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Frontiers of Materials Science 2020年第4期14卷 488-496页

作者： Qingyang GU jinyan LI Liangshuo JI Ruijun JU Haibo JIN rongyue zhang Department of Chemical Engineering Beiing Institute of Petrochemical TechnologyBejing 102617China Beiing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology Beiing 102617China

We demonstrate the fabrication of a novel magnetic nanohybrid involving the drug molecule 5 aminolevulinic acid(5-ALA)intercalated Gd-Eu layered rare-earth hydroxide(LRH)coated on magnesium ferrite particles(MgFe2O4).The structure,thermostability,morphology,luminescence properties,cytotoxic effect and magnetism are *** 5-ALA intercalated composite may correspond to a monolayered vertical arrangement,and the thermal stability of organics is enhanced after *** LRH precursor shows red emission of Eu^3+and the maximum emission peak of the composite is at 451 nm,corresponding to the blue *** detection of drug molecules can be realized through the change of *** magnetic nanohybrid shows strong magnetic sensitivity,which provides an easy and efficient way to separate 5-ALA-MgFe2O4@LGd0.95H:Eu0.05 particles from a sol or a suspension system and to carry drugs to targeted locations under an external magnetic *** cytotoxic effect of MgFe2O4@LRH is observed with a sulforhodamine B(SRB)colorimetric assay,which has low cytotoxic effects on selected *** fabrication of novel bifunctional drug carriers based on LRH with magnetic and fluorescent properties has potential applications in drug detection and drug delivery.

关键词： layered rare-earth hydroxide magnesium ferrite nanohybrid magnetism luminescence

Green synthesis of gold nanoclusters using papayajuice for detection of L-lysine

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Chinese Chemical Letters 2019年第3期30卷 660-663页

作者： Tian Yu Chengnan Xu Juan Qiao rongyue zhang Li Qi Beijing National Laboratory of Molecular Sciences Key Laboratory of Analytical Chemistry for Living Bio-systems Institute of Chemistry Chinese Academy of Sciences Beijing Institute of Petro-chemical Technology University of Chinese Academy of Sciences

Gold nanoclusters were rapid synthesized within 3 min at 120 ℃ by using papaya juice as a capping and reducing agent(P-AuNCs). The properties of the fluorescent probe were characterized by fluorescent spectroscopy, UV-vis spectroscopy, dynamic light scattering and transmission electron *** on the surface electron density increase-induced fluorescence enhancing principle, a high selective method for detection of L-lysine was developed with the as-prepared P-AuNCs coupling the fluorescence emission at 440 nm. The fluorescent probe showed high stability and good biocompatibility. Its fluorescence intensity was found to be linearly dependent on the L-lysine concentration in the range of 10.0μmol/L to 1000.0 μmol/L(R^2=0.969) with a limit of detection of 6.0μmol/L. Furthermore, the PAuNCs based approach was applied for monitoring the urine L-lysine contents, demonstrating great potential of fluorescent probes in real samples analysis.

关键词： Papaya juice-stabilized gold nanoclusters Fluorescent probe Capping agent Urine L-lysine

In-depth investigation on the factors affecting the performance of high oil-absorption resin by response surface method

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Chinese Journal of Chemical Engineering 2021年第5期34卷 286-296页

作者： Lei Ma Hongxia Lv Haonan Yu Lingtong Kong rongyue zhang Xiaoyan Guo Haibo Jin Guangxiang He Xiaoyan Liu Beijing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology College of Chemical EngineeringBeijing Institute of Petrochemical TechnologyBeijing 102617China Water Resource Office of Jining City Shandong ProvinceJining 272019China

A series of high oil-absorption resins with low cross-linking degree were synthesized by suspension polymerization using stearyl methacrylate(SMA),2-Ethylhexyl methacrylate(EHMA),and styrene(St)as *** surface methodology(RSM)with central composite design(CCD)was also applied to determine the optimal parameters that are mainly known to affect their ***,the effects of the monomer mass ratio(EHMA:SMA),the rigid monomer(St)dosage,the porous agent(acetone)dosage,and their pairwise interaction on the resin's oil-absorption capacity were analyzed,highlighting PSES-R_(2) as the resin with the optimum *** pure oil-absorption rates of PSES-R_(2) for gasoline,diesel,and kerosene were 11.19 g·g^(-1),16.25 g·g^(-1),and 14.84 g·g^(-1),respectively,while the oil removal rates from oily wastewater were 98.82%,65.11%,and 99.63%,respectively.

