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Adverse effects of metal exposure on chemotaxis towards water-soluble attractants regulated mainly by ASE sensory neuron in nematode Caenorhabditis elegans

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Journal of Environmental Sciences 2009年第12期21卷 1684-1694页

作者： XING Xiaojuan, DU Min, zhang Yanfen, WANG dayong Key Laboratory of Developmental Genes and Human Disease in Ministry of Education, Department of Genetics and Developmental Biology, Southeast University Medical School, Nanjing 210009, China. Key Laboratory of Developmental Genes and Human Disease in Ministry of Education Department of Genetics and Developmental Biology Southeast University Medical School Nanjing 210009 China

Chemotaxis to water-soluble attractants is mainly controlled by ASE sensory neuron whose specification is regulated by che-1 in Caenorhabditis elegans. Our data suggested that exposure to high concentrations of metals, such as Pb, Cu, Ag, and Cr, would result in severe defects of chemotaxis to water-soluble attractants of NaCl, cAMP, and biotin. Moreover, the morphology of ASE neuron structures as observed by relative fluorescent intensities and relative size of fluorescent puncta of cell bodies, relative lengths of sensory endings in ASE neurons, and the expression patterns of che-1 were obviously altered in metal exposed animals when they meanwhile exhibited obvious chemotaxis defects to water-soluble attractants. In addition, the dendrite morphology could be noticeably changed in animals exposed to 150 μmol/L of Pb, Cu, and Ag. Furthermore, we observed significant decreases of chemotaxis to water-soluble attractants in Pb exposed che-1 mutant at concentrations more than 2.5 μmol/L, and in Cu, Ag, and Cr exposed che-1 mutant at concentrations more than 50 μmol/L. Therefore, impairment of the ASE neuron structures and functions may largely contribute to the appearance of chemotaxis defects to water-soluble attractants in metal exposed nematodes.

关键词： chemotaxis water-soluble attractant ASE neuron che-1 Caenorhabditis elegans

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Light-Mediated Spatiotemporally Dynamic Assembly of DNA Nanostructures in Living Cells Regulates Autophagy

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CCS Chemistry 2024年第6期6卷 1557-1570页

作者： Rui zhang Pin Hu Yuwei Xu Ziqing Wang dayong Yang Chi Yao Frontiers Science Center for Synthetic Biology Key Laboratory of Systems Bioengineering(MOE)School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300350 Department of Chemistry State Key Laboratory of Molecular Engineering of PolymersShanghai Key Laboratory of Molecular Catalysis and Innovative MaterialsCollege of Chemistry and MaterialsFudan UniversityShanghai 200438

The assembly of exogenous artificial architectures inside cells can regulate a series of biological events,which heavily relies on the development of spatiotemporally controlled molecular assembly *** herein report a designer deoxyribonucleic acid(DNA)nanostructure that enables light-mediated spatiotemporally dynamic assembly in living cells and consequently achieves efficient regulation of cell *** DNA nanostructure was constructed from i-motif moiety-containing branched DNA,photocleavable bond-containing linker,and tumor cell-targeting *** cellular uptake mediated by aptamers,under the spatiotemporal control of both UV light and late endosomal/lysosomal acidic environments,disassembly/reassembly of DNA nanostructure occurred via two rationally designed routes,generating microsized DNA *** a result,autophagy was significantly enhanced with the increase of DNA assembly *** enhanced autophagy showed an impact on related biological *** system is expected to be a powerful tool for the regulation of intracellular events and cellular behaviors.

关键词： DNA nanotechnology DNA hydrogel self-assembly autophagy i-motif

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固化工艺对氰酸酯改性厌氧胶性能影响研究

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化学与粘合 2024年第4期46卷 340-344页

作者：胡明忠王刚刘晓辉朱金华米长虹张大勇荣立平黑龙江省科学院石油化学研究院黑龙江哈尔滨150040

以乙氧基化双酚A二甲基丙烯酸酯(EBDMA)作为厌氧胶单体,酚醛型氰酸酯(NCE)作为厌氧胶的耐热树脂改性剂,制备了氰酸酯改性的厌氧胶粘剂,探究了不同固化条件下改性厌氧胶粘接性能和热稳定性能,并借助DSC对室温固化样和180℃热处理固化样... 详细信息

