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Identification of porcine fast/slow myogenic exosomes and their regulatory effects on lipid accumulation in intramuscular adipocytes

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1512-1527页

作者： tiantian Zhao Tingting Tian He Yu Chaoyue Cao Ziyi zhang Zhaozhao He Zeqiang Ma Rui Cai Fengna Li Weijun Pang Laboratory of Animal Fat Deposition&Muscle Development College of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100ShaanxiChina Laboratory of Animal Nutritional Physiology and Metabolic Process Key Laboratory of Agro‑ecological Processes in Subtropical RegionInstitute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125HunanChina

Background Pork quality is affected by the type of muscle fibers, which is closely related to meat color, tenderness and juiciness. Exosomes are tiny vesicles with a diameter of approximately 30–150 nm that are secreted by cells and taken up by recipient cells to mediate communication. Exosome-mediated muscle-fat tissue crosstalk is a newly discovered mechanism that may have an important effect on intramuscular fat deposition and with that on meat quality. Various of adipose tissue-derived exosomes have been discovered and identified, but the identification and function of muscle exosomes, especially porcine fast/slow myotube exosomes, remain unclear. Here, we first isolated and identified exosomes secreted from porcine extensor digitorum longus(EDL) and soleus(SOL), which represent fast and slow muscle, respectively, and further explored their effects on lipid accumulation in longissimus dorsi *** Porcine SOL-derived exosomes(SOL-EXO) and EDL-derived exosomes(EDL-EXO) were first identified and their average particle sizes were approximately 84 nm with double-membrane disc-shapes as observed via transmission electron microscopy and scanning electron microscopy. Moreover, the intramuscular fat content of the SOL was greater than that of the EDL at 180 days of age, because SOL intramuscular adipocytes had a stronger lipid-accumulating capacity than those of the EDL. Raman spectral analysis revealed that SOL-EXO protein content was much greater than that of EDL-EXO. Proteomic sequencing identified 72 proteins that were significantly differentially expressed between SOL-EXO and EDL-EXO, 31 of which were downregulated and 41 of which were upregulated in *** Our findings suggest that muscle-fat tissue interactions occur partly via SOL-EXO promoting adipogenic activity of intramuscular adipocytes.

关键词： Adipogenesis Exosome Extensor digitorum longus Intramuscular adipocyte Muscle-fat tissue interaction Pig Soleus

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基于分子动力学的正十八烷/石墨层吸附行为分析

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东南大学学报（自然科学版） 2020年第6期50卷 1069-1075页

作者：张田田陈振乾东南大学能源与环境学院南京210096

为模拟石墨层/正十八烷复合相变材料中的烷烃分子在石墨层表面的吸附行为,构建了由石墨层和2层正十八烷分子组成的“三明治”复合相变材料微观模型,研究了体系中正十八烷分子的排布、自扩散系数等参量.最后,采用径向分布曲线对烷烃分子... 详细信息

为模拟石墨层/正十八烷复合相变材料中的烷烃分子在石墨层表面的吸附行为,构建了由石墨层和2层正十八烷分子组成的“三明治”复合相变材料微观模型,研究了体系中正十八烷分子的排布、自扩散系数等参量.最后,采用径向分布曲线对烷烃分子在不同温度下的结合强度进行分析.结果表明:石墨层对烷烃分子产生了明显的吸附作用;随着温度的升高,正十八烷分子的自扩散系数显著增加,当温度从253 K增加到283 K时,自扩散系数也从0.951 nm^2/ns逐渐增加到1.526 nm^2/ns,而在293~313 K温度范围内,自扩散系数从1.655 nm^2/ns显著增加到3.356 nm^2/ns;体系中烷烃分子自扩散系数快速增大的原因可能是发生了相变,且烷烃发生相变的温度是在303~313 K的过渡区间内.

