain the velocity signal directly from differentiating of position measurement,the low-pass filtering is commonly used,but it will definitely restrict the closed-loop bandwidth of the whole *** avoid this problem and realize better control performance,this paper proposes a novel observerbased adaptive robust controller(obARC)for a multi-joint hydraulic manipulator subjected to both parametric uncertainties and the lack of accurate velocity ***,a nonlinear adaptive observer is first designed to handle the lack of velocity measurement with the consideration of parametric ***,the adaptive robust control is developed to compensate for the dynamic uncertainties,and the close-loop system robust stability is theoretically proved under the observation and control ***,comparative experiments are carried out to show that the designed controller can achieve a performance improvement over the traditional methods,specifically yielding better control accuracy owing to the closed-loop bandwidth breakthrough,which is limited by low-pass filtering in fullstate-feedback control.

关键词： handle Observer filtering

A target parameter estimation method via atom-reconstruction in radar mainlobe jamming

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Journal of Systems Engineering and Electronics 2024年第2期35卷 350-360页

作者： zhou Bilei liu Weijian LI Rongfeng chen Hui zhang Liang DU Qinglei LI binbin chen Hao Air Force Early Warning Academy Wuhan 430019China Aerospace Nanhu Electronic Information Technology Co. Ltd.Jingzhou 434000China

Mainlobe jamming(MLJ)brings a big challenge for radar target detection,tracking,and *** suppression of MLJ is a hard task and an open problem in the electronic counter-counter measures(ECCM)*** parameters and target direction estimation is difficult in radar MLJ.A target parameter estimation method via atom-reconstruction in radar MLJ is proposed in this *** proposed method can suppress the MLJ and simultaneously provide high estimation accuracy of target range and ***,the eigen-projection matrix processing(EMP)algorithm is adopted to suppress the MLJ,and the target range is estimated effectively through the beamforming and pulse *** the target angle can be effectively estimated by the atom-reconstruction *** any prior knowledge,the MLJ can be canceled,and the angle estimation accuracy is well ***,the proposed method does not have strict requirement for radar array construction,and it can be applied for linear array and planar ***,the proposed method can effectively estimate the target azimuth and elevation simultaneously when the target azimuth(or elevation)equals to the jamming azimuth(or elevation),because the MLJ is suppressed in spatial plane dimension.

关键词： mainlobe jamming anti-jamming atom-reconstruction radar

Nonlinear empirical failure criterion for rocks under triaxial compression

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International Journal of Mining Science and Technology 2024年第3期34卷 351-369页

作者： Hongtao liu zhou Han Zijun Han Zihan chen Qinyu liu Hongkai zhang Rongguang zhang Linfeng Guo School of Energy&Mining Engineering China University of Mining and Technology-BeijingBeijing 100083China China National Coal Association Beijing 100010China

Based on existing triaxial compression experimental data,a new empirical failure criterion with wide applicability was proposed considering hydrostatic pressure,second stress invariance,and maximum shear *** fitting evaluation indicators were used to verify the consistency of the new failure criterion,and the differences with the other 6 failure criteria were *** characteristics of the new failure criteria in the principal stress space were finally *** results indicate that(1)the new failure criterion exhibits strong predictive ability for triaxial experiments and has good applicability for both intact and jointed rocks;(2)the influence of hydrostatic pressure on the failure surface exhibits a non-linear trend,and different hydrostatic pressure also exhibits different distribution patterns on the deviatoric stress plane,with a distribution characteristic pattern of hexagonal snowflake-regular *** maximum shear stress has a torsional effect on the new criterion,in the three-dimensional failure *** parameters a and b of the rock have an impact on the failure surface morphology of the new criterion function on the offset surface.

关键词： surface. criterion hydrostatic

ROS Balance Autoregulating Core-Shell CeO_(2)@ZIF-8/Au Nanoplatform for Wound Repair

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Nano-Micro Letters 2024年第8期16卷 341-361页

作者： Xi zhou Quan zhou Zhaozhi He Yi Xiao Yan liu Zhuohang Huang Yaoji Sun Jiawei Wang Zhengdong Zhao Xiaozhou liu bin zhou Lei Ren Yu Sun Zhiwei chen xingcai zhang The Higher Educational Key Laboratory for Biomedical Engineering of Fujian Province Research Center of Biomedical Engineering of XiamenDepartment of BiomaterialsCollege of MaterialsXiamen UniversityXiamen 361005People’s Republic of China Department of Electronic Science Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance ResearchSchool of Electronic Science and EngineeringXiamen UniversityXiamen 361005People’s Republic of China John A Paulson School of Engineering and Applied Sciences Harvard UniversityCambridgeMA 02138USA Department of Otorhinolaryngology Union Hospital of Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022People’s Republic of China NO.1 Middle School Affiliated to Central China Normal University Wuhan 430223People’s Republic of China

Reactive oxygen species(ROS)plays important roles in living *** ROS is a double-edged sword,which can eliminate drug-resistant bacteria,but excessive levels can cause oxidative damage to cells.A core–shell nanozyme,Ce O_(2)@ZIF-8/Au,has been crafted,spontaneously activating both ROS generating and scavenging functions,achieving the multifaceted functions of eliminating bacteria,reducing inflammation,and promoting wound *** Au Nanoparticles(NPs)on the shell exhibit high-efficiency peroxidase-like activity,producing ROS to kill ***,the encapsulation of Ce O_(2) core within ZIF-8 provides a seal for temporarily limiting the superoxide dismutase and catalase-like activities of Ce O_(2) ***,as the ZIF-8 structure decomposes in the acidic microenvironment,the Ce O_(2) core is gradually released,exerting its ROS scavenging activity to eliminate excess ROS produced by the Au *** two functions automatically and continuously regulate the balance of ROS levels,ultimately achieving the function of killing bacteria,reducing inflammation,and promoting wound *** innovative ROS spontaneous regulators hold immense potential for revolutionizing the field of antibacterial agents and therapies.

