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Bridging sciatic nerve gap using tissue-engineered nerves constructed with neural tissue-committed stem cells derived from bone marrow

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Neural Regeneration Research 2009年第5期4卷 344-349页

作者： Zhiying zhang Congli Ren chuansen zhang Fang Liu Liang Li Department of Anatomy Second Military Medical University of Chinese PLA Shanghai 200433 China

BACKGROUND： Schwann cells are the most commonly used cells for tissue-engineered nerves. However, autologous Schwann cells are of limited use in a clinical context, and allogeneic Schwann cells induce immunological rejections. Cells that do not induce immunological rejections and that are relatively easy to acquire are urgently needed for transplantation. OBJECTIVE： To bridge sciatic nerve defects using tissue engineered nerves constructed with neural tissue-committed stem cells （NTCSCs） derived from bone marrow; to observe morphology and function of rat nerves following bridging; to determine the effect of autologous nerve transplantation, which serves as the gold standard for evaluating efficacy of tissue-engineered nerves. DESIGN, TIME AND SETTING： This randomized, controlled, animal experiment was performed in the Anatomical Laboratory and Biomedical Institute of the Second Military Medical University of Chinese PLA between September 2004 and April 2006. MATERIALS： Five Sprague Dawley rats, aged 1 month and weighing 100-150 g, were used for cell culture. Sixty Sprague Dawley rats aged 3 months and weighing 220-250 g, were used to establish neurological defect models. Nestin, neuron-specific enolase （NSE）, glial fibrillary acidic protein （GFAP）, and S-100 antibodies were provided by Santa Cruz Biotechnology, Inc., USA. Acellular nerve grafts were derived from dogs. METHODS： All rats, each with 1-cm gap created in the right sciatic nerve, were randomly assigned to three groups. Each group comprised 20 rats. Autograft nerve transplantation group： the severed 1-cm length nerve segment was reverted, but with the two ends exchanged; the proximal segment was sutured to the distal sciatic nerve stump and the distal segment to the proximal stump. Blank nerve scaffold transplantation group： a 1-cm length acellular nerve graft was used to bridge the sciatic nerve gap. NTCSC engineered nerve transplantation group： a 1-cm length acellular nerve graft, in which NTCSCs were in

关键词： tissue-engineered nerve nerve damage sciatic nerve neural tissue-committed stem cells d

Role of neuregulin-1 in peripheral nerve injury

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Neural Regeneration Research 2010年第14期5卷 1104-1108页

作者： Zhengdong Kong Zhiying zhang Haiyan Lin chuansen zhang Department of Anatomy Second Military Medical University of Chinese PLA Shanghai 200433 China

Peripheral nerve injury in humans often leads to permanent functional deficits. Schwann cells play an important role in the recovery of peripheral nerve injury by ensheathing axons and providing various neurotrophic factors. Neuregulin-1 （NRG-1） provides axonal signals, which allow dedifferentiation and rapid proliferation of Schwann cells. Subsequently, NRG-1 promotes axonal myelination and influences myelin thickness. Moreover, NRG-1 plays a critical role in synapse formation in the neuromuscular junction. These effects, together, suggest that NRG-1 promotes recovery of peripheral nerve injury.

关键词： neuregulin-1 nerve injury neurotrophic factors neuroregeneration peripheral nerve Schwann cells myelination review

Combined transplantation of bone marrow mesenchymal stem cells and pedicled greater omentum promotes locomotor function and regeneration of axons after spinal cord injury in rats

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Neural Regeneration Research 2008年第4期3卷 358-362页

作者： Liang Li Zhiying zhang Haiyan Lin Congli Ren chuansen zhang Department of Anatomy the Secondary Military Medical University of Chinese PLA Shanghai 200433 China

BACKGROUND： According to previous studies, the neuroprotective effect of the pedicled greater omentum may be attributed to the secretion of neurotrophic factors and stimulation of angiogenesis. The neurotrophic factors released from the pedicled greater omentum, such as brain-derived neurotrophic factor and neurotrophin 3/4/5 could exert a neuroprotective effect on the damaged host neural and glial cells, and also could induce the transdifferentiation of transplanted bone marrow mesenchymal stem cells （BMSCs） into neural cells. OBJECTIVE： Based on the functions of the omentum of neuro-protection and vascularization, we hypothesize that the transplantation of BMSCs and pedicled greater omentum into injured rat spinal cord might improve the survival rate and neural differentiation of transplanted BMSCs and consequently gain a better functional outcome. DESIGN, TIME AND SETFING： A randomized, controlled animal experiment. The experiments were carried out at the Department of Anatomy, the Secondary Military Medical University of Chinese PLA between June 2005 and June 2007. MATERIALS： Fifteen male inbred Wistar rats, weighing （200±20） g, provided by the Experimental Animal Center of the Secondary Military Medical University of Chinese PLA were used and met the animal ethical standards. Mouse anti-BrdU and mouse anti-NF200 monoclonal antibody were purchased from Boster, China. METHODS： Cell culture： We used inbred Sprague-Dawley rats to harvest bone marrow for culture of BMSCs and transplantation to avoid possible immune rejection. BMSCs were cultured via total bone marrow adherence. Experimental grouping and intervention： The rats were randomly divided into a control group, cell group and combined group, five rats per group. Rats in the control group underwent spinal cord injury （SCI） only, during which an artery clamp with pressure force of 30 g was employed to compress the spinal cord at the Tl0 level for 30 seconds to produce the SCI model. 5 μ L PBS containing 10^5 B

