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The enhanced genomic 6mA metabolism contributes to the proliferation and migration of TSCC cells

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International Journal of Oral Science 2022年第1期14卷 100-112页

作者： Lei xi ying Yang ying Xu Fangming zhang Jinghui Li xiyang Liu Zhenxi zhang Quan Du State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking UniversityBeijingChina Department of Stomatology Beijing Friendship HospitalCapital Medical UniversityBeijingChina

In contrast to the well-established genomic 5-methylcytosine(5mC),the existence of N^(6)-methyladenine(6 mA)in eukaryotic genomes was discovered only *** studies found that it was actively regulated in cancer cells,suggesting its involvement in the process of ***,the contribution of 6 mA in tongue squamous cell carcinoma(TSCC)still remains *** this study,a pan-cancer type analysis was first performed,which revealed enhanced 6mA metabolism in diverse cancer *** study was then focused on the regulation of 6 mA metabolism,as well as its effects on TSCC *** these aspects,genome 6mA level was found greatly increased in TSCC tissues and cultured *** knocking down 6mA methylases N6AMT1 and METTL4,the level of genomic 6mA was decreased in TSCC *** led to suppressed colony formation and cell *** contrast,knockdown of 6mA demethylase ALKBH1 resulted in an increased 6 mA level,enhanced colony formation,and cell *** study suggested that regulation of the NF-κB pathway might contribute to the enhanced migration of TSCC ***,in the case of TSCC,we have shown that genomic 6mA modification is involved in the proliferation and migration of cancer cells.

关键词： metabolism involvement cancer

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农地流转的碳减排效应研究

西部论坛 2024年第4期34卷 111-124页

作者：张应良崔超西南大学经济管理学院重庆400715 西南大学农村经济与管理研究中心重庆400715

农地流转可以促进农地的集中使用和规模化经营,会降低农业生产经营过程中的化学品和机械等生产性投入强度,并促进农作物种植结构“趋粮化”,进而降低农业碳排放强度。采用2011—2021年30个省份的数据检验农地流转率对农业碳排放强度的影... 详细信息

农地流转可以促进农地的集中使用和规模化经营,会降低农业生产经营过程中的化学品和机械等生产性投入强度,并促进农作物种植结构“趋粮化”,进而降低农业碳排放强度。采用2011—2021年30个省份的数据检验农地流转率对农业碳排放强度的影响,结果表明:农地流转率提高对农业碳排放强度具有显著的负向影响;农地流转能够通过减少农业化学品和机械投入强度、提高粮食种植面积占比来降低农业碳排放强度。进一步分析发现,因流转对象和形式的不同,农地流转的碳减排效应存在异质性,表现为:相比流入农户、企业的农地流转,流入合作社的农地流转具有更为显著的碳减排效应;相比采用出租转包、互换转让形式的农地流转,采用股份合作形式的农地流转具有更为显著的碳减排效应。因此,应积极推动农地有序流转,不断完善农地流转机制,合理引导农地流转方向,并鼓励农地流转与农业碳减排项目相结合,以有效减少农业碳排放。

关键词：农地流转农业碳排放农业化学品投入农业机械投入种植结构农地流转对象农地流转形式

Upregulation of α-ENaC induces pancreatic β-cell dysfunction,ER stress,and SIRT2 degradation

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Journal of Biomedical Research 2024年第3期38卷 241-255页

作者： Xue zhang Dan zhang Lei Huo xin Zhou Jia zhang Min Li Dongming Su Peng Sun Fang Chen xiubin Liang Department of Pathophysiology Nanjing Medical UniversityNanjingJiangsu 211166China Department of Pathology Nanjing Drum Tower Hospitalthe Affiliated Hospital of Nanjing University Medical SchoolNanjingJiangsu 210009China Key Laboratory of Human Functional Genomics of Jiangsu Province Department of Biochemistry and Molecular BiologyNanjing Medical UniversityNanjingJiangsu 211166China Department of Pathology Nanjing Medical UniversityNanjingJiangsu 211166China

