检索结果-南通市图书馆

Metabolic properties of chicken embryonic stem cells

Science China(Life Sciences) 2010年第9期53卷 1073-1084页

作者： LI Jia zhang baolu HAN HongBing CAO ZhiCheng LIAN ZhengXing LI Ning State Key Laboratory of Agrobiotechnology China Agricultural UniversityBeijing 100194China College of Animal Science&Technology China Agricultural UniversityBeijing 100194China

Cellular energy metabolism correlates with cell fate,but the metabolic properties of chicken embryonic stem (chES) cells are poorly *** a previously established chES cell model and electron microscopy (EM),we found that undifferentiated chES cells stored ***,undifferentiated chES cells expressed lower levels of glucose transporter 1 (GLUT1) and phosphofructokinase (PFK) mRNAs but higher levels of hexokinase 1 (HK1) and glycogen synthase (GYS) mRNAs compared with control primary chicken embryonic fibroblast (CEF) cells,suggesting that chES cells direct glucose flux towards the glycogenic ***,we demonstrated that undifferentiated chES cells block gluconeogenic outflow and impede the accumulation of glucose-6-phosphate (G6P) from this pathway,as evidenced by the barely detectable levels of pyruvate carboxylase (PCX) and mitochondrial phosphoenolpyruvate carboxykinase (PCK2) ***,cell death occurred in undifferentiated chES cells as shown by Hoechst 33342 and propidium iodide (PI) double staining,but it could be rescued by exogenous ***,we found that differentiated chES cells decreased the glycogen reserve through the use of PAS ***,differentiated chES cells expressed higher levels of GLUT1,HK1 and PFK mRNAs,while the level of GYS mRNA remained similar in control CEF *** data indicate that undifferentiated chES cells continue to synthesize glycogen from glucose at the expense of G6P,while differentiated chES cells have a decreased glycogen reserve,which suggests that the amount of glycogen is indicative of the chES cell state.

关键词： chicken embryonic stem cell energy metabolism glycogen cell fate glucose-6-phosphate

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

改良跟骨接骨板系统联合缝线锚钉治疗Liu-GangⅣ型肱骨大结节劈裂骨折

引用

中国组织工程研究 2024年第24期28卷 3855-3861页

作者：刘刚张宝露李锐辰侯晓梅罗鸿赖灿豪李青缘梁霞暴丁溯扶世杰曾胜强西南医科大学附属中医医院骨关节·足踝外科四川省泸州市646000 广东省医学3D打印应用转化工程技术研究中心西南医科大学附属中医医院临床基地四川省泸州市646000 西南医科大学护理学院四川省泸州市646000 江安县中医医院四川省宜宾市644200 成都中医药大学临床医学院四川省成都市610000 西南医科大学附属中医医院影像科四川省泸州市646000

背景:既往有许多学者研究单独的肱骨大结节骨折及其手术方式。但是,对于肱骨大结节劈裂骨折(Liu-GangⅣ型)合并肩袖损伤,几乎没有学者进行深入的细化研究。目的:比较切开复位改良的跟骨接骨板系统联合缝线锚钉和PHILOS锁定内固定系统联... 详细信息

背景:既往有许多学者研究单独的肱骨大结节骨折及其手术方式。但是,对于肱骨大结节劈裂骨折(Liu-GangⅣ型)合并肩袖损伤,几乎没有学者进行深入的细化研究。目的:比较切开复位改良的跟骨接骨板系统联合缝线锚钉和PHILOS锁定内固定系统联合强生2#号线治疗肱骨大结节劈裂骨折合并肩袖损伤(Liu-GangⅣ型)的临床疗效。方法:回顾性分析2012年5月至2022年5月30例肱骨大结节劈裂骨折合并肩袖损伤患者(Liu-GangⅣ型)的病历资料,分为改良的跟骨接骨板系统联合缝线锚钉组(A组)和PHILOS锁定内固定系统联合强生2#号线组(B组),每组15例。记录所有患者术中出血量、手术时间和切口长度,术后末次随访(>1年),评估其疼痛目测类比评分、Constant-Murley评分以及肩关节外展、前屈、外旋和背伸活动。结果与结论:①A组手术切口长度、手术时间和出血量均小于B组(P0.05);③术后末次随访A组患者前屈功能优于B组(P0.05);④并发症方面,A组1例肩关节疼痛不适(7%);B组2例肩峰下撞击综合征,2例大结节上移,2例肩关节疼痛(40%),两组并发症发生率相比差异有显著性意义(P=0.031);⑤提示采用改良的跟骨接骨板系统联合缝线锚钉治疗肱骨大结节劈裂骨折(Liu-GangⅣ型),不仅可以更好地恢复肩关节前屈功能,而且手术切口较小、出血量较少、手术时间较短,且并发症较少。

