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Isobaric enzymatic assays reveal depth-dependent variation in microbial ectoenzyme activities in the deepest ocean on Earth

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Science China Earth Sciences 2025年第1期68卷 185-194页

作者： Junwei CAO Jiahua WANG Hongge zhang Junhao DENG Binbin PAN Jiawang CHEN Li zhang Jiasong FANG College of Oceanography and Ecological Science Shanghai Ocean University Ocean College Zhejiang University Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology

Microbial ectoenzymes play essential roles in the transformation and mineralization of organic matter in the ***, conventional methods for measuring microbial ectoenzyme activities(MEAs) in the deep ocean under atmospheric conditions can misrepresent the metabolic activities of indigenous microbial communities. To overcome this limitation, we measured the activities of three microbial ectoenzymes(aminopeptidase, α-glucosidase, and β-glucosidase) under both atmospheric and in situ pressure conditions in seawater collected using pressure-retaining and non-pressure-retaining samplers in the Challenger Deep, Mariana Trench. In the case of aminopeptidase and α-glucosidase, the highest isobaric MEAs were observed in the surface layer(50 m), followed by those at abyssopelagic depths(4,000–6,000 m) for aminopeptidase, hadal realm(10,903 m)for α-glucosidase, while β-glucosidase activity exhibited the highest value at 10,903 m. Furthermore, the isobaric MEAs in hadal waters were commonly found to be higher than the decompressed counterparts, highlighting the importance of pressure-retaining sampling and isobaric enzymatic assays. The half-saturation constant(Km) showed a general decreasing trend with depth,suggesting that the deep ocean microbes might have adapted to the high-pressure and oligotrophic environment by increasing their ectoenzyme's affinity to substrate. Furthermore, particle-free MEAs contribute more to the total MEAs in the deep ocean than particle-associated MEAs, suggesting the significance of cell-associated and dissolved ectoenzymes in ocean *** study provides a foundation for future investigations of MEAs in the ocean and has important implications for understanding the dynamics of microbially mediated biogeochemical cycling in marine ecosystems.

关键词： Pressure-retaining sampling Microbial ectoenzyme activity Deep-sea microbes High pressure Mariana Trench

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精细化管理在手术室骨科外来器械与植入物中的应用

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中国临床护理 2024年第5期16卷 305-307页

作者：陈红李贝张春瑾钟浩刘静华中科技大学同济医学院附属同济医院手术室武汉430030

目的探讨精细化管理在骨科外来器械与植入物管理中的应用效果。方法将2022年1月-3月进行的1275例骨科植入物手术设为对照组,将2022年4月-6月进行的骨科植入物手术1023例设为干预组,对照组采用常规的骨科手术配合管理模式,干预组采用精... 详细信息

目的探讨精细化管理在骨科外来器械与植入物管理中的应用效果。方法将2022年1月-3月进行的1275例骨科植入物手术设为对照组,将2022年4月-6月进行的骨科植入物手术1023例设为干预组,对照组采用常规的骨科手术配合管理模式,干预组采用精细化管理模式。结果干预组外来器械与植入物管理质量均明显高于对照组(均P<0.05),术中清点意外事件发生率显著低于对照组(P<0.05)。结论精细化管理能有效实施骨科外来器械与植入物的环节控制,显著提升外来器械与植入物管理质量,同时降低术中清点意外事件发生率。

关键词：多部门协同精细化管理骨科手术外来器械植入物

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DDoS Attack Autonomous Detection Model Based on Multi-Strategy Integrate Zebra Optimization Algorithm

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Computers, Materials & Continua 2025年第1期82卷 645-674页

作者： Chunhui Li Xiaoying Wang Qingjie zhang Jiaye Liang Aijing zhang School of Information Engineering Institute of Disaster PreventionLangfang065201China Langfang Key Laboratory of Network Emergency Protection and Network Security Langfang065201China