关键词：定型相变材料分子动力学模拟均方位移自扩散系数

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Exploring social-ecological system resilience in South China Karst:Quantification,interaction and policy implication

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Geography and Sustainability 2024年第2期5卷 289-301页

作者： tiantian Chen Yuxi Wang Li Peng Chongqing Key Laboratory of Surface Process and Ecological Restoration in the Three Gorges Reservoir Area Chongqing Normal UniversityChongqing 401331China Chongqing Field Observation and Research Station of Surface Ecological Process in the Three Gorges Reservoir Area Chongqing 401331China College of Geography and Resources Sichuan Normal UniversityChengdu 610066China

China’s Grain to Green Program(GTGP),which is one of the largest payments for ecosystem services(PES)in the world,has made significant ecological improvements to the ***,current understanding of its outcomes on the social-ecological system(SES)remains ***,taking the South China Karst as an example,a SES resilience evaluation index system was constructed followed by an exploratory spatial analysis,root mean square error,and Self-Organizing Feature Map to clarify the spatiotemporal changes and relationship of SES resilience,achieve the zoning of SES resilience and provide restoration *** results showed an upward trend in social resilience from 2000 to 2020,especially its subsystem of social *** ecological resilience was stable,owing to a slightly declined ecosystem services and increased landscape ***,nearly half of the counties exhibited a distribution mismatch in SES *** was an obvious inverted U-shaped relationship of SES resilience,indicating a clear threshold effect,and the constraint relation-ship of SES resilience eased over time,demonstrating the effectiveness of the ecological restoration *** played a positive role in reducing regional SES trade-off,but this positive effect was limited,reflecting the limitations of overemphasizing the conversion from farmland to forest and *** SES resilience can be divided into four clusters,which were the key optimization zone for social system,the SES resilience safety zone,the key restoration zone for SES resilience,and the key optimization zone for ecological *** adjustments for the GTGP in these zones should be taken to achieve maximum SES benefits in the future.

关键词： SES resilience Trade-off Clustering GTGP South China Karst

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Effects of Hydroxyl Groups in Organic Ammonium Counterions on the Properties of Fluorinated Surfactants

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China Detergent & Cosmetics 2017年第4期2卷 57-65页

作者： zhang tiantian Zhou Hongyu Wu Bowan Xiao Jinxin Xing Hang Longdong University-Fluobon Surfactant Engineering Technology Center College of Chemistry and Chemical EngineeringLongdong University Beijing FLUOBON Surfactant Institute

The surface tension, foaming and viscosity for C7F15COO^-N^＋H（C2H4OH）χ（C2Hs）3-x and C8F17SO3- N^＋H（C2H4OH）χ（C2H5）3-χ （x = 0, 1, 2, 3） were measured to systematically study the effects of hydroxyl groups in organic ammonium counterions on the properties of perfluorooctanoates and perfluorooctanesulfonates. The results showed that the critical micelle concentration （cmc） and the surface tension at cmc （γcmc） were both increased with the increase of number of hydroxyl groups （χ） in the two series. The perfluorooctanesulfonate had smaller cmc but higher γ=cmc than perfluorooctanoate with the same counterion. The minimum molecular area of surface adsorption （Amin） of C8F17zSO3^-N^＋H（C2H4OH）χ（C2H5）3-χ were 0.64, 0.57, 0.63 and 0.72 nm^2 while the Amin values of C7F15COO^-N^＋H（C2H4OH）χ（C2H5）3-χ were 0.61, 0.62, 0.61 and 0.71 nm^2 when χ = 0, 1, 2 and 3, respectively. For the investigated systems of surfactants, the results of foam expansion ratio agreed with those of surface activity, while the 25% drainage time were consistent with the results of viscosity.