关键词： Oil-absorption resin Response surface method Oil removal rate Waste water Absorption Polymerization

Investigation on Outbreak of Sesamia inferens and Smut in Cane-growing Regions of Yunnan Province

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Agricultural Biotechnology 2023年第2期12卷 63-66,72页

作者： Yongde MA Xiaoyan WANG Yinhu LI Hongli SHAN rongyue zhang Yingkun HUANG Agricultural and Rural Affairs Bureau of Gengma County Gengma 677500China Sugarcane Research Institute Yunnan Academy of Agricultural SciencesKaiyuan 661699China

[Objectives]This study was conducted to clarify the outbreak and disaster characteristics of Sesamia inferens Walker and smut in cane-growing regions in the low-latitude plateau of Yunnan Province,where there is continuous high temperature and drought in spring and summer.[Methods]A systematic investigation and analysis was conducted on different varieties in different types of fields in 16 cane-growing regions with outbreak of *** and smut in Yunnan from 2019 to 2021.[Results]The outbreak of *** and smut in cane-growing regions in the low-latitude plateau of Yunnan Province resulted in an average percent of dry hearts in the range of 17.3%-56.3%,and as high as 84.0%in severe cases.70%thiamethoxam seed treatment dispersible powder and clothianidin or thiamethoxam fertilizer granules were ineffective against ***,while 10%monosultap·thiamethoxam or 10%clothianidin·monosultap granules had a significant control efficacy on *** average diseased plant rate ranged from 14.8%to 89.7%in susceptible varieties with smut,and was as high as 100%in severe *** 1,Yuetang 93-159,Yuetang 00-236,and Liucheng 05-136 had strong resistance,with an average diseased plant rate of 0-5.5%and the highest rate of 7.6%.*** and smut both broke out in cane-growing regions with an altitude below 1200 *** is recommended to pay attention to disease and pest monitoring in cane-growing regions with an altitude below 1200 m,and combine it with the management of new plants and ratoon plants to effectively prevent and control the first and second generation of ***-infested seedlings from March to *** is also recommended to strengthen the promotion and application of excellent disease-resistant varieties,so as to achieve a reasonable distribution of varieties and fundamentally control the outbreak and epidemic of sugarcane smut.[Conclusions]This study provides a basis for the effective prevention and control of catastrophic *** and smut caused

关键词： High temperature Drought Sesamia inferens Smut Outbreak

Detection and Identification of Sugarcane Pokkah Boeng Pathogens of Main Sugarcane Varieties in Yunnan Province

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Agricultural Biotechnology 2022年第6期11卷 43-47页

作者： Xiaoyan WANG Wenfeng LI Yinhu LI Hongli SHAN rongyue zhang Yingkun HUANG Key Laboratory on Genetic Improvement of Sugarcane in Yunnan Province Sugarcane Research InstituteYunnan Academy of Agricultural SciencesKaiyuan 661699China

[Objectives]This study was conducted to identify the pathogen species and dominant species of sugarcane pokkah boeng in main varieties in Yunnan sugarcane areas,so as to promote the healthy and sustainable development of sugarcane industry.[Methods]Specific primers Fv-F3/Fv-R3 and Fp-F4/Fp-R4 were designed based on the ribosomal DNA non-internal transcribed spacer(rDNA-ITS)gene sequences of Fusarium verticillioides and Fusarium proliferarum,the main pathogens of sugarcane pokkah boeng,and 117 typical sugarcane pokkah boeng samples collected from main varieties in different sugarcane areas of Yunnan Province were detected and analyzed by PCR.[Results]Among the 117 sugarcane pokkah boeng samples,112 samples were detected with *** with a positive detection rate of 95.7%;103 samples were detected with *** with a positive detection rate of 88%;103 samples were infected by ***+***,and the compound infection rate was 88%;and the two pathogens were not detected in 5 samples,which might be sugarcane pokkah boeng caused by other *** amplification products of 23 *** positive samples and 19 *** positive samples from different sugarcane varieties in different sugarcane areas were *** BLAST alignment results showed that the sequences of the 23 amplification products of *** shared 99.45%-100.00%homology with ***(GenBank accession number:KU508286),and the sequences of the 19 amplification products of *** shared 99.26%-100.00%homology with the sequence of ***(GenBank accession number:MK252904).Part of the *** and 11 *** sequences were selected to construct a phylogenetic tree,and the phylogenetic analysis showed that they belonged to the *** group and *** group,*** results showed that *** and *** were the main pathogens causing sugarcane pokkah boeng of the

关键词： Sugarcane pokkah boeng Pathogens Dominant species Main varieties PCR detection Phylogenetic analysis

Efficacy of High-efficiency Formula Pesticides for Controlling Sesamia inferens Walker and Ceratovacuna lanigera Zehntner

