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Comparison of growth and lipid accumulation properties of two oleaginous microalgae under different nutrient conditions

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Frontiers of Environmental Science & Engineering 2014年第5期8卷 703-709页

作者： Qiao zhang yu hong College of Environmental Science and Engineering Beijing Forestry University Beijing 100083 China

This study compared the growth and lipid accumulation properties of two oleaginous microalgae, namely, Scenedesmus sp. LX1 and Chlorella sp. HQ, under different nutrient conditions. Both algal species obtained the highest biomass, lipid content and lipid yield under low-nutrient conditions （mBGll medium）. The biomass, lipid content and lipid yield of Scenedesmus sp. LX1 were 0.42g·L^-1, 22.5% and 93.8mg·L^-1, respectively. These values were relatively higher than those of Chlorella sp. HQ （0.30g·L^-1, 17.1% and 51.3mg·L^-1, respectively）. These algae were then cultivated in an SE medium that contained more nutrients; as a result, the biomass and lipid yield of Scenedesmus sp. LX1 reduced more significantly than those of Chlorella sp. HQ. Opposite results were observed in lipid and triacylglycerols （TAGs） contents. The cell sizes of both algal species under low-nutrient conditions were larger than those under high-nutrient conditions. Chlorella sp. HQ cells did not aggregate, but Scenedesmus sp. LX1 cells flocculated easily, particularly under low-nutrient conditions. In summary, low-nutrient conditions favour the growth and lipid production of both algae, but Scenedesmus sp. LX1 outperforms Chlorella sp. HQ.

关键词： Scenedesmus sp. LX1 Chlorella sp. HQ,growth rate algal biomass lipid accumulation triacylgly-cerols (TAGs)

Monte Carlo Simulation of Diamond Deposition at Low Temperature

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Chinese Physics Letters 2001年第8期18卷 1138-1140页

作者： DONG Li-Fang zhang yu-hong College of Physics Science and Technology Hebei UniversityBaoding 071002

Diamond deposition at low temperature is investigated and the relationship between substrate temperature for diamond growth and the energy of the carbonaceous species is *** electron energy distribution and velocity distribution during the electron assisted chemical vapor deposition have been obtained by using Monte Carlo *** main results obtained are as follows.(1)The substrate temperature for diamond growth will be lower than 800℃ when the carbonaceous species on the substrate have mobility *** example,if the energy of the carbonaceous species is 0.75 eV,the substrate temperature will be 380℃-600℃.(2)The great number of atomic H on the substrate is of importance to the growth of diamond films.

关键词： energy. Diamond simulation.

A universal probe for simultaneous detection of six pospiviroids and natural infection of potato spindle tuber viroid(PSTVd) in tomato in China

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Journal of Integrative Agriculture 2023年第3期22卷 790-798页

作者： zhang yu-hong LI Zhi-xin DU Ya-jie LI Shi-fang zhang Zhi-xiang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China Keshan Branch of Heilongjiang Academy of Agricultural Sciences Qiqihar 161005P.R.China College of Agronomy and Biotechnology Hebei Normal University of Science&TechnologyQinhuangdao 066004P.R.China

Several viroids in the genus Pospiviroid can infect tomato(Solanum lycopersicum)and cause severe diseases,posing a serious threat to tomato *** simultaneous detection of six tomato-infecting pospiviroids-columnea latent viroid(CLVd),pepper chat fruit viroid(PCFVd),potato spindle tuber viroid(PSTVd),tomato apical stunt viroid(TASVd),tomato chlorotic dwarf viroid(TCDVd),and tomato planta macho viroid(TPMVd),we developed a universal probe based on a highly conserved 61 nt long sequence shared among *** with their specific probes,the universal probe has a similar,though slightly reduced,detection sensitivity and has the advantages of simple and cost-effective preparation and simultaneous detection of the six *** addition,the universal probe was used in dot-blot hybridization assays for a large-scale survey of viroid(s)in tomato plantings in *** PSTVd was detected in a few greenhouse-planted tomato *** analysis revealed that these tomato PSTVd isolates may have been introduced from tomato seeds imported from abroad.

