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Characterization of Gh SERK2.and its expression associated with somatic embryogenesis and hormones level in Upland cotton

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Journal of Integrative Agriculture 2018年第3期17卷 517-529页

作者： LIU zheng-jie ZHAO Yan-peng ZENG Ling-he {1. Yuan WANG Yu-mei {2. Jin-ping College of Agronomy and Biotechnology China Agricultural University/Key Laboratory of Crop Heterosis and Utilization Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement United States Department of Agriculture - Agricultural research Service (USDA-ARS) Crop Genetics Research Unit research {1. of Cash Crop Hubei Academy of Agricultural Sciences

Somatic embryogenesis （SE） is one of the most important steps during regeneration of cotton, but the molecular mechanism of SE remains unclear. SOMATIC EMBRYOGENSIS RECEPTOR KINASE （SERK） gene is known to function in SE. A homolog GhSERK2.（accession number： JF430801. was cloned from Upland cotton and characterized for its functions in SE. GhSERK2.expressed in different tissues and showed higher expression level in floral organs than vegetative ones with the highest levels in ovule and anther. GhSERK2.expressed during SE with a high level at globular embryos stage. Upon treatment with indole-3-butytic acid （IBA）, the transcription level of GhSERK2.was induced and promoted SE subsequently. A 2.day treatment of 2.4-dichlorophenoxyacetic acid （2.4-D） induced the expression of GhSERK2. but treatments of 2.4-D for longer periods sharply inhibited the GhSERK2.transcription level of embryogenic callus （EC）. The levels of hormones, including 3-indoleacetic acid （IAA）, abscisic acid （ABA）, and brassinosteroid （BR）, were increased in the initial calli induced from the over-expression of GhSERK2.cotton. Our results indicated that GhSERK2.expression was associated with induction of SE and closely related to hormone levels during tissue culture in Upland cotton, and the gene might play an important role in regeneration of cotton.

关键词： GhSERK2 somatic embryogenesis hormone level expression pattern Upland cotton (Gossypium hirsutum L.)

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Body Mass Index and Hypertension Hemodynamic Subtypes in Yi Farmers and Migrants

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Biomedical and Environmental sciences 2012年第1期25卷 53-60页

作者： SHAN Guang Liang wei Da Ying WANG Chun Xiu {1. Jian {2. WANG Bin MA Ming Ju PAN Li YU Tao XUE Fang WANG Ping WU zheng Lai Department of Epidemiology and Statistics＇ Institute of Basic Medical Sciences Chinese Academy of Medical Sciences School of Basic Medicine Peking Union Medical College Beijing 100005 China Liangshan Yi Autonomous Prefectural Center for Disease Control and Prevention Xichang 615000 Sichuan China Department of Epidemiology and Social Medicine Xuanwu Hospital Capital University of Medical Sciences Beijing 100053 China

Objective To examine the relationship between overweight or obesity and the risk of the various hypertension hemodynamic subtypes in Yi farmers and migrants. Methods A cross-sectional study of {1.358 Yi farmers and {1.39{1.Yi migrants was carried out in the Liangshan Yi autonomous prefecture, Sichuan,{{1. in {1.07. Results The standardized prevalence of overweight in female Yi farmers （6.{1.%） was higher than in males （3.{1.%）, whereas in Yi migrants 3{1.56% of males and {1..78% of females were overweight. The standardized prevalence of obesity was 0 and 0.6{1. in male and female Yi farmers, compared to 3.91. and 5.57% in male and female Yi migrants, respectively. For both genders the standardized prevalence of ISH, IDH, and SDH was higher in Yi migrants than Yi farmers. Overweight and obese Yi men and women had a higher risk for IDH and SDH （P〈O.00{1. than non-overweight/obese individuals. However, an association of overweight or obesity with ISH was observed only in men. Conclusion Yi migrants have substantially higher proportion of overweight and obese individuals, as well as individuals affected by ISH, IDH, and SDH, than do Yi farmers. Overweight and obesity are significant risk factors for the development of hypertension in Yi people.

