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Validating the use of ROS-scavenging bacteria as probiotics to increase coral resilience to thermal stress

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Journal of Oceanology and Limnology 2024年第4期42卷 1242-1260页

作者： Xiaoyu TANG Qingsong YANG Ying zhang Hanzhang WANG Juan LING Haiyan SUN Junde DONG yanying zhang CAS Key Laboratory of Tropical Marine Bio-resources and Ecology South China Sea Institute of Oceanology(SCSIO)Guangzhou 510301China Ocean School Yantai UniversityYantai 264005China Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) Guangzhou 511458China Sanya National Marine Ecosystem Research Station and Key Laboratory of Tropical Marine Biotechnology of Hainan Province Sanya Institute of OceanologySCSIOSanya 572000China Institute of Deep-sea Science and Engineering Chinese Academy of SciencesSanya 572000China University of Chinese Academy of Sciences Beijing 100049China

Thermal stress causes the overproduction and toxic accumulation of reactive oxygen species(ROS),which seems to be correlated with coral bleaching and,ultimately,*** reduction of ROS concentration within the coral holobiont could minimize the effects of thermal stress and support efforts to reduce coral decline *** the current study,we explored the physiological responses of Pocillopora damicornis to ROS-scavenging bacteria inoculation as well as the microbiome restructuring that correlates with ***’s resilience to thermal stress after probiotic *** of corals with ROS-scavenging bacteria enhanced coral health and reduced ROS ***,the enhanced coral thermal resistance promoted by ROS-scavenging bacteria was also correlated with an overall coral microbiome *** addition,the complex network relationships between bacteria and Symbiodiniaceae in corals after ROS-scavenging bacteria inoculation contributed to corals’resilience to high ***,coral heat tolerance bacterial biomarkers,such as Myxococcota,were enriched in corals with added ROS-scavenging ***,our findings validate the selected ROS-scavenging bacteria as coral probiotics that could help corals resist thermal stress on a short ***,our data contribute to our understanding of the potential interactions between different members of the coral holobiont and the use of probiotics as tools to aid coral restoration efforts.

关键词： coral beneficial microorganisms for corals(BMCs) Symbiodiniaceae thermal stress coral bleaching

Microstructure and Hot Tearing Sensitivity Simulation and Parameters Optimization for the Centrifugal Casting of Al-Cu Alloy

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Computers, Materials & Continua 2024年第8期80卷 2873-2895页

作者： Xueli He Shengkun Lv Ruifeng Dou yanying zhang Junsheng Wang Xunliang Liu Zhi Wen School of Energy and Environmental Engineering(SEEE) University of Science and Technology Beijing(USTB)Beijing100083China Advanced Research Institute of Multidisciplinary Science(ARIM) Beijing Institute of Technology(BIT)Beijing100081China

Four typical theories on the formation of thermal tears:strength,liquid film,intergranular bridging,and solidifica-tion shrinkage compensation *** these theories,a number of criteria have been derived for predicting the formation of thermal cracks,such as the stress-based Niyama,Clyne,and RDG(Rapaz-Dreiser-Grimaud)*** this paper,a mathematical model of horizontal centrifugal casting was established,and numerical simulation analysis was conducted for the centrifugal casting process of cylindrical Al-Cu alloy castings to investigate the effect of the centrifugal casting process conditions on the microstructure and hot tearing sensitivity of alloy castings by using the modified RDG hot tearing *** show that increasing the centrifugal rotation and pouring speeds can refine the microstructure of the alloy but increasing the pouring and mold preheating temperatures can lead to an increase in grain *** grain size gradually transitions from fine grain on the outer layer to coarse grain on the inner ***,combined with the modified RDG hot tearing criterion,the overall distribution of the castings’hot tearing sensitivity was *** analysis results indicate that the porosity in the middle region of the casting was large,and hot tearing defects were prone to *** hot tearing tendency on the inner side of the casting was greater than that on the outer *** effects of centrifugal rotation speed,pouring temperature,and preheating temperature on the thermal sensitivity of Al-Cu alloy castings are summarized in this *** study revealed that the tendency of alloy hot cracking decreases with the increase of the centrifugal speed,and the maximum porosity of castings decreases first and then increases with the pouring *** the preheating temperature increases,the overall maximum porosity of castings shows a decreasing trend.