关键词： tomato viroid simultaneous detection molecular hybridization pospiviroids

Synthesis and cytotoxicity studies of quinoline-3-carbonitrile derivatives

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Chinese Chemical Letters 2010年第8期21卷 939-942页

作者： Shu Lan zhang Xin Zhai Shi Jiao zhang hong Hao yu Ping Gong Key Laboratary of Original New Drugs Design and Discovery of Ministry of Education School of Pharmaceutical Engineering Shenyang Pharmaceutical UniversityShenyang 110016China

A series of quinoline-3-carbonitrile derivatives were designed and *** cytotoxicity in vitro against four cancer cell lines（A549,HT-29,MDA-MB-231 and SMMC-7721） were evaluated by standard MTT *** pharmacological results showed that most of the prepared compounds displayed excellent selective cytotoxicity toward SMMC-7721 cell *** them, compounds 7c,7e,11b,11f and 11g were more active than Gefitinb against SMMC-7721 cell line.

关键词： Quinoline-3-carbonitrile Synthesis Cytotoxicity

双偶氮苯-二苯并[b,i]噻蒽-[2,3-b]苯-5,7,12,14-四酮衍生物分子的二阶非线性光学性质

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原子与分子物理学报 2025年第2期42卷 15-23页

作者：张宇红李博陈自然李渊徐友辉张莉萍何旭东四川职业技术学院能源化工与环境学院遂宁629000

使用密度泛函理论(DFT)M06-2X方法、采用6-311+g(d,p)基组,分别对26个双偶氮-二苯并[b,i]噻蒽-[2,3-b]苯-5,7,12,14-四酮衍生物分子进行结构优化与频率计算;使用含时密度泛函理论(TD-DFT)TD-M06-2X方法计算了a1~d6分子的前线分子轨道与... 详细信息

使用密度泛函理论(DFT)M06-2X方法、采用6-311+g(d,p)基组,分别对26个双偶氮-二苯并[b,i]噻蒽-[2,3-b]苯-5,7,12,14-四酮衍生物分子进行结构优化与频率计算;使用含时密度泛函理论(TD-DFT)TD-M06-2X方法计算了a1~d6分子的前线分子轨道与电子吸收光谱,采用有效场FF方法研究了二阶非线性光学性质(NLO).研究结果表明,26个噻蒽四酮类衍生物分子的能隙在1.33—2.02 eV范围,归属于有机半导体;最低能量吸收峰波长在601.8~609.5nm范围;在增大分子的二阶非线性光学系数β_(μ)(或β_(0))值方面,含相同偶氮苯基团或含不同偶氮苯基团分别引入到二苯并[b,i]噻蒽-[2,3-b]苯-5,7,12,14-四酮分子两侧的2,10位优于2,9位,在2,10位分别端接含推、拉基团的偶氮苯优于含相同给电子基团的偶氮苯.在偶氮苯苯环对位分别端接强吸电子基(-NO_(2))与强供电子基(如-N(CH_(3))_(2)、-N(Ph)_(3)、-N-苯基咔唑等)可增强体系的二阶非线性光学性能,获得性能良好的非线性光学材料.

关键词：双偶氮二苯并[b,i]噻蒽-[2,3-b]苯-5,7,12,14-四酮密度泛函理论电子吸收光谱二阶非线性光学性质

Origin of Baotoudong syenites in North China Craton:Petrological, mineralogical and geochemical evidence

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Science China Earth Sciences 2016年第1期59卷 95-110页

作者： NIU Xiao Lu YANG Jing Sui LIU Fei zhang hong yu YANG Ming Chun State Key Laboratory of Continental Tectonics and Dynamics Institute of Geology Chinese Academy of Geological Sciences Institute of Earth Sciences China University of Geosciences Key Laboratory of Orogenic Belts and Crustal Evolution School of Earth and Space Sciences Peking University