以乙氧基化双酚A二甲基丙烯酸酯(EBDMA)作为厌氧胶单体,酚醛型氰酸酯(NCE)作为厌氧胶的耐热树脂改性剂,制备了氰酸酯改性的厌氧胶粘剂,探究了不同固化条件下改性厌氧胶粘接性能和热稳定性能,并借助DSC对室温固化样和180℃热处理固化样进行表征。结果表明,室温固化条件下,氰酸酯改性厌氧胶室温扭矩强度低于未改性厌氧胶;30份NCE改性厌氧胶180℃热强度的破坏扭矩和拆卸扭矩分别比未改性厌氧胶提高了123.15%和128.64%,10份NCE改性厌氧胶经180℃热处理后的最大扭矩提高了45.37%。通过TG分析,室温固化条件下,30份NCE改性厌氧胶的T_(d5%)达到329.21℃,比未改性厌氧胶提高了63.53℃。经180℃热处理后,100份NCE改性厌氧胶T_(d5%)为388.11℃,比未改性厌氧胶提高了85.74℃。DSC曲线数据表明,室温固化条件下,随着氰酸酯比例的增加,其固化程度逐渐降低;经180℃热处理后,固化程度增加,但仍未反应完全。氰酸酯的加入提高了厌氧胶的热稳定性能和粘接性能。

关键词：氰酸酯厌氧胶热失重固化工艺扭矩强度

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Ad Hoc网络中的地理路由机制

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计算机工程 2005年第5期31卷 143-145,174页

作者：张莉张连芳张大勇天津大学计算机科学与技术系天津300072 中兴通讯股份有限公司

介绍了各种AdHoc路由协议,讨论了AdHoc网络的地理路由机制。地理路由使用节点的地理位置作为它们的地址来将数据包传向目的节点。节点仅须知道邻节点的位置,而且不需要有明确的路由建立,它具有很好的扩展性并且支持节点间通信的任意性。

关键词：地理路由位置服务恢复策略

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Prokaryotic Expression of MBP and Its Effects on Phenotypical and Functional Maturation of Dendritic Cells

Prokaryotic Expression of MBP and Its Effects on Phenotypica...

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第十一届全国免疫学学术大会

作者： zhang dayong Weng Xiumei Wang Baoming Pan Jianping School of Medicine Zhejiang University City College

Background: Maltose binding protein(MBP) is a kind of immunoadjuvant and it can improve the immunogenicity of recombinant subunit vaccine. Recently studies showed that MBP had extensive immune activity which could activate NK cells, macrophages and Th1 cells, which indicated that MBP could be used as immunopotentiator which could improve the immune response to panthogens. Objectives: To construct the prokaryotic expression system of MBP. To examined the effects of MBP on the phenotypical and functional maturation of dendritic cells(DCs). Methods: Using genomicDNA of *** K12 as the template, PCR was performed to amplify entire mal E gene and then constructed the prokaryotic expression systems of mal E gene. Expression of MBP was detected by SDS-PAGE and the expressed MBP were extracted by Amylose Resin. Then the immunoreactivity of MBP was tested by Western Blot. DC2.4, a cell line of DC, was treated by the different concentration MBP. Then the phenotypic of DC2.4 was analyses by flow cytometry. The concentrations of IL-1b, IL-6, IL-12 and IL-23 in the culture supernatants were measured by ELISA. Results: The DNA sequences of the cloned mal E gene were completely identical with the reported corresponding gene. The constructed prokaryotic expression system could express MBP, and protein contents reached 0.2 mg/m L, MBP could be identified by Western Blot. MBP could promoted the expression of CD40, CD54, CD80, CD86 and MHC I on the surface of DCs, while inhibited the expression of MHC II. MBP stimulated the DCs to secreting IL-23, but did not affect the expression of IL-1β, IL-6 and IL-12. Conclusion: We construct the prokaryotic expression system of MBP and MBP is expressed and purified. MBP can in-duce the maturation of DCs and the production of proinflammatory cytokines.

关键词： MBP dendritic cells prokaryotic expression

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Screening of Transgenic Soybean Transformed by Means of Pollen-tube Using Kanamycin

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Journal of Northeast Agricultural University(English Edition) 2006年第1期13卷 7-10页

作者： zhang Shuzhen XU Pengfei zhang dayong LIN Shifeng LI Wenbin HAN Yingpeng YANG Chuanping Soybean Research Institute Northeast Agricultural University Harbin Heilongjiang 150030 PRC Forestry College Northeast Forestry University Harbin Heilongjiang 150040 PRC

Kanamycin was used to screen To seeds of the variety Dongnong 46 transformed by means of pollen-tube method. The results showed that 400 mg·L^-1 kanamycin could inhibit growth of non-transgenic plants, and 2 positive plants were gotten combined with Gus dyeing and PCR detection. It is proved that this method is economic and effective in preliminary screening the transgenic plants.