关键词： counter-ion organic ammonium ion fluorocarbon surfactant foam surface activity micellization

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High performance wide bandgap perovskite solar cell with low V_(OC) deficit less than 0.4 V

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Journal of Energy Chemistry 2024年第4期91卷 313-322页

作者： Haikuo Guo Fuhua Hou Xuli Ning Xiaoqi Ren Haoran Yang Rui Liu tiantian Li Chengjun Zhu Ying Zhao Wei Li Xiaodan zhang School of Physical Science and Technology Key Laboratory of Semiconductor Photovoltaic Technology and Energy Materials at Universities of Inner Mongolia Autonomous RegionInner Mongolia UniversityHohhot 010021Inner MongoliaChina Institute of Photoelectronic Thin Film Devices and Technology of Nankai University Key Laboratory of Photoelectronic Thin Film Devices and Technology of TianjinSolar Energy Research Center of Nankai UniversityTianjin 300350China State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyWuhan 430000HubeiChina

Wide bandgap perovskite solar cells(PSCs)have attracted significant attention because they can be applied to the top cells of tandem solar ***,high open-circuit voltage(V_(OC))deficit(>0.4 V)result from poor crystallization and high non-radiative recombination losses become a serious limitation in the pursuit of high ***,the relevance between different Pbl_(2)proportions and performance parameters are revealed through analysis of surface morphology,residual stress,and *** increase of Pbl_(2)proportion promotes crystal growth and reduces the work function of the perovskite film surface and promotes the energy level alignment with the carrier transport layer,which decreased the V_(OC)***,residual PbI_(2)exacerbated the stress level of perovskite film,and the resulting lattice disorder deteriorated the photostability of the ***,after the synergistic passivation of residual PbI_(2)and PEAI,the V_(OC)achieves 1.266 V and V_(OC)deficit is less than 0.4 V,the record value in wide bandgap PSCs.

关键词： Pb management Perovskite solar cell Strain Wide bandgap Stability

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Integrator,转录质控与基因组稳定的集成器

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Science Bulletin 2023年第22期68卷 2687-2688页

作者：张甜甜朱冰 National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Key Laboratory of Epigenetic Regulation and Intervention Chinese Academy of SciencesBeijing 100101China New Cornerstone Science Laboratory Institute of BiophysicsChinese Academy of SciencesBeijing 100101China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China

The Integrator complex,discovered in 2005 while searching for protein partners of deleted in split hand/split foot protein 1(DSS1),is the exclusive large multiprotein complex associated with RNA PolⅡthat is specific ... 详细信息

关键词：集成器 searching deleted

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试论“何必呢”的标记化——兼论非句法结构“何必”的词汇化

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语言科学 2013年第3期12卷 261-268页

作者：张田田上海第二工业大学人文与国际交流学院上海201209

表评注功能的语气副词"何必"由疑问词"何"与情态动词"必"构成的非句法结构发展而来。非句法结构"何必"常出现在反问语境中,由于语境吸收,表示"不必"之义,逐渐演变为一个语气副词。成词后的"何必"与其后"呢"连用形成话语标记"何必呢",语... 详细信息

表评注功能的语气副词"何必"由疑问词"何"与情态动词"必"构成的非句法结构发展而来。非句法结构"何必"常出现在反问语境中,由于语境吸收,表示"不必"之义,逐渐演变为一个语气副词。成词后的"何必"与其后"呢"连用形成话语标记"何必呢",语用强调是"何必呢"标记化的主要动因,语境吸收和语用推理是"何必呢"标记化的主要机制。话语标记"何必呢"在言语交际中具有语篇组织、隐性否定和人际互动等语用功能。

关键词：何必(呢) 词汇化标记化语用功能

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Self-assembly multifunctional DNA tetrahedron for efficient elimination of antibiotic-resistant bacteria

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Aggregate 2024年第1期5卷 204-215页

作者： tiantian Wu Yu Fu Shuang Guo Yanqiang Shi Yuxin zhang Zhijin Fan Bin Yang Baoquan Ding Yuhui Liao Molecular Diagnosis and Treatment Center for Infectious Diseases Dermatology HospitalSouthern Medical UniversityGuangzhouChina CAS Key Laboratory of Nanosystem and Hierarchical Fabrication CAS Center for Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijingChina Longgang District Central Hospital of Shenzhen ShenzhenChina