关键词： Body mass index (BMI) Overweight Obesity Hypertension Ethnic Yi Migrant china

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Developmental stage-specific A-to-I editing pattern in the postnatal pineal gland of pigs(Sus scrofa)

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Journal of Animal Science and Biotechnology 2021年第1期12卷 198-206页

作者： Rong Zhou Wenye Yao Chundi Xie Leixia zhang Yangli Pei {1. Li {2. Feng Yalan Yang Kui Li Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding Key Laboratory of Animal Molecular Design and Precise Breeding of Guangdong Higher Education InstitutesSchool of Life Science and EngineeringFoshan UniversityFoshan 528231GuangdongChina State Key Laboratory of Animal Nutrition Key Laboratory of Animal Genetics Breeding and ReproductionMinistry of Agriculture and Rural AffairsInstitute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193China.

Background: RNA editing is a widespread post-transcriptional modification mechanism in mammalian *** many editing sites have been identified in domestic pigs(Sus scrofa), little is known about the characteristics and dynamic regulation of RNA editing in the pineal gland(PG), a small neuroendocrine gland that synthesizes and secretes melatonin, which is primarily responsible to modulate sleep ***: This study analyzed the expression of adenosine-to-inosine(A-to-I) editing regulators and profiled the first dynamic A-to-I RNA editome during postnatal PG development. The results identified ADAR1.as the most abundantly expressed ADAR enzyme, which was down-regulated during postnatal PG development. Furthermore,47,2.4 high-confidence RNA editing sites were identified, the majority of which(93.6%) were of the canonical A-to-I editing type, followed by C-to-T editing. Analysis of its characteristics showed that the A-to-I editing sites mostly localized in SINE retrotransposons PRE-1.Pre0_SS. Moreover, a strong deficiency and preference for guanine nucleotides at positions of one base upstream or downstream were found, respectively. The overall editing level at the puberty stage was higher than at both infancy and adulthood stages. Additionally, genome-wide RNA editing was found to exhibit a dynamic stage-specific fashion(postnatally). Genes that underwent developmental changes in RNA editing were associated with catabolic processes as well as protein localization and transport functions,implying that RNA editing might be responsible for the molecular machineries of the postnatal developing ***, RNA editing in 3′-UTRs might regulate gene expression by influencing miRNA binding during PG ***: This study profiles the first comprehensive developmental RNA editome in the pig PG, which contributes to the understanding of the importance of post-transcriptionally mediated regulation during mammalian postnatal PG development. Mo

关键词： A-to-I Pig Pineal gland Postnatal development RNA editing

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Layer-by-layer epitaxy of multi-layer MoS2.wafers

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National Science Review 2022年第6期9卷 153-162页

作者： Qinqin Wang Jian Tang Xiaomei Li Jinpeng Tian Jing Liang Na Li Depeng Ji Lede Xian Yutuo Guo Lu Li Qinghua {1. Yanbang Chu {2. wei Yanchong Zhao Luojun Du hua Yu Xuedong Bai Lin Gu Kaihui Liu wei Yang Rong Yang Dongxia Shi Guangyu {1. beijing National Laboratory for Condensed Matter Physics and {1. of Physics Chinese Academy of Sciences School of Physical sciences University of Chinese Academy of Sciences Collaborative Innovation Center of Quantum Matter and School of Physics Peking University Songshan Lake Materials Laboratory

The 2. semiconductor of MoS2.as great potential for advanced electronics technologies beyond *** far, high-quality monolayer MoS2.afers have been available and various demonstrations from individual transistors to integrated circuits have also been shown. In addition to the monolayer, multilayers have narrower band gaps but improved carrier mobilities and current capacities over the ***, achieving high-quality multi-layer MoS2.afers remains a challenge. Here we report the growth of high-quality multi-layer Mo S2.4-inch wafers via the layer-by-layer epitaxy process. The epitaxy leads to well-defined stacking orders between adjacent epitaxial layers and offers a delicate control of layer numbers up to six. Systematic evaluations on the atomic structures and electronic properties were carried out for achieved wafers with different layer numbers. Significant improvements in device performances were found in thicker-layer field-effect transistors(FETs), as expected. For example, the average field-effect mobility(μFE) at room temperature(RT) can increase from ～80 cm~2.V-1.s-1.or monolayers to ～1.0/1.5 cm~2.V-1.-1.or bilayer/trilayer devices. The highest RT μFEof 2.4.7 cm~2.V-1.s-1.nd record-high on-current densities of 1.70 m A·μm-1.t Vds= 2. were also achieved in trilayer MoS2.ETs with a high on/off ratio of >1.7. Our work hence moves a step closer to practical applications of 2. MoS2.n electronics.