关键词： soybean pollen-tube method kanamycin

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社交媒体环境下用户信任度评估与传播影响力研究

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数据分析与知识发现 2018年第7期2卷 26-33页

作者：景东张大勇哈尔滨工业大学互动媒体设计与装备服务创新重点实验室哈尔滨150001

【目的】通过研究社交媒体用户信任度评估和传播影响力,发现推动或制约信息传播的关键因素,为促进社交媒体健康有序发展提供服务。【方法】根据网络信任特点,提出一种基于直接信任和间接信任的综合评估指标,该指标综合考虑个体的局部影... 详细信息

【目的】通过研究社交媒体用户信任度评估和传播影响力,发现推动或制约信息传播的关键因素,为促进社交媒体健康有序发展提供服务。【方法】根据网络信任特点,提出一种基于直接信任和间接信任的综合评估指标,该指标综合考虑个体的局部影响力和全局调控能力。【结果】SIR模型评估实验结果表明,综合评估指标值最大的个体发起的传播能在较短的传播时间内达到最大的范围。【局限】数据来源不够广泛,可能导致研究偏差。【结论】所构建的综合评估指标能够更为准确地度量网络中每个个体的信任水平。

关键词：社交媒体直接信任综合信任传播影响力

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The research of gain modulation laser imaging experiments

The research of gain modulation laser imaging experiments

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国防光电子论坛第二届激光雷达成像探测技术及应用研讨会

作者：屠志鹏 Li Sining zhang dayong Lu Wei Department of optoelectronic Harbin institute of technology

Gain modulation laser imaging radar is a major laser radar. By the control of the accurate gate door, the backscatter noise can be suppressed easily. It can make the image more accurately and obtain the distance information of the target. In contrast to the past, it’s different that it chose the nonlinear gain which was closer to the actual situation as the modulation gain. Afterwards, analyzed the echo energy, set up the SNR model and formed a set of theoretical algorithm. On the basis, the model was used to estimate the experimental distance with good imaging effect. Imaging under the condition of constant gain and modulation gain by experiments, used the images to restore the distance information and analyzed the imaging result. Finally, the distance precision can reach 0.5m.

关键词： Imaging system Gain modulation Nonlinear gain SNR

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加强“21世纪海上丝绸之路”战略支点建设研究

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中国工程科学 2016年第2期18卷 105-110页

作者：张大勇天津大学管理与经济学部天津300192

21世纪海上丝绸之路战略支点是指承载我国与沿线国家经贸、科技、文化合作功能的空间区域。近年来,我国与沿线国家在经贸合作园区、交通基础设施建设等方面开展了广泛合作,取得了良好效果,但也存在顶层设计不清晰、空间布局不合理、合... 详细信息

21世纪海上丝绸之路战略支点是指承载我国与沿线国家经贸、科技、文化合作功能的空间区域。近年来,我国与沿线国家在经贸合作园区、交通基础设施建设等方面开展了广泛合作,取得了良好效果,但也存在顶层设计不清晰、空间布局不合理、合作方式较单一、合作层次不丰富等问题。本研究以围绕"五通"加强战略支点建设为研究目标,在我国对外贸易数据分析为基础,利用层次分析法和德尔菲法,提出了战略支点建设的重点区域和建设模式,并从推进经贸合作区建设、加强科技文化交流、深化地区与行业合作等方面提出了政策建议。

关键词： 21世纪海上丝绸之路战略支点经贸合作科技合作

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原始成分组成对Ti(C,N)基金属陶瓷烧结性能的影响

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粉末冶金技术 2012年第2期30卷 83-88,97页

作者：张大勇石增敏天津瑞克曼德科技发展有限公司天津300050 三峡大学机械与材料学院湖北宜昌443002

采用真空烧结工艺制备了Ti(C,N)基金属陶瓷,研究了原始成分组成对材料烧结特性和力学性能的影响规律。结果表明:保持Mo/(Mo+Ni)为定值,随着硬质相成分的增加,烧结体抗弯强度降低,硬度增加;硬质相成分TiN含量超过一定阈值时,烧结体疏松... 详细信息

采用真空烧结工艺制备了Ti(C,N)基金属陶瓷,研究了原始成分组成对材料烧结特性和力学性能的影响规律。结果表明:保持Mo/(Mo+Ni)为定值,随着硬质相成分的增加,烧结体抗弯强度降低,硬度增加;硬质相成分TiN含量超过一定阈值时,烧结体疏松导致硬度和抗弯强度同时大幅下降;TiN含量保持定值,硬质相成分含量增加,烧结体硬度增加,抗弯强度下降;粘结相成分用Co部分替代Ni,随Co含量的增加,烧结体硬度略有升高的基础上抗弯强度升高。

关键词： Ti(C,N)基金属陶瓷真空烧结成分组成力学性能

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