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Dietary exposure to di-isobutyl phthalate increases urinary 5-methyl-2′-deoxycytidine level and affects reproductive function in adult male mice

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Journal of Environmental Sciences 2017年第11期29卷 14-23页

作者： Yitao Pan Xiaoyang Wang Leo W.Y.Yeung Nan Sheng Qianqian Cui Ruina Cui hongxia zhang Jiayin Dai Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China Man-Technology-Environment Research Centre (MTM) School of Science and Technology Orebro University Orebro SE -70182 Sweden

Phthalates are a large family of ubiquitous environmental pollutants suspected of being endocrine disruptors. Epidemiological studies have associated phthalate metabolites with decreased reproductive parameters and linked phthalate exposure with the level of urinary 5-methyl-2′-deoxycytidine（5mdC, a product of methylated DNA）. In this study, adult male mice were exposed to 450 mg di-isobutyl phthalate（DiBP）/（kg·day） via dietary exposure for 28 days. Mono-isobutyl phthalate（Mi BP, the urinary metabolite） and reproductive function parameters were determined. The levels of 5mdC and 5-hydroxymethyl-2′-deoxycytidine（5hmdC） were measured in urine to evaluate if their contents were also altered by DiBP exposure in this animal model. Results showed that DiBP exposure led to a significant increase in the urinary 5mdC level and significant decreases in sperm concentration and motility in the epididymis, accompanied with reduced testosterone levels and downregulation of the P450 cholesterol side-chain cleavage enzyme（P450scc） gene in the mice testes. Our findings indicated that exposure to DiBP increased the urinary 5mdC levels,which supported our recent epidemiological study about the associations of urinary 5mdC with phthalate exposure in the male human population. In addition, DiBP exposure impaired male reproductive function, possibly by disturbing testosterone levels; P450scc might be a major steroidogenic enzyme targeted by DiBP or other phthalates.

关键词： Di-isobutyl phthalate(DiBP) 'Reproductive function' 5-Methyl-2′-deoxycytidine(5mdC) 'Testosterone 'Deoxyribonucleic acid(DNA) methylation

分析右佐匹克隆联合重复经颅磁刺激对脑卒中后焦虑症伴睡眠障碍患者心理状态及睡眠质量的影响

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世界睡眠医学杂志 2024年第4期11卷 717-720页

作者：马福兴孔传祥张红霞侯琴霞青海省西宁市第二人民医院西宁810001

目的:分析右佐匹克隆联合重复经颅磁刺激对脑卒中后焦虑症伴睡眠障碍患者心理状态及睡眠质量的影响。方法:选取2021年1月至2022年12月青海省西宁市第二人民医院收治的脑卒中后焦虑症伴睡眠障碍患者100例作为研究对象,按照随机数字表法... 详细信息

目的:分析右佐匹克隆联合重复经颅磁刺激对脑卒中后焦虑症伴睡眠障碍患者心理状态及睡眠质量的影响。方法:选取2021年1月至2022年12月青海省西宁市第二人民医院收治的脑卒中后焦虑症伴睡眠障碍患者100例作为研究对象,按照随机数字表法分为观察组和对照组,每组50例。采用焦虑自评量表(SAS)比较2组患者干预前后焦虑状态的改善效果,采用睡眠状况自评量表(SRSS)评估2组脑卒中后焦虑症伴睡眠障碍患者的睡眠质量变化;治疗前后,采取多导睡眠监测仪对2组患者进行监测,观察睡眠质量,并比较2组患者的临床疗效差异。结果:干预后,观察组的有效率显著高于对照组,观察组SAS评分均显著低于对照组,观察组睡眠潜伏期、觉醒次数、睡眠规律均显著低于对照组,观察组睡眠时间、睡眠效率评分均显著高于对照组,SRSS量表中,观察组睡不安稳、很难入睡、多梦易醒、睡眠时间较短、提前醒来、觉醒不足、依赖安眠药、睡眠质量、白天状态和睡眠规律评分均显著低于对照组,差异均有统计学意义(均P<0.05)。结论:右佐匹克隆联合重复经颅磁刺激能有效改善脑卒中后焦虑症伴睡眠障碍患者的心理状态及睡眠质量,改善患者的焦虑情绪,值得临床推广使用。