关键词： 2. semiconductor multilayer MoS_2.wafer layer-by-layer epitaxy high performance transistors thin film transistors

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Genome-wide identification of agronomically important genes in outcrossing crops using OutcrossSeq

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Molecular Plant 2021年第4期14卷 556-570页

作者： Mengjiao Chen weijuan Fan Feiyang Ji {1. {1. Jie Liu Mengxiao Yan Qingguo Ma Jiongjiong Fan Qin Wang Shufeng zhang Guiling Liu Zhe Sun Changgeng Tian Fengling Zhao Jianli zheng Qi zhang Jiaxin Chen Jie Qiu Xin wei Ziru Chen Peng zhang Dong Pei Jun Yang Xuehui {1.ng Shanghai Key Laboratory of Plant Molecular sciences College of Life SciencesShanghai Normal UniversityShanghai 200234China Shanghai Key Laboratory of Plant Functional Genomics and Resources Shanghai Chenshan Plant Science Research CenterChinese Academy of SciencesShanghai Chenshan Botanical GardenShanghai 201602China State Key Laboratory of Tree Genetics and Breeding Key Laboratory of Tree Breeding and Cultivation of the State Forestry and Grassland AdministrationResearch Institute of ForestryChinese Academy of ForestryBeijing 100091China Tai'an academy of Agricultural {1. Tai'an 271000ShandongChina National Genomics Data Center Bio-Med Big Data CenterCAS Key Laboratory of Computational BiologyCAS-MPG Partner Institute for Computational BiologyShanghai Institute of Nutrition and HealthChinese Academy of SciencesShanghai 200031China CAS Center for Excellence of Molecular Plant sciences Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200233China These authors contributed equally to this article.

Many important crops(e.g.,tuber,root,and tree crops)are *** these crops,no inbred lines are available for genetic study and breeding because they are self-incompatible,clonally propagated,or have a long generation time,making the identification of agronomically important genes difficult,particularly in crops with a complex autopolyploid *** this study,we developed a method,OutcrossSeq,for mapping agronomically important loci in outcrossing crops based on whole-genome low-coverage resequencing of a large genetic population,and designed three computation algorithms in OutcrossSeq for different types of outcrossing *** applied OutcrossSeq to a tuberous root crop(sweet potato,autopolyploid),a tree crop(walnut tree,highly heterozygous diploid),and hybrid crops(double-cross populations)to generate high-density genotype maps for the outcrossing populations,which enable precise identification of genomic loci underlying important agronomic *** causative genes at these loci were detected based on functional *** together,our results indicate that OutcrossSeq is a robust and powerful method for identifying agronomically important genes in heterozygous species,including polyploids,in a cost-efficient *** OutcrossSeq software and its instruction manual are available for downloading at ***/tool/***.

关键词： genotyping genetic mapping outcrossing polyploid

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LSD1.控颗粒细胞自噬并抑制Wt1.与FSH调节的有腔卵泡形成

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Science Bulletin 2024年第8期69卷 1122-1136页

作者：朱子健何美娜张拓赵庭秦韶刚高萌王文基郑文影陈子琦刘龙萍郝明周波张华王建斌王凤超夏国良王超 State Key Laboratory of Farm Animal Biotech Breeding College of Biological SciencesChina Agricultural UniversityBeijing 100193China College of Basic Medicine Guizhou Medical UniversityGuiyang 550025China Guizhou Provincial Key Laboratory of Pathogenesis and Drug research on Common Chronic Diseases Department of PhysiologyCollege of Basic MedicineGuizhou Medical UniversityGuiyang 550025China School of Life sciences Taizhou UniversityTaizhou 318000China School of Life sciences Tsinghua UniversityBeijing 100084China Transgenic Animal Center National Institute of Biological SciencesBeijing 102206China Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western china College of Life ScienceNingxia UniversityYinchuan 750021China

In a growing follicle,the survival and maturation of the oocyte largely depend on support from somatic cells to facilitate FSH-induced mutual signaling and chemical *** apoptosis and autophagy in somatic cells are involved in the process of FSH-induced follicular development,the underlying mechanisms require substantial *** to our study,along with FSH-induced antral follicles(AFs)formation,both lysine-specific demethylase 1.LSD1.protein levels and autophagy increased simultaneously in granulosa cells(GCs)in a time-dependent manner,we therefore evaluated the importance of LSD upon facilitating the formation of AFs correlated to autophagy in *** knockout of Lsdl in GCs resulted in significantly decreased AF number and subfertility in females,accompanied by marked suppression of the autophagy in *** the one hand,depletion of Lsd1.resulted in accumulation of Wilms tumor 1.homolog(WT1.,at both the protein and mRNA ***1.prevented the expression of FSH receptor(Fshr)in GCs and thus reduced the responsiveness of the secondary follicles to FSH *** the other hand,depletion of LSD1.resulted in suppressed level of autophagy by upregulation of ATG1.L2.in *** finally approved that LSD1.contributed to these sequential activities in GCs through its H3K4me2.demethylase ***,the importance of LSD1.in GCs is attributable to its roles in both accelerating autophagy and suppressing WT1.expression to ensure the responsiveness of GCs to FSH during AFs formation.

关键词： LSD1 Autophagy H3K4me2 WT1 Follicle formation Follicle stimulating hormone

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Fluorescent competitive assay for melamine using dummy molecularly imprinted polymers as antibody mimics

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Journal of Integrative Agriculture 2016年第5期15卷 1166-1177页

作者： DU Xin-wei {1. Yan-xin SHE Yong-xin LIU Guang-yang ZHAO Feng-nian WANG Jing WANG Shan-shan JIN Fen SHAO {2. JIN Mao-jun zheng Lu-fei institute of Quality Standards and Testing Technology for Agro-Products Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China Key Laboratory of Agro-product Quality and Safety Beijing 100081 PR. China School of Chemical Engineering and Technology Harbin Institute of Technology Harbin 150001 P.R. China

A fluorescent competitive assay for melamine was first developed utilizing dummy molecularly imprinted polymers（DMIPs） as artificial antibodies. This method is based on the competition between fluorescent substances and the unlabeled analyte for binding sites in synthesized DMIPs and the decreased binding of fluorescent substances to DMIPs due to increased concentrations of melamine in the solutions. DMIPs for melamine were synthesized under a hot water bath in the presence of the initiator azobisisobutyronitrile（AIBN） using 2.4-diamino-6-methyl-1.3,5-triazine（DAMT） as a dummy template, methacrylic acid（MAA） as a functional monomer, and ethylene glycol dimethacrylate（EGDMA） as a crosslinking agent. The adsorption capacity and selectivity of DMIPs for melamine were evaluated by the isothermal adsorption curve and Scatchard analysis. The evaluation results showed that the synthesized DMIPs had specific recognition sites for melamine and the maximum adsorption amount was 1.066.33 μg g^（-1.. Later, 5-（4,6-d ichlorotriazinyl） amino fluorescein（DTAF） with a triazine ring, which s lightly resembles m elamine, w as selected as the fluorescent substance. The fluorescent competitive assay using DMIPs as t he antibody mimics was finally established by selecting and optimizing the reaction solvents, DMIPs amount, DTAF concentration, and incubation time. The optimal detection system showed a linear response w ithin range of 0.05-40 mg L^（-1. and the limit of detection（LOD） was 1.2. μg L^（-1.. It was successfully applied to the detection of melamine in spiked milk samples wi th satisfactory recoveries（71.9 to 86.3%）. According to the comparative analysis, the result of optimized fluorescent competitive assay re vealed excellent agreement with the HPLC-MS/MS result for melamine.

关键词： dummy molecularly imprinted polymers melamine fluorescent competitive assay artificial antibody

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Infrared Spectroscopy of CO2.Transformation by Group Ⅲ Metal Monoxide Cations

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chinese Journal of Chemical Physics 2020年第2期33卷 160-166,I0002,I0005-I0009页

作者： Dong Yang Ming-zhi Su Hui-jun zheng Zhi Zhao Xiang-tao Kong Gang Li {1. Xie {2.-qing zhang Hong-jun Fan Ling Jiang State Key Laboratory of Molecular Reaction Dynamics Collaborative Innovation Center of Chemistry for Energy and MaterialsDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China University of chinese academy of sciences Beijing 100049China

Infrared photodissociation spectroscopy of mass-selected[MO(CO2.n]^+(M=Sc,Y,La)complexes indicates that the conversion from the solvated structure into carbonate one can be achieved by the ScO^+ cation at n=5 and by the YO^+ cation at n=4,while only the solvated structures are observed for the LaO^+ *** findings suggest that both the ScO^+ and YO^+cations are able to fix CO2.into *** chemical calculations are performed on[MO(CO2.n]^+ to identify the structures of the low-lying isomers and to assign the observed spectral *** analyses show that the[YO(CO2.n]^+ complex has the smallest barrier for the conversion from the solvated structure into carbonate one,while[LaO(CO2.n]^+ exhibits the largest conversion barrier among the three metal oxide *** present system affords a model in clarifying the effect of different metals in catalytic CO2.transformation at the molecular level.

关键词： Infrared spectroscopy CO2.transformation Metal monoxide cation

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Fast synthesis of dopamine-coated Fe_3O_4 nanoparticles through ligand-exchange method

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chinese Chemical Letters 2012年第9期23卷 1099-1102页

作者： Peng An Fang Zuo Yuan Peng Wu Jun hua {1. Zhao Hui {2. Xiao Bin Ding Yu Xing Peng Chengdu institute of Organic Chemistry Chinese Academy of SciencesChina Chengdu 610041 Graduate School of the chinese academy of sciences China Beijing 100049 State Key Laboratory of Polymer Materials and Engineering of china Polymer Research InstituteSichuan UniversityChina Chengdu 610065 College of Chemistry&Environment Protection Engineering Southwest University for NationalitiesChina Chengdu 610041 State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation So0uthwest Petroleum University(SWPU)China Chengdu 610500

A fast approach was described for the synthesis of water-dispersible monodisperse dopamine-coated Fe304 nanoparticles （DA- Fe304） with uniform size and shape via ligand-exchange of oleic acid on Fe304 using only 2.min. The prepared DA-Fe304 nanoparticles were characterized by transmission electron microscopy, Fourier transform infrared spectrometry, and vibrating sample magnetometer. The results indicated that the resulting DA-Fe304 nanoparticles had an average diameter of about 1..2.nm. The magnetic saturation value of the prepared DA-Fe304 nanoparticles was determined to be 72.87 emu/g, which indicating a well- established superparamagnetic property.

关键词： Magnetic nanoparticles Fe304 Dopamine Ligand-exchange

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Identification of multi-target anti-cancer agents from TCM formula by in silico prediction and in vitro validation

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chinese Journal of {1. Medicines 2022年第5期20卷 332-351页

作者： zhang Bao-Yue {1. Yi-Fu ZHAO Jun KANG De WANG Zhe XU Lv-Jie LIU Ai-Lin DU Guan-{2. State Key Laboratory of Bioactive Substance and Function of natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China School of Life sciences Tsinghua UniversityBeijing 100084China

Cancer is a complex disease associated with multiple gene mutations and malignant phenotypes,and multi-target drugs provide a promising therapy idea for the treatment of *** products with abundant chemical structure types and rich pharmacological characteristics could be ideal sources for screening multi-target antineoplastic *** this paper,50 tumor-related targets were collected by searching the Therapeutic Target Database and Thomson Reuters Integrity database,and a multi-target anti-cancer prediction system based on mt-QSAR models was constructed by using naïve Bayesian and recursive partitioning algorithm for the first *** the multi-target anti-cancer prediction system,some dominant fragments that act on multiple tumor-related targets were analyzed,which could be helpful in designing multi-target anti-cancer ***-cancer traditional chinese medicine(TCM)and its {1. products were collected to form a TCM formula-based {1. products library,and the potential targets of the {1. products in the library were predicted by multi-target anti-cancer prediction *** a result,alkaloids,flavonoids and terpenoids were predicted to act on multiple tumor-related *** predicted targets of some representative compounds were verified according to literature review and most of the selected {1. compounds were found to exert certain anti-cancer activity in vitro biological *** conclusion,the multi-target anti-cancer prediction system is very effective and reliable,and it could be further used for elucidating the functional mechanism of anti-cancer TCM formula and screening for multi-target anti-cancer *** anti-cancer {1. compounds found in this paper will lay important information for further study.

关键词： Cancer Multi-target mt-QSAR model Nave Bayesian Recursive partitioning TCM formulae

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