Islet beta cells(β-cells)produce insulin in response to high blood glucose levels,which is essential for preserving glucose ***-gated ion channels inβ-cells,including Na+,K+,and Ca2+channels,aid in the release of *** epithelial sodium channel alpha subunit(α-ENaC),a voltage-independent sodium ion channel,is also expressed in human pancreatic endocrine ***,there is no reported study on the function of ENaC in theβ-*** the current study,we found thatα-ENaC was expressed in human pancreatic glandule and pancreatic isletβ-*** the pancreas of db/db mice and high-fat diet-induced mice,and in mouse isletβ-cells(MIN6 cells)treated with palmitate,α-ENaC expression was ***α-ENaC was overexpressed in MIN6 cells,insulin content and glucose-induced insulin secretion were significantly *** the other hand,palmitate injured isletβ-cells and suppressed insulin synthesis and secretion,but increasedα-ENaC expression in MIN6 ***,α-ENaC knockout(Scnn1a−/−)in MIN6 cells attenuatedβ-cell disorder induced by ***,α-ENaC regulated the ubiquitylation and degradation of sirtuin 2 inβ-cells.α-ENaC also modulatedβ-cell function in correlation with the inositol-requiring enzyme 1 alpha/X-box binding protein 1(IRE1α/XBP1)and protein kinase RNA-like endoplasmic reticulum kinase/C/EBP homologous protein(PERK/CHOP)endoplasmic reticulum stress *** results suggest thatα-ENaC may play a novel role in insulin synthesis and secretion in theβ-cells,and the upregulation ofα-ENaC promotes isletβ-cell *** conclusion,α-ENaC may be a key regulator involved in isletβ-cell damage and a potential therapeutic target for type 2 diabetes mellitus.

关键词： α-ENaC pancreaticβ-cells type 2 diabetes mellitus endoplasmic reticulum stress sirtuin 2

L-theanine/α-Ketoglutarate/Nrf2 axis promotes exercise-mediated amelioration of NASH in mice

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Food Science and Human Wellness 2024年

作者： Wang-Jing Mu Min Chen Jie-ying Zhu Hong-Yang Luo Yang Li Shan Li Lin-jing Yan Ruo-ying Li Liang Guo School of Exercise and Health and Collaborative Innovation Center for Sports and Public Health， Shanghai University of Sport Shanghai Frontiers Science Research Base of Exercise and Metabolic Health， Shanghai University of Sport Key Laboratory of Exercise and Health Sciences of the Ministry of Education， Shanghai University of Sport

L-Theanine (LTA) is a non-protein amino acid mainly found in tea plants with many beneficial effects. Exercise exerts a wide range of benefits in metabolic health. Here， we show that exercise or gastric lavage intervention on mice with LTA improves diet-induced NASH in mice. Meanwhile， combinatory therapy shows that exercise and LTA synergistically improve obesity-related metabolic disorders and NASH phenotypes， including hepatic steatosis， inflammation， cell death and oxidative stress. In vivo studies indicate that LTA inhibits free fatty acid (FFA)-induced hepatocyte injury， including steatosis， oxidative stress and apoptosis. Knockdown of Nrf2 blunts the role of LTA in inhibiting FFA-induced hepatocyte oxidative stress and dysfunction. Mechanistically， LTA increases the α-ketoglutarate (α-KG) level in hepatocytes， which increases the transcription of Nrf2 by inducing active DNA demethylation on its promoter. Moreover， LTA promote the above α-KG/Nrf2 axis in synergy with exercise， thereby more efficiently inhibiting hepatic oxidative stress and ameliorating diet-induced NASH in mice. Our results suggest that， through promoting the α-KG/Nrf2 axis-mediated anti-oxidative pathway， the combination of LTA and exercise may provide an effective measure for the prevention and control of NASH.

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Experimental and simulation study on shear stress-induced erythrocyte damage based on vortex oscillator

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Biomedical Engineering Communications 2024年第1期3卷 23-30页

作者： Xu Mei Li-Pu Zhao Lian Hou ying-ying Zhong State Key Laboratory of Digital Medical Engineering School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China School of Mechanical and Electrical Engineering Silicon Lake Vocational and Technical CollegeSuzhou 215006China College of Education Central China Normal UniversityWuhan 430079China