关键词：右佐匹克隆重复经颅磁刺激脑卒中焦虑症睡眠障碍心理状态睡眠质量疗效

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Neuroprotective effect of high-dose hyperbaric oxygenation on rats with acute cerebral infarction in super-early stage:Curative comparison between 9-hour and 18-hour therapeutic protocols

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Neural Regeneration Research 2007年第11期2卷 649-654页

作者： Lianbi Xue Yongjun Wang Qiuhong Yu hongxia zhang Chunjuan Wang Yaling Liu Department of Hyperbaric Oxygenation Beijing Tiantan Hospital Capital Medical University Beijing 100050 China Department of Neurology Beijing Tiantan Hospital Capital Medical University Beijing 100050 China

BACKGROUND： Previously, only single short-time low-dose hyperbaric oxygenation （HBO） protocol was administrated to treat acute ischemic stroke in early stage and the conflicting results were obtained. There are few studies to report the outcome of administering long-time （can cover all the natural pathologic progression period） high-dose HBO to treat the disease. OBJECTIVE： To evaluate the therapeutic effect between two kinds of high-dose hyperbaric oxygenation on super-early stage of acute permanent middle cerebral artery occlusion （MCAO） in rats. DESIGN： A randomized controlled experimental study. SETTING： Beijing Tiantan Hospital, Capital Medical University; Beijing Research Institute of Neurosurgery. MATERIALS： Seventy-four male SD rats, aged 2.5 months old, weighing （ 280 ＋ 20） g, were provided by the Animal Institute, Chinese Academy of Medical Sciences. Hyperbaric oxygenation device was hyperbaric air cabin in which there was a self-made pure oxygen animal experimental cabin （made in China）. METHODS： This experiment was carried out in the municipal laboratory of Beijing Tiantan Hospital affiliated to Capital Medical University and Beijing Research Institute of Neurosurgery. ① Experimental intervention： All the rats were developed into models of permanent MCAO by suture embolism. Then, they were randomly divided into two HBO groups （9 hours and 18 hours） and control group, with 24 rats in each as well as 3-hour ultrastructure control group, with 2 rats. After being modeled for 3 hours, rats in the two HBO groups stayed in the hyperbaric cabin for 9 hours and 18 hours, separately. Rats in the 9-hour HBO group inhaled pure oxygen at hours 1, 3, 5, 7 and 9, and hyperbaric air at hours 2, 4, 6 and 8. Rats in the 18-hour HBO group inhaled pure oxygen at hours l, 3, 5, 7, 9, 11, 13, 15 and 17, and hyperbaric air at hours 2, 4, 6, 8, l0 12, 14, 16 and 18. After being created into models, rats in the control group and 3-hour ultrastructure control group breath

关键词： hyperbaric oxygenation middle cerebral artery occlusion neurobehavioral outcome infarct volume ultrastructure

石莼发酵条件优化及其在刺参养殖中的应用效果

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大连海洋大学学报 2024年第3期39卷 410-419页

作者：贾文广李慧丽潘晓静张洪霞于贞李蕊邢荣莲陈丽红烟台大学生命科学学院山东烟台264005

为优化石莼(Ulva lactuca)发酵饲料工艺并探索石莼发酵饲料对刺参(Apostichopus japonicus)生长、消化性能的影响,选取特基拉芽孢杆菌(Bacillus tequilensis)作为发酵剂,以发酵产物中还原糖、游离氨基酸和多肽为评价指标,采用单因素试... 详细信息

为优化石莼(Ulva lactuca)发酵饲料工艺并探索石莼发酵饲料对刺参(Apostichopus japonicus)生长、消化性能的影响,选取特基拉芽孢杆菌(Bacillus tequilensis)作为发酵剂,以发酵产物中还原糖、游离氨基酸和多肽为评价指标,采用单因素试验与响应面法对石莼发酵工艺条件进行优化,并进一步比较石莼发酵饲料、石莼未发酵干粉饲料和商品饲料对刺参生长性能、消化性能的影响。结果表明:石莼最佳发酵条件为温度33℃、接种菌量4.4×10^(6)CFU/g、含水率50%,此条件下发酵7 d,产物中游离氨基酸由3.6 mg/g增加到45.0 mg/g,多肽含量由30.1 mg/g增加到96.0 mg/g,还原糖含量由1.3 mg/g增加到20.8 mg/g;用3组饲料投喂刺参30 d后发现,刺参对3组饲料的摄食率无显著性差异(P>0.05),石莼发酵饲料组饲料转化率及消化酶活性均显著高于其他两个饲料组(P0.05),但显著高于石莼未发酵干粉饲料组(P<0.05)。研究表明,石莼发酵饲料可替代商品饲料,不仅能提高刺参增重率、消化酶活性,同时也为刺参饲料的配制提供了广泛的原料资源。