Baotoudong syenite pluton is located to the east of Baotou City, Inner Mongolia, the westernmost part of the Trias- sic alkaline magmatic belt along the northern margin of the North China Craton （NCC）. Zircon U-Pb age, petrological, miner- alogical and geochemical data of the pluton were obtained in this paper, to constrain its origin and mantle source characteris- tics. The pluton is composed of nepheline-clinopyroxene syenite and alkali-feldspar syenite, with zircon U-Pb age of 214.7±1.1 Ma. Diopside （cores）-aegirine-augite （rims）, biotite, orthoclase and nepheline are the major minerals. The Bao- toudong syenites have high contents of rare earth elements （REE）, and are characterized by enrichment in light rare earth ele- ments （LREE） and large ion lithophile elements （LILE; e.g., Rb, Ba, Sr）, depletion in heavy rare earth elements （HREE） and high field strength elements （HFSE）. They show enriched Sr-Nd isotopic compositions with initial 87Sr/86Sr ranging from 0.7061 to 0.7067 and eNd（t） values from -9.0 to -11.2. Mineralogy, petrology and geochemical studies show that the parental magma of the syenites is SiO2-undersaturated potassic-ultrapotassic, and is characterized by high contents of CaO, Fe2O3, K2O, Na2O and fluid compositions （H2O）, and by high temperature and high oxygen fugacity. The syenites were originated from a phlogopite-rich, enriched lithospheric mantle source in garnet-stable area （〉80 km）. The occurrence of the Baotoudong sye- nites, together with many other ultrapotassic, alkaline complexes of similar ages on the northern margin of the NCC in Late Triassic implies that the lithospheric mantle beneath the northern margin of the NCC was previously metasomatized by melts/fluids from the subducted, altered paleo-Mongolian oceanic crust, and the northern margin of the craton has entered into an extensively extensional regime as a destructive continental margin in Late Triassic.

关键词： Zircon U-Pb age Mineralogy Geochemistry Baotoudong syenites Ultrapotassic alkaline rocks Triassic Mantlemetasomatism North China Craton

Diversity and Genetic Differentiation of the Whitefly Bemisia tabaci Species Complex in China Based on mtCOI and cDNA-AFLP Analysis

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Journal of Integrative Agriculture 2012年第2期11卷 206-214页

作者： GUO Xiao-jun RAO Qiong zhang Fan LUO Chen zhang hong-yu GAO Xi-wu Department of Entomology China Agricultural University Beijing 100193 P.R.China Institute of Plant and Environmental Protection Beijing Academy of Agriculture and Forestry Sciences Beijing 100097 P.R. China College of Plant Science and Technology Huazhong Agricultural University Wuhan 430070 PR. China

The whitefly Bemisia tabaci are considered as a taxonomically complex that contained some destructive *** of the most prevalent cryptic species are *** Middle East-Asia Minor 1（MEAM1）and Mediterranean（MED）.In an extensive field survey of the *** complex present throughout part of China from 2004 to 2007,we obtained 93 samples of *** from 22 *** determined that these Chinese haplotypes included 2 invasive species（MEAM1 and MED）,and 4 indigenous cryptic species（Asia II 1,Asia II 3,China 3 and Asia II 7）by sequencing mitochondrial cytochrome oxidose one gene（mtCOI）.The diversity and genetic differentiation of a subset of 19 populations of *** were studied using cDNA amplified fragment length polymorphism（AFLP）.Prior to 2007,MEAM1 was a dominant species in many provinces in *** 2007,MED was dominant in 11 *** invasive and indigenous species were simultaneously found in some *** species of *** were found in six provinces in southern *** and MEAM1 have broad ranges of host plants,and indigenous species appeared to have much narrower host *** Asia II 3 samples were found on cotton except one on *** 3 has more host plants than Asia II *** samples of China 3 were collected from sweet potato,Japanese hop,squash and cotton.A total of 677 reproducible bands amplified with 5 AFLP primer combinations were *** highest proportion of polymorphic bands was 98.7% and the lowest was 91.9%.Unweighted pair-group method analysis indicated that the clustering was independent of the different *** showed the lowest degree of similarity than the other *** data indicate that both MEAM1and MED were rapidly established in China.