关键词： Centrifugal casting Al-Cu alloy microstructure hot tearing simulation

银狐源沙门菌的分离鉴定、致病性及耐药性检测

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中国动物传染病学报 2024年第4期32卷 111-117页

作者：张召兴韩平李涵张艳英史秋梅河北旅游职业学院畜牧兽医系承德067000 河北科技师范学院河北省预防兽医学重点实验室秦皇岛066604

为了解秦皇岛地区不同养殖场中银狐源沙门菌致病性、耐药情况及耐药基因的携带情况,本研究2019—2021年采集秦皇岛地区不同养殖场中患腹泻病银狐的肛拭子、粪便等病料组织194份,采用RT-PCR、PCR方法对采集的样品检测其犬瘟热病毒、轮状... 详细信息

为了解秦皇岛地区不同养殖场中银狐源沙门菌致病性、耐药情况及耐药基因的携带情况,本研究2019—2021年采集秦皇岛地区不同养殖场中患腹泻病银狐的肛拭子、粪便等病料组织194份,采用RT-PCR、PCR方法对采集的样品检测其犬瘟热病毒、轮状病毒及犬细小病毒;采用细菌常规分离鉴定、PCR方法进行沙门菌鉴定,采用人工感染小白鼠试验、K-B药敏纸片法和PCR法分别检测沙门菌的致病性、耐药性及耐药基因型。结果显示,犬瘟热病毒、冠状病毒及犬细小病毒检测为阴性;从194份病料组织中分离得到101株沙门菌,其中78株沙门菌分离菌株对小白鼠具有致病,死亡率为40%~100%;78株致病性沙门菌对氨苄西林、阿莫西林、土霉素等9种药物耐药率在50%以上,对其他药物耐药率为5.1%~32.1%,以耐12、11、10种药物为主;耐药基因Sul1、Sul2、Sul3、aac(3)-Ⅰv、aac(6')-Ⅰb、TetA、TetM、TetR检出率在48.7%以上,其他耐药基因检出率为7.7%~21.8%,除了多粘菌素类药物,耐药表型与耐药基因之间存在相关性。本研究对该地区银狐源沙门菌病防控提供参考依据。

关键词：银狐腹泻沙门菌致病性耐药性耐药基因

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Recent trends of machine learning applied to multi-source data of medicinal plants

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Journal of Pharmaceutical Analysis 2023年第12期13卷 1388-1407页

作者： yanying zhang Yuanzhong Wang Medicinal Plants Research Institute Yunnan Academy of Agricultural SciencesKunming650200China College of Traditional Chinese Medicine Yunnan University of Chinese MedicineKunming650500China

In traditional medicine and ethnomedicine,medicinal plants have long been recognized as the basis for materials in therapeutic applications *** particular,the remarkable curative effect of traditional Chinese medicine during corona virus disease 2019(COVID-19)pandemic has attracted extensive attention *** plants have,therefore,become increasingly popular among the ***,with increasing demand for and profit with medicinal plants,commercial fraudulent events such as adulteration or counterfeits sometimes occur,which poses a serious threat to the clinical outcomes and interests of *** rapid advances in artificial intelligence,machine learning can be used to mine information on various medicinal plants to establish an ideal resource *** herein present a review that mainly introduces common machine learning algorithms and discusses their application in multi-source data analysis of medicinal *** combination of machine learning algorithms and multi-source data analysis facilitates a comprehensive analysis and aids in the effective evaluation of the quality of medicinal *** findings of this review provide new possibilities for promoting the development and utilization of medicinal plants.

关键词： Machine learning Medicinal plant Multi-source data Data fusion Application

Dynamic random channel reservation protocol

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Journal of Systems Engineering and Electronics 2005年第4期16卷 721-727页

作者： Ren yanying zhang Wenjun Yu Songyu Inst. of Image Communication and Information Processing Shanghai Jiaotong Univ. Shanghai 200030 P. R. China

Demand assignment MAC protocols have been used widely in wireless networks. It can effectively utilize wireless bandwidth. Some strategies can he used by demand assignment MAC protocols to further improve their efficiency. The concept of transmit probability is introduced. This concept allows a request slot to be assigned to many different traffic classes at the same time. Based on it, the dynamic random channel reservation （DRCR） protocol is proposed. The DRCR protocol operates dynamically by observing the traffic conditions. It uses information about the recent traffic conditions to assign transmit probability with which an mobile station can select request slots with lower traffic. The performance of DRCR is evaluated and compared with RSCA. The results show that DRCR is more stable than RSCA, it offers shorter delays of requests than RSCA and can relieve heavily stressed traffic classes faster than RSCA.

关键词： dynamic random channel reservation protocol transmit probability demand assignment MAC protocol.