关键词： spinal cord injuries transplantation mesenchymal stem cell omentum nerve regeneration

miR-124 and miR-128 differential expression in bone marrow stromal cells and spinal cord-derived neural stem cells

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Neural Regeneration Research 2010年第11期5卷 820-824页

作者： Chunfang Wang Hongen Wei chuansen zhang Pengfei Li Fei Wang Shufeng Han Department ofBiotechnology Shanxi Medical University Taiyuan 030001 Shanxi Province China Department of Anatomy Second Military Medical University Shanghai 200433 China Department of Orthopedics First Affiliated Hospital of Shanxi Medical University Taiyuan 030001 Shanxi Province China

BACKGROUND： MicroRNA （miRNA） expression in stem cells provides important clues for the molecular mechanisms of stem cell proliferation and differentiation. Bone marrow stromal cells and spinal cord-derived neural stem cells exhibit potential for neural regeneration. However, miRNA expression in these cells has been rarely reported. OBJECTIVE： To explore differential expression of two nervous system-specific miRNAs, miR-124 and miR-128, in bone marrow stromal cells and spinal cord-derived neural stem cells. DESIGN, TIME AND SETTING： An In vitro, cell biology experiment was performed at the Department of Biotechnology, Shanxi Medical University from June 2008 to June 2009. MATERIALS： TaqMan miRNA assays were purchased from Applied Biosystems. METHODS： Rat bone marrow stromal cells were isolated and cultured using the whole-bone marrow method, and rat spinal cord-derived neural stem cells were obtained through neurosphere formation. TaqMan miRNA assays were used to measure miR-124 and miR-128 expression in bone marrow stromal cells and spinal cord-derived neural stem cells. MAIN OUTCOME MEASURES： Morphology of bone marrow stromal cells and spinal cord-derived neural stem cells were observed by inverted microscopy. Expression of the neural stem cell-specific marker, nestin, the bone marrow stromal cell surface marker, CD71, and expression of miR-124 and miR-128, were detected by real-time polymerase chain reaction. RESULTS： Cultured bone marrow stromal cells displayed a short fusiform shape. Flow cytometry revealed a large number of CD71-positive cells （〉 95%）. Cultured spinal cord-derived neural stem cells formed nestin-positive neurospheres, and quantitative detection of miRNA demonstrated that less miR-124 and miR-128 was expressed in bone marrow stromal cells compared to spinal cord-derived neural stem cells （P 〈 0.05）. CONCLUSION： Bone marrow stromal cells and spinal cord-derived neural stem cells exhibited differential expression of miR-124 and miR-128, which sugg

关键词： bone marrow stromal cells spinal cord-derived neural stem cells miR-124 miR-128 spinal cord injury

Inhibitory effect of electro-acupuncture on apoptosis after spinal cord injury

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Neural Regeneration Research 2006年第3期1卷 239-243页

作者： Zhiying zhang Ruishan Dang chuansen zhang Liang Li Congli Ren Shuang Zhou Eryu Chen Department of Anatomy Second Military Medical University of Chinese PLA Shanghai 200433 China Shanghai Research Center of Acupuncture ＆ Mendian Shanghai 201203 China Department of Acupuncture and Moxibustion Second Military Medical University of Chinese PLA Shanghai 200433. China

BACKGROUND： Acupuncture and electro-acupundura （EA） as complementary and alternative medicine have been applied for neurological recovery after spinal cord injury （SCI）, but the mechanism of treatment is unclear yet. OBJECTIVE： To examine and compare the effects of EA with methylprednisolone （MP） on apoptosis and expression of caspase-3 mRNA and protein in early SCI, in an attempt to provide experimental clues for clinical use of EA in the treatment of early SCI. DESIGN ： A randomized control tria SETTING ： Department of Anatomy, Second Military Medical University of Chinese PLA MATERIALS： Forty-eight adult male Sprague-Dawley （SD） rats aged 12 weeks and weighing （250±20） g were used. The experimental procedures were performed in accordance with the animal care guidelines of the National Institute of Health （NIH）. Equipments and medicine： Methylprednisolone sodium succinate （Pharmacia ＆ Upjohn N.V./S.A., Puurs, Belgium）; sodium pentobarbital （Sigma Chemical Co., St. Louis, MO）; G-6805-2 Multi-Purpose Health Device （Shanghai Medical Instruments High-TECH Co., Shanghai, China）; POD kit （Roche Molecular Biochemicals, Mannheim, Germany）; ISH Detection Kit （Wuhan Boster Biological Technology Ltd., Wuhan, China）; ABC kit （Vector Laboratories, Burlingame, CA）; light microscope （x200, Olympus, Tokyo, Japan）; HPIAS-1000 color High-definition pathological image analysis system （Qianping Imaging Engineering Company of Wuhan Tongji Medical University）. METHODS： The experiment was carried out in Department of Anatomy, Second Military Medical University of Chinese PLA between October 2004 and March 2006. ① The SCI model was established by modified Allen＇s method on T10. The force applied in traumatization was 50 ***. ②Forty-eight SD rats were equally randomized into four groups： sham operation （SO） group, in which animals received laminectomy only; model control （MC） group as a negative control, in which animals did not receive any treatment; EA treatment （EA） group, in which an