Background:Shear stress-induced erythrocyte damage,namely hemolysis,is an important problem in the development of blood-contacting medical devices such as mechanical circulatory support *** fluid dynamics simulation combined with hemolysis prediction models have been widely used to predict *** the development of hemolysis prediction models,the new hemolysis prediction model requires more experimental data to *** addition,the difference of in vitro blood-shearing device also affect the accuracy of hemolysis ***:To address these problems,a new in vitro blood-shearing device(vortex oscillator)was used to further verify the accuracy of the hemolysis prediction models,and to guide the optimal design of blood-contacting medical devices such as mechanical circulatory support ***,the flow field information such as wall stress and velocity of the vortex oscillator under different speeds was ***,different hemolysis prediction models were used to calculate hemolysis,and the predicted data was compared with the experimental *** and Conclusion:In this study,the flow field information inside the vortex oscillator at high rotational speeds was systematically investigated,and the prediction of hemolysis was carried *** results showed that the predicted data of hemolysis was significantly different from the experimental data,which indicated that it was urgent to establish a standardized in vitro blood-shearing platform to provide a reference for accurate hemolysis prediction.

关键词： CFD Hemolysis prediction model Vortex oscillator Shear stress

采空区地表InSAR形变监测与安全稳定性评价

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中国安全科学学报 2024年第1期34卷 140-149页

作者：董建军张莹李昕梅媛辽宁工程技术大学安全科学与工程学院辽宁葫芦岛125105 辽宁工程技术大学矿山热动力灾害与防治教育部重点实验室辽宁葫芦岛125105

为探明河南省平顶山市某变电站拟建场地的安全稳定性,避免采空区地表沉降或倾斜给变电站造成安全隐患,采用永久散射体(PS)合成孔径雷达干涉测量(InSAR)研究平顶山市2015—2022年间的45景Sentinel-1A数据,精细化分析拟建场地的9个代表位... 详细信息

为探明河南省平顶山市某变电站拟建场地的安全稳定性,避免采空区地表沉降或倾斜给变电站造成安全隐患,采用永久散射体(PS)合成孔径雷达干涉测量(InSAR)研究平顶山市2015—2022年间的45景Sentinel-1A数据,精细化分析拟建场地的9个代表位置的时序形变特征,进而建立基于InSAR监测的采空区地表场地安全稳定性评价机制,完成对场地的安全稳定性评价。研究结果表明:基于模糊数据集的模糊PS选点法可克服PS点密度低的弊端,有效增加分析可用数据,提升监测结果的准确性;依据基准点校正拟建区内代表点的时序形变后发现,拟建场地整体形变较小且逐渐呈现趋于稳定的趋势,最大沉降量为13.05 mm,最大沉降速度为5.73 mm/a,最大倾斜为0.070 mm/m。基于安全稳定性评价机制分析可知:采空区地表场地移动变形处于稳定状态,变电站地基基础处于安全状态,采空区地表沉降对拟建变电站的影响程度小,综合3种评价指标进行分析,采空区地表场地安全稳定性等级为高,具备建设变电站的可行性。

关键词：采空区地表形变永久散射体(PS)合成孔径雷达干涉测量(InSAR) 形变监测安全稳定性评价

2021-2022年江苏省母血清学产前筛查室内质控数据的室间比对结果分析

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中华预防医学杂志 2024年第3期58卷 358-363页

作者：张颖段红蕾李洁贾支俊南京大学医学院附属鼓楼医院产前诊断中心南京210008 南京大学医学院附属鼓楼医院核医学科南京210008

本研究分析2021-2022年江苏省开展母血清学产前筛查的实验室上报的相关数据,通过各实验室室内质控数据的比对,进行江苏省内产前血清学筛查的质量评价。采用江苏省产前筛查质控分析平台收集2021-2022年江苏省参加产前筛查质控的各实验室... 详细信息

本研究分析2021-2022年江苏省开展母血清学产前筛查的实验室上报的相关数据,通过各实验室室内质控数据的比对,进行江苏省内产前血清学筛查的质量评价。采用江苏省产前筛查质控分析平台收集2021-2022年江苏省参加产前筛查质控的各实验室早孕期和中孕期母血清学筛查质控的测定方法、3个浓度的质控数据结果、仪器平台和试剂等相关信息。首先对各实验室检测结果进行统计分析,通过室内质控的变异系数对各实验室的精密度进行评价;其次对使用不同实验平台的实验室进行室间比对,评价其一致性。结果显示,2021年和2022年妊娠相关蛋白-A(PAPP-A)检测变异系数满足行业要求的实验室比例分别为87.5%(7/8)和92.3%(12/13);2021年和2022年游离人绒毛膜促性腺激素(Free β-hCG)(早孕)检测变异系数满足行业要求的实验室比例为97.5%(39/40)和95.5%(42/44),2021年和2022年甲胎蛋白(AFP)检测变异系数满足行业要求的实验室比例分别为100%(40/40)和90.9%(40/44),2021年和2022年游离 β-hCG(中孕)检测变异系数满足行业要求的实验室比例分别为100%(40/40)和95.5%(42/44);各指标的室间比对一致性为100%。综上,江苏省母血清学产前筛查具有较高一致性,对于少部分稳定性较差的实验室需根据数据分析结果做出周期性的评估及整改,完善母血清学产前筛查质量控制管理。