冀北地区养殖场奶/肉犊牛腹泻中志贺菌的分离鉴定及特征分析

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中国兽医学报 2024年第3期44卷 477-483页

作者：张召兴赵允清王珍珊张妍衡江鸿李佩国张艳英河北旅游职业学院畜牧兽医系河北承德067000 河北科技师范学院河北省预防兽医学重点实验室河北秦皇岛066604

为鉴定引起冀北地区养殖场中奶/肉犊牛腹泻的病原菌并分析其生物学特征,本研究于2022-2023年采集冀北地区养殖场患腹泻病奶/肉犊牛腹泻的肛拭子、粪便及病料组织273份,采用细菌分离培养、生化鉴定及PCR方法对志贺菌(Shigella)进行分离鉴... 详细信息

为鉴定引起冀北地区养殖场中奶/肉犊牛腹泻的病原菌并分析其生物学特征,本研究于2022-2023年采集冀北地区养殖场患腹泻病奶/肉犊牛腹泻的肛拭子、粪便及病料组织273份,采用细菌分离培养、生化鉴定及PCR方法对志贺菌(Shigella)进行分离鉴定,采用人工感染小鼠试验、玻板凝集试验、PCR法和K-B药敏纸片法分别检测志贺菌的致病性、血清型、毒力基因,用人工感染小鼠试验和改良寇氏法测定其优势血清型代表株的半数致死量(LD50);采用RT-PCR对牛病毒性腹泻病毒(bovine viral diarrhea virus,BVDV)、牛轮状病毒(bovine rotavirus,BRV)、牛冠状病毒(bovine coronavirus,BCoV)、牛诺瓦病毒(bovine norovirus,BNoV)和牛纽布病毒(bovine nebovirus,BNeV)5种病原检测。结果表明,5种病毒性病原检测为阴性;从采集的病料组织中分离得到94株志贺菌,其中52株志贺菌引起小白鼠发病与致死,为致病性志贺菌,对小鼠致死率在40%以上;52株致病性志贺菌中以福氏志贺菌F2a型(21.2%)、F1b型(25.0%),宋内志贺菌Ⅰ相(19.2%)和痢疾志贺菌(13.5%)为优势流行血清型;52株致病性志贺菌毒力基因ipaH、ipa BCD、ial、Set1A、Set1B、sen、Sat、sigA的检出率在51.9%~100.0%之间;52株致病性志贺菌对磺胺间甲氧嘧啶、土霉素、多西环素等12种药物的耐药率在51.9%以上,其他药物的耐药率在5.8%~34.6%之间,分离的菌株呈现MDR且至少耐4类药物,4株优势血清型代表株HS-1(福氏志贺菌F2a型)、HS-2(福氏志贺菌F1b型)、HS-3(宋内志贺菌Ⅰ相)、HS-4(痢疾志贺菌)的LD50分别为2.51×10^(6)、5.01×10^(6)、6.68×10^(6)和2.11×10^(7)CFU/mL。本研究为冀北地区养殖场中奶/肉犊牛源志贺菌病的流行病调查与防控提供参考依据。

关键词：犊牛志贺菌血清型毒力基因耐药性 LD50

Sub-10 fs laser pulses with repetition rate of 1.1 GHz by a Ti:sapphire oscillator

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Chinese Science Bulletin 2009年第20期54卷 3649-3652页

作者： zhang Qing ZHAO yanying WEI ZhiYi Institute of Physics Chinese Academy of Sciences Beijing 100190 China

We demonstrate a compact Ti:sapphire oscillator with ring cavity *** optimizing the intra-cavity dispersion with chirped mirrors,pulses with repetition rate of 1.1 GHz are coupled out by the uncoated wedges in the *** 7W CW pump laser centered at 532 nm,the average power of the output pulses is about 30 mW,the duration is less than 10fs and the spectrum spans from 670 nm to 920 nm.

关键词：激光脉冲重复率振荡器蓝宝石 GHz 财政 32纳米钛

Diversity and geographical distribution of haptophyte Phaeocystis in the Chinese seas based on metabarcoding analysis

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Journal of Oceanology and Limnology 2023年第6期41卷 2197-2207页

作者： Qingshang SONG Yue XUE yanying zhang Jiehui YIN Pingping SHEN Ocean School Yantai UniversityYantai 264005China

Phaeocystis is an important bloom-forming species and over 100 blooms have occurred since 1997 along the Chinese coasts,while only the species *** was *** this project,a total of 246 phytoplankton samples collected from the Bohai Sea,the Yellow Sea,and the East China Sea in April 2021 were analyzed to evaluate the species diversity and geographical distribution of the genus Phaeocystis based on metabarcoding 18S r DNA *** described Phaeocystis species were recognized,including ***,***,***,and ***,and each has distinguished geographical distribution *** was the most widespread and abundant species,and *** was found in the Bohai Sea and the northern Yellow Sea with substantially elevated *** was also a widespread species,but its abundance was relatively low,while *** gathered only in the southeastern East China *** temperature,phosphate as well as ammonium contents were found to be associated with the abundance of ***,***,and *** ***,two uncharacterized Phaeocystis species were detected in the Chinese seas,indicating the diversity of the genus remains underestimated worldwide.