关键词：石莼发酵饲料工艺优化刺参养殖生长性能消化性能

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阿加曲班联合阿司匹林治疗急性脑梗死的临床获益

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中华保健医学杂志 2024年第4期26卷 431-434页

作者：田勇张洪霞李明航李东蛟郑建业沧州市中心医院神经外科 061000 沧州市中心医院肿瘤内科 061000

目的基于临床评分、血管内皮功能等指标探讨阿加曲班联合阿司匹林在急性脑梗死患者中的治疗作用。方法前瞻性选取沧州市中心医院2022年6月~2023年11月收治的108例急性脑梗死患者,按随机数表法将患者分为对照组和观察组,各54例,前者给... 详细信息

目的基于临床评分、血管内皮功能等指标探讨阿加曲班联合阿司匹林在急性脑梗死患者中的治疗作用。方法前瞻性选取沧州市中心医院2022年6月~2023年11月收治的108例急性脑梗死患者,按随机数表法将患者分为对照组和观察组,各54例,前者给予阿司匹林片单纯治疗,后者给予阿司匹林片联合阿加曲班注射液治疗,疗程均为1周。比较两组患者治疗前和治疗1周临床评分;比较两组患者治疗前和治疗1周血管内皮功能;比较两组患者治疗前和治疗1周脂蛋白相关磷脂A2(Lp-PLA2)、基质金属蛋白酶-9(MMP-9)、肿瘤坏死因子-α(TNF-α)等指标水平;比较两组患者治疗期间安全性。结果治疗1周后,观察组美国国立卫生研究院卒中量表(NIHSS)、改良Rankin量表(mRS)评分低于对照组,改良Barthel指数(MBI)高于对照组[(9.12±3.10)分vs.(16.30±2.92)分、(2.04±0.10)分vs.(4.45±0.37)分、(75.14±6.22)分vs.(60.90±5.77)分],差异均有统计学意义(t=12.389、46.206、12.334,P 0.05)。结论急性脑梗死患者应用阿加曲班联合阿司匹林治疗其症状明显改善,且安全性良好,其机制可能与调节血管内皮功能及血清细胞因子水平有关。

关键词：急性脑梗死阿加曲班阿司匹林血管内皮功能

Effects of Heavy Metal Ions (Cu^(2+), Pb^(2+) and Cd^(2+)) on DNA Damage of the Gills, Hemocytes and Hepatopancreas of Marine Crab, Charybdis japonica

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Journal of Ocean University of China 2011年第2期10卷 177-184页

作者： PAN Luqing LIU Na zhang hongxia WANG Jing MIAO Jingjing Department of Aquiculture the Key Laboratory of Mariculture Ministry of EducationOcean University of China Qingdao 266003 P. R. China

There are rising concerns about the hazardous effects of heavy metals on the environment. In this study, comet assay and DNA alkaline unwinding assay were conducted on the tissues (gills, hepatopancreas, and hemocytes) of Charybdis japonica in order to illustrate genotoxicity of three heavy metal ions (Cu2+, Pb2+, and Cd2+) on the marine crabs C. japonica. The crabs were exposed to Cu2+ (10, 50, and 100 ?g.L?1), Pb2+ (50, 250, and 500 ?g L?1) and Cd2+ (5, 25, and 50 ?g L?1), and the tissues were sampled at days 0.5, 1, 3, 6, 9, and 15. DNA alkaline unwinding assay was used for testing the DNA single strand break in gills and hepatopancreas and comet assay was employed for testing the DNA damage in hemocytes. The results showed that the DNA damage (F-value) of gills in the crabs exposed to the three heavy metals was decreased gradually during the exposure periods and there was a dose-time response relationship in certain time, suggesting that the levels of DNA single strand break in all the experimental groups increased significantly compared to the controls. Changes of F-value in hepatopancreas of the crabs exposed to the three heavy metals were similar to those in gills except that the peak values were found in the 500 ?g L?1 Pb2+ treatment group at day 3 and the 50 ?g L?1 Cd2+ treatment group at day 9. The ranks of DNA damage in gills and hepatopancreas induced by the three heavy metal ions (50 ?g L?1, day 15) were Cd2+ >Pb2+ >Cu2+ and Pb2+ >Cu2+ >Cd2+. The levels of DNA damage in gills were higher than those in hepatopancreas in the same experimental group. It can be concluded that indices of DNA damage can be used as the potential biomarkers of heavy metal pollution in marine environment.