关键词： Bemisia tabaci mtCOI cDNA-AFLP diversity whitefly

Aspirin increases susceptibility of Helicobacter pylori to metronidazole by augmenting endocellular concentrations of antimicrobials

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World Journal of Gastroenterology 2009年第8期15卷 919-926页

作者： Xiao-Ping zhang Wei-hong Wang yu Tian Wen Gao Jiang Li Department of Gastroenterology Peking University First Hospital Beijing 100034 China

AIM： To investigate the increasing the susceptibility pylon） to metronidazole. mechanisms of aspirin of Helicobacter pylori （H METHODS： Hpylori reference strain 26695 and two metronidazole-resistant isolates of H pylori were included in this study. Strains were incubated in Brucella broth with or without aspirin （1 mmol/L）. The rdxA gene of Hpylori was amplified by PCR and sequenced. The permeability of Hpylori to antimicrobials was determined by analyzing the endocellular radioactivity of the cells after incubated with [7-^3H]-tetracycline. The outer membrane proteins （OMPs） of Hpylori 26695 were depurated and analyzed by SDS-PAGE. The expression of 5 porins （hopA, hopB, hopC, hopD and hopE） and the putative RND efflux system （hefABC） of H pylori were analyzed using real-time quantitative PCR. RESULTS： The mutations in rdxA gene did not change in metronidazole resistant isolates treated with aspirin. The radioactivity of H pylori increased when treated with aspirin, indicating that aspirin improved the permeability of the outer membrane of H pylori. However, the expression of two OMP bands between 55 kDa and 72 kDa altered in the presence of *** expression of the mRNA of hopA, hopB, hopC, hopD, hopE and herA, hefB, hefC of H pylori did not change when treated with aspirin. CONCLUSION： Although aspirin increases the susceptibility of H pylori to metronidazole, it has no effect on the mutations of rdxA gene of Hpylori. Aspirin increases endocellular concentrations of antimicrobials probably by altering the OMP expression.

关键词： Helicobacter pylori Aspirin Metronidazole Resistance Minimum inhibitory concentrations

Unprecedented α-Octamolybdate Derivative Containing Hexad-track Cu―bpe Chains,2{[Cu(bpe)]_6[α-Mo_8O_(26)]_(3/2)}·[Cu(bpe)]_3·3H_2O

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Chemical Research in Chinese Universities 2011年第2期27卷 166-169页

作者： SHA Jing-quan LI Xin PENG Jun ZONG Xi-ming zhang yu-hong YAN hong Heilongjiang Provincial Key Laboratory of Biomaterials and Biological Medicine Formulation School of Pharmacy Jiamusi University Jiamusi 154007 P. R. China Key Laboratory of Polyoxometalate Science Ministry of Education Faculty of Chemistry Northeast Normal University Changchun 130024 P. R. China

A new inorganic-organic hybrid constructed from α-octamolybdate polyoxometalates modified by hexad- track Cu―bpe chains, 2{[Cu（bpe）]6[α-Mo8O26]3/2}·[Cu（bpe）]3·3H2O[1, bpe=bis（4-pyridyl）ethylene], has been synthesized and structurally characterized. X-Ray diffraction analysis reveals that each α-octamolybdate cluster links six transition metal complex chains and the title compound has represented the highest connective number of M―N chains modified octamolybdate POMs to date. Note that there are two crystallographically unique [α-Mo8O26]n- clusters and eight unique copper sites, which play an important role in the overall architecture of hybrid compounds. Additionally, the electrochemical properties of the title compound were studied.

关键词： Octamolybdate Hydrothermal synthesis Multi-track chain Electrochemical property