关键词：血清学筛查质量控制室间比对分析

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基于TJS工法的盾构隧道运营变形控制

浙江大学学报（工学版） 2024年第7期58卷 1427-1435页

作者：周思剑张迪周建李瑛龚晓南浙江大学滨海和城市岩土工程研究中心浙江杭州310058 浙江大学城市地下空间开发工程技术研究中心浙江杭州310058 中铁第四勘察设计院集团有限公司湖北武汉430063 浙江省建筑设计研究院浙江杭州310006

开展隧道洞内全方位微扰动高压喷射注浆(TJS)工法对运营隧道变形控制的研究.基于典型区间运营隧道实测数据,采用Plaxis 3D有限元数值模拟软件分析TJS工法横断面布置中桩长、打设角度、桩径对隧道加固效果的影响,通过在隧道底部打设局部... 详细信息

开展隧道洞内全方位微扰动高压喷射注浆(TJS)工法对运营隧道变形控制的研究.基于典型区间运营隧道实测数据,采用Plaxis 3D有限元数值模拟软件分析TJS工法横断面布置中桩长、打设角度、桩径对隧道加固效果的影响,通过在隧道底部打设局部垫层的方法优化TJS工法.结果表明,桩长越长、角度越接近40°、桩径越大,对隧道沉降的控制效果越明显. TJS工法加固后的隧道地表沉降减少16.38 mm,水平收敛变形为未加固时的42.87%.局部垫层能够显著提升TJS桩的加固效果,“两侧短中间长”的布置能够兼顾加固效果和降低施工成本.

关键词： TJS工法洞内加固盾构隧道运营变形控制数值模拟

Rotating magnetic field inhibits Aβ protein aggregation and alleviates cognitive impairment in Alzheimer’s disease mice

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Zoological Research 2024年第4期45卷 924-936页

作者： Ruo-Wen Guo Wen-Jing xie Biao Yu Chao Song xin-Miao Ji xin-Yu Wang Mei zhang xin zhang High Magnetic Field Laboratory CAS Key Laboratory of High Magnetic Field and Ion Beam Physical BiologyHefei Institutes of Physical ScienceChinese Academy of SciencesHefeiAnhui 230031China Science Island Branch of Graduate School University of Science and Technology of ChinaHefeiAnhui 230036China Institutes of Physical Science and Information Technology Anhui UniversityHefeiAnhui 230601China School of Life Sciences Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui 230026China

Amyloid beta(Aβ)monomers aggregate to form fibrils and amyloid plaques,which are critical mechanisms in the pathogenesis of Alzheimer’s disease(AD).Given the important role of Aβ1-42 aggregation in plaque formation,leading to brain lesions and cognitive impairment,numerous studies have aimed to reduce Aβaggregation and slow AD *** diphenylalanine(FF)sequence is critical for amyloid aggregation,and magnetic fields can affect peptide alignment due to the diamagnetic anisotropy of aromatic *** this study,we examined the effects of a moderate-intensity rotating magnetic field(RMF)on Aβaggregation and AD *** indicated that the RMF directly inhibited Aβamyloid fibril formation and reduced Aβ-induced cytotoxicity in neural cells in *** the AD mouse model APP/PS1,RMF restored motor abilities to healthy control levels and significantly alleviated cognitive impairments,including exploration and spatial and non-spatial memory *** examinations demonstrated that RMF reduced amyloid plaque accumulation,attenuated microglial activation,and reduced oxidative stress in the APP/PS1 mouse *** findings suggest that RMF holds considerable potential as a non-invasive,high-penetration physical approach for AD treatment.

关键词： lzheimer’s disease Rotating magnetic field Amyloid-β Cognitive function Alzheimer’s disease animal models