关键词： Haptophyta Phaeocystis species diversity distribution Chinese seas amplicon sequence variant

基于Nrf2/HO-1/GPX4信号通路探讨真武汤改善脾肾阳虚型糖尿病肾病小鼠的作用机制

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中国实验方剂学杂志 2024年第15期30卷 29-37页

作者：赵哲陈光顺白敏靳玉秋田萌媛宋冰刘臻华张延英甘肃中医药大学兰州730000 甘肃省实验动物行业技术中心兰州730000

目的:基于核因子E2相关因子2(Nrf2)/血红素氧合酶-1(HO-1)/谷胱甘肽过氧化物酶4(GPX4)信号通路探讨真武汤对脾肾阳虚型糖尿病肾病(DKD)小鼠肾脏氧化损伤的干预作用及机制。方法:将25只7周龄SPF级雄性db/m小鼠及95只7周龄SPF级雄性db/db... 详细信息

目的:基于核因子E2相关因子2(Nrf2)/血红素氧合酶-1(HO-1)/谷胱甘肽过氧化物酶4(GPX4)信号通路探讨真武汤对脾肾阳虚型糖尿病肾病(DKD)小鼠肾脏氧化损伤的干预作用及机制。方法:将25只7周龄SPF级雄性db/m小鼠及95只7周龄SPF级雄性db/db小鼠适应性喂养1周。db/m小鼠作为空白组,其余小鼠进行运用灌服生大黄溶液和氢化可的松制备脾肾阳虚型DKD模型,成模后随机分为模型组、厄贝沙坦组(25 mg·kg^(-1))、真武汤高、中、低剂量(33.8、16.9、8.45 g·kg^(-1))组,每组15只,连续灌胃8周。观察小鼠生存状态和计算中医证候评分并测定脾肾阳虚相关、空腹血糖(FBG)及肾功能相关指标;苏木素-伊红(HE)染色观察各组肾脏组织病理学改变;生化试剂盒法测定肾组织中氧化应激相关指标;实时荧光定量聚合酶链式反应(Real-time PCR)和蛋白免疫印迹法(Western blot)检测各组小鼠肾组织中Nrf2、HO-1、谷氨酸半胱氨酸连接酶催化亚基(GCLC)、GPX4 m RNA和蛋白表达水平。结果:与空白组比较,模型组小鼠中医证候积分明显升高(P<0.05),雌二醇(E2)含量明显升高(P<0.05),睾酮(T)、三碘甲状腺原氨酸(T3)、四碘甲状腺原氨酸(T4)含量明显降低(P<0.05);FBG明显升高(P<0.05),肾功能明显下降(P<0.05);肾脏病理显示肾小球肥大,肾小球系膜和基底增厚明显,肾组织中过氧化氢酶(CAT)、总抗氧化能力(T-AOC)、还原型谷胱甘肽(GSH)水平均明显降低(P<0.05),丙二醛(MDA)的含量明显升高(P<0.05),肾组织中Nrf2、HO-1、GCLC、GPX4 m RNA及蛋白表达水平明显下降(P<0.05);与模型组比较,真武汤高、中剂量组中医证候积分明显降低、E2含量明显降低(P<0.05),T、T3、T4含量明显升高(P<0.05);肾功能明显改善(P<0.05),肾脏病理明显改善,真武汤高、中剂量组小鼠肾组织中CAT、T-AOC、GSH水平均明显升高(P<0.05),MDA的含量明显下降(P<0.05),各给药组小鼠肾组织中Nrf2、HO-1、GCLC、GPX4 m RNA及蛋白表达水平明显升高(P<0.05)。结论:真武汤改善脾肾阳虚型db/db小鼠一般状态、肾功能,降低氧化损伤和减轻肾脏病理改变,其作用机制可能与调节Nrf2/HO-1/GPX4通路有关。

关键词：真武汤脾肾阳虚糖尿病肾病核因子E2相关因子2(Nrf2)/血红素氧合酶-1(HO-1)/谷胱甘肽过氧化物酶4(GPX4)信号通路氧化损伤