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Agricultural Biotechnology 2023年第2期12卷 42-44,47页

[Objectives]This study was conducted to screen high-efficiency formula pesticides and precise and efficient application techniques for the prevention and control of Sesamia inferens Walker and Ceratovacuna lanigera Zehntner.[Methods]Field efficacy trials were conducted using different combinations of 70%directed enhanced thiamethoxam seed treatment dispersible powder and 46%monosultap·Bacillus thuringiensis wettable powder.[Results]70%directed enhanced thiamethoxam ZF 450 g/hm^(2)+46%monosultap·*** WP 2250 g/hm^(2)had good control effects on both *** and ***,so it was an ideal high-efficiency formula pesticide for controlling sugarcane *** and *** January to May,combining with new plant or perennial root cultivation management,70%directed enhanced thiamethoxam ZF 450 g/hm^(2)can be evenly spread on sugarcane ditches and sugarcane stumps after being well mixed with fertilizers applied per hectare,and covered with soil in a timely manner,and during the peak incubation period of the first and second generation of *** eggs from March to May,46%monosultap·*** WP 2250 g/hm^(2)was sprayed to the leaf surface in the mixture with water 675 kg with an electric backpack or a motorized *** such a way,the control efficacy on dead heart seedlings could reach over 89.8%,and that on *** could reach 100%.Meanwhile,it could effectively and concurrently control thrips,and the increases in yield and sugar content could reach 29310 kg/hm^(2)and 5.9 percentage points,respectively.[Conclusions]The promotion and application of the formula pesticide could achieve precise application and control of sugarcane pests,and improvement of sugarcane quality and planting efficiency,and help promote green prevention and control of sugarcane diseases and pests and the sustained high-quality development of the sugar industry.

关键词： Sugarcane Efficient formula pesticide Accurate application of pesticides Sesamia inferens Walker Ceratovacuna lanigera Zehntner Control efficacy evaluation

Establishment and Application of Identification Methods for Resistance to Sugarcane White Leaf Disease(SCWL)

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Agricultural Biotechnology 2023年第1期12卷 12-15页

作者： Wenfeng LI rongyue zhang Xiaoyan WANG Hongli SHAN Jie LI Yinhu LI Yingkun HUANG Key Laboratory on Genetic Improvement of Sugarcane in Yunnan Province Sugarcane Research InstituteYunnan Academy of Agricultural SciencesKaiyuan 661699China

[Objectives]This study was conducted to establish simple, efficient, stable, standardized and practical identification methods for sugarcane resistance to white leaf disease(SCWL), and promote the breeding for sugarcane resistance to SCWL. [Methods]The identification technology of sugarcane resistance to SCWL was systematically studied and explored from the aspects of sugarcane material treatment and planting, inoculation liquid preparation, inoculation method, disease investigation, grading standard formulation, etc., and two sets of simple, efficient, stable, standardized and practical accurate identification methods for sugarcane resistance to SCWL were created for the first time, namely, the seed cane coating inoculation method and the stem-cutting inoculation method at the growth stage. The seed cane coating inoculation method includes the steps of directly screening SCWL phytoplasma, extracting juice from cane and adding 10 times of sterile water to prepare an inoculation liquid, spraying seed cane on plastic film to keep moisture, planting the inoculated materials in barrels in an insect-proof greenhouse for cultivation, investigating the incidence rate 30 d after inoculation, and evaluating the disease resistance according to the 1-5 level standard. The method of stem-cutting inoculation includes the steps of directly screening sugarcane stems carrying SCWL phytoplasma and adding 10 times of sterile water to prepare an inoculation liquid, cultivating the identification materials in an insect-proof greenhouse, dropping 100 μl of the inoculation liquid into each root incision with a pipette gun at the age of 6 months, investigating the incidence rate 20 d after planting, and evaluating the disease resistance according to the 1-5 level standard. [Results] The two methods are similar to the natural transmission method. After inoculation, SCML occurred significantly, with high sensitivity and good reproducibility. The results of resistance identification were con

关键词： Sugarcane white leaf disease Inoculation technology Seed cane-spraying inoculation Stem-cutting inoculation method Identification of disease resistance

Research Progresses of Sugarcane Brown Rust Resistance Gene and Molecular Markers

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Plant Diseases and Pests 2017年第3期8卷 25-28页

作者： Cang Xiaoyan Huang Yingkun Wang Xiaoyan Shan Hongli zhang rongyue Li Wenfeng Sugarcane Research Institute Yunnan Academy of Agricultural SciencesYunnan Provincial Key Laboratory of Sugarcane Genetic Improvement

Brown rust is a main sugarcane disease in China, affecting stable development of sugarcane industry. Resistance breeding is an effective measure to control sugarcane rust, and looking for diverse sugarcane rust resistance alternative resources is another important way to improve rust resistance level. This article reviewed from the aspects of construction of sugarcane genetic map, research progress of rust resistance gene, development of rust resistance molecular markers, and research strategies of rust resistance gene, in order to provide scientific guidance and reference for rust resistance breeding.