关键词： Metal-organic framework(MOF) Reactive oxygen species(ROS) Cerium dioxide Au nanoparticles Wound healing

Hemodialysis bilayer bionic blood vessels developed by the mechanical stimulation of hepatitis B viral X(HBX)genetransfected hepatic stellate cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第6期25卷 499-512页

作者： Hongyi liu Yuanyuan zhou Peng GUO Xiongwei ZHENG Weibin chen Shichao zhang Yu FU Xu zhou Zheng WAN bin ZHAO Yilin ZHAO Department of Oncology and Vascular Interventional Radiology Zhongshan Hospital Affiliated to Xiamen UniversitySchool of MedicineXiamen UniversityXiamen 361004China School of Medicine Xiamen UniversityXiamen 361102China Xiamen Health and Medical Big Data Center Xiamen 361008China Fujian Provincial Key Laboratory of Chronic Liver Disease and Hepatocellular Carcinoma(Zhongshan Hospital Affiliated to Xiamen University) Xiamen 361004China Xiamen Key Laboratory of Cellular Intervention and Interventional Medical Materials Xiamen 361004China

Artificial vascular graft(AVG)fistula is widely used for hemodialysis treatment in patients with renal ***,it has poor elasticity and compliance,leading to stenosis and *** ideal artificial blood vessel for dialysis should replicate the structure and components of a real artery,which is primarily maintained by collagen in the extracellular matrix(ECM)of arterial *** have revealed that in hepatitis B virus(HBV)-induced liver fibrosis,hepatic stellate cells(HSCs)become hyperactive and produce excessive ECM ***,mechanical stimulation can encourage ECM secretion and remodeling of a fiber *** on the above factors,we transfected HSCs with the hepatitis B viral X(HBX)gene for simulating the process of HBV ***,these HBX-HSCs were implanted into a polycaprolactonepolyurethane(PCL-PU)bilayer scaffold in which the inner layer is dense and the outer layer consists of pores,which was mechanically stimulated to promote the secretion of collagen nanofiber from the HBX-HSCs and to facilitate crosslinking with the *** obtained an ECM-PCL-PU composite bionic blood vessel that could act as access for dialysis after ***,the vessel scaffold was implanted into a rabbit’s neck arteriovenous fistula *** exhibited strong tensile strength and smooth blood flow and formed autologous blood vessels in the rabbit’s *** study demonstrates the use of human cells to create biomimetic dialysis blood vessels,providing a novel approach for creating clinical vascular access for dialysis.

关键词： Composite bilayer bionic blood vessel Extracellular matrix(ECM) Hepatic stellate cells(HSCs) Hepatitis B viral X(HBX)gene Mechanical force

Numerical multi-physical optimization of operating condition and current collecting setup for large-area solid oxide fuel cells

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Frontiers in Energy 2024年第3期18卷 356-368页

作者： chengrong YU Zehua PAN Hongying zhang bin chen Wanbing GUAN bin MIAO Siew Hwa CHAN Zheng ZHONG Yexin zhou School of Science Harbin Institute of TechnologyShenzhen 518055China Guangdong Provincial Key Laboratory of Deep Earth Sciences and Geothermal Energy Exploitation and Utilization Institute of Deep Earth Sciences and Green EnergyShenzhen UniversityShenzhen 518060China Key Laboratory of Advanced Fuel Cells and Electrolyzers Technology of Zhejiang Province Ningbo Institute of Materials Technology and Engineering of the Chinese Academy of SciencesNingbo 315201China Energy Research Institute at NTU(ERIAN) Nanyang Technological UniversitySingapore 639798Singapore

Due to the depletion of traditional fossil fuels and the aggravation of related environmental problems,hydrogen energy is gaining more attention all over the *** oxide fuel cell(SOFC)is a promising power generation technology operating on hydrogen with a high *** further boost the power output of a single cell and thus a single stack,increasing the cell area is an effective ***,it was recently found that further increasing the effective area of an SOFC single cell with a flat-tubular structure and symmetric double-sided cathodes would result in a lower areal *** this work,a multi-physical model is built to study the effect of the effective area on the cell *** distribution of different physical fields is systematically *** of the cell performance is also pursued by systematically tuning the cell operating condition and the current collection *** improvement of 42%is revealed by modifying the inlet gas flow rates and by enhancing the current *** the future,optimization of cell geometry will be performed to improve the homogeneity of different physical fields and thus to improve the stability of the cell.

关键词： solid oxide fuel cell(SOFC) large effective area flow rate discharge performance current collection