关键词：肱骨大结节劈裂骨折 Liu-GangⅣ型新分型手术治疗改良的跟骨接骨板系统 PHILOS接骨板系统

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

SIRT7 orchestrates melanoma progression by simultaneously promoting cell survival and immune evasion via UPR activation

引用

Signal Transduction and Targeted Therapy 2023年第4期8卷 1953-1969页

作者： Xiuli Yi Huina Wang Yuqi Yang Hao Wang Hengxiang zhang Sen Guo Jianru Chen Juan Du Yangzi Tian Jingjing Ma baolu zhang Lili Wu Qiong Shi Tianwen Gao Weinan Guo Chunying Department of Dermatology Xijing HospitalFourth Military Medical UniversityNo 127 of West Changle RoadXi’anShaanxi 710032China

Melanoma is the most lethal type of skin cancer,originating from the malignant transformation of *** the development of targeted therapy and immunotherapy has gained revolutionary advances in potentiating the therapeutic effect,the prognosis of patients with melanoma is still *** tumor progression,melanoma frequently encounters stress from both endogenous and exogenous sources in tumor ***7 is a nuclear-localized deacetylase of which the activity is highly dependent on intracellular nicotinamide adenine dinucleotide(NAD+),with versatile biological functions in maintaining cell ***,whether SIRT7 regulates tumor cell biology and tumor immunology in melanoma under stressful tumor microenvironment remains ***,we reported that SIRT7 orchestrates melanoma progression by simultaneously promoting tumor cell survival and immune evasion via the activation of unfolded protein *** first identified that SIRT7 expression was the most significantly increased one in sirtuins family upon ***,we proved that the deficiency of SIRT7 potentiated tumor cell death under stress in vitro and suppressed melanoma growth in ***,SIRT7 selectively activated the IRE1α-XBP1 axis to potentiate the pro-survival ERK signal pathway and the secretion of tumorpromoting ***7 directly de-acetylated SMAD4 to antagonize the TGF-β-SMAD4 signal,which relieved the transcriptional repression on IRE1αand induced the activation of the IRE1α-XBP1 ***,SIRT7 up-regulation eradicated anti-tumor immunity by promoting PD-L1 expression via the IRE1α-XBP1 ***,the synergized therapeutic effect of SIRT7 suppression and anti-PD-1 immune checkpoint blockade was also *** together,SIRT7 can be employed as a promising target to restrain tumor growth and increase the effect of melanoma immunotherapy.

关键词： melanoma SIRT7 immunity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Over-expression of Toll-like receptor 2 up-regulates heme oxygenase-1 expression and decreases oxidative injury in dairy goats

引用

Journal of Animal Science and Biotechnology 2017年第2期8卷 351-360页

作者： Shoulong Deng Kun Yu Wuqi Jiang Yan Li Sutian Wang Zhuo Deng Yuchang Yao baolu zhang Guoshi Liu Yixun Liu Zhengxing Lian State Key Laboratory of Stem Cell and Reproductive Biology Institute of Zoology Chinese Academy of SciencesBeijing 100101 China Laboratory ofAnimal Genetics and Breeding College of Animal Science and TechnologyChina Agricultural University Beijing 100193 People's Republic of China National key Lab of Agrobiotechnology College of Biological SciencesChina Agricultural University Beijing 100193 People's Republic of China. Department of Animal Science Oklahoma State University Stillwater OK74078 USA College of Animal Science and Technology Northeast Agricultural University Harbin 150030 People's Republic of China State Oceanic Administration Beijing 100860 People's Republic of China