Previous studies have shown that deep learning is very effective in detecting known ***,when facing unknown attacks,models such as Deep Neural Networks(DNN)combined with Long Short-Term Memory(LSTM),Convolutional Neural Networks(CNN)combined with LSTM,and so on are built by simple stacking,which has the problems of feature loss,low efficiency,and low ***,this paper proposes an autonomous detectionmodel for Distributed Denial of Service attacks,Multi-Scale Convolutional Neural Network-Bidirectional Gated Recurrent Units-Single Headed Attention(MSCNN-BiGRU-SHA),which is based on a Multistrategy Integrated Zebra Optimization Algorithm(MI-ZOA).The model undergoes training and testing with the CICDDoS2019 dataset,and its performance is evaluated on a new GINKS2023 *** hyperparameters for Conv_filter and GRU_unit are optimized using the Multi-strategy Integrated Zebra Optimization Algorithm(MIZOA).The experimental results show that the test accuracy of the MSCNN-BiGRU-SHA model based on the MIZOA proposed in this paper is as high as 0.9971 in the CICDDoS 2019 *** evaluation accuracy of the new dataset GINKS2023 created in this paper is *** to the MSCNN-BiGRU-SHA model based on the Zebra Optimization Algorithm(ZOA),the detection accuracy on the GINKS2023 dataset has improved by 5.81%,precisionhas increasedby 1.35%,the recallhas improvedby 9%,and theF1scorehas increasedby 5.55%.Compared to the MSCNN-BiGRU-SHA models developed using Grid Search,Random Search,and Bayesian Optimization,the MSCNN-BiGRU-SHA model optimized with the MI-ZOA exhibits better performance in terms of accuracy,precision,recall,and F1 score.

关键词： Distributed denial of service attack intrusion detection deep learning zebra optimization algorithm multi-strategy integrated zebra optimization algorithm

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Integrated Equipment with Functions of Current Flow Control and Fault Isolation for Multiterminal DC Grids

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Energy Engineering 2025年第1期122卷 85-99页

作者： Shuo zhang Guibin Zou School of Electrical Engineering Shandong UniversityJinan250061China Suzhou Research Institute Shandong UniversitySuzhou215123China

The multi-terminal direct current(DC)grid has extinctive superiorities over the traditional alternating current system in integrating large-scale renewable *** the DC circuit breaker(DCCB)and the current flow controller(CFC)are demanded to ensure the multiterminal DC grid to operates reliably and ***,since the CFC and the DCCB are all based on fully controlled semiconductor switches(e.g.,insulated gate bipolar transistor,integrated gate commutated thyristor,etc.),their separation configuration in the multiterminal DC grid will lead to unaffordable implementation costs and conduction power *** solve these problems,integrated equipment with both current flow control and fault isolation abilities is proposed,which shares the expensive and duplicated components of CFCs and DCCBs among adjacent *** addition,the complicated coordination control of CFCs and DCCBs can be avoided by adopting the integrated equipment in themultiterminal DC *** order to examine the current flow control and fault isolation abilities of the integrated equipment,the simulation model of a specific meshed four-terminal DC grid is constructed in the PSCAD/EMTDC ***,the comparison between the integrated equipment and the separate solution is presented a specific result or conclusion needs to be added to the abstract.

关键词： Integrated equipment multiterminal direct current grid current flow control fault isolation

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Aperiodically semi-intermittent-based fixed-time stabilization and synchronization of delayed discontinuous inertial neural networks

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Science China(Information Sciences) 2025年第1期68卷 238-254页

作者： Guodong zhang Jinde CAO School of Mathematics and Statistics South-Central Minzu University School of Mathematics Southeast University

This article concentrates on the fixed-time stabilization(FS) and fixed-time synchronization(FTS) of discontinuous inertial neural networks(DINNs) with distributed delays. Using mathematical induction to a new differential equality, a novel fixed-time stability lemma is constructed. Then, by designing an aperiodically semi-intermittent switching control and combining it with the theory of nonsmooth analysis, some novel criteria on the FS and FTS of DINNs are obtained. Unlike the methods used in most existing studies, the FS and FTS results are structured using the newly proposed fixed-time stability lemma and the nonreduced-order approach, resulting in broader practical applications and strengthening the scientific quality of the derived results. Lastly, numerical simulations and applications proffer the effectiveness of the established FS and FTS criteria.

关键词： inertial neural networks fixed-time synchronization semi-intermittent switching control nonreduced-order approach fixed-time stabilization

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Targeting Epac2 and GluA3-containing AMPARs:a novel therapeutic strategy for Alzheimer's disease

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Neural Regeneration Research 2025年第11期20卷 3223-3224页

作者： Tong zhang Martina Schmidt Department of Molecular Pharmacology Groningen Research Institute of PharmacyUniversity of GroningenGroningenThe Netherlands School of Pharmacy Tianjin Medical UniversityTianjinChina

Alzheimer's disease(AD)is a neurodegenerative disease that manifests progressive decline in memory and *** the early stage of AD,memory retrieval is impaired preceding memory acquisition and consolidation(Roy et al.,2016).Prior to the onset of symptoms,pathological amyloid-β(Aβ)plagues and tau protein tangles accumulate in extracellular and intracellular spaces,respectively,leading to *** these hallmark pathologies,Aβ is proposed to be the primary etiology by triggering a cascade of pathogenic events,including neuroinflammation,oxidative stress,tau hyperphosphorylation,synaptic/neuronal dysfunction,and neuronal death(zhang et al.,2023b).