关键词： TUNEL SCI

Regulation of conduction velocity for engineered cardiac conduction tissues

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Regulation of conduction velocity for engineered cardiac con...

Accelerating nerve regeneration and reducing muscle atrophy by bridging peripheral nerve defects with tissue- engineering nerve graft from bone marrow derived neuron-like cells

中国解剖学会2015年年会

作者： Li Xiaotong Yang Xiangqun Li Yuquan zhang Xi zhang chuansen Department of Anatomy Research Center of Regenerative MedicineSecond Military Medical University

＜正＞In the present study,we adopted several methods,including ET-1 treatment and the control of the parameter change in the culture,to regulate conduction velocity of the tissue engineered cardiac conduction bundle（TECCB）.After the conduction velocity regulation,we implanted TECCB into atrioventricular groove of rabbit hearts creating an AV conduction passway in an attempt to verify the ***,

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Accelerating nerve regeneration and reducing muscle atrophy ...

The preliminary observations of adipose-derived stem cells seeded in collagen sponge scaffold

中国解剖学会2013年年会

作者： Kong Zhengdong zhang Zhiying Lin Haiyan Liu Fang zhang chuansen Department of Anatomy Second Millitary Medical University

＜正＞Despite advances in surgical technology,repair of long-segment peripheral nerve defects remains *** the present study,we induced bone marrow derived neural stem-like cells into neuron-like cells and Schwann-like cells separately,then,created neuronal tissue engineering nerve graft（TENG） by seeding neuron-like cells and Schwann-like cells at a ratio of 1:1 to the acellular nerve graft TENG,and Schwann cell

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The preliminary observations of adipose-derived stem cells s...

低渗冻融联合生物酶法制备组织工程化静脉瓣脱细胞支架

中国解剖学会2012年年会

作者： Li Xiaotong zhang Xi Zhi Yunxia Yang Xiangqun zhang chuansen.Second Millary Medical University Shanghai 200433 Second Millary Medical University Shanghai 200433

＜正＞To observe the growth of adipose-derived stem cells which seeded in the collagen sponge scaffold and study the feasibility of adipose-derived stem cells（ASCs）and collagen sponge which were used as sources of seed cells and biomaterials for tissue *** inguinal adipose tissue isolated from SD rat were digested by Collagenase L After culture,purification,the ASCs were stained by CM-Dil and seeded into the collagen *** construsts

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解剖学杂志 2014年第6期37卷 731-734,799页

作者：张永珍刘兴张传森山东泰山学院体育学院理论教研室泰安271021 第二军医大学人体解剖学教研室上海200433

目的：采用低渗冻融联合生物酶法制备组织工程化静脉瓣脱细胞支架,并与先前方法制备的静脉瓣脱细胞支架比较,以期获得一种性能较好的带瓣静脉脱细胞支架材料.方法：手术获得Beagle犬带瓣静脉分为4组,对照组、脱氧胆酸钠组、Triton组和... 详细信息

目的：采用低渗冻融联合生物酶法制备组织工程化静脉瓣脱细胞支架,并与先前方法制备的静脉瓣脱细胞支架比较,以期获得一种性能较好的带瓣静脉脱细胞支架材料.方法：手术获得Beagle犬带瓣静脉分为4组,对照组、脱氧胆酸钠组、Triton组和冻融＋生物酶组.处理后对各组标本行H-E染色,透射电镜观察,DAPI荧光染色,细胞相容性测试等观察.结果：3种脱细胞方法均能彻底去除细胞,DAPI荧光检测显示各组支架材料均无DNA成分残留;H-E染色及透射电镜显示,冻融＋生物酶组胶原纤维排列整齐,未见明显的胶原纤维结构改变,其他2组可见胶原纤维断裂及结构紊乱现象;与其他2组脱细胞支架材料相比,冻融＋生物酶组的组织细胞相容性好,可见内皮祖细胞在支架材料上生长良好.结论：结合渗透压改变的反复冻融加生物酶法,既可以较彻底除去带瓣膜静脉细胞成分,又保留了较完整的细胞外基质结构,具有良好的组织和细胞相容性,是较理想的带瓣膜静脉脱细胞支架制备方法.

关键词：脱细胞支架材料组织工程化血管静脉瓣脱细胞