Background： Mastitis, an infection caused by Gram-positive bacteria, produces udder inflammation and oxidative injury in milk-producing mammals. Toll-like receptor 2（TLR2） is important for host recognition of invading Grampositive microbes. Over-expression of TLR2 in transgenic dairy goats is a useful model for studying various aspects of infection with Gram-positive bacteria, in ***： We over-expressed TLR2 in transgenic dairy goats. Pam3CSK4, a component of Gram-positive bacteria,triggered the TLR2 signal pathway by stimulating the monocytes-macrophages from the TLR2-positive transgenic goats, and induced over-expression of activator protein-1（AP-1）, phosphatidylinositol 3-kinase（PI3K） and transcription factor nuclear factor kappa B（NF-κB） and inflammation factors downstream of the signal ***： Compared with wild-type controls, measurements of various oxidative stress-related molecules showed that TLR2, when over-expressed in transgenic goat monocytes-macrophages, resulted in weak lipid damage, high level expression of anti-oxidative stress proteins, and significantly increased m RNA levels of transcription factor NF-E2-related factor-2（Nrf2） and the downstream gene, heme oxygenase-1（HO-1）. When Pam3CSK4 was used to stimulate ear tissue in vivo the HO-1 protein of the transgenic goats had a relatively high expression ***： The results indicate that the oxidative injury in goats over-expressing TLR2 was reduced following Pam3CSK4 stimulation. The underlying mechanism for this reduction was increased expression of the anti-oxidation gene HO-1 by activation of the Nrf2 signal pathway.

关键词： Haem oxygenase Nrf2 signal pathway Toll-like receptor 2 Transgenic goats

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Nitric oxide in the development of visual cortex of golden hamster

Nitric oxide in the development of visual cortex of golden h...

引用

The Third Asian Conference on Vision (ACV2004)

作者： Yueting zhang Jie zhang Deliang zhang and baolu Zhao Institute of Biophyisc Academia SInica Beijing 100101 China

＜正＞ Apoptosis is considered as a morphogenetic process that allows for the elimination of cells that have been either produced in excess or completed their functional roles. NO has been shown to influence neurotransmitter release and synaptogenic processes, modulate the synaptic plasticity, indicating that NO may play an important role in the development, maintenance and regulation of brain circuit.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Germ cell-specific deletion of Pex3 reveals essential roles of PEX3-dependent peroxisomes in spermiogenesis

引用

The Journal of Biomedical Research 2024年第1期38卷 24-36页

作者： Yejin Yao baolu Shi Xiangzheng zhang Xin Wang Shuangyue Li Ying Yao Yueshuai Guo Dingdong Chen Bing Wang Yan Yuan Jiahao Sha Xuejiang Guo State Key Laboratory of Reproductive Medicine and Offspring Health Nanjing Medical UniversityNanjingJiangsu 211166China Reproductive and Genetic Branch the First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui 230001China State Key Laboratory of Reproductive Medicine and Offspring Health Women's Hospital of Nanjing Medical UniversityNanjing Maternity and Child Health Care HospitalNanjing Medical UniversityNanjingJiangsu 211166China.

Peroxisomes are organelles enclosed by a single membrane and are present in various *** abruption of peroxisomes is correlated with peroxisome biogenesis disorders and single peroxisomal enzyme deficiencies that induce diverse diseases in different ***,little is known about the protein compositions and corresponding roles of heterogeneous peroxisomes in various *** transcriptomic and proteomic analyses,we observed heterogenous peroxisomal components among different organs,as well as between testicular somatic cells and different developmental stages of germ *** Pex3 is expressed in both germ cells and Sertoli cells,we generated Pex3 germ cell-and Sertoli cell-specific knockout *** Pex3 deletion in Sertoli cells did not affect spermatogenesis,the deletion in germ cells resulted in male sterility,manifested as the destruction of intercellular bridges between spermatids and the formation of multinucleated giant *** analysis of the Pex3-deleted spermatids revealed defective expressions of peroxisomal proteins and spermiogenesis-related *** findings provide new insights that PEX3-dependent peroxisomes are essential for germ cells undergoing spermiogenesis,but not for Sertoli cells.