关键词： Alzheimer impaired Aβ

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Subretinal fibrosis secondary to neovascular age-related macular degeneration:mechanisms and potential therapeutic targets

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Neural Regeneration Research 2025年第2期20卷 378-393页

作者： Jingxiang zhang Xia Sheng Quanju Ding Yujun Wang Jiwei Zhao Jingfa zhang Department of Ophthalmology People’s Hospital of Huangdao DistrictQingdaoShandong ProvinceChina Department of Urology People’s Hospital of Huangdao DistrictQingdaoShandong ProvinceChina Department of Ophthalmology Shanghai General Hospital(Shanghai First People’s Hospital)Shanghai Jiao Tong University School of MedicineShanghaiChina National Clinical Research Center for Eye Diseases Shanghai Key Laboratory of Ocular Fundus DiseasesShanghai Engineering Center for Visual Science and PhotomedicineShanghai Engineering Center for Precise Diagnosis and Treatment of Eye DiseasesShanghaiChina

Subretinal fibrosis is the end-stage sequelae of neovascular age-related macular *** causes local damage to photoreceptors,retinal pigment epithelium,and choroidal vessels,which leads to permanent central vision loss of patients with neovascular age-related macular *** pathogenesis of subretinal fibrosis is complex,and the underlying mechanisms are largely ***,there are no effective treatment options.A thorough understanding of the pathogenesis of subretinal fibrosis and its related mechanisms is important to elucidate its complications and explore potential *** current article reviews several aspects of subretinal fibrosis,including the current understanding on the relationship between neovascular age-related macular degeneration and subretinal fibrosis;multimodal imaging techniques for subretinal fibrosis;animal models for studying subretinal fibrosis;cellular and non-cellular constituents of subretinal fibrosis;pathophysiological mechanisms involved in subretinal fibrosis,such as aging,infiltration of macrophages,different sources of mesenchymal transition to myofibroblast,and activation of complement system and immune cells;and several key molecules and signaling pathways participating in the pathogenesis of subretinal fibrosis,such as vascular endothelial growth factor,connective tissue growth factor,fibroblast growth factor 2,platelet-derived growth factor and platelet-derived growth factor receptor-β,transforming growth factor-βsignaling pathway,Wnt signaling pathway,and the axis of heat shock protein 70-Toll-like receptors 2/*** review will improve the understanding of the pathogenesis of subretinal fibrosis,allow the discovery of molecular targets,and explore potential treatments for the management of subretinal fibrosis.

关键词： choroidal neovascularization epithelial-mesenchymal transition mesenchymal transition myofibroblast neovascular age-related macular degeneration submacular fibrosis subretinal fibrosis therapeutic targets transforming growth factor-β vascular endothelial growth factor

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Glucocorticoid receptor signaling in the brain and its involvement in cognitive function

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Neural Regeneration Research 2025年第9期20卷 2520-2537页

作者： Chonglin Su Taiqi Huang Meiyu zhang Yanyu zhang Yan Zeng Xingxing Chen Brain Science and Advanced Technology Institute Hubei Province Key Laboratory of Occupational Hazard Identification and ControlSchool of MedicineWuhan University of Science and TechnologyWuhanHubei ProvinceChina

The hypothalamic-pituitary-adrenal axis regulates the secretion of glucoco rticoids in response to environmental *** the brain,a nuclear receptor transcription fa ctor,the glucocorticoid recepto r,is an important component of the hypothalamicpituitary-a d renal axis's negative feedback loop and plays a key role in regulating cognitive equilibrium and *** glucoco rticoid receptor influences cognitive processes,including glutamate neurotransmission,calcium signaling,and the activation of brain-derived neurotrophic factor-mediated pathways,through a combination of genomic and non-genomic *** interactions within the central nervous system can alter the expression and activity of the glucocorticoid receptor,there by affecting the hypothalamic-pituitary-a d renal axis and stress-related cognitive *** appropriate level of glucocorticoid receptor expression can improve cognitive function,while excessive glucocorticoid receptors or long-term exposure to glucoco rticoids may lead to cognitive *** with cognitive impairment-associated diseases,such as Alzheimer's disease,aging,depression,Parkinson's disease,Huntington's disease,stroke,and addiction,often present with dysregulation of the hypothalamic-pituitary-adrenal axis and glucocorticoid receptor *** review provides a comprehensive overview of the functions of the glucoco rticoid receptor in the hypothalamic-pituitary-a d renal axis and cognitive *** emphasizes that appropriate glucocorticoid receptor signaling fa cilitates learning and memory,while its dysregulation can lead to cognitive *** provides clues about how glucocorticoid receptor signaling can be targeted to ove rcome cognitive disability-related disorders.