Antibiotic resistance is a major challenge in the clinical treatment of bacterial infectious ***,we constructed a multifunctional DNA nanoplatform as a versatile carrier for bacteria-specific delivery of clinical antibiotic ciprofloxacin(CIP)and classic nanoantibiotic silver nanoparticles(AgNP).In our rational design,CIP was efficiently loaded in the self-assembly double-bundle DNA tetrahedron through intercalation with DNA duplex,and single-strand DNA-modified AgNP was embedded in the cavity of the DNA tetrahedron through *** the site-specific assembly of targeting aptamer in the well-defined DNA tetrahedron,the bacteria-specific dual-antibiotic delivery system exhibited excellent combined bactericidal *** enhanced antibiotic accumulation through breaking the out membrane of bacteria,the antibiotic delivery system effectively inhibited biofilm formation and promoted the healing of infected wounds in *** DNAbased antibiotic delivery system provides a promising strategy for the treatment of antibiotic-resistant infections.

关键词： antibiotic resistance anti-infection therapy biofilm DNA nanotechnology targeted delivery

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光子晶体光纤产生紫外超连续谱的数值研究

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中国激光 2020年第3期47卷 92-97页

作者：张甜甜施伟华南京邮电大学电子与光学工程学院、微电子学院江苏南京210023

提出了一种基于高非线性氟化镁光子晶体光纤产生紫外超连续光源的方法。采用分步傅里叶法求解光纤的非线性薛定谔方程,基于光子晶体光纤数值模拟了扩展到紫外波段的超连续谱的产生;通过分析光纤结构参量与泵浦光源参数对紫外超连续谱产... 详细信息

提出了一种基于高非线性氟化镁光子晶体光纤产生紫外超连续光源的方法。采用分步傅里叶法求解光纤的非线性薛定谔方程,基于光子晶体光纤数值模拟了扩展到紫外波段的超连续谱的产生;通过分析光纤结构参量与泵浦光源参数对紫外超连续谱产生的影响,得出了光纤长度、色散参量以及泵浦脉冲峰值功率、初始脉冲宽度对超连续谱光谱宽度的影响规律。研究发现:当光子晶体光纤长度为8 cm、脉冲中心波长为450 nm、峰值功率为3.1 kW、初始脉冲宽度为40 fs时,可获得展宽至紫外的超连续谱,范围为279.6~769.0 nm。

关键词：光纤光学紫外超连续谱光子晶体光纤非线性效应色散光纤材料

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化学驱新技术及华北油田化学驱现状与展望

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精细石油化工进展 2024年第2期25卷 5-10,20页

作者：杜慧丽游靖李凤群张田田高珊珊王玥路淼森赵紫印唐雅娟华北油田公司工程技术研究院河北沧州062552 华北油田公司第三采油厂河北河间062450 华北油田公司勘探开发研究院河北沧州062552

化学驱是大幅提升油田采收率的重要技术。化学驱理论和技术持续创新,应用规模不断扩大,其中聚合物驱矿场提高采收率约14%,二元复合驱和三元复合驱矿场提高采收率均在18%以上,纳米驱油技术预期采收率可达80%以上,中相微乳液驱表面活性剂... 详细信息

化学驱是大幅提升油田采收率的重要技术。化学驱理论和技术持续创新,应用规模不断扩大,其中聚合物驱矿场提高采收率约14%,二元复合驱和三元复合驱矿场提高采收率均在18%以上,纳米驱油技术预期采收率可达80%以上,中相微乳液驱表面活性剂体系性能不断突破,矿场取得良好增油效果。针对华北油田复杂断块砂岩油藏的实际情况,采用以可动凝胶为主的深部调驱技术,进一步提高了油田采收率。为进一步提高华北油田采收率,可以在深化油藏剩余油分布规律认识的基础上,加强基础理论研究,推动化学驱技术的持续创新和有序接替,配套开发化学驱地面工程,以期实现油田开发的可持续发展。

关键词：油田采收率化学驱复合驱表面活性剂华北油田

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