关键词： male infertility spermiogenesis peroxisome oxidative stress PEX3

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

海岸带遥感研究现状及发展趋势分析

引用

应用海洋学学报 2022年第2期41卷 215-228页

作者：姜璐余静张宝路中国海洋大学海洋与大气学院山东青岛266100 教育部人文社会科学重点研究基地中国海洋大学海洋发展研究院山东青岛266100 自然资源部海洋咨询中心北京100071

系统地探明海岸带遥感的研究现状和发展趋势,对于应对海岸带地区面临的生态系统功能下降、自然岸线损失、水质恶化等问题具有至关重要的意义。为了充分明晰海岸带遥感研究现状及发展趋势,本文以“海岸带遥感”为主题,基于Web of Scienc... 详细信息

系统地探明海岸带遥感的研究现状和发展趋势,对于应对海岸带地区面临的生态系统功能下降、自然岸线损失、水质恶化等问题具有至关重要的意义。为了充分明晰海岸带遥感研究现状及发展趋势,本文以“海岸带遥感”为主题,基于Web of Science数据库,筛选出1990—2019年发表的相关文献共6083篇;从论文发表基本情况、科研合作和研究内容聚焦及主题演变方面进行了较为系统的文献计量评价和知识图谱分析。在文献发表基本情况方面,1990—2019年间海岸带遥感发文量呈现较为稳定的上升趋势;区域性特征明显,主要集中于各大洲沿海地区,发达国家在该领域的研究中占据主流地位;Remote Sensing of Environment是该领域刊文量最高的期刊,且具有最高的影响因子;美国、中国科学院和Hu C M分别为发文量最高的国家、机构和作者;根据H指数的分析结果,相关科研成果的学术影响力仍有待进一步提升。在科研合作方面,美、英等发达国家之间形成了较为紧密的合作网络;虽然中国科学院是发文量最大的科研机构,但是中介中心性仅为0.09,低于美国国家航空航天局(0.16)和美国国家海洋与大气管理局(0.12),因此应进一步加强与其他研究机构的合作;Hu C M与其他学者之间的学术合作最为紧密。在研究内容聚焦及主题演变方面,海岸带遥感领域的研究主题呈现多元化的趋势;研究内容主要聚焦于水色、海岸线、土地利用/覆盖、生态系统监测、灾害和城市化等方面。

关键词：海岸带资源及其开发海岸带遥感文献计量 CiteSpace 知识图谱 H指数

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Anti-senescence effect of green tea catechin EGCG

Anti-senescence effect of green tea catechin EGCG

引用

第十次中国生物物理学术大会

作者： zhang Longze and Zhao baolu State Key Laboratory of Brain and Cognition Science Biophysical Institute of Chinese Academy of Sciences Beijing China

＜正＞Tea consumption is widely believed to be beneficial in association with anti- carcinogenesis, anti-obesity, anti-aging and hypolipidemic effect and blood pressure reduction. Green tea contains various bioactive compounds, such as polyphenol and caffeine, with Epigallocatechin gallate （EGCG） as a main active ingredient. Many studies have provided solid proofs for the anti-cancer and anti-obesity effects of EGCG. However, no wide and

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

井下承压开采水害防治探究

引用

能源与节能 2016年第9期 181-182页

作者：张保录长治市煤炭安全纠察支队山西长治046000

结合A矿工程实际,在分析其地质水害特点的基础上,构建了有针对性的综合化水害防治体系,实现了承压水害的有效控制,实现矿井经济效益与社会效益双赢。

关键词：煤矿承压开采水害防治综合管理防治体系

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

高大模板支架施工技术关键问题分析及处理

引用

铁道建筑 2016年第9期56卷 54-56页

作者：张永春秘红丹师宝禄张小伟中铁建设集团有限公司北京100040 北京交通大学土建学院北京100044

在建筑施工过程中高大模板支架失稳引起架体整体坍塌的事件时有发生。其主要原因有设计计算不规范、施工技术措施不当、监管不严等。本文结合高大模板支架坍塌实例,对国内高大模板支架施工技术的现状及存在的问题进行了归纳和总结,详细... 详细信息

在建筑施工过程中高大模板支架失稳引起架体整体坍塌的事件时有发生。其主要原因有设计计算不规范、施工技术措施不当、监管不严等。本文结合高大模板支架坍塌实例,对国内高大模板支架施工技术的现状及存在的问题进行了归纳和总结,详细地阐述了高大模板支架施工技术要点,建议在此类施工中布设支架整体稳定性监控系统。

关键词：高大模板支架稳定性施工技术监控系统

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：