关键词： brain-derived neurotrophic factor calcium signaling glucocorticoid receptor glucocorticoid glutamate transmission hypothalamic-pituitary-adrenal axis long-term potentiation neurocognitive disorders neuroplasticity stress

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Multiphysics simulation and optimization of the in-situ beneficiation process of ilmenite minerals from lunar regolith

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Science China Earth Sciences 2025年第1期68卷 195-207页

作者： Peng zhang Xin LIU Guanghui LIU Wei DAI Hanzhe YANG Haibo ZHENG Zhi WANG Ran NIU Yifan BAI Yang zhang Chengbao LIU Ge YANG Junwei YANG Guang zhang Technology and Engineering Center for Space Utilization Chinese Academy of Sciences Beijing Hegong Simulation Co. Ltd

The exploration and utilization of lunar resources have become the major objectives of future lunar exploration missions. The classified collection and processing of lunar minerals is the basic prerequisite for the in-situ exploration and utilization of lunar resources. In this study, we propose a scheme for in-situ separation of magnetic minerals from lunar soils using the dry magnetic separation method. The scheme can realize the purpose of separating magnetic minerals from lunar regolith by altering the motion trajectory of lunar regolith particles with different magnetic properties. Detailed modeling of the dry magnetic separation device is conducted, and the three-dimensional distribution of magnetic parameters is calculated and analyzed. Based on the characterization and analysis of the Chang E-5's samples, the morphologies and physical parameters of lunar regolith particles are modeled and simulated. A discrete element simulation of the motion process of lunar regolith particles under multi-physical field conditions is performed, and the influences of key parameters on the beneficiation performance of lunar magnetic minerals are investigated. Our results indicate that optimal separation and beneficiation of lunar regolith particles can be achieved under specific conditions of magnetic field strength and drum speed. Additionally, we analyze the effects of lunar weak gravity conditions and the charged nature of lunar particles on the beneficiation process, showing that these factors can obviously enhance the beneficiation process of lunar ilmenites. This study demonstrates the feasibility of using the dry magnetic separation method for the beneficiation of magnetic minerals from lunar regolith, providing a theoretical basis for the scientific tasks of collecting, processing, and utilizing lunar mineral resources.

关键词： Lunar regolith In-situ resource utilization Magnetic separation Classified sampling

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Molecular dynamics simulations of helium transport through inorganic mineral nanopores

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Science China Earth Sciences 2025年第1期68卷 237-252页

作者： Dandan SONG Ping GUAN Chi zhang Jiahao REN School of Earth and Space Sciences Peking University

Helium transport through nanoscale inorganic mineral pores and pore throats is essential for its overall migration. To elucidate helium's transport dynamics within nanopores, we employed equilibrium and non-equilibrium molecular dynamics simulations to investigate helium's static self-diffusion and pressure-driven flow in quartz slit-shaped nanopores. We also introduced water and various gases, including hydrogen, methane, ethane, nitrogen, and carbon dioxide, into the nanopores to assess their influence on helium transport. Our findings indicate minimal helium adsorption on quartz pore surfaces. Under conditions where the pore size is less than 5 nm and the pressure under 10 MPa, environmental factors markedly influence helium diffusion. Large pore sizes, high temperatures, and low gas pressures enhance helium desorption and facilitate faster diffusion. We observed a positive correlation between helium flow velocity and factors such as pore size, pressure gradient, and surface smoothness of the pores. Notably, the presence of pore water and carrier gases in quartz nanopores, which diffuse more slowly than helium, tends to reduce helium surface adsorption and slow its diffusion. Among the carrier gases studied, nitrogen showed similar adsorption capacity, diffusivity, and stability to helium, while carbon dioxide displayed the highest adsorption capacity and the slowest diffusion rate, markedly differing from helium. Based on the simulation results, we concluded that water and carrier gases primarily function as transport mediums in helium migration, moving together with helium. Nitrogen, which shares similar properties with helium, effectively assists in this co-migration process. Conversely, carbon dioxide, due to its high adsorption capacity and slow diffusion, tends to be lost during co-migration. As a result, gas reservoirs with high nitrogen levels and low carbon dioxide levels are more likely to have higher helium concentrations. Additionally, the smaller por

关键词： Helium transport Slit nanopores Molecular dynamics simulations Diffusion Flow carriers

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