关键词： Sugarcane Brown rust Resistance breeding Resistance gene Brul Molecular markers

Evaluation of Control Effects against Sugarcane Pests：A New Sustained-release and Long-lasting Pesticide and Convenient and Efficient Pesticide Application Technology

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Plant Diseases and Pests 2017年第5期8卷 11-14页

作者： Li Wenfeng Pu Chunhua zhang rongyue Yin Jiong Luo Zhiming Shan Hongli Wang Xiaoyan Huang Yingkun Sugarcane Research Institute Yunnan Academy of Agricultural Sciences/Yunnan Provincial Key Laboratory of Sugarcane Genetic Improvement Kaiyuan 616699 China Yunnan Academy of Agricultural Science Kunming 650205 China

[Objective]The paper was to screen a new ideal sustained-release,long-lasting and low-toxic pesticide and convenient and efficient pesticide application technology for controlling Ceratovacuna lanigera and Baliathrips serratus. [Method]2% Imidacloprid GR were selected and applied in the soil for field efficacy trial. [Result] The optimum dosage of 2% imidacloprid GR was 30 kg/hm^2（ active ingredient 600 g）,which can be mixed with fertilizer（ 30 kg pesticide and 40-80 kg fertilizer per hm^2） once combined with sugarcane planting management or big ridging during February and June. The control effects against C. lanigera and B. serratus could be more than 98. 2% and 81. 1%,respectively. The actual yield and sugar content in various pesticide treatments were increased by 33 390 kg/hm^2 and 6. 6% respectively compared to blank control. [Conclusion]2% imidacloprid GR has good control effects on C. lanigera and B. serratus. It is a new pesticide with ideal sustained-release,long-lasting and low-toxin effects against C. lanigera and B. serratus. Therefore,it could be used alternatively with other pesticides,to delay production and development of drug resistance.

关键词： Sustained-release and long-lasting pesticide Convenient and efficient pesticide application Sugarcane pests Control effect evaluation

Detection,Identification and Phylogenetic Analysis of Sugarcane Pokkah Boeng in Yunnan Sugarcane Areas

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Agricultural Biotechnology 2022年第4期11卷 22-25页

作者： Xiaoyan WANG Xiaoyan CANG Wenfeng LI Yinhu LI Hongli SHAN rongyue zhang Yingkun HUANG Sugarcane Research Institute Yunnan Academy of Agricultural SciencesKaiyuan 661699China

[Objectives]This study was conducted to determine the pathogen species and dominant species of sugarcane pokkah boeng in the sugarcane areas of Yunnan in the low-latitude plateau,so as to provide a basis for the pathogenic mechanism,disease resistance breeding and scientific prevention and control of pokkah boeng.[Methods]With 14 pokkah boeng samples collected from different sugarcane areas in Yunnan as materials,pokkah boeng-specific detection primers FvF4/Fv-R4 and Fp-F3/Fp-R3 were designed based on ribosomal DNA non-transcribed spacer(rDNA-ITS)sequences,respectively,and used to perform PCR on Fusarium verticillioides and ***.[Results]In 12 of the 14 pokkah boeng samples,two species of pokkah boeng pathogens were detected,and there was a phenomenon of composite *** composite infection samples were selected for sequence alignment and phylogenetic *** sequences of ***(Gen Bank accession number:MZ126549-MZ126555)and ***(Gen Bank accession number:MZ102259-MZ102265)from seven composite infection samples shared 98.6%-100%and 100%with *** strain 20(Gen Bank accession number:KU508286)and *** Dehong strain(GenBank accession number:KJ629482)published in Gen Bank,*** phylogenetic tree showed that the pathogens of sugarcane pokkah boeng in Yunnan were mainly divided into the *** group and *** *** the *** group,except for ROC 25(Lancang,Yunnan)and Funong10-1405(Maitreya,Yunnan)which were on an independent branch,the remaining five composite infection samples were grouped with *** strains from different geographical origins,and were closely related to *** Guangxi *** strains of *** from different geographic origins were clustered into another group.[Conclusions]This study provides a scientific basis for disease resistance breeding and disease prevention and control of sugarcane pokkah boeng.

关键词： Sugarcane Pokkah boeng Dominant species Phylogenetic analysis