关键词： crabs heavy metal genotoxicity comet assay DNA alkaline unwinding assay

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非言语学习障碍儿童中央执行系统缺陷

中国健康心理学杂志 2024年第6期32卷 836-842页

作者：史都苑张红霞肖泽梅胡春秀付瑶浙江师范大学儿童发展与教育学院杭州311231 厦门市同安区特殊教育学校杭州市钱塘区月雅河小学

目的:考察非言语学习障碍儿童的中央执行系统是否存在缺陷。方法:通过数字转换任务、数值-大小Stroop任务和字母活动记忆任务分别对非言语学习障碍儿童和典型发展儿童中央执行系统的3个子功能(转换、抑制、刷新)进行测量。结果:非言语... 详细信息

目的:考察非言语学习障碍儿童的中央执行系统是否存在缺陷。方法:通过数字转换任务、数值-大小Stroop任务和字母活动记忆任务分别对非言语学习障碍儿童和典型发展儿童中央执行系统的3个子功能(转换、抑制、刷新)进行测量。结果:非言语学习障碍儿童在数字转换任务(t_(30)=4.11,P<0.001)、数值-大小Stroop任务(t_(30)=2.87,P<0.01)和字母活动记忆任务(t_(30)=3.93,P<0.001)中的正确率都显著低于典型发展儿童,其中,在数字转换任务上的缺陷效应量(Cohens’d=1.47)最大。结论:非言语学习障碍儿童在中央执行系统的三类子功能上均存在不同程度的缺陷,其中转换功能是其核心缺陷。

关键词：非言语学习障碍儿童中央执行系统转换功能抑制功能刷新功能

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血脂异常与认知障碍的关系研究进展

全科护理 2024年第9期22卷 1650-1653页

作者：赵越段晓霞张洪霞邹玉婷程捷张代英西南医科大学护理学院 646000 西南医科大学附属医院 646000

对血脂异常与认知障碍进行概述并介绍二者关系及认知障碍发生机制、治疗,以期寻求改善认知障碍的有效干预措施,为认知障碍相关疾病的早期预防和治疗提供新思路。

关键词：血脂异常认知障碍认知功能他汀类药物地中海饮食综述

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Discrimination of pharmacodynamic components and volatile compounds of Poria cocos cultivated with five different materials based on GC-IMS analysis

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Food Quality and Safety 2024年第1期8卷 135-144页

作者： Yinan Wang Fei Zhou Hui Wang Jinping zhang hongxia Xu Research Institute of Subtropical Forestry Chinese Academy of ForestryHangzhouChina Chun‘an County Thousand-island Lake Forest Farm HangzhouChina

Objectives:The chemical constituents of Poria cocos grown with different substrates vary significantly;thus,identifying and comparing their biomarkers are *** and Methods:Herein,the chemical constituents of Poria cocos obtained with five different substrates were assessed using gas chromatography–ion mobility spectrometry(GC-IMS),high-performance liquid chromatography and multivariate statistical ***:The content of moisture,ash,alcohol-soluble matter,and heavy metals,except for those of the miscellaneous wood Poria cocos,conform to the specifications defined in the Chinese Pharmacopoeia(Edition 2020),and the polysaccharide contents are all greater than 57%.Conclusions:Based on GC-IMS and the established fingerprints,87 compounds were detected,70 of which were identified in each *** statistical analysis revealed seven compounds(two esters,three alcohols,and two aldehydes),which could be considered as potential marker compounds for discrimination.

关键词： Poria cocos cultivation material pharmacodynamic component gas chromatography-ion mobility spectrometry(GC-